Abstract:
:Prions are infectious proteins that encipher biological information within their conformations; variations in these conformations dictate different prion strains. Toward elucidating the molecular language of prion protein (PrP) conformations, we produced an array of recombinant PrP amyloids with varying conformational stabilities. In mice, the most stable amyloids produced the most stable prion strains that exhibited the longest incubation times, whereas more labile amyloids generated less stable strains and shorter incubation times. The direct relationship between stability and incubation time of prion strains suggests that labile prions are more fit, in that they accumulate more rapidly and thus kill the host faster. Although incubation times can be changed by altering the PrP expression level, PrP sequence, prion dose, or route of inoculation, we report here the ability to modify the incubation time predictably in mice by modulating the prion conformation.
journal_name
Proc Natl Acad Sci U S Aauthors
Colby DW,Giles K,Legname G,Wille H,Baskakov IV,DeArmond SJ,Prusiner SBdoi
10.1073/pnas.0910350106subject
Has Abstractpub_date
2009-12-01 00:00:00pages
20417-22issue
48eissn
0027-8424issn
1091-6490pii
0910350106journal_volume
106pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2015-04-21 00:00:00