Specific disruption of vimentin filament organization in monkey kidney CV-1 cells by diphtheria toxin, exotoxin A, and cycloheximide.

abstract：:We are investigating the feasibility of using the positive-strand RNA virus Sindbis virus and its defective interfering (DI) particles as vectors for introducing foreign genes into cells. In previous work we showed by deletion mapping of a cloned cDNA derived from one of the DI RNAs that only nucleotides at the 3' and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levis R,Huang H,Schlesinger S

更新日期：1987-07-01 00:00:00

abstract：:Effective gene therapy depends on the efficient transfer of therapeutic genes and their protein products to target cells. Lentiviral vectors appear promising for virus-mediated gene delivery and long-term expression in nondividing cells. The herpes simplex virus type 1 tegument protein VP22 has recently been shown to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai Z,Han I,Zirzow G,Brady RO,Reiser J

更新日期：2000-10-10 00:00:00

abstract：:There are defined medullary, mesencephalic, hypothalamic, and thalamic functions in regulation of respiration, but knowledge of cortical control and the elements subserving the consciousness of breathlessness and air hunger is limited. In nine young adults, air hunger was produced acutely by CO(2) inhalation. Comparis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brannan S,Liotti M,Egan G,Shade R,Madden L,Robillard R,Abplanalp B,Stofer K,Denton D,Fox PT

更新日期：2001-02-13 00:00:00

abstract：:High-resolution magnetic resonance microscopy (MRM) was used to determine regional brain volumetric changes in a mouse model of Alzheimer's disease. These transgenic (Tg) mice overexpress human mutant amyloid precursor protein (APP) V717F under control of platelet-derived growth factor promoter (PDAPP mice), and corti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Redwine JM,Kosofsky B,Jacobs RE,Games D,Reilly JF,Morrison JH,Young WG,Bloom FE

更新日期：2003-02-04 00:00:00

abstract：:Alveolar type II (AT2) cell dysfunction contributes to a number of significant human pathologies including respiratory distress syndrome, lung adenocarcinoma, and debilitating fibrotic diseases, but the critical transcription factors that maintain AT2 cell identity are unknown. Here we show that the E26 transformation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Z,Newton K,Kummerfeld SK,Webster J,Kirkpatrick DS,Phu L,Eastham-Anderson J,Liu J,Lee WP,Wu J,Li H,Junttila MR,Dixit VM

更新日期：2017-04-11 00:00:00

abstract：:Human workers ensure the functioning of governments around the world. The efficacy of human workers, in turn, is linked to the climatic conditions they face. Here we show that the same weather that amplifies human health hazards also reduces street-level government workers' oversight of these hazards. To do so, we emp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Obradovich N,Tingley D,Rahwan I

更新日期：2018-08-28 00:00:00

abstract：:A brilliant, coarsely granular nuclear antigen was detected by anti-complement immunofluorescence in the nuclei of acute myeloid leukemia myeloblasts. Designated as LANA (leukemia-associated nuclear antigen), the reactivity differs from that of the Epstein-Barr-virus-determined nuclear antigen (EBNA) in immunological ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein G,Steiner M,Wiener F,Klein E

更新日期：1974-03-01 00:00:00

abstract：:AMP deaminase (adenylate deaminase; AMP aminohydrolase, EC 3.5.4.6), a large flat tetrameric enzyme found in skeletal muscle, binds strongly and specifically to the subfragment-2 region of rabbit skeletal muscle myosin. This allows its use as a structural probe in myosin and myosin rod aggregation studies. When mixed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koretz JF

更新日期：1982-10-01 00:00:00

abstract：:The imino sugar N-butyldeoxynojirimycin (NBDNJ) is a potent inhibitor of the oligosaccharide-trimming enzyme alpha-glucosidase I. Hepatitis B virus (HBV) contains three surface proteins (HBs proteins) of different sizes that are singly or doubly N-glycosylated and are essential for the formation of infectious virus. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Block TM,Lu X,Platt FM,Foster GR,Gerlich WH,Blumberg BS,Dwek RA

更新日期：1994-03-15 00:00:00

abstract：:The vacuolar (H(+))-ATPases (V-ATPases) are ATP-dependent proton pumps that acidify intracellular compartments and pump protons across specialized plasma membranes. Proton translocation occurs through the integral V(0) domain, which contains five different subunits (a, d, c, c', and c"). Proton transport is critically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawasaki-Nishi S,Nishi T,Forgac M

更新日期：2001-10-23 00:00:00

abstract：:Vacuoles are essential organelles in plants, playing crucial roles, such as cellular material degradation, ion and metabolite storage, and turgor maintenance. Vacuoles receive material via the endocytic, secretory, and autophagic pathways. Membrane fusion is the last step during which prevacuolar compartments (PVCs) a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rodriguez-Furlan C,Domozych D,Qian W,Enquist PA,Li X,Zhang C,Schenk R,Winbigler HS,Jackson W,Raikhel NV,Hicks GR

更新日期：2019-10-15 00:00:00

abstract：:Regulation of the turnover of triglycerides in adipose tissue requires the continuous provision of 3-glycerophosphate, which may be supplied by the metabolism of glucose or by glyceroneogenesis, the de novo synthesis of 3-glycerophosphate from sources other than hexoses or glycerol. The importance of glyceroneogenesis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olswang Y,Cohen H,Papo O,Cassuto H,Croniger CM,Hakimi P,Tilghman SM,Hanson RW,Reshef L

更新日期：2002-01-22 00:00:00

abstract：:Mutations in genes encoding membrane proteins have been associated with cell death of unknown cause from invertebrate development to human degenerative diseases. A point mutation in the gene for myelin proteolipid protein (PLP) underlies oligodendrocyte death and dysmyelination in jimpy mice, an accurate model for Pel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schneider AM,Griffiths IR,Readhead C,Nave KA

更新日期：1995-05-09 00:00:00

abstract：:Ultraviolet irradiation of the [3H]colchicine-tubulin complex leads to direct photolabeling of tubulin with low but practicable efficiency. The bulk (70% to greater than 90%) of the labeling occurs on beta-tubulin and appears early after irradiation, whereas alpha-tubulin is labeled later. The labeling ratio of beta-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolff J,Knipling L,Cahnmann HJ,Palumbo G

更新日期：1991-04-01 00:00:00

abstract：:Poly([14C]adenosine diphosphate ribose) was synthesized from [14C]NAD+ with calf thymus nuclei. The fraction containing poly(adenosine diphosphate ribose) eluted with 0.22--0.40 M phosphate buffer (pH 6.8) from a hydroxylapatite column, was completely hydrolyzed with venom phosphodiesterase, and was separated by DEAE-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miwa M,Saikawa N,Yamaizumi Z,Nishimura S,Sugimura T

更新日期：1979-02-01 00:00:00

abstract：:The evolution of human languages is driven both by primitive biases present in the human sensorimotor systems and by cultural transmission among speakers. However, whether the design of the language faculty is further shaped by linguistic biological biases remains controversial. To address this question, we used near-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gómez DM,Berent I,Benavides-Varela S,Bion RA,Cattarossi L,Nespor M,Mehler J

更新日期：2014-04-22 00:00:00

abstract：:Voltage-dependent potassium channels (Kv) are homotetramers composed of four voltage sensors and one pore domain. Because of high-level structural flexibility, the first mammalian Kv structure, Kv1.2 at 2.9 A, has about 37% molecular mass of the transmembrane portion not resolved. In this study, by applying a novel no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Wang Q,Ni F,Ma J

更新日期：2010-06-22 00:00:00

abstract：:We propose and test a theoretical perspective in which a universal hallmark of successful literacy acquisition is the convergence of the speech and orthographic processing systems onto a common network of neural structures, regardless of how spoken words are represented orthographically in a writing system. During fun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rueckl JG,Paz-Alonso PM,Molfese PJ,Kuo WJ,Bick A,Frost SJ,Hancock R,Wu DH,Mencl WE,Duñabeitia JA,Lee JR,Oliver M,Zevin JD,Hoeft F,Carreiras M,Tzeng OJ,Pugh KR,Frost R

更新日期：2015-12-15 00:00:00

abstract：:The embryonic neuroepithelium gives rise to the components of the central nervous system in the mature animal. To study the early development of the murine central nervous system we have sought to isolate growth factor receptors from the neuroepithelium of the neural tube of 10-day-old mouse embryos. Because many grow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reid HH,Wilks AF,Bernard O

更新日期：1990-02-01 00:00:00

abstract：:Chromosome segregation and mitotic exit are initiated by the 1.2-MDa ubiquitin ligase APC/C (anaphase-promoting complex/cyclosome) and its coactivator CDC20 (cell division cycle 20). To avoid chromosome missegregation, APC/C(CDC20) activation is tightly controlled. CDC20 only associates with APC/C in mitosis when APC/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiao R,Weissmann F,Yamaguchi M,Brown NG,VanderLinden R,Imre R,Jarvis MA,Brunner MR,Davidson IF,Litos G,Haselbach D,Mechtler K,Stark H,Schulman BA,Peters JM

更新日期：2016-05-10 00:00:00

abstract：:Transmembrane 16A (TMEM16A, anoctamin1), 1 of 10 TMEM16 family proteins, is a Cl- channel activated by intracellular Ca2+ and membrane voltage. This channel is also regulated by the membrane phospholipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]. We find that two splice variants of TMEM16A show different sensi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ko W,Jung SR,Kim KW,Yeon JH,Park CG,Nam JH,Hille B,Suh BC

更新日期：2020-12-01 00:00:00

abstract：:Gene III protein of bacteriophage f1 is inserted into the host cell membrane where it is assembled into phage particles. A truncated form of gene III protein, encoded by a recombinant plasmid and lacking the carboxyl terminus, does not remain in the membrane but instead appears to slip through it. Fusion of a hydropho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boeke JD,Model P

更新日期：1982-09-01 00:00:00

abstract：:Differentiation-inducing factor 1 [DIF-1; 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)-hexan-1-one] induces stalk cell differentiation during Dictyostelium development. It is present as a gradient in the multicellular slug, its lowest concentration being in the anterior. Here we demonstrate the existence of a locali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kay RR,Large S,Traynor D,Nayler O

更新日期：1993-01-15 00:00:00

abstract：:The North Atlantic northern right whale (Eubalaena glacialis) is considered the most endangered large whale species. Its population has recovered only slowly since the cessation of commercial whaling and numbers about 300 individuals. We applied mark-recapture statistics to a catalog of photographically identified ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caswell H,Fujiwara M,Brault S

更新日期：1999-03-16 00:00:00

abstract：:We have generated a transgenic mouse line by microinjection of a chimeric DNA fragment (KER-CAT) containing a hair-specific, murine ultra-high-sulfur keratin promoter (KER) fused to the coding region of the bacterial chloramphenicol acetyltransferase (CAT) gene. A 671-base pair (bp) stretch of the 5' promoter region w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McNab AR,Andrus P,Wagner TE,Buhl AE,Waldon DJ,Kawabe TT,Rea TJ,Groppi V,Vogeli G

更新日期：1990-09-01 00:00:00

abstract：:Osteonectin is a prominent noncollagenous protein of developing bone. A 2150-base-pair cDNA coding for osteonectin, isolated from a bovine bone cell lambda gt11 expression library, was sequenced and identified by comparison with protein sequence data. The nucleotide sequence predicts that osteonectin contains 304 amin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bolander ME,Young MF,Fisher LW,Yamada Y,Termine JD

更新日期：1988-05-01 00:00:00

abstract：:The recent development of HIV-1 lentiviral vectors is especially useful for gene transfer because they achieve efficient integration into nondividing cell genomes and successful long-term expression of the transgene. These attributes make the vector useful for gene delivery, mutagenesis, and other applications in mamm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai Z,Han I,Park M,Brady RO

更新日期：2002-03-19 00:00:00

abstract：:N-Acetylaspartylglutamate, an endogenous brain peptide that binds with high affinity to a subpopulation of glutamate-binding sites in rat brain, is excitatory on rat piriform cortex pyramidal cells studied in a perfused brain slice. Both the monosynaptic excitation of the pyramidal cells elicited by stimulation of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ffrench-Mullen JM,Koller K,Zaczek R,Coyle JT,Hori N,Carpenter DO

更新日期：1985-06-01 00:00:00

abstract：:The causes of major and rapid transitions observed in biological macroevolution as well as in the evolution of social systems are a subject of much debate. Here we identify the proximate causes of crashes and recoveries that arise dynamically in a model system in which populations of (molecular) species coevolve with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jain S,Krishna S

更新日期：2002-02-19 00:00:00

abstract：:Fish in schooling formations navigate complex flow fields replete with mechanical energy in the vortex wakes of their companions. Their schooling behavior has been associated with evolutionary advantages including energy savings, yet the underlying physical mechanisms remain unknown. We show that fish can improve thei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Verma S,Novati G,Koumoutsakos P

更新日期：2018-06-05 00:00:00