Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein.

abstract：:The induction of hemoglobin synthesis by dimethylsulfoxide in cells infected with Friend leukemia virus has been shown to depend on the number of cell divisions after the addition of the inducing agent. In asynchronous cultures the cells have a generation time of 24 hr, and hemoglobin synthesis occurs after 48 hr in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClintock PR,Papaconstantinou J

更新日期：1974-11-01 00:00:00

abstract：:The mammalian gastrointestinal tract harbors thousands of bacterial species that include symbionts as well as potential pathogens. The immune responses that limit access of these bacteria to underlying tissue remain poorly defined. Here we show that γδ intraepithelial lymphocytes (γδ IEL) of the small intestine produc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ismail AS,Severson KM,Vaishnava S,Behrendt CL,Yu X,Benjamin JL,Ruhn KA,Hou B,DeFranco AL,Yarovinsky F,Hooper LV

更新日期：2011-05-24 00:00:00

abstract：:In the central nervous system, glycogen-derived bioenergetic resources in astrocytes help promote tissue survival in response to focal neuronal stress. However, our understanding of the extent to which these resources are mobilized and utilized during neurodegeneration, especially in nearby regions that are not active...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cooper ML,Pasini S,Lambert WS,D'Alessandro KB,Yao V,Risner ML,Calkins DJ

更新日期：2020-08-04 00:00:00

abstract：:Within the scientific community, much attention has focused on improving communications between scientists, policy makers, and the public. To date, efforts have centered on improving the content, accessibility, and delivery of scientific communications. Here we argue that in the current political and media environment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iyengar S,Massey DS

更新日期：2019-04-16 00:00:00

abstract：:It is well established that pregnancy early in life reduces the risk of breast cancer in women and that this effect is universal. This phenomenon of parity protection against mammary cancer is also observed in rodents. Earlier studies have demonstrated that short-term administration of estradiol (E) in combination wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rajkumar L,Guzman RC,Yang J,Thordarson G,Talamantes F,Nandi S

更新日期：2001-09-25 00:00:00

abstract：:A methylcholanthrene-induced glioblastoma of the C57BL/6 inbred mouse strain was used to raise antibodies in C57BL/6 and C57BL/10 inbred mice and in (C57BL/6 x DBA/2) and (C57BL/6 x Balb/c) F(1) hybrids. When examined by the cytotoxicity test, these antibodies define a cell-surface component (or components) found excl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schachner M

更新日期：1974-05-01 00:00:00

abstract：:We have previously shown that hepatitis B virus (HBV) replication is controlled by noncytolytic mechanisms that depend primarily on the effector functions of the CD8(+) T cell response, especially the production of IFN-gamma in the liver. The mechanisms that control the nuclear pool of viral covalently closed circular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wieland SF,Spangenberg HC,Thimme R,Purcell RH,Chisari FV

更新日期：2004-02-17 00:00:00

abstract：:Global demand for agricultural products such as food, feed, and fuel is now a major driver of cropland and pasture expansion across much of the developing world. Whether these new agricultural lands replace forests, degraded forests, or grasslands greatly influences the environmental consequences of expansion. Althoug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gibbs HK,Ruesch AS,Achard F,Clayton MK,Holmgren P,Ramankutty N,Foley JA

更新日期：2010-09-21 00:00:00

abstract：:An unusual cAMP signaling system mediates many of the events that prepare spermatozoa to meet the egg. Its components include the atypical, bicarbonate-stimulated, sperm adenylyl cyclase and a cAMP-dependent protein kinase (PKA) with the unique catalytic subunit termed Calpha(2) or C(s). We generated mice that lack Ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nolan MA,Babcock DF,Wennemuth G,Brown W,Burton KA,McKnight GS

更新日期：2004-09-14 00:00:00

abstract：:Stimulation of catecholamine release was studied in the isolated adrenal of the cat during retrograde perfusion. Theophylline, adenosine 3':5'-cyclic monophosphate (cyclic AMP), and dibutyryl-cyclic AMP stimulated catecholamine release in adrenal chromaffin tissue; adenosine, 2'-AMP, 3'-AMP, and 5'-AMP were ineffectiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peach MJ

更新日期：1972-04-01 00:00:00

abstract：:We have carried out partial amino acid sequence analysis of a putative nuclear pore complex protein (nucleoporin) of rat that reacts with wheat germ agglutinin and with the polyspecific monoclonal antibody 414. Surprisingly, these partial amino acid sequence data revealed a high degree of similarity with the human CAN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kraemer D,Wozniak RW,Blobel G,Radu A

更新日期：1994-02-15 00:00:00

abstract：:The cellular response to ionizing radiation (IR) includes induction of the c-jun and EGR1 early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. The results demonstrate activation of the 40S ribosomal protein S6 kinase, pp90rsk, in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kharbanda S,Saleem A,Shafman T,Emoto Y,Weichselbaum R,Kufe D

更新日期：1994-06-07 00:00:00

abstract：:Art forgeries have existed since antiquity, but with the recent rapidly expanding commercialization of art, the approach to art authentication has demanded increasingly sophisticated detection schemes. So far, the most conclusive criterion in the field of counterfeit detection is the scientific proof of material anach...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hendriks L,Hajdas I,Ferreira ESB,Scherrer NC,Zumbühl S,Smith GD,Welte C,Wacker L,Synal HA,Günther D

更新日期：2019-07-02 00:00:00

abstract：:Stat3 protein has an important role in oncogenesis and is a promising anticancer target. Indirubin, the active component of a traditional Chinese herbal medicine, has been shown previously to inhibit cyclin-dependent kinases, resulting in cell cycle arrest. Here, we show that the indirubin derivatives E564, E728, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nam S,Buettner R,Turkson J,Kim D,Cheng JQ,Muehlbeyer S,Hippe F,Vatter S,Merz KH,Eisenbrand G,Jove R

更新日期：2005-04-26 00:00:00

abstract：:Sequence comparisons of genomes or expressed sequence tags (ESTs) from related organisms provide insight into functional conservation and diversification. We compare the sequences of ESTs from the male accessory gland of Drosophila simulans to their orthologs in its close relative Drosophila melanogaster, and demonstr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swanson WJ,Clark AG,Waldrip-Dail HM,Wolfner MF,Aquadro CF

更新日期：2001-06-19 00:00:00

abstract：:We have developed a homologous in vitro transcription system that requires (i) 2,3,7,8-tetrachlorodibenzo-p-dioxin (called TCDD or dioxin), (ii) the Ah receptor, and (iii) a dioxin-responsive enhancer for activity. Unfractionated nuclear extracts from mouse hepatoma cells contain an inhibitor and fail to direct transc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wen LP,Koeiman N,Whitlock JP Jr

更新日期：1990-11-01 00:00:00

abstract：:T helper (Th) lymphocytes can develop memory for the expression of particular cytokines, like IL-4 or IL-10, in that reexpression of those cytokines is independent of the original costimulatory signal IL-4 and depends only on T cell receptor stimulation. Here, we show that in the course of Th2 cell differentiation in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Löhning M,Richter A,Stamm T,Hu-Li J,Assenmacher M,Paul WE,Radbruch A

更新日期：2003-10-14 00:00:00

abstract：:Human pluripotent stem cells have the potential to provide comprehensive model systems for the earliest stages of human ontogenesis. To serve in this capacity, these cells must undergo a targeted, stepwise differentiation process that follows a normal developmental timeline. Here we demonstrate the ability of both hum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer JS,Shearer RL,Capowski EE,Wright LS,Wallace KA,McMillan EL,Zhang SC,Gamm DM

更新日期：2009-09-29 00:00:00

abstract：:Developing tissues contain motile populations of cells that can self-organize into spatially ordered tissues based on differences in their interfacial surface energies. However, it is unclear how self-organization by this mechanism remains robust when interfacial energies become heterogeneous in either time or space. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cerchiari AE,Garbe JC,Jee NY,Todhunter ME,Broaders KE,Peehl DM,Desai TA,LaBarge MA,Thomson M,Gartner ZJ

更新日期：2015-02-17 00:00:00

abstract：:Ca2+, pheromones, sweet taste compounds, and the main neurotransmitters glutamate and gamma-aminobutyric acid activate G protein-coupled receptors (GPCRs) that constitute the GPCR family 3. These receptors are dimers, and each subunit has a large extracellular domain called a Venus flytrap module (VFTM), where agonist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bessis AS,Rondard P,Gaven F,Brabet I,Triballeau N,Prezeau L,Acher F,Pin JP

更新日期：2002-08-20 00:00:00

abstract：:15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gonnella NC,Birdseye TR,Nee M,Roberts JD

更新日期：1982-08-01 00:00:00

abstract：:Here, we highlight the potential translational benefits of delivering FLASH radiotherapy using ultra-high dose rates (>100 Gy⋅s-1). Compared with conventional dose-rate (CONV; 0.07-0.1 Gy⋅s-1) modalities, we showed that FLASH did not cause radiation-induced deficits in learning and memory in mice. Moreover, 6 months a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Montay-Gruel P,Acharya MM,Petersson K,Alikhani L,Yakkala C,Allen BD,Ollivier J,Petit B,Jorge PG,Syage AR,Nguyen TA,Baddour AAD,Lu C,Singh P,Moeckli R,Bochud F,Germond JF,Froidevaux P,Bailat C,Bourhis J,Vozenin MC

更新日期：2019-05-28 00:00:00

abstract：:The Cdc6 protein is essential for the assembly of pre-replicative complexes (pre-RCs) at origins of DNA replication in the budding yeast Saccharomyces cerevisiae. This reaction is blocked in vivo by the cyclin-dependent kinase Cdc28p, together with its regulatory subunits, the B type cyclins that are present throughou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donovan S,Harwood J,Drury LS,Diffley JF

更新日期：1997-05-27 00:00:00

abstract：:Carbon nanotube (CNT) membranes were employed as the active element of a switchable transdermal drug delivery device that can facilitate more effective treatments of drug abuse and addiction. Due to the dramatically fast flow through CNT cores, high charge density, and small pore dimensions, highly efficient electroph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu J,Paudel KS,Strasinger C,Hammell D,Stinchcomb AL,Hinds BJ

更新日期：2010-06-29 00:00:00

abstract：:Protein ubiquitination plays an important role in ATP-dependent protein turnover, and it also may regulate other cellular events. Covalent attachment of ubiquitin to other proteins is catalyzed by three different enzymes, E1, E2, and E3. We have previously shown that protein ubiquitination can be regulated by phosphor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kong SK,Chock PB

更新日期：1994-11-22 00:00:00

abstract：:We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vieira P,de Waal-Malefyt R,Dang MN,Johnson KE,Kastelein R,Fiorentino DF,deVries JE,Roncarolo MG,Mosmann TR,Moore KW

更新日期：1991-02-15 00:00:00

abstract：:Differentiation of naive CD4(+) T cells into IFN-gamma-producing T helper 1 (T(H)1) cells is pivotal for protective immune responses against intracellular pathogens. T-bet, a recently discovered member of the T-box transcription factor family, has been reported to play a critical role in this process, promoting IFN-ga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lighvani AA,Frucht DM,Jankovic D,Yamane H,Aliberti J,Hissong BD,Nguyen BV,Gadina M,Sher A,Paul WE,O'Shea JJ

更新日期：2001-12-18 00:00:00

abstract：:This study quantifies and maps the water footprint (WF) of humanity at a high spatial resolution. It reports on consumptive use of rainwater (green WF) and ground and surface water (blue WF) and volumes of water polluted (gray WF). Water footprints are estimated per nation from both a production and consumption perspe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoekstra AY,Mekonnen MM

更新日期：2012-02-28 00:00:00

abstract：:Fusions with the bacterial gene for chloramphenicol acetyltransferase followed by P-element-mediated germ-line transformation in Drosophila have permitted localization of the DNA sequence that confers a high degree of developmental specificity on a pair of silkmoth eggshell (chorion) genes. The short, 272-base-pair, 5...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsialis SA,Spoerel N,Leviten M,Kafatos FC

更新日期：1987-11-01 00:00:00

abstract：:Antigen-specific activation of T lymphocytes, via stimulation of the T-cell antigen receptor (TCR) complex, is marked by a rapid and sustained increase in the concentration of cytoplasmic free Ca2+ ([Ca2+]i). It has been suggested that the second messenger inositol 1,4,5-trisphosphate (IP3) produced after TCR stimulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jayaraman T,Ondriasová E,Ondrias K,Harnick DJ,Marks AR

更新日期：1995-06-20 00:00:00