Hypothalamic extended synaptotagmin-3 contributes to the development of dietary obesity and related metabolic disorders.

abstract：:The ability to track cells and their patterns of gene expression in living organisms can increase our understanding of tissue development and disease. Gene reporters for bioluminescence, fluorescence, radionuclide, and magnetic resonance imaging (MRI) have been described but these suffer variously from limited depth p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patrick PS,Hammersley J,Loizou L,Kettunen MI,Rodrigues TB,Hu DE,Tee SS,Hesketh R,Lyons SK,Soloviev D,Lewis DY,Aime S,Fulton SM,Brindle KM

更新日期：2014-01-07 00:00:00

abstract：:Aspergillus fumigatus is commonly associated with allergic bronchopulmonary aspergillosis in patients with severe asthma in which chronic airway neutrophilia predicts a poor outcome. We were able to recapitulate fungus-induced neutrophilic airway inflammation in a mouse model in our efforts to understand the underlyin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fei M,Bhatia S,Oriss TB,Yarlagadda M,Khare A,Akira S,Saijo S,Iwakura Y,Fallert Junecko BA,Reinhart TA,Foreman O,Ray P,Kolls J,Ray A

更新日期：2011-03-29 00:00:00

abstract：:Chondrules may have played a critical role in the earliest stages of planet formation by mediating the accumulation of dust into planetesimals. However, the origin of chondrules and their significance for planetesimal accretion remain enigmatic. Here, we show that chondrules and matrix in the carbonaceous chondrite Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Budde G,Kleine T,Kruijer TS,Burkhardt C,Metzler K

更新日期：2016-03-15 00:00:00

abstract：:Hippocampal mossy fibers, which are the axons of dentate granule cells, form powerful excitatory synapses onto the proximal dendrites of CA3 pyramidal cells. It has long been known that high-affinity binding sites for kainate, a glutamate receptor agonist, are present on mossy fibers. Here we summarize recent experime...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmitz D,Mellor J,Frerking M,Nicoll RA

更新日期：2001-09-25 00:00:00

abstract：:Selection of mammalian cells in progressively increasing concentrations of methotrexate results in selective amplification of DNA sequences coding for dihydrofolate reductase (tetrahydrofolate dehydrogenase, 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3). In some cell variants the amplified genes are stabl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufman RJ,Brown PC,Schimke RT

更新日期：1979-11-01 00:00:00

abstract：:Ubiquitin is a highly conserved protein that is encoded by a multigene family. It is generally believed that this gene family is subject to concerted evolution, which homogenizes the member genes of the family. However, protein homogeneity can be attained also by strong purifying selection. We therefore studied the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nei M,Rogozin IB,Piontkivska H

更新日期：2000-09-26 00:00:00

abstract：:This study demonstrates that significantly shortened telomeres are a hallmark of cardiomyocytes (CMs) from individuals with end-stage hypertrophic cardiomyopathy (HCM) or dilated cardiomyopathy (DCM) as a result of heritable defects in cardiac proteins critical to contractile function. Positioned at the ends of chromo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang ACY,Chang ACH,Kirillova A,Sasagawa K,Su W,Weber G,Lin J,Termglinchan V,Karakikes I,Seeger T,Dainis AM,Hinson JT,Seidman J,Seidman CE,Day JW,Ashley E,Wu JC,Blau HM

更新日期：2018-09-11 00:00:00

abstract：:The study of gene family members has been aided by the isolation of related genes on the basis of DNA homology. We have adapted the polymerase chain reaction to screen animal genomes very rapidly and reliably for likely gene family members. Using conserved amino acid sequences to design degenerate oligonucleotide prim...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamb A,Weir M,Rudy B,Varmus H,Kenyon C

更新日期：1989-06-01 00:00:00

abstract：:Heterosis is widely applied in agriculture; however, the underlying molecular mechanisms for superior performance are not well understood. Ethylene biosynthesis and signaling genes are shown to be down-regulated in Arabidopsis interspecific hybrids. Ethylene is a plant hormone that promotes fruit ripening and maturati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song Q,Ando A,Xu D,Fang L,Zhang T,Huq E,Qiao H,Deng XW,Chen ZJ

更新日期：2018-05-22 00:00:00

abstract：:We have developed an in vitro transcription system that uses nuclei isolated from Lemna gibba G-3. The in vitro transcripts include sequences homologous to hybridization probes for the small subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39], the light-harv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silverthorne J,Tobin EM

更新日期：1984-02-01 00:00:00

abstract：:Polymorphism may play an important role in speciation because new species could originate from the distinctive morphs observed in polymorphic populations. However, much remains to be understood about the process by which morphs found new species. To detail the steps of this mode of speciation, we studied the geographi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Corl A,Davis AR,Kuchta SR,Sinervo B

更新日期：2010-03-02 00:00:00

abstract：:Alzheimer's disease (AD) is the most prevalent of a large group of related proteinopathies for which there is currently no cure. Here, we used Drosophila to explore a strategy to block Aβ42 neurotoxicity through engineering of the Heat shock protein 70 (Hsp70), a chaperone that has demonstrated neuroprotective activit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandez-Funez P,Sanchez-Garcia J,de Mena L,Zhang Y,Levites Y,Khare S,Golde TE,Rincon-Limas DE

更新日期：2016-08-30 00:00:00

abstract：:Recent advances in electrodes for noninvasive recording of electroencephalograms expand opportunities collecting such data for diagnosis of neurological disorders and brain-computer interfaces. Existing technologies, however, cannot be used effectively in continuous, uninterrupted modes for more than a few days due to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Norton JJ,Lee DS,Lee JW,Lee W,Kwon O,Won P,Jung SY,Cheng H,Jeong JW,Akce A,Umunna S,Na I,Kwon YH,Wang XQ,Liu Z,Paik U,Huang Y,Bretl T,Yeo WH,Rogers JA

更新日期：2015-03-31 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) signaling is thought to mediate quorum sensing in many species of Proteobacteria. The opportunistic human pathogen Pseudomonas aeruginosa uses acyl-HSLs to regulate hundreds of genes, including many that code for extracellular virulence factors. The idea that the P. aeruginosa acyl-H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yarwood JM,Volper EM,Greenberg EP

更新日期：2005-06-21 00:00:00

abstract：:The basic assumptions of van der Waals theory are contained in two well-known concepts: excluded volume (repulsive forces) and a homogeneous, isotropic field potential (attractive forces). We have superimposed on these, one more well-known concept: the existence of dimers, trimers, etc., at chemical equilibrium. With ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heidemann RA,Prausnitz JM

更新日期：1976-06-01 00:00:00

abstract：:We introduce a paradigm-"hydricity"-that involves the coproduction of hydrogen and electricity from solar thermal energy and their judicious use to enable a sustainable economy. We identify and implement synergistic integrations while improving each of the two individual processes. When the proposed integrated process...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gençer E,Mallapragada DS,Maréchal F,Tawarmalani M,Agrawal R

更新日期：2015-12-29 00:00:00

abstract：:Antibody against carcinoembryonic antigen (CEA) was conjugated with p-[1,2-dicarba-closo-[1-3H]do-decaboran(12)-2-yl] benzenediazonium ion by an azo-coupling reaction, resulting in 30 boron atoms per IgG molecule with no loss of antibody protein. Antibody immunoreactivity was not appreciably affected by this conjugati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldenberg DM,Sharkey RM,Primus FJ,Mizusawa E,Hawthorne MF

更新日期：1984-01-01 00:00:00

abstract：:Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences. Resi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes DE,Simpson L

更新日期：1986-08-01 00:00:00

abstract：:Reducible compounds that participate in crosslinking were analyzed in hydroxylysine-deficient collagens of patients with a heritable disorder of connective tissue. After treatment with [(3)H]sodium borohydride, new compounds, as well as a totally different pattern of tritiated compounds, were found in hydroxylysine-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eyre DR,Glimcher MJ

更新日期：1972-09-01 00:00:00

abstract：:Lipoxin B (LXB) was prepared by incubation of (15S)-15-hydroperoxy-5,8,11-cis-13-trans-icosatetraenoic acid (15-HPETE) with human leukocytes. Comparison with a number of trihydroxyicosatetraenes prepared by total synthesis showed that biologically derived LXB is (5S,14R,15S)-5,14,15-trihydroxy-6,10,12-trans-8-cis-icos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Serhan CN,Hamberg M,Samuelsson B,Morris J,Wishka DG

更新日期：1986-04-01 00:00:00

abstract：:Single molecule studies, at constant force, of the separation of double-stranded DNA into two separated single strands may provide information relevant to the dynamics of DNA replication. At constant applied force, theory predicts that the unzipped length as a function of time is characterized by jumps during which th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Danilowicz C,Coljee VW,Bouzigues C,Lubensky DK,Nelson DR,Prentiss M

更新日期：2003-02-18 00:00:00

abstract：:A fundamental aspect of climate change is the potential shifts in flowering phenology and pollen initiation associated with milder winters and warmer seasonal air temperature. Earlier floral anthesis has been suggested, in turn, to have a role in human disease by increasing time of exposure to pollen that causes aller...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ziska L,Knowlton K,Rogers C,Dalan D,Tierney N,Elder MA,Filley W,Shropshire J,Ford LB,Hedberg C,Fleetwood P,Hovanky KT,Kavanaugh T,Fulford G,Vrtis RF,Patz JA,Portnoy J,Coates F,Bielory L,Frenz D

更新日期：2011-03-08 00:00:00

abstract：:Approximately 15 per cent of the light chains from homogeneous immunoglobulins in patients with multiple myeloma contain an oligosaccharide group. Five human myeloma light chains that contained carbohydrate were studied. The sequence Asn-[unk]-Ser/Thr was at the site of carbohydrate attachment in all light chains. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sox HC Jr,Hood L

更新日期：1970-07-01 00:00:00

abstract：:The Schiff base adducts of glyceraldehyde with hemoglobin undergo Amadori rearrangement to form stable ketoamine structures; this reaction is similar to the nonenzymic glucosylation of proteins. In the present studies the analogous rearrangement of the Schiff base adducts of glycolaldehyde with proteins has been demon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acharya AS,Manning JM

更新日期：1983-06-01 00:00:00

abstract：:The effect of pressure up to 175 kilobars on the electronic structure of three ferric hydroxamates and on ferrichrome A has been studied by optical absorption and Mössbauer resonance. The ferric ion was reduced to ferrous ion with pressure, as has been previously observed for various compounds. For the hydroxamates, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grenoble DC,Drickamer HG

更新日期：1971-03-01 00:00:00

abstract：:For many agronomically important plant genes, only their position on a genetic map is known. In the absence of an efficient transposon tagging system, such genes have to be isolated by map-based cloning. In bread wheat Triticum aestivum, the genome is hexaploid, has a size of 1.6 x 10(10) bp, and contains more than 80...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stein N,Feuillet C,Wicker T,Schlagenhauf E,Keller B

更新日期：2000-11-21 00:00:00

abstract：:The members of the cytoplasmic 70-kDa heat shock protein family are involved in appropriate folding and trafficking of newly synthesized proteins in the cell. Hsc70, which is expressed constitutively, and Hsp70, the expression of which is stress- and heat shock-induced, are often considered to have similar cellular fu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldfarb SB,Kashlan OB,Watkins JN,Suaud L,Yan W,Kleyman TR,Rubenstein RC

更新日期：2006-04-11 00:00:00

abstract：:The inability of adenovirus to infect primitive hematopoietic cells presents an obstacle to the use of adenovirus vectors for gene transfer to these cell types. Therefore, expanding the tropism of adenovirus vectors to unique cell surface antigens would be an important development for gene therapy protocols. In this s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith JS,Keller JR,Lohrey NC,McCauslin CS,Ortiz M,Cowan K,Spence SE

更新日期：1999-08-03 00:00:00

abstract：:Rabbit corneas denuded of their endothelium were coated with bovine corneal endothelial cells (from steers) previously maintained in tissue culture for short (20 generations) or prolonged (200 generations) periods. When grafted back into female rabbits, the corneal buttons remained clear and showed no edema. In contra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gospodarowicz D,Greenburg G,Alvarado J

更新日期：1979-01-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) viral DNA synthesis in quiescent and activated peripheral blood lymphocytes (PBLs) was studied. Incomplete viral DNA (previously demonstrated to be associated with HIV-1 virions) is carried by HIV-1 virions into quiescent and activated PBLs, contributing to the formation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao WY,Cara A,Gallo RC,Lori F

更新日期：1993-10-01 00:00:00