Target-derived influences on axon growth modes in cultures of trigeminal neurons.

Abstract:

:Cellular and molecular signals involved in axon elongation versus collateral and arbor formation may be intrinsic to developing neurons, or they may derive from targets. To identify signals regulating axon growth modes, we have developed a culture system in which trigeminal ganglion cells are challenged by various target tissues. Embryonic day 15 (E15) rat trigeminal ganglion explants were placed between peripheral (vibrissa pad) and central nervous system targets. Normally, bipolar trigeminal ganglion cells extend one process to the vibrissa pad and another to the brainstem trigeminal complex. Under coculture conditions, the peripheral processes invade the vibrissa pad explants and form a characteristic circumfollicular pattern. Central processes of E15 ganglion cells invade many, but not all, central nervous system tissues. In isochronic (E15) central nervous system explants such as brainstem, olfactory bulb, or neocortex, these central processes elongate and form a "tract" but have virtually no arbors. However, in more mature targets (e.g., a section from postnatal brainstem or neocortex), they form arbors instead of a tract. We conclude from these observations that whether trigeminal axons elongate to form a tract, or whether they begin to arborize, is dictated by the target tissue and not by an intrinsic developmental program of the ganglion cell body. The explant coculture system is an excellent model for analysis of the molecular basis of neuron-target interactions.

authors

Erzurumlu RS,Jhaveri S,Takahashi H,McKay RD

doi

10.1073/pnas.90.15.7235

subject

Has Abstract

pub_date

1993-08-01 00:00:00

pages

7235-9

issue

15

eissn

0027-8424

issn

1091-6490

journal_volume

90

pub_type

杂志文章
  • The neural development and organization of letter recognition: evidence from functional neuroimaging, computational modeling, and behavioral studies.

    abstract::Although much of the brain's functional organization is genetically predetermined, it appears that some noninnate functions can come to depend on dedicated and segregated neural tissue. In this paper, we describe a series of experiments that have investigated the neural development and organization of one such noninna...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章,评审

    doi:10.1073/pnas.95.3.847

    authors: Polk TA,Farah MJ

    更新日期:1998-02-03 00:00:00

  • Metachromatic leukodystrophy: arylsulfatase-A deficiency in skin fibroblast cultures.

    abstract::Fibroblasts cultured from the skin of a patient with metachromatic leukodystrophy have been found to manifest the biochemical defect of this inborn error of metabolism, a deficiency of arylsulfatase A. Diseased cells had less than five per cent of normal arylsulfatase-A activity, while activities of other lysosomal en...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.62.3.887

    authors: Porter MT,Fluharty AL,Kihara H

    更新日期:1969-03-01 00:00:00

  • Ten- to 50-nm-long quasi-ballistic carbon nanotube devices obtained without complex lithography.

    abstract::A simple method combining photolithography and shadow (or angle) evaporation is developed to fabricate single-walled carbon nanotube (SWCNT) devices with tube lengths of approximately 10-50 nm between metal contacts. Large numbers of such short devices are obtained without the need of complex tools such as electron be...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404450101

    authors: Javey A,Qi P,Wang Q,Dai H

    更新日期:2004-09-14 00:00:00

  • The scaling of frontal cortex in primates and carnivores.

    abstract::Size has a profound effect on the structure of the brain. Many brain structures scale allometrically, that is, their relative size changes systematically as a function of brain size. Here we use independent contrasts analysis to examine the scaling of frontal cortex in 43 species of mammals including 25 primates and 1...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0305760101

    authors: Bush EC,Allman JM

    更新日期:2004-03-16 00:00:00

  • Population declines in North American birds that migrate to the neotropics.

    abstract::Using data from the North American Breeding Bird Survey, we determined that most neotropical migrant bird species that breed in forests of the eastern United States and Canada have recently (1978-1987) declined in abundance after a period of stable or increasing populations. Most permanent residents and temperate-zone...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.19.7658

    authors: Robbins CS,Sauer JR,Greenberg RS,Droege S

    更新日期:1989-10-01 00:00:00

  • Deep learning enables high-quality and high-throughput prediction of enzyme commission numbers.

    abstract::High-quality and high-throughput prediction of enzyme commission (EC) numbers is essential for accurate understanding of enzyme functions, which have many implications in pathologies and industrial biotechnology. Several EC number prediction tools are currently available, but their prediction performance needs to be f...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1821905116

    authors: Ryu JY,Kim HU,Lee SY

    更新日期:2019-07-09 00:00:00

  • DNA methylation in the gene body influences MeCP2-mediated gene repression.

    abstract::Rett syndrome is a severe neurodevelopmental disorder caused by mutations in the methyl-CpG binding protein gene (MECP2). MeCP2 is a methyl-cytosine binding protein that is proposed to function as a transcriptional repressor. However, multiple gene expression studies comparing wild-type and MeCP2-deficient neurons hav...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1618737114

    authors: Kinde B,Wu DY,Greenberg ME,Gabel HW

    更新日期:2016-12-27 00:00:00

  • Integrated logic circuits using single-atom transistors.

    abstract::Scaling down the size of computing circuits is about to reach the limitations imposed by the discrete atomic structure of matter. Reducing the power requirements and thereby dissipation of integrated circuits is also essential. New paradigms are needed to sustain the rate of progress that society has become used to. S...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1109935108

    authors: Mol JA,Verduijn J,Levine RD,Remacle F,Rogge S

    更新日期:2011-08-23 00:00:00

  • Effects of the peer metagenomic environment on smoking behavior.

    abstract::Recent scholarship suggests that the genomes of those around us affect our own phenotypes. Much of the empirical evidence for such "metagenomic" effects comes from animal studies, where the socio-genetic environment can be easily manipulated. Among humans, it is more difficult to identify such effects given the nonran...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1806901116

    authors: Sotoudeh R,Harris KM,Conley D

    更新日期:2019-08-13 00:00:00

  • Mathematical model of renal interstitial fibrosis.

    abstract::Lupus nephritis (LN) is an autoimmune disease that occurs when autoantibodies complex with self-antigen and form immune complexes that accumulate in the glomeruli. These immune complexes initiate an inflammatory response resulting in glomerular injury. LN often concomitantly affects the tubulointerstitial compartment ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1413970111

    authors: Hao W,Rovin BH,Friedman A

    更新日期:2014-09-30 00:00:00

  • Gonadal and extragonadal expression of inhibin alpha, beta A, and beta B subunits in various tissues predicts diverse functions.

    abstract::The S1-nuclease analysis was used to investigate the pattern of inhibin expression in the rat. In a first series of experiments, expression of the alpha, beta A, and beta B subunits of inhibin were monitored in various tissues from male and female rats. Two observations emerge from these studies. First, expression of ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.1.247

    authors: Meunier H,Rivier C,Evans RM,Vale W

    更新日期:1988-01-01 00:00:00

  • Resistance to action potential depression of a rat axon terminal in vivo.

    abstract::The shape of the presynaptic action potential (AP) has a strong impact on neurotransmitter release. Because of the small size of most terminals in the central nervous system, little is known about the regulation of their AP shape during natural firing patterns in vivo. The calyx of Held is a giant axosomatic terminal ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1619433114

    authors: Sierksma MC,Borst JGG

    更新日期:2017-04-18 00:00:00

  • Cholecystokinin release triggered by NMDA receptors produces LTP and sound-sound associative memory.

    abstract::Memory is stored in neural networks via changes in synaptic strength mediated in part by NMDA receptor (NMDAR)-dependent long-term potentiation (LTP). Here we show that a cholecystokinin (CCK)-B receptor (CCKBR) antagonist blocks high-frequency stimulation-induced neocortical LTP, whereas local infusion of CCK induces...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1816833116

    authors: Chen X,Li X,Wong YT,Zheng X,Wang H,Peng Y,Feng H,Feng J,Baibado JT,Jesky R,Wang Z,Xie H,Sun W,Zhang Z,Zhang X,He L,Zhang N,Zhang Z,Tang P,Su J,Hu LL,Liu Q,He X,Tan A,Sun X,Li M,Wong K,Wang X,Cheung

    更新日期:2019-03-26 00:00:00

  • The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 Da: analysis of sequence differences between type I and type II keratins.

    abstract::We present the complete nucleotide and deduced amino acid sequences of a mouse epidermal keratin subunit of 60,000 Da. The keratin possesses a central alpha-helical domain of four tracts (termed 1A, 1B, 2A, and 2B) that can form coiled-coils, interspersed by short linker sequences, and has non-alpha-helical terminal d...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.18.5709

    authors: Steinert PM,Parry DA,Racoosin EL,Idler WW,Steven AC,Trus BL,Roop DR

    更新日期:1984-09-01 00:00:00

  • Neurotrophic activity of platelet-derived growth factor (PDGF): Rat neuronal cells possess functional PDGF beta-type receptors and respond to PDGF.

    abstract::The different isoforms of PDGF bind with different affinities to two different receptor types. Previously, these receptors have been demonstrated mainly on mesenchymal and glial cells. We show here that PDGF beta-type receptors are present also on rat brain neurons in vivo as well as in vitro. Immunohistochemical anal...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.18.8159

    authors: Smits A,Kato M,Westermark B,Nistér M,Heldin CH,Funa K

    更新日期:1991-09-15 00:00:00

  • A site-specific, multiplexed kinase activity assay using stable-isotope dilution and high-resolution mass spectrometry.

    abstract::Most kinases are capable of recognizing and phosphorylating peptides containing short, linear sequence motifs. To measure the activation state of many kinases from the same cell lysate, we created a multiplexed, mass-spectrometry-based in vitro kinase assay. Ninety chemically synthesized peptides derived from well-cha...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0905165106

    authors: Yu Y,Anjum R,Kubota K,Rush J,Villen J,Gygi SP

    更新日期:2009-07-14 00:00:00

  • Visualizing the dynamics of viral replication in living cells via Tat peptide delivery of nuclease-resistant molecular beacons.

    abstract::In this study, we describe the use of nuclease-resistant molecular beacons (MBs) for the real-time detection of coxsackievirus B6 replication in living Buffalo green monkey kidney (BGMK) cells via Tat peptide delivery. A nuclease-resistant MB containing 2'-O-methyl RNA bases with phosphorothioate internucleotide linka...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0807066105

    authors: Yeh HY,Yates MV,Mulchandani A,Chen W

    更新日期:2008-11-11 00:00:00

  • DNA methyltransferase 3B regulates duration of neural crest production via repression of Sox10.

    abstract::Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1318408111

    authors: Hu N,Strobl-Mazzulla PH,Simoes-Costa M,Sánchez-Vásquez E,Bronner ME

    更新日期:2014-12-16 00:00:00

  • Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets.

    abstract::Selection of aptamers from nucleic acid libraries by in vitro evolution represents a powerful method of identifying high-affinity ligands for a broad range of molecular targets. Nevertheless, a sizeable fraction of proteins remain difficult targets due to inherently limited chemical diversity of nucleic acids. We have...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1213933109

    authors: Davies DR,Gelinas AD,Zhang C,Rohloff JC,Carter JD,O'Connell D,Waugh SM,Wolk SK,Mayfield WS,Burgin AB,Edwards TE,Stewart LJ,Gold L,Janjic N,Jarvis TC

    更新日期:2012-12-04 00:00:00

  • Selective viral vector transduction of ErbB4 expressing cortical interneurons in vivo with a viral receptor-ligand bridge protein.

    abstract::Both treatment of disease and basic studies of complex tissues can benefit from directing viral vector infection to specific cell types. We have used a unique cell targeting method to direct viral vector transduction to cerebral cortical neurons expressing the neuregulin (NRG) receptor ErbB4; both NRG and ErbB4 have b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1006233107

    authors: Choi J,Young JA,Callaway EM

    更新日期:2010-09-21 00:00:00

  • Evolutionary alteration of ALOX15 specificity optimizes the biosynthesis of antiinflammatory and proresolving lipoxins.

    abstract::ALOX15 (12/15-lipoxygenase) orthologs have been implicated in maturational degradation of intracellular organelles and in the biosynthesis of antiinflammatory and proresolving eicosanoids. Here we hypothesized that lower mammals (mice, rats, pigs) express 12-lipoxygenating ALOX15 orthologs. In contrast, 15-lipoxygenat...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1604029113

    authors: Adel S,Karst F,González-Lafont À,Pekárová M,Saura P,Masgrau L,Lluch JM,Stehling S,Horn T,Kuhn H,Heydeck D

    更新日期:2016-07-26 00:00:00

  • Restriction endonuclease cleavage maps of animal mitochondrial DNAs.

    abstract::The restriction endonuclease, HindIII, gives rise to three fragments in each of the three mitochondrial DNAs isolated from the established mammalian cell lines LA9 (mouse), HeLa (human), and BSC-1 (African green monkey). The restriction endonuclease, EcoRI, gives rise to three fragments in mitochondrial DNA from HeLa ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.71.11.4617

    authors: Brown WM,Vinograd J

    更新日期:1974-11-01 00:00:00

  • Consequences of seafood mislabeling for marine populations and fisheries management.

    abstract::Over the past decade, seafood mislabeling has been increasingly documented, raising public concern over the identity, safety, and sustainability of seafood. Negative outcomes from seafood mislabeling are suspected to be substantial and pervasive as seafood is the world's most highly traded food commodity. Here we prov...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2003741117

    authors: Kroetz K,Luque GM,Gephart JA,Jardine SL,Lee P,Chicojay Moore K,Cole C,Steinkruger A,Donlan CJ

    更新日期:2020-12-01 00:00:00

  • Initiation of meiotic recombination is independent of interhomologue interactions.

    abstract::In yeast meiosis, crossing-over between homologues is dependent upon double-strand breaks. We demonstrate that the occurrence of these breaks is independent of pairing between homologues by showing that they occur with normal frequency, timing, and position in the absence of a homologue. This observation supports mode...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.25.11934

    authors: Gilbertson LA,Stahl FW

    更新日期:1994-12-06 00:00:00

  • Nuclear uptake of 1,25-dihydroxy[3H]cholecalciferol in dispersed fibroblasts cultured from normal human skin.

    abstract::Because of the relative inaccessibility of known calciferol target tissues (i.e., intestine and bone), we examined fibroblasts derived from normal human skin and grown in tissue culture as a means of evaluating the interaction of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] and its effector system. When dispersed, inta...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.4.2562

    authors: Eil C,Marx SJ

    更新日期:1981-04-01 00:00:00

  • Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.

    abstract::Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target m...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.242606799

    authors: Calin GA,Dumitru CD,Shimizu M,Bichi R,Zupo S,Noch E,Aldler H,Rattan S,Keating M,Rai K,Rassenti L,Kipps T,Negrini M,Bullrich F,Croce CM

    更新日期:2002-11-26 00:00:00

  • Estimation of continental Os/Os values by using Os/Os and Nd/Nd ratios in marine manganese nodules.

    abstract::The relationship between (187)Os/(186)Os and (143)Nd/(144)Nd in different manganese nodule fields is used to determine the (187)Os/(186)Os ratio of the continental terrains bounding the major ocean basins. The Atlantic Ocean drainages yield (187)Os/(186)Os of about 11; the Pacific Ocean, between 25 and 36; and the wes...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.24.8032

    authors: Turekian KK,Luck JM

    更新日期:1984-12-01 00:00:00

  • Transient Darwinian selection in Salmonella enterica serovar Paratyphi A during 450 years of global spread of enteric fever.

    abstract::Multiple epidemic diseases have been designated as emerging or reemerging because the numbers of clinical cases have increased. Emerging diseases are often suspected to be driven by increased virulence or fitness, possibly associated with the gain of novel genes or mutations. However, the time period over which humans...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1411012111

    authors: Zhou Z,McCann A,Weill FX,Blin C,Nair S,Wain J,Dougan G,Achtman M

    更新日期:2014-08-19 00:00:00

  • Tat protein of human immunodeficiency virus type 1 represses expression of manganese superoxide dismutase in HeLa cells.

    abstract::Using a HeLa cell line stably transfected with the tat gene from human immunodeficiency virus type 1, we have found that the expression of the regulatory Tat protein suppresses the expression of cellular Mn-containing superoxide dismutase (Mn-SOD). This enzyme is one of the cell's primary defenses against oxygen-deriv...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.16.7632

    authors: Flores SC,Marecki JC,Harper KP,Bose SK,Nelson SK,McCord JM

    更新日期:1993-08-15 00:00:00

  • Effect of rectangle length on velocity thresholds for real movement.

    abstract::New data are reported on the influence of size on the velocity threshold for real movement. As the length of a narrow stimulus object increases, width remaining constant, the velocity threshold also increases. These findings are discussed in relation to some psychophysical experiments on contrast and some physiologica...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.65.3.516

    authors: Mates B,Graham CH

    更新日期:1970-03-01 00:00:00