Cathepsin B: association with plasma membrane in metastatic tumors.

abstract：:Skeletal muscle fibers are defined by patterned covariation of key traits that determine contractile and metabolic characteristics. Although the functional properties of most skeletal muscles result from their proportional content of a few conserved muscle fiber types, some, typically craniofacial, muscles exhibit fib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porter JD,Khanna S,Kaminski HJ,Rao JS,Merriam AP,Richmonds CR,Leahy P,Li J,Andrade FH

更新日期：2001-10-09 00:00:00

abstract：:The human CMV UL37x1-encoded protein, also known as the viral mitochondria-localized inhibitor of apoptosis, traffics to the endoplasmic reticulum and mitochondria of infected cells. It induces the fragmentation of mitochondria and blocks apoptosis. We demonstrate that UL37x1 protein mobilizes Ca(2+) from the endoplas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharon-Friling R,Goodhouse J,Colberg-Poley AM,Shenk T

更新日期：2006-12-12 00:00:00

abstract：:O-GlcNAc glycosylation (or O-GlcNAcylation) is a dynamic, inducible posttranslational modification found on proteins associated with neurodegenerative diseases such as α-synuclein, amyloid precursor protein, and tau. Deletion of the O-GlcNAc transferase (ogt) gene responsible for the modification causes early postnata...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang AC,Jensen EH,Rexach JE,Vinters HV,Hsieh-Wilson LC

更新日期：2016-12-27 00:00:00

abstract：:Glucocorticoids are commonly used antiinflammatory agents whose use is limited by side effects. We have developed a series of glucocorticoid receptor (GR) ligands that retain the strong antiinflammatory activity of conventional glucocorticoids with reduced side effects. We present a compound, LGD5552, that binds the r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miner JN,Ardecky B,Benbatoul K,Griffiths K,Larson CJ,Mais DE,Marschke K,Rosen J,Vajda E,Zhi L,Negro-Vilar A

更新日期：2007-12-04 00:00:00

abstract：:The structure of the gene encoding human hexabrachion (tenascin) has been determined from overlapping clones isolated from a human genomic bacteriophage library. The genomic inserts were characterized by restriction mapping, Southern blot analysis, PCR, and DNA sequencing. The coding region of the hexabrachion gene sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gulcher JR,Nies DE,Alexakos MJ,Ravikant NA,Sturgill ME,Marton LS,Stefansson K

更新日期：1991-11-01 00:00:00

abstract：:The blue-light receptor genes (sopII) of sensory rhodopsin (SR) II were cloned from two species, the halophilic bacteria Haloarcula vallismortis (vSR-II) and Natronobacterium pharaonis (pSR-II). Upstream of both sopII gene loci, sequences corresponding to the halobacterial transducer of rhodopsin (Htr) II were recogni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seidel R,Scharf B,Gautel M,Kleine K,Oesterhelt D,Engelhard M

更新日期：1995-03-28 00:00:00

abstract：:A fragment of the cloned gene for the human myeloma ND epsilon chain, coding for the second, third, and fourth domains of the immunoglobulin, has been coupled to the tryptophan control region of an expression plasmid and subcloned in Escherichia coli. Induction of gene expression results in the synthesis of the expect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kenten J,Helm B,Ishizaka T,Cattini P,Gould H

更新日期：1984-05-01 00:00:00

abstract：:The p21 region of human chromosome 9 is thought to contain a gene (MLM) involved in genetic susceptibility to melanoma and a gene or genes that influence progression of certain other tumors. Genomic clones that span a large region in 9p21 surrounding the presumptive tumor suppressor gene(s) have been isolated. A set o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weaver-Feldhaus J,Gruis NA,Neuhausen S,Le Paslier D,Stockert E,Skolnick MH,Kamb A

更新日期：1994-08-02 00:00:00

abstract：:Thyroid hormone (TH) metabolism, mediated by deiodinase types 1, 2, and 3 (D1, D2, and D3) is profoundly affected by acute illness. We examined the role of TH metabolism during ventilator-induced lung injury (VILI) in mice. Mice exposed to VILI recapitulated the serum TH findings of acute illness, namely a decrease in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barca-Mayo O,Liao XH,DiCosmo C,Dumitrescu A,Moreno-Vinasco L,Wade MS,Sammani S,Mirzapoiazova T,Garcia JG,Refetoff S,Weiss RE

更新日期：2011-12-06 00:00:00

abstract：:The nonclassical MHC class I-related (MHC-I) molecule HFE controls cellular iron homeostasis by a mechanism that has not been fully elucidated. We examined the regulation of HFE by K5, the E3 ubiquitin ligase encoded by Kaposi's sarcoma-associated herpesvirus (KSHV/HHV8), that is known to down-regulate classical MHC-I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rhodes DA,Boyle LH,Boname JM,Lehner PJ,Trowsdale J

更新日期：2010-09-14 00:00:00

abstract：:Somatic cell hybridization of mouse erythroleukemia (MEL) cells and HEL cells, a human erythroleukemia line that produces fetal (gamma) but fails to express adult (beta) globin, was used to test whether the expression of the two human globin genes is regulated cis or trans. An experimental approach using anti-human gl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papayannopoulou TH,Lindsley D,Kurachi S,Lewison K,Hemenway T,Melis M,Anagnou NP,Najfeld V

更新日期：1985-02-01 00:00:00

abstract：:The inositol 1,4,5-trisphosphate receptor (IP3R) associated with plasma membranes of lymphocytes differs in terminal sugar content and binding specificity from the cerebellar receptor, which is localized to endoplasmic reticulum. Lectin column chromatography reveals that 30% of IP3R in the thymus contains sialic acid,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan AA,Steiner JP,Snyder SH

更新日期：1992-04-01 00:00:00

abstract：:Lesion of the sciatic nerve caused a rapid increase in c-fos and c-jun mRNA that was followed about 2 hr later by an increase in nerve growth factor (NGF) mRNA. To evaluate whether the initial increase in c-fos mRNA is causally related to the subsequent increase in NGF mRNA, we performed experiments with fibroblasts o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hengerer B,Lindholm D,Heumann R,Rüther U,Wagner EF,Thoenen H

更新日期：1990-05-01 00:00:00

abstract：:Here, we highlight the potential translational benefits of delivering FLASH radiotherapy using ultra-high dose rates (>100 Gy⋅s-1). Compared with conventional dose-rate (CONV; 0.07-0.1 Gy⋅s-1) modalities, we showed that FLASH did not cause radiation-induced deficits in learning and memory in mice. Moreover, 6 months a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Montay-Gruel P,Acharya MM,Petersson K,Alikhani L,Yakkala C,Allen BD,Ollivier J,Petit B,Jorge PG,Syage AR,Nguyen TA,Baddour AAD,Lu C,Singh P,Moeckli R,Bochud F,Germond JF,Froidevaux P,Bailat C,Bourhis J,Vozenin MC

更新日期：2019-05-28 00:00:00

abstract：:The role of tumor necrosis factor alpha (TNF-alpha) in the pathogenesis of autoimmune diabetes mellitus was tested in the nonobese mouse (NOD) model system. The effects of TNF-alpha were assessed on three levels: (i) insulitis development, (ii) development of overt diabetes, (iii) adoptive transfer of diabetes by sple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacob CO,Aiso S,Michie SA,McDevitt HO,Acha-Orbea H

更新日期：1990-02-01 00:00:00

abstract：:The development of the luminescence system in Beneckea harveyi is controlled by cyclic nucleotides at the level of transcription. In the wild type, it is repressed by exogenously added guanosine 3':5'-cyclic monophosphate and this repression is overcome by the addition of adenosine 3':5'-cyclic monophosphate. These ob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ulitzur S,Yashphe J,Hastings JW

更新日期：1976-12-01 00:00:00

abstract：:There is accumulating evidence that AKT signaling plays a role in the pathogenesis of schizophrenia. We asked whether Akt1 deficiency in mice results in structural and functional abnormalities in prefrontal cortex (PFC). Exploratory transcriptional profiling revealed concerted alterations in the expression of PFC gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai WS,Xu B,Westphal KG,Paterlini M,Olivier B,Pavlidis P,Karayiorgou M,Gogos JA

更新日期：2006-11-07 00:00:00

abstract：:Minimally invasive detection of cell death could prove an invaluable resource in many physiologic and pathologic situations. Cell-free circulating DNA (cfDNA) released from dying cells is emerging as a diagnostic tool for monitoring cancer dynamics and graft failure. However, existing methods rely on differences in DN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehmann-Werman R,Neiman D,Zemmour H,Moss J,Magenheim J,Vaknin-Dembinsky A,Rubertsson S,Nellgård B,Blennow K,Zetterberg H,Spalding K,Haller MJ,Wasserfall CH,Schatz DA,Greenbaum CJ,Dorrell C,Grompe M,Zick A,Hubert A,M

更新日期：2016-03-29 00:00:00

abstract：:Poliovirus RNA polymerase requires a host factor to initiate RNA synthesis in vitro. The host factor was previously purified to near homogeneity from HeLa cells but was not assigned an enzymatic activity. This report describes the purification of a terminal uridylyltransferase that can act as host factor. By all crite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andrews NC,Baltimore D

更新日期：1986-01-01 00:00:00

abstract：:Under conditions (0.2% CO2; 1% O2) that allow high rates of photosynthesis, chlorophyll fluorescence was measured simultaneously with carbon assimilation at various light intensities in spinach (Spinacia oleracea) leaves. Using a stoichiometry of 3 ATP/CO2 and the known relationship between ATP synthesis rate and driv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schönknecht G,Neimanis S,Katona E,Gerst U,Heber U

更新日期：1995-12-19 00:00:00

abstract：:The E1A gene of adenovirus type 5 encodes two major proteins of 289 and 243 amino acid residues, which are identical except that the larger protein has an internal stretch of 46 amino acids required for efficient trans-activation of early viral promoters. This domain contains a consensus zinc finger motif (Cys-Xaa2-Cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Culp JS,Webster LC,Friedman DJ,Smith CL,Huang WJ,Wu FY,Rosenberg M,Ricciardi RP

更新日期：1988-09-01 00:00:00

abstract：:Agrobacterium tumefaciens causes crown gall tumors on various plants by delivering transferred DNA (T-DNA) and virulence proteins into host plant cells. Under laboratory conditions, the bacterium is widely used as a vector to genetically modify a wide range of organisms, including plants, yeasts, fungi, and algae. Var...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Q,Li X,Tu H,Pan SQ

更新日期：2017-03-14 00:00:00

abstract：:Previously, recombinant human respiratory syncytial virus (RSV) monoclonal antibody Fabs were generated by antigen selection from random combinatorial libraries displayed at the tip of filamentous phage. Two such Fabs, which exhibited high binding affinity for RSV F glycoprotein (a major protective antigen), were eval...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crowe JE Jr,Murphy BR,Chanock RM,Williamson RA,Barbas CF 3rd,Burton DR

更新日期：1994-02-15 00:00:00

abstract：:A type of chromosome-free cell called SimCells (simple cells) has been generated from Escherichia coli, Pseudomonas putida, and Ralstonia eutropha. The removal of the native chromosomes of these bacteria was achieved by double-stranded breaks made by heterologous I-CeuI endonuclease and the degradation activity of end...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan C,Davison PA,Habgood R,Zeng H,Decker CM,Gesell Salazar M,Lueangwattanapong K,Townley HE,Yang A,Thompson IP,Ye H,Cui Z,Schmidt F,Hunter CN,Huang WE

更新日期：2020-03-24 00:00:00

abstract：:A full-length cDNA for tryptophan hydroxylase was cloned from rabbit pineal body by screening an expression library with antibody against rat phenylalanine hydroxylase, which crossreacts with rabbit tryptophan hydroxylase. Clones producing immunoreactive material contain sequences homologous to, yet distinct from, phe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grenett HE,Ledley FD,Reed LL,Woo SL

更新日期：1987-08-01 00:00:00

abstract：:These studies sought to determine if neurons in the estrogen receptor-alpha knockout (ERalphaKO) mouse brain concentrated 16alpha-[125I]iodo-11beta-methoxy-17beta-estradiol (125I-estrogen), and if so, whether estrogen binding augmented the expression of progesterone receptor (PR) mRNA. Mice were injected with 125I-est...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shughrue PJ,Lubahn DB,Negro-Vilar A,Korach KS,Merchenthaler I

更新日期：1997-09-30 00:00:00

abstract：:We have attempted to model human metastatic disease by implanting human target organs into the immunodeficient C.B-17 scid/scid (severe combined immunodeficiency; SCID) mouse, creating SCID-hu mice. Preferential metastasis to implants of human fetal lung and human fetal bone marrow occurred after i.v. injection of hum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shtivelman E,Namikawa R

更新日期：1995-05-09 00:00:00

abstract：:Aptamers recognize their targets with extraordinary affinity and specificity. The aptamer-based therapeutic, Macugen, is derived from a modified 2'fluoro pyrimidine RNA inhibitor to vascular endothelial growth factor (VEGF) and is now being used to treat the wet form of age-related macular degeneration. This VEGF(165)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee JH,Canny MD,De Erkenez A,Krilleke D,Ng YS,Shima DT,Pardi A,Jucker F

更新日期：2005-12-27 00:00:00

abstract：:Ocean acidification (OA) refers to the ongoing decline in oceanic pH resulting from the uptake of atmospheric CO(2). Mounting experimental evidence suggests that OA will have negative consequences for a variety of marine organisms. Whereas the effect of OA on the calcification of adult reef corals is increasingly well...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albright R,Mason B,Miller M,Langdon C

更新日期：2010-11-23 00:00:00

abstract：:Biological resurfacing of entire articular surfaces represents an important but challenging strategy for treatment of cartilage degeneration that occurs in osteoarthritis. Not only does this approach require anatomically sized and functional engineered cartilage, but the inflammatory environment within an arthritic jo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moutos FT,Glass KA,Compton SA,Ross AK,Gersbach CA,Guilak F,Estes BT

更新日期：2016-08-02 00:00:00