Cellular heterogeneity profiling by hyaluronan probes reveals an invasive but slow-growing breast tumor subset.

abstract：:Mach bands, the illusory brightness maxima and minima perceived at the initiation and termination of luminance gradients, respectively, are generally considered a direct perceptual manifestation of lateral inhibitory interactions among retinal or other lower order visual neurons. Here we examine an alternative explana...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lotto RB,Williams SM,Purves D

更新日期：1999-04-27 00:00:00

abstract：:Treatment of myoblast cell lines with 3-deazaadenosine stimulates differentiation into myofibers. Myoblast clone L5/ 3B5 , which does not form myofibers after 6 days in fusion medium, was stimulated to form myofibers after 5 days of culture in fusion medium containing 50 microM 3-deazaadenosine. Myoblast clone L5/ 3C4...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scarpa S,Strom R,Bozzi A,Aksamit RR,Backlund PS Jr,Chen J,Cantoni GL

更新日期：1984-05-01 00:00:00

abstract：:In the central nervous system, myelin proteolipid protein isoforms (PLP and DM20) play an essential structural role in myelination. It has been shown in several species that myelination is impaired by molecular defects resulting from single base mutations in the PLP gene. We have used DNA amplification by polymerase c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pham-Dinh D,Popot JL,Boespflug-Tanguy O,Landrieu P,Deleuze JF,Boué J,Jollès P,Dautigny A

更新日期：1991-09-01 00:00:00

abstract：:The three-dimensional organization of HLA antigens has been investigated by spectroscopic and immunochemical techniques. Measurement of the circular dichroism shows that in papain-solubilized HLA the heavy chain as well as the previously studied light chain (beta 2-microglobulin) consists predominantly of beta-pleated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lancet D,Parham P,Strominger JL

更新日期：1979-08-01 00:00:00

abstract：:Epithelia in animals are colonized by complex communities of microbes. Although a topic of long-standing interest, understanding the evolution of the microbial communities and their role in triggering innate immune responses has resisted analysis. Cnidaria are among the simplest animals at the tissue grade of organiza...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fraune S,Bosch TC

更新日期：2007-08-07 00:00:00

abstract：:The placenta is essential for the success of therian mammalian reproduction. Intense selective pressure has shaped changes in placental anatomy and function during mammalian cladogenesis. Here we challenge the view that the hemochorial placenta is a derived feature in haplorhine primates. Using phylogenetic and statis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wildman DE,Chen C,Erez O,Grossman LI,Goodman M,Romero R

更新日期：2006-02-28 00:00:00

abstract：:Cedar virus (CedV) is a bat-borne henipavirus related to Nipah virus (NiV) and Hendra virus (HeV), zoonotic agents of fatal human disease. CedV receptor-binding protein (G) shares only ∼30% sequence identity with those of NiV and HeV, although they can all use ephrin-B2 as an entry receptor. We demonstrate that CedV a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laing ED,Navaratnarajah CK,Cheliout Da Silva S,Petzing SR,Xu Y,Sterling SL,Marsh GA,Wang LF,Amaya M,Nikolov DB,Cattaneo R,Broder CC,Xu K

更新日期：2019-10-08 00:00:00

abstract：:A strain of Escherichia coli, selected on the basis of its resistance to colicin N, reveals distinct structural and functional alterations in unspecific OmpF porin. A single mutation [Gly-119-->Asp (G119D)] was identified in the internal loop L3 that contributes critically to the formation of the construction inside t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jeanteur D,Schirmer T,Fourel D,Simonet V,Rummel G,Widmer C,Rosenbusch JP,Pattus F,Pagès JM

更新日期：1994-10-25 00:00:00

abstract：:Cytoplasmic membrane vesicles prepared from Escherichia coli containing multiple copies of the lac y gene were frozen in liquid nitrogen before or after generation of a proton electrochemical gradient (interior negative and alkaline) and irradiated with a high-energy electron beam at -135 degrees C. Subsequently, the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldkorn T,Rimon G,Kempner ES,Kaback HR

更新日期：1984-02-01 00:00:00

abstract：:To initiate class switch recombination (CSR) activation-induced cytidine deaminase (AID) induces staggered nick cleavage in the S region, which lies 5' to each Ig constant region gene and is rich in palindromic sequences. Topoisomerase 1 (Top1) controls the supercoiling of DNA by nicking, rotating, and religating one ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kobayashi M,Aida M,Nagaoka H,Begum NA,Kitawaki Y,Nakata M,Stanlie A,Doi T,Kato L,Okazaki IM,Shinkura R,Muramatsu M,Kinoshita K,Honjo T

更新日期：2009-12-29 00:00:00

abstract：:The role of the renin-angiotensin system in blood pressure control and in the development of hypertension was investigated by generating transgenic mice carrying the rat renin or angiotensinogen gene or both genes under the control of the mouse metallothionein I promoter. The systolic blood pressure was significantly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohkubo H,Kawakami H,Kakehi Y,Takumi T,Arai H,Yokota Y,Iwai M,Tanabe Y,Masu M,Hata J

更新日期：1990-07-01 00:00:00

abstract：:Chemoresistance of cisplatin therapy is related to extensive repair of cisplatin-modified DNA in the nucleus by the nucleotide excision repair (NER). Delivering cisplatin to the mitochondria to attack mitochondrial genome lacking NER machinery can lead to a rationally designed therapy for metastatic, chemoresistant ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marrache S,Pathak RK,Dhar S

更新日期：2014-07-22 00:00:00

abstract：:In choanoflagellates, the closest living relatives of animals, multicellular rosette development is regulated by environmental bacteria. The simplicity of this evolutionarily relevant interaction provides an opportunity to identify the molecules and regulatory logic underpinning bacterial regulation of development. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woznica A,Cantley AM,Beemelmanns C,Freinkman E,Clardy J,King N

更新日期：2016-07-12 00:00:00

abstract：:We provide evidence for an interaction between mouse class I major histocompatibility complex antigens and insulin receptors. Antibodies against class I but not class II major histocompatibility complex antigens immunoprecipitate photoaffinity-labeled hepatic insulin receptors. Haplotype specificity is demonstrated by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillips ML,Moule ML,Delovitch TL,Yip CC

更新日期：1986-05-01 00:00:00

abstract：:Recently, hepatic peroxisome proliferator-activated receptor (PPAR)γ has been implicated in hepatic lipid accumulation. We found that the C3H mouse strain does not express PPARγ in the liver and, when subject to a high-fat diet, is resistant to hepatic steatosis, compared with C57BL/6 (B6) mice. Adenoviral PPARγ2 inje...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee YJ,Ko EH,Kim JE,Kim E,Lee H,Choi H,Yu JH,Kim HJ,Seong JK,Kim KS,Kim JW

更新日期：2012-08-21 00:00:00

abstract：:Aerosols impact climate, human health, and the chemistry of the atmosphere, and aerosol pH plays a major role in the physicochemical properties of the aerosol. However, there remains uncertainty as to whether aerosols are acidic, neutral, or basic. In this research, we show that the pH of freshly emitted (nascent) sea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Angle KJ,Crocker DR,Simpson RMC,Mayer KJ,Garofalo LA,Moore AN,Mora Garcia SL,Or VW,Srinivasan S,Farhan M,Sauer JS,Lee C,Pothier MA,Farmer DK,Martz TR,Bertram TH,Cappa CD,Prather KA,Grassian VH

更新日期：2021-01-12 00:00:00

abstract：:Noncanonical DNA structures that stall DNA replication can cause errors in genomic DNA. Here, we investigated how the noncanonical structures formed by sequences in genes associated with a number of diseases impacted DNA polymerization by the Klenow fragment of DNA polymerase. Replication of a DNA sequence forming an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takahashi S,Brazier JA,Sugimoto N

更新日期：2017-09-05 00:00:00

abstract：:A gene's expression pattern provides clues to its role in normal physiology and disease. To provide quantitative expression levels on a genome-wide scale, the Cancer Genome Anatomy Project (CGAP) uses serial analysis of gene expression (SAGE). Over 5 million transcript tags from more than 100 human cell types have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boon K,Osorio EC,Greenhut SF,Schaefer CF,Shoemaker J,Polyak K,Morin PJ,Buetow KH,Strausberg RL,De Souza SJ,Riggins GJ

更新日期：2002-08-20 00:00:00

abstract：:The 32-kDa subunit of replication protein A (RPA) is phosphorylated during the S phase of the cell cycle in vivo and during simian virus 40 DNA replication in vitro. To explore the functional significance of this modification, we purified a HeLa cell protein kinase that phosphorylates RPA in the presence of single-str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brush GS,Anderson CW,Kelly TJ

更新日期：1994-12-20 00:00:00

abstract：:Phosphatidylionsitol 4,5-bisphosphate (PIP(2)), a substrate of phospholipase C, has recently been recognized to regulate membrane-associated proteins and act as a signal molecule in phospholipase C-linked Gq-coupled receptor (GqPCR) pathways. However, it is not known whether PIP(2) depletion induced by GqPCRs can act ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho H,Lee D,Lee SH,Ho WK

更新日期：2005-03-22 00:00:00

abstract：:Cellular and molecular signals involved in axon elongation versus collateral and arbor formation may be intrinsic to developing neurons, or they may derive from targets. To identify signals regulating axon growth modes, we have developed a culture system in which trigeminal ganglion cells are challenged by various tar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Erzurumlu RS,Jhaveri S,Takahashi H,McKay RD

更新日期：1993-08-01 00:00:00

abstract：:PUF proteins control both growth and differentiation in the C. elegans germ line. These conserved RNA-binding proteins inhibit expression of target mRNAs, either by repressing translation or promoting degradation. Previous studies showed that PUF-8, a PUF protein with striking similarity to human Pumilio, prevents ret...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bachorik JL,Kimble J

更新日期：2005-08-02 00:00:00

abstract：:Infectious diseases of domesticated animals impact human well-being via food insecurity, loss of livelihoods, and human infections. While much research has focused on parasites that infect single host species, most parasites of domesticated mammals infect multiple species. The impact of multihost parasites varies acro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Farrell MJ,Davies TJ

更新日期：2019-04-16 00:00:00

abstract：:The het-s locus of Podospora anserina is a heterokaryon incompatibility locus. The coexpression of the antagonistic het-s and het-S alleles triggers a lethal reaction that prevents the formation of viable heterokaryons. Strains that contain the het-s allele can display two different phenotypes, [Het-s] or [Het-s*], ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coustou V,Deleu C,Saupe S,Begueret J

更新日期：1997-09-02 00:00:00

abstract：:Rhizobia colonize their legume hosts by different modes of entry while initiating symbiotic nitrogen fixation. Most legumes are invaded via growing root hairs by the root hair-curl mechanism, which involves epidermal cell responses. However, invasion of a number of tropical legumes happens through fissures at lateral ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goormachtig S,Capoen W,James EK,Holsters M

更新日期：2004-04-20 00:00:00

abstract：:Microinjection of plasmids encoding human tau (htau) protein into identified lamprey reticulospinal neurons (anterior bulbar cells, or ABCs) in situ induces chronic htau expression. htau protein is transported to both the axon and dendrites of expressing ABCs by mechanisms that require the C-terminal domain of htau pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall GF,Yao J,Lee G

更新日期：1997-04-29 00:00:00

abstract：:We propose that protein translocation across membranes is driven by biased random thermal motion. This "Brownian ratchet" mechanism depends on chemical asymmetries between the cis and trans sides of the membrane. Several mechanisms could contribute to rectifying the thermal motion of the protein, such as binding and d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simon SM,Peskin CS,Oster GF

更新日期：1992-05-01 00:00:00

abstract：:Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armache JP,Jarasch A,Anger AM,Villa E,Becker T,Bhushan S,Jossinet F,Habeck M,Dindar G,Franckenberg S,Marquez V,Mielke T,Thomm M,Berninghausen O,Beatrix B,Söding J,Westhof E,Wilson DN,Beckmann R

更新日期：2010-11-16 00:00:00

abstract：:Gene expression is a stochastic, or "noisy," process. This noise comes about in two ways. The inherent stochasticity of biochemical processes such as transcription and translation generates "intrinsic" noise. In addition, fluctuations in the amounts or states of other cellular components lead indirectly to variation i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swain PS,Elowitz MB,Siggia ED

更新日期：2002-10-01 00:00:00

abstract：:The method of in situ hybridization has become a significant technique for specific-site chromosome mapping. We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zabel BU,Naylor SL,Sakaguchi AY,Bell GI,Shows TB

更新日期：1983-11-01 00:00:00