Abstract:
:Multiple resistance and pH adaptation (Mrp) complexes are sophisticated cation/proton exchangers found in a vast variety of alkaliphilic and/or halophilic microorganisms, and are critical for their survival in highly challenging environments. This family of antiporters is likely to represent the ancestor of cation pumps found in many redox-driven transporter complexes, including the complex I of the respiratory chain. Here, we present the three-dimensional structure of the Mrp complex from a Dietzia sp. strain solved at 3.0-Å resolution using the single-particle cryoelectron microscopy method. Our structure-based mutagenesis and functional analyses suggest that the substrate translocation pathways for the driving substance protons and the substrate sodium ions are separated in two modules and that symmetry-restrained conformational change underlies the functional cycle of the transporter. Our findings shed light on mechanisms of redox-driven primary active transporters, and explain how driving substances of different electric charges may drive similar transport processes.
journal_name
Proc Natl Acad Sci U S Aauthors
Li B,Zhang K,Nie Y,Wang X,Zhao Y,Zhang XC,Wu XLdoi
10.1073/pnas.2006276117subject
Has Abstractpub_date
2020-12-08 00:00:00pages
31166-31176issue
49eissn
0027-8424issn
1091-6490pii
2006276117journal_volume
117pub_type
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