Crystal structure of measles virus hemagglutinin provides insight into effective vaccines.

Abstract:

:Measles still remains a major cause of childhood morbidity and mortality worldwide. Measles virus (MV) vaccines are highly successful, but the mechanism underlying their efficacy has been unclear. Here we report the crystal structure of the MV attachment protein, hemagglutinin, responsible for MV entry. The receptor-binding head domain exhibits a cubic-shaped beta-propeller structure and forms a homodimer. N-linked sugars appear to mask the broad regions and cause the two molecules forming the dimer to tilt oppositely toward the horizontal plane. Accordingly, residues of the putative receptor-binding site, highly conserved among MV strains, are strategically positioned in the unshielded area of the protein. These conserved residues also serve as epitopes for neutralizing antibodies, ensuring the serological monotype, a basis for effective MV vaccines. Our findings suggest that sugar moieties in the MV hemagglutinin critically modulate virus-receptor interaction as well as antiviral antibody responses, differently from sugars of the HIV gp120, which allow for immune evasion.

authors

Hashiguchi T,Kajikawa M,Maita N,Takeda M,Kuroki K,Sasaki K,Kohda D,Yanagi Y,Maenaka K

doi

10.1073/pnas.0707830104

subject

Has Abstract

pub_date

2007-12-04 00:00:00

pages

19535-40

issue

49

eissn

0027-8424

issn

1091-6490

pii

0707830104

journal_volume

104

pub_type

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