Toward an objective classification of cells in the immune system.

abstract：:The objective of this study was to determine whether the vascular smooth muscle contractile effect of NG-methyl-L-arginine (NMA) is endothelium dependent and attributed to a decline in smooth muscle levels of cyclic GMP. Vascular smooth muscle levels of cyclic GMP are severalfold greater in endothelium-intact than in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gold ME,Wood KS,Byrns RE,Fukuto J,Ignarro LJ

更新日期：1990-06-01 00:00:00

abstract：:Cell mechanics plays a role in stem cell reprogramming and differentiation. To understand this process better, we created a genetically encoded optical probe, named actin-cpstFRET-actin (AcpA), to report forces in actin in living cells in real time. We showed that stemness was associated with increased force in actin....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo J,Wang Y,Sachs F,Meng F

更新日期：2014-12-09 00:00:00

abstract：:Our long-term goal is the in vivo expression of intrinsically colored proteins without the need for further posttranslational modification or chemical functionalization by externally added reagents. Biocompatible (Aza)Indoles (Inds)/(Aza)Tryptophans (Trp) as optical probes represent almost ideal isosteric substitutes ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lepthien S,Hoesl MG,Merkel L,Budisa N

更新日期：2008-10-21 00:00:00

abstract：:The plant hormone indole-acetic acid (auxin) is essential for many aspects of plant development. Auxin-mediated growth regulation typically involves the establishment of an auxin concentration gradient mediated by polarly localized auxin transporters. The localization of auxin carriers and their amount at the plasma m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boutté Y,Jonsson K,McFarlane HE,Johnson E,Gendre D,Swarup R,Friml J,Samuels L,Robert S,Bhalerao RP

更新日期：2013-10-01 00:00:00

abstract：:Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in the gene encoding MeCP2. By binding to methylated CpG dinucleotide promoter regions, MeCP2 acts as a transcriptional repressor, predicting that its absence might result in widespread aberrant gene transcription, leading to the RTT phenotype. C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matarazzo V,Ronnett GV

更新日期：2004-05-18 00:00:00

abstract：:Multiple resistance and pH adaptation (Mrp) complexes are sophisticated cation/proton exchangers found in a vast variety of alkaliphilic and/or halophilic microorganisms, and are critical for their survival in highly challenging environments. This family of antiporters is likely to represent the ancestor of cation pum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li B,Zhang K,Nie Y,Wang X,Zhao Y,Zhang XC,Wu XL

更新日期：2020-12-08 00:00:00

abstract：:Smad proteins are key intracellular signaling effectors for the transforming growth factor-beta superfamily of peptide growth factors. Following receptor-induced activation, Smads move into the nucleus to activate transcription of a select set of target genes. The activity of Smad proteins must be tightly regulated to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Chang C,Gehling DJ,Hemmati-Brivanlou A,Derynck R

更新日期：2001-01-30 00:00:00

abstract：:Utrophin levels have recently been shown to be more abundant in slow vs. fast muscles, but the nature of the molecular events underlying this difference remains to be fully elucidated. Here, we determined whether this difference is due to the expression of utrophin A or B, and examined whether transcriptional regulato...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chakkalakal JV,Stocksley MA,Harrison MA,Angus LM,Deschenes-Furry J,St-Pierre S,Megeney LA,Chin ER,Michel RN,Jasmin BJ

更新日期：2003-06-24 00:00:00

abstract：:Many groups of insects are specialists in exploiting sensory cues to locate food resources or conspecifics. To achieve orientation, bees and ants analyze the polarization pattern of the sky, male moths orient along the females' odor plume, and cicadas, grasshoppers, and crickets use acoustic signals to locate singing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poulet JF,Hedwig B

更新日期：2005-10-25 00:00:00

abstract：:The phosphatidylinositol 3-kinase subunit PIK3CA is frequently mutated in human cancers. Here we used gene targeting to "knock in" PIK3CA mutations into human breast epithelial cells to identify new therapeutic targets associated with oncogenic PIK3CA. Mutant PIK3CA knockin cells were capable of epidermal growth facto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gustin JP,Karakas B,Weiss MB,Abukhdeir AM,Lauring J,Garay JP,Cosgrove D,Tamaki A,Konishi H,Konishi Y,Mohseni M,Wang G,Rosen DM,Denmeade SR,Higgins MJ,Vitolo MI,Bachman KE,Park BH

更新日期：2009-02-24 00:00:00

abstract：:The development of distinct regions in the amniote vertebral column results from somite formation and Hox gene expression, with the adult morphology displaying remarkable variation among lineages. Mammalian regionalization is reportedly very conservative or even constrained, but there has been no study investigating v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller J,Scheyer TM,Head JJ,Barrett PM,Werneburg I,Ericson PG,Pol D,Sánchez-Villagra MR

更新日期：2010-02-02 00:00:00

abstract：:Studies in mice indicate that the gut microbiota promotes energy harvest and storage from components of the diet when these components are plentiful. Here we examine how the microbiota shapes host metabolic and physiologic adaptations to periods of nutrient deprivation. Germ-free (GF) mice and mice who had received a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crawford PA,Crowley JR,Sambandam N,Muegge BD,Costello EK,Hamady M,Knight R,Gordon JI

更新日期：2009-07-07 00:00:00

abstract：:The abundances of (92)Nb and (146)Sm in the early solar system are determined from meteoritic analysis, and their stellar production is attributed to the p process. We investigate if their origin from thermonuclear supernovae deriving from the explosion of white dwarfs with mass above the Chandrasekhar limit is in agr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lugaro M,Pignatari M,Ott U,Zuber K,Travaglio C,Gyürky G,Fülöp Z

更新日期：2016-01-26 00:00:00

abstract：:Heme oxygenase (HO) catalyzes the opening of the heme ring with the release of iron in both plants and animals. In cyanobacteria, red algae, and cryptophyceae, HO is a key enzyme in the synthesis of the chromophoric part of the photosynthetic antennae. In an attempt to study the regulation of this key metabolic step, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richaud C,Zabulon G

更新日期：1997-10-14 00:00:00

abstract：:The tissue-specific expression of the chicken liver cell adhesion molecule (L-CAM) was studied by generating transgenic mice. The rat insulin II promoter was fused to a chicken L-CAM cDNA or to chicken genomic L-CAM sequences. Mice carrying the cDNA showed no expression of L-CAM. Mice carrying L-CAM genomic sequences ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Begemann M,Tan SS,Cunningham BA,Edelman GM

更新日期：1990-11-01 00:00:00

abstract：:We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNF1) and 87,093 (pNF2) bp with average G+C ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishikawa J,Yamashita A,Mikami Y,Hoshino Y,Kurita H,Hotta K,Shiba T,Hattori M

更新日期：2004-10-12 00:00:00

abstract：:The steady-state level and synthesis of a pair of polypeptides of Mr 33,000 and 35,000 in chicken embryo fibroblasts (CEF) transformed by Rous sarcoma virus (RSV) are significantly decreased relative to normal CEF; however, the decrease is more pronounced in the case of the Mr 35,000 polypeptide. These polypeptides ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hendricks M,Weintraub H

更新日期：1981-09-01 00:00:00

abstract：:The vacuolated lens (vl) mouse mutant causes congenital cataracts and neural tube defects (NTDs), with the NTDs being caused by abnormal neural fold apposition and fusion. Our positional cloning of vl indicates these phenotypes result from a deletion mutation in an uncharacterized orphan G protein-coupled receptor (GP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matteson PG,Desai J,Korstanje R,Lazar G,Borsuk TE,Rollins J,Kadambi S,Joseph J,Rahman T,Wink J,Benayed R,Paigen B,Millonig JH

更新日期：2008-02-12 00:00:00

abstract：:The B cell developmental pathway represents a leading system for the analysis of regulatory circuits that orchestrate cell fate specification and commitment. Considerable progress has been achieved within the past decade in the identification and genetic analysis of various regulatory components. These components incl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh H,Medina KL,Pongubala JM

更新日期：2005-04-05 00:00:00

abstract：:Evolutionary processes leading to adaptive radiation regularly occur too fast to be accurately recorded in the fossil record but too slowly to be readily observed in living biota. The study of evolutionary radiations is thereby confronted with an epistemological gap between the timescales and approaches used by neonto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Bocxlaer B,Hunt G

更新日期：2013-08-20 00:00:00

abstract：:Using data from the Fragile Families and Child Wellbeing Study, this study examined the effects of the Great Recession on maternal harsh parenting. We found that changes in macroeconomic conditions, rather than current conditions, affected harsh parenting, that declines in macroeconomic conditions had a stronger impac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee D,Brooks-Gunn J,McLanahan SS,Notterman D,Garfinkel I

更新日期：2013-08-20 00:00:00

abstract：:A comprehensive mapping of interactions among Epstein-Barr virus (EBV) proteins and interactions of EBV proteins with human proteins should provide specific hypotheses and a broad perspective on EBV strategies for replication and persistence. Interactions of EBV proteins with each other and with human proteins were as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calderwood MA,Venkatesan K,Xing L,Chase MR,Vazquez A,Holthaus AM,Ewence AE,Li N,Hirozane-Kishikawa T,Hill DE,Vidal M,Kieff E,Johannsen E

更新日期：2007-05-01 00:00:00

abstract：:Increased accumulation of lipofuscin in cells of the retinal pigment epithelium (RPE) is seen in several forms of macular degeneration, a common cause of blindness in humans. A major fluorophore of lipofuscin is the toxic bis-retinoid, N-retinylidene-N-retinylethanolamine (A2E). Previously, we generated mice with a kn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mata NL,Weng J,Travis GH

更新日期：2000-06-20 00:00:00

abstract：:Mitochondrial dysfunction is implicated in the pathophysiology of Parkinson's disease (PD), a common age-associated neurodegenerative disease characterized by intraneuronal inclusions (Lewy bodies) and progressive degeneration of the nigrostriatal dopamine (DA) system. It has recently been demonstrated that midbrain D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ekstrand MI,Terzioglu M,Galter D,Zhu S,Hofstetter C,Lindqvist E,Thams S,Bergstrand A,Hansson FS,Trifunovic A,Hoffer B,Cullheim S,Mohammed AH,Olson L,Larsson NG

更新日期：2007-01-23 00:00:00

abstract：:In an unprecedented finding, Davis et al. [Davis, R. E., Miller, S., Herrnstadt, C., Ghosh, S. S., Fahy, E., Shinobu, L. A., Galasko, D., Thal, L. J., Beal, M. F., Howell, N. & Parker, W. D., Jr. (1997) Proc. Natl. Acad. Sci. USA 94, 4526-4531] used an unusual DNA isolation method to show that healthy adults harbor a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirano M,Shtilbans A,Mayeux R,Davidson MM,DiMauro S,Knowles JA,Schon EA

更新日期：1997-12-23 00:00:00

abstract：:Secretion of inflammatory mediators by rat mast cells (line RBL-2H3) was earlier shown to be inhibited upon clustering a membrane glycoprotein by monoclonal antibody G63. This glycoprotein, named mast cell function-associated antigen (MAFA), was also shown to interfere with the coupling cascade of the type 1 Fc epsilo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guthmann MD,Tal M,Pecht I

更新日期：1995-09-26 00:00:00

abstract：:Stearoyl-acyl-carrier-protein-desaturase-mediated conversion of stearic acid (18:0) to oleic acid (18:1) is a key step, which regulates levels of unsaturated fatty acids in cells. We previously showed that stearoyl-acyl-carrier-protein-desaturase mutants ssi2/fab2 carrying a loss-of-function mutation in the plastidial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kachroo A,Venugopal SC,Lapchyk L,Falcone D,Hildebrand D,Kachroo P

更新日期：2004-04-06 00:00:00

abstract：:Chemokines and their receptors are key factors in the onset and progression of AIDS. Among them, accumulating evidence strongly indicates the involvement of IL-8 and its receptors, CXCR1 and CXCR2, in AIDS-related conditions. Through extensive investigation of genetic variations of the human CXCR1-CXCR2 locus, we iden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vasilescu A,Terashima Y,Enomoto M,Heath S,Poonpiriya V,Gatanaga H,Do H,Diop G,Hirtzig T,Auewarakul P,Lauhakirti D,Sura T,Charneau P,Marullo S,Therwath A,Oka S,Kanegasaki S,Lathrop M,Matsushima K,Zagury JF,Matsuda

更新日期：2007-02-27 00:00:00

abstract：:siRNA therapeutics have promise for the treatment of a wide range of genetic disorders. Motivated by lipoproteins, we report lipopeptide nanoparticles as potent and selective siRNA carriers with a wide therapeutic index. Lead material cKK-E12 showed potent silencing effects in mice (ED50 ∼ 0.002 mg/kg), rats (ED50 < 0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong Y,Love KT,Dorkin JR,Sirirungruang S,Zhang Y,Chen D,Bogorad RL,Yin H,Chen Y,Vegas AJ,Alabi CA,Sahay G,Olejnik KT,Wang W,Schroeder A,Lytton-Jean AK,Siegwart DJ,Akinc A,Barnes C,Barros SA,Carioto M,Fitzgerald

更新日期：2014-03-18 00:00:00

abstract：:Pancreatic beta cells respond to changes in blood glucose by secreting insulin and increasing insulin synthesis. To identify genes used in these responses, we have carried out expression profiling of beta cells exposed to high (25 mM) or low (5.5 mM) glucose by using oligonucleotide microarrays. Functional clustering ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Webb GC,Akbar MS,Zhao C,Steiner DF

更新日期：2000-05-23 00:00:00