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Regulation of toxin synthesis in Clostridium difficile by an alternative RNA polymerase sigma factor.

Abstract：

:Clostridium difficile, a causative agent of antibiotic-associated diarrhea and its potentially lethal form, pseudomembranous colitis, produces two large protein toxins that are responsible for the cellular damage associated with the disease. The level of toxin production appears to be critical for determining the severity of the disease, but the mechanism by which toxin synthesis is regulated is unknown. The product of a gene, txeR, that lies just upstream of the tox gene cluster was shown to be needed for tox gene expression in vivo and to activate promoter-specific transcription of the tox genes in vitro in conjunction with RNA polymerases from C. difficile, Bacillus subtilis, or Escherichia coli. TxeR was shown to function as an alternative sigma factor for RNA polymerase. Because homologs of TxeR regulate synthesis of toxins and a bacteriocin in other Clostridium species, TxeR appears to be a prototype for a novel mode of regulation of toxin genes.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Mani N,Dupuy B

doi

10.1073/pnas.101126598

keywords:

subject

Has Abstract

pub_date

2001-05-08 00:00:00

pages

5844-9

issue

10

eissn

0027-8424

issn

1091-6490

pii

101126598

journal_volume

98

pub_type

杂志文章

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