Abstract:
:A simple in vitro system that supports chromatin assembly was developed for Saccharomyces cerevisiae. The assembly reaction is ATP-dependent, uses soluble histones and assembly factors, and generates physiologically spaced nucleosomes. We analyze the pathway of histone recruitment into nucleosomes, using this system in combination with genetic methods for the manipulation of yeast. This analysis supports the model of sequential recruitment of H3/H4 tetramers and H2A/H2B dimers into nucleosomes. Using a similar approach, we show that DNA ligase I can play an important role in template repair during assembly. These studies demonstrate the utility of this system for the combined biochemical and genetic analysis of chromatin assembly in yeast.
journal_name
Proc Natl Acad Sci U S Aauthors
Schultz MC,Hockman DJ,Harkness TA,Garinther WI,Altheim BAdoi
10.1073/pnas.94.17.9034subject
Has Abstractpub_date
1997-08-19 00:00:00pages
9034-9issue
17eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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