Abstract:
:Mutations at the mouse limb deformity (ld) locus result in defects of growth and patterning of the limb and kidney during embryonic development. The gene responsible for this phenotype is large and complex, with the capacity to generate a number of alternatively spliced messenger RNA transcripts encoding nuclear protein isoforms called "formins." We have made polyclonal antibodies to specific formin peptides and have confirmed the authenticity of the antibodies' reactivity, using cell lines derived from mice with molecularly defined mutations at the ld locus. In addition, we have used these antibodies to detect and characterize polypeptides encoded by both wild-type and mutant ld alleles. In so doing, we show that a formin isoform (i) is modified by posttranslational phosphorylation at serine and threonine residues and (ii) when present in a crude nuclear extract, is retained by DNA-cellulose.
journal_name
Proc Natl Acad Sci U S Aauthors
Vogt TF,Jackson-Grusby L,Rush J,Leder Pdoi
10.1073/pnas.90.12.5554subject
Has Abstractpub_date
1993-06-15 00:00:00pages
5554-8issue
12eissn
0027-8424issn
1091-6490journal_volume
90pub_type
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