Abstract:
:Single molecule studies, at constant force, of the separation of double-stranded DNA into two separated single strands may provide information relevant to the dynamics of DNA replication. At constant applied force, theory predicts that the unzipped length as a function of time is characterized by jumps during which the strands separate rapidly, followed by long pauses where the number of separated base pairs remains constant. Here, we report previously uncharacterized observations of this striking behavior carried out on a number of identical single molecules simultaneously. When several single lambda phage molecules are subject to the same applied force, the pause positions are reproducible in each. This reproducibility shows that the positions and durations of the pauses in unzipping provide a sequence-dependent molecular fingerprint. For small forces, the DNA remains in a partially unzipped state for at least several hours. For larger forces, the separation is still characterized by jumps and pauses, but the double-stranded DNA will completely unzip in less than 30 min.
journal_name
Proc Natl Acad Sci U S Aauthors
Danilowicz C,Coljee VW,Bouzigues C,Lubensky DK,Nelson DR,Prentiss Mdoi
10.1073/pnas.262789199keywords:
subject
Has Abstractpub_date
2003-02-18 00:00:00pages
1694-9issue
4eissn
0027-8424issn
1091-6490pii
262789199journal_volume
100pub_type
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