Reduced Mg2+ block of N-methyl-D-aspartate receptor-mediated synaptic potentials in developing visual cortex.

abstract：:The spatial distribution of plant defenses within a leaf may be critical in explaining patterns of herbivory. The generalist lepidopteran larvae, Helicoverpa armigera (the cotton bollworm), avoided the midvein and periphery of Arabidopsis thaliana rosette leaves and fed almost exclusively on the inner lamina. This fee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shroff R,Vergara F,Muck A,Svatos A,Gershenzon J

更新日期：2008-04-22 00:00:00

abstract：:A hallmark of rheumatoid arthritis is the formation of an aggressive, tumor-like structure called pannus that erodes the joint. A major cellular component of the pannus is the fibroblast-like synoviocyte (FLS), whose morphology strikingly resembles that of a transformed cell, but underlying mechanisms of this "transfo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Makarov SS

更新日期：2006-11-14 00:00:00

abstract：:The B-cell antigen receptor is composed of membrane immunoglobulin sheathed by an alpha/beta heterodimer. The complex is noncovalently associated with protein kinase activity, and crosslinking of the receptor leads to capping and transmembrane signaling. Here we show that the sheath is not necessary either for this ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams GT,Peaker CJ,Patel KJ,Neuberger MS

更新日期：1994-01-18 00:00:00

abstract：:Let X(0) be an unknown M by N matrix. In matrix recovery, one takes n < MN linear measurements y(1),…,y(n) of X(0), where y(i) = Tr(A(T)iX(0)) and each A(i) is an M by N matrix. A popular approach for matrix recovery is nuclear norm minimization (NNM): solving the convex optimization problem min ||X||*subject to y(i) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donoho DL,Gavish M,Montanari A

更新日期：2013-05-21 00:00:00

abstract：:We introduce a polynomial C(mu)[Z; q, t], depending on a set of variables Z = z(1), z(2),..., a partition mu, and two extra parameters q, t. The definition of C(mu) involves a pair of statistics (maj(sigma, mu), inv(sigma, mu)) on words sigma of positive integers, and the coefficients of the z(i) are manifestly in N[q...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haglund J

更新日期：2004-11-16 00:00:00

abstract：:The Hippo pathway is an evolutionarily conserved regulator of organ growth and tumorigenesis. In Drosophila, oncogenic RasV12 cooperates with loss-of-cell polarity to promote Hippo pathway-dependent tumor growth. To identify additional factors that modulate this signaling, we performed a genetic screen utilizing the D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo X,Sun Y,Azad T,Janse van Rensburg HJ,Luo J,Yang S,Liu P,Lv Z,Zhan M,Lu L,Zhou Y,Ma X,Zhang X,Yang X,Xue L

更新日期：2020-12-01 00:00:00

abstract：:Whole-cell and single-channel recording techniques were used to study the action of the anticonvulsant drug MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]- cyclohepten-5,10-imine maleate) on responses to excitatory amino acids in rat neocortical neurons in cell culture. MK-801 caused a progressive, long-lasting bl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huettner JE,Bean BP

更新日期：1988-02-01 00:00:00

abstract：:We have postulated that the toxic neuropathies associated with neurofilament-filled axonal swellings have a common pathogenesis, the covalent crosslinking of neurofilaments during anterograde transport. The newly described gamma-diketone, 3,4-dimethyl-2,5-hexanedione (DMHD), is a more potent analogue of the toxic meta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graham DG,Szakál-Quin G,Priest JW,Anthony DC

更新日期：1984-08-01 00:00:00

abstract：:We have isolated cold-sensitive mutations in two genes of the yeast Saccharomyces cerevisiae, BIN2 and BIN3, that cause aberrant chromosome segregation in vivo. BIN2 and BIN3 encode essential proteins that are similar to each other and to TCP-1. TCP-1 and TCP-1-like proteins are components of the eukaryotic cytoplasmi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Sullivan DS,Huffaker TC

更新日期：1994-09-13 00:00:00

abstract：:Various enzymatic derivatives of the murine myeloma protein IgA(MOPC-315) were subjected to hapten-binding quantitation and circular dichroic analysis in an attempt to ascertain the structural localization and functional definition of the immunoglobulin active site. The extrinsic Cotton effects observed with near satu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Merz DC,Litman GW,Good RA

更新日期：1974-05-01 00:00:00

abstract：:Experimental systemic lupus erythematosus (SLE) can be induced in naive mice by immunization with a murine monoclonal anti-DNA antibody (mAb), 5G12, that bears a major idiotype designated 16/6 Id. Strain-dependent differences were observed in the proliferative responses of lymph node cells of mice immunized with two p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waisman A,Ruiz PJ,Israeli E,Eilat E,Könen-Waisman S,Zinger H,Dayan M,Mozes E

更新日期：1997-04-29 00:00:00

abstract：:Many multifunctional tumor suppressor proteins have low stability, a property linked to cancer development. The von Hippel-Lindau tumor suppressor protein (pVHL) is one of these proteins. pVHL forms part of the E3 ubiquitin ligase complex that regulates the degradation of the hypoxia-inducible factor (HIF). Under nati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Nussinov R

更新日期：2008-01-22 00:00:00

abstract：:Antibody modulation of T-cell coinhibitory (e.g., CTLA-4) or costimulatory (e.g., 4-1BB) receptors promotes clinical responses to a variety of cancers. Therapeutic cancer vaccination, in contrast, has produced limited clinical benefit and no curative therapies. The E6 and E7 oncoproteins of human papilloma virus (HPV)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartkowiak T,Singh S,Yang G,Galvan G,Haria D,Ai M,Allison JP,Sastry KJ,Curran MA

更新日期：2015-09-22 00:00:00

abstract：:Incubation of a human beta-globin pre-mRNA in a HeLa cell nuclear extract under conditions permissive for efficient splicing resulted in the assembly of the RNA into ribonucleoprotein (RNP) complexes. This RNP formation occurred largely within the characteristic lag period that precedes splicing. Two classes of RNP we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayrand SH,Pedersen N,Pederson T

更新日期：1986-06-01 00:00:00

abstract：:Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences. Resi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes DE,Simpson L

更新日期：1986-08-01 00:00:00

abstract：:An intermolecular intercalation of base pairs was found at the CA step in the I222 crystal structure of the RNA.DNA hybrid, r(CAAAGAAAAG).d(CTTTTCTTTG), which contains two-thirds of the polypurine tract sequence of HIV-1 with a substitution of cytosine for the initial adenine. This sequence crystallized in both P21212...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han GW,Kopka ML,Langs D,Sawaya MR,Dickerson RE

更新日期：2003-08-05 00:00:00

abstract：:The leukocyte common antigen (CD45) is an abundant lymphocyte surface antigen that has been reported to be involved in signaling through the T-cell antigen receptor. CD45 is a transmembrane protein-tyrosine-phosphatase. An internal segment comprises two domains each of which is homologous to other protein-tyrosine-pho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stover DR,Charbonneau H,Tonks NK,Walsh KA

更新日期：1991-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cummins JM,He Y,Leary RJ,Pagliarini R,Diaz LA Jr,Sjoblom T,Barad O,Bentwich Z,Szafranska AE,Labourier E,Raymond CK,Roberts BS,Juhl H,Kinzler KW,Vogelstein B,Velculescu VE

更新日期：2006-03-07 00:00:00

abstract：:To study the similarity between a putative cloned mammalian release factor (RF) and tryptophanyl-tRNA synthetase (TRS), a recombinant rabbit RF fusion protein was expressed from prokaryotic expression vectors. Purified fractions of the fusion proteins were tested for TRS and RF activities. Addition of the fusion prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Timchenko L,Caskey CT

更新日期：1994-03-29 00:00:00

abstract：:Although ghrelin has been demonstrated to stimulate energy intake and storage through a central mechanism, its effect on hepatic lipid metabolism remains largely uncharacterized. Ghrelin receptor antagonism or gene deletion significantly decreased obesity-associated hepatic steatosis by suppression of de novo lipogene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Xu G,Qin Y,Zhang C,Tang H,Yin Y,Xiang X,Li Y,Zhao J,Mulholland M,Zhang W

更新日期：2014-09-09 00:00:00

abstract：:Immunization of strain A mice with p-azophenylarsonate-conjugated protein stimulates B cells that synthesize anti-p-azophenylarsonate antibodies. A large fraction of these cells produce antibodies with variable (V) regions encoded by a single heavy chain V gene segment together with multiple combinations of diversity,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wysocki L,Manser T,Gefter ML

更新日期：1986-03-01 00:00:00

abstract：:Amputation of the zebrafish caudal fin stimulates regeneration of the dermal skeleton and reexpression of sonic hedgehog (shh)-signaling pathway genes. Expression patterns suggest a role for shh signaling in the secretion and patterning of the regenerating dermal bone, but a direct role has not been demonstrated. We e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quint E,Smith A,Avaron F,Laforest L,Miles J,Gaffield W,Akimenko MA

更新日期：2002-06-25 00:00:00

abstract：:The effects of messages initiated by one receptor on the covalent modification of a second receptor were studied by use of a technique for rapidly separating the receptors. Methylation of the bacterial-chemotactic serine receptor increases as a result of aspartate binding to the aspartate receptor. The aspartate-induc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanders DA,Koshland DE Jr

更新日期：1988-11-01 00:00:00

abstract：:The C2 domain containing protein extended synaptotagmin (E-Syt) plays important roles in both lipid homeostasis and the intracellular signaling; however, its role in physiology remains largely unknown. Here, we show that hypothalamic E-Syt3 plays a critical role in diet-induced obesity (DIO). E-Syt3 is characteristica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Guan Y,Pan S,Yan L,Wang P,Chen Z,Shen Q,Zhao F,Zhang X,Li J,Li J,Cai D,Zhang G

更新日期：2020-08-18 00:00:00

abstract：:We developed a method for rapidly generating thermostable enzyme variants. Our strategy is to introduce the gene coding for a given enzyme from a mesophilic organism into a thermophile, Bacillus stearothermophilus. Variants that retain the enzymatic activity at the higher growth temperatures of the thermophile are the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao H,McKenzie T,Hageman R

更新日期：1986-02-01 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were exposed to various thiol-directed spin labels, and the position of the label on the inner or outer vesicle surface was investigated as a function of the ATPase (adenosinetriphosphatase; ATP phosphohydrolase, EC 3.6.1.3) chemical state. Previous measurements of label accessibility t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champeil P,Rigaud JL,Gary-Bobo CM

更新日期：1980-05-01 00:00:00

abstract：:Phyllosphere microbial communities were evaluated on leaves of field-grown plant species by culture-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) with 16S rDNA primers generally indicated that microbial community structures were similar on different individuals of the same plant sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang CH,Crowley DE,Borneman J,Keen NT

更新日期：2001-03-27 00:00:00

abstract：:RNA-protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA-protein interactions in living cells in an unbiased manner. We used two approaches to target the engineered peroxidase APEX2 to specific cellular RNAs for RNA-centered proximity biotinylation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han S,Zhao BS,Myers SA,Carr SA,He C,Ting AY

更新日期：2020-09-08 00:00:00

abstract：:A cell of the bacterium Escherichia coli was tethered covalently to a glass coverslip by a single flagellum, and its rotation was stopped by using optical tweezers. The tweezers acted directly on the cell body or indirectly, via a trapped polystyrene bead. The torque generated by the flagellar motor was determined by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berry RM,Berg HC

更新日期：1997-12-23 00:00:00

abstract：:There is pronounced seasonal plasticity in the morphology of the neural circuits that regulate song behavior in adult songbirds, primarily in response to changes in plasma testosterone (T) levels. Most song nuclei have androgen receptors. Afferent input from the telencephalic nucleus HVc (also known as the "high vocal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brenowitz EA,Lent K

更新日期：2002-09-17 00:00:00