Differential amplification of specific areas of phage T4 genome as revealed by hybridization to cloned genetic segments.

abstract：:Splenic lymphocytes from a Lewis rat, immunized with purified estradiol-receptor complex of calf uterine nuclei, were fused with cells of three different mouse myeloma lines (P3-X63-Ag8, P3-NSI/I-Ag4-1, and Sp2/0-Ag14) to yield hybridoma cultures, 9% of which produced antibodies to the receptor protein (estrophilin). ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene GL,Fitch FW,Jensen EV

更新日期：1980-01-01 00:00:00

abstract：:Swarming bacteria move in multicellular groups and exhibit adaptive resistance to multiple antibiotics. Analysis of this phenomenon has revealed the protective power of high cell densities to withstand exposure to otherwise lethal antibiotic concentrations. We find that high densities promote bacterial survival, even ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler MT,Wang Q,Harshey RM

更新日期：2010-02-23 00:00:00

abstract：:The LSD1-CoREST histone demethylase complex is required to repress neuronal genes in nonneuronal tissues. Here we show that sumoylation of Braf35, one of the subunits of the complex, is required to maintain full repression of neuron-specific genes and for occupancy of the LSD1-CoREST complex at its gene targets. Inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ceballos-Chávez M,Rivero S,García-Gutiérrez P,Rodríguez-Paredes M,García-Domínguez M,Bhattacharya S,Reyes JC

更新日期：2012-05-22 00:00:00

abstract：:The transforming growth factor-beta (TGF-beta) superfamily of growth factors and cytokines has been implicated in a variety of physiological and developmental processes within the cardiovascular system. Smad proteins are a recently described family of intracellular signaling proteins that transduce signals in response...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Topper JN,DiChiara MR,Brown JD,Williams AJ,Falb D,Collins T,Gimbrone MA Jr

更新日期：1998-08-04 00:00:00

abstract：:Low phosphate (Pi) availability is one of the major constraints for plant productivity in natural and agricultural ecosystems. Plants have evolved a myriad of developmental and biochemical mechanisms to increase internal Pi uptake and utilization efficiency. One important biochemical pathway leading to an increase in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cruz-Ramírez A,Oropeza-Aburto A,Razo-Hernández F,Ramírez-Chávez E,Herrera-Estrella L

更新日期：2006-04-25 00:00:00

abstract：:Two additional peptides with inhibin-like activity have been isolated from human seminal plasma. One consists of 52 amino acids and the other, 92 amino acids. They are designated alpha-inhibin-52 and alpha-inhibin-92. Sequence analyses show that the NH2-terminal 31 amino acids of alpha-inhibin-52 are identical to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li CH,Hammonds RG Jr,Ramasharma K,Chung D

更新日期：1985-06-01 00:00:00

abstract：:H-NS and Lsr2 are nucleoid-associated proteins from Gram-negative bacteria and Mycobacteria, respectively, that play an important role in the silencing of horizontally acquired foreign DNA that is more AT-rich than the resident genome. Despite the fact that Lsr2 and H-NS proteins are dissimilar in sequence and structu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon BR,Li Y,Cote A,Weirauch MT,Ding P,Hughes TR,Navarre WW,Xia B,Liu J

更新日期：2011-06-28 00:00:00

abstract：:cDNA clones specific for the light subunit of porcine calpain I have been isolated from a porcine kidney cDNA library. The complete primary structure of the light subunit has been revealed by nucleotide sequence analysis of the cDNA clones isolated and amino acid sequence analysis of peptides isolated from the purifie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakihama T,Kakidani H,Zenita K,Yumoto N,Kikuchi T,Sasaki T,Kannagi R,Nakanishi S,Ohmori M,Takio K

更新日期：1985-09-01 00:00:00

abstract：:Integral membrane proteins that form water-filled channels through membranes often exist as aggregates of similar or identical subunits spanning the membrane. It has been suggested that the insertion into the membrane of the channel-forming domains of the subunits may impart unusual structural features to the membrane...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maher PA,Singer SJ

更新日期：1985-02-01 00:00:00

abstract：:Antisense oligodeoxyribonucleotides targeted to the epidermal growth factor (EGF) receptor were encapsulated into liposomes linked to folate via a polyethylene glycol spacer (folate-PEG-liposomes) and efficiently delivered into cultured KB cells via folate receptor-mediated endocytosis. The oligonucleotides were a pho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang S,Lee RJ,Cauchon G,Gorenstein DG,Low PS

更新日期：1995-04-11 00:00:00

abstract：:The single-stranded mitochondrial DNA (mtDNA) displacement-loop initiation sequence (7S mtDNA) is hydrogen-bonded at the origin of replication in animal cell mtDNA. Analysis of 7S mtDNA from several cell sources indicates that this initiation sequence exists as a family of fragments of relatively discrete lengths. mtD...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gillum AM,Clayton DA

更新日期：1978-02-01 00:00:00

abstract：:The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10(-6) M, requires no more than 1 min of preincubation for the maximal effect, and decays linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoover RL,Karnovsky MJ,Austen KF,Corey EJ,Lewis RA

更新日期：1984-04-01 00:00:00

abstract：:Retinoblastoma-related (RBR) genes inhibit the cell cycle primarily by repressing adenovirus E2 promoter binding factor (E2F) transcription factors, which drive the expression of numerous genes required for DNA synthesis and cell cycle progression. The RBR-E2F pathway is conserved in plants, but cereals such as maize ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sabelli PA,Hoerster G,Lizarraga LE,Brown SW,Gordon-Kamm WJ,Larkins BA

更新日期：2009-03-10 00:00:00

abstract：:Fine finger and hand movements in humans, monkeys, and rats are under the direct control of the corticospinal tract (CST). CST lesions lead to severe, long-term deficits of precision movements. We transected completely both CSTs in adult rats and treated the animals for 2 weeks with an antibody that neutralized the ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raineteau O,Fouad K,Noth P,Thallmair M,Schwab ME

更新日期：2001-06-05 00:00:00

abstract：:The members of the cytoplasmic 70-kDa heat shock protein family are involved in appropriate folding and trafficking of newly synthesized proteins in the cell. Hsc70, which is expressed constitutively, and Hsp70, the expression of which is stress- and heat shock-induced, are often considered to have similar cellular fu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldfarb SB,Kashlan OB,Watkins JN,Suaud L,Yan W,Kleyman TR,Rubenstein RC

更新日期：2006-04-11 00:00:00

abstract：:The adaptations in the freshwater turtle that permit survival despite prolonged loss of mitochondrial ATP generation were investigated by comparing the bioenergetics of turtle brain slices with rat brain slices. Aerobic turtle brain shows no significant difference in basal levels of total ATP generation compared to ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robin ED,Lewiston N,Newman A,Simon LM,Theodore J

更新日期：1979-08-01 00:00:00

abstract：:Behavioral sensitization of the gill-withdrawal reflex of Aplysia is caused by presynaptic facilitation at the synapses of the mechanoreceptor sensory neurons of the reflex onto the motor neurons and interneurons. The presynaptic facilitation has been shown to be simulated by serotonin (the putative presynaptic facili...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein M,Kandel ER

更新日期：1980-11-01 00:00:00

abstract：:By using a combination of genetic, pharmacological, and anatomical approaches, we show that the melanocortin 4 receptor (MC4R), implicated in the control of food intake and energy expenditure, also modulates erectile function and sexual behavior. Evidence supporting this notion is based on several findings: (i) a high...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van der Ploeg LH,Martin WJ,Howard AD,Nargund RP,Austin CP,Guan X,Drisko J,Cashen D,Sebhat I,Patchett AA,Figueroa DJ,DiLella AG,Connolly BM,Weinberg DH,Tan CP,Palyha OC,Pong SS,MacNeil T,Rosenblum C,Vongs A,Tang R

更新日期：2002-08-20 00:00:00

abstract：:Elevated eukaryotic initiation factor 4E (eIF4E) levels frequently occur in a variety of human cancers. Overexpression of eIF4E promotes cellular transformation by selectively increasing the translation of proliferative and prosurvival mRNAs. These mRNAs possess highly structured 5'-UTRs that impede ribosome recruitme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feoktistova K,Tuvshintogs E,Do A,Fraser CS

更新日期：2013-08-13 00:00:00

abstract：:A complementarity-determining region (CDR) of the mouse monoclonal antibody (mAb) F58 was constructed with specificity to a neutralization-inducing region of human immunodeficiency virus type 1 (HIV-1). The mAb has its major reactivity to the amino acid sequence I--GPGRA in the V3 viral envelope region. All CDRs inclu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levi M,Sällberg M,Rudén U,Herlyn D,Maruyama H,Wigzell H,Marks J,Wahren B

更新日期：1993-05-15 00:00:00

abstract：:The 33-amino-acid ankyrin motif comprises a beta-turn followed by two anti-parallel alpha-helices and a loop and tandem arrays of the motif pack in a linear fashion to produce elongated structures characterized by short-range interactions. In this article we use site-directed mutagenesis to investigate the kinetic unf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Werbeck ND,Rowling PJ,Chellamuthu VR,Itzhaki LS

更新日期：2008-07-22 00:00:00

abstract：:Genetic screens have been performed to identify mutants with altered auxin homeostasis in Arabidopsis. A tagged allele of the auxin-overproducing mutant sur2 was identified within a transposon mutagenized population. The SUR2 gene was cloned and shown to encode the CYP83B1 protein, which belongs to the large family of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barlier I,Kowalczyk M,Marchant A,Ljung K,Bhalerao R,Bennett M,Sandberg G,Bellini C

更新日期：2000-12-19 00:00:00

abstract：:Synthetic oligonucleotide probes can be easily obtained and used, in contrast to cDNA cloning to develop probes, and thus the present study was carried out to determine whether such probes could also be useful for in situ hybridization. A 24-base synthetic oligonucleotide complementary to part of the alpha-melanocyte-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis ME,Sherman TG,Burke S,Akil H,Davis LG,Arentzen R,Watson SJ

更新日期：1986-08-01 00:00:00

abstract：:TP53 (p53) is a tumor suppressor whose functions are lost or altered in most malignancies. p53 homozygous knockout (p53-/-) mice uniformly die of spontaneous malignancy, typically T-cell lymphoma. RALBP1 (RLIP76, Rlip) is a stress-protective, mercapturic acid pathway transporter protein that also functions as a Ral ef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Awasthi S,Tompkins J,Singhal J,Riggs AD,Yadav S,Wu X,Singh S,Warden C,Liu Z,Wang J,Slavin TP,Weitzel JN,Yuan YC,Awasthi M,Srivastava SK,Awasthi YC,Singhal SS

更新日期：2018-04-10 00:00:00

abstract：:Border cell migration is a stereotyped migration occurring during the development of the Drosophila egg chamber. During this process, a cluster composed of six to eight follicle cells migrates between nurse cells toward the oocyte. Receptor tyrosine kinases (RTKs) are enriched at the leading edge of the follicle cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Assaker G,Ramel D,Wculek SK,González-Gaitán M,Emery G

更新日期：2010-12-28 00:00:00

abstract：:Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson LH,Mankovitz R,Baker RM,Till JE,Siminovitch L,Whitmore GF

更新日期：1970-06-01 00:00:00

abstract：:We explore the interactions of CYT-19, a DExD/H-box protein that functions in folding of group I RNAs, with a well characterized misfolded species of the Tetrahymena ribozyme. Consistent with its function, CYT-19 accelerates refolding of the misfolded RNA to its native state. Unexpectedly, CYT-19 performs another reac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tijerina P,Bhaskaran H,Russell R

更新日期：2006-11-07 00:00:00

abstract：:B-DNA becomes unstable under superhelical stress and is able to adopt a wide range of alternative conformations including strand-separated DNA and Z-DNA. Localized sequence-dependent structural transitions are important for the regulation of biological processes such as DNA replication and transcription. To directly p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oberstrass FC,Fernandes LE,Bryant Z

更新日期：2012-04-17 00:00:00

abstract：:Ghrelin is a newly discovered peptide and an endogenous ligand for growth hormone (GH) secretagogue (GHS) receptor. It has been shown to possess various central and peripheral effects, including GH secretion, food intake, and gastric and cardiac effects. Ghrelin and the GHS receptor are expressed also in pancreatic is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doi A,Shono T,Nishi M,Furuta H,Sasaki H,Nanjo K

更新日期：2006-01-24 00:00:00

abstract：:A Raleigh, NC, population of Drosophila melanogaster was sampled for the presence of enzyme null alleles at 25 loci. No nulls were found at any of five X-linked loci. Nulls were recovered at 13 of 20 autosomal loci; the weighted mean frequency for all 20 autosomal loci was estimated to be 0.0025. A consideration of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voelker RA,Langley CH,Brown AJ,Ohnishi S,Dickson B,Montgomery E,Smith SC

更新日期：1980-02-01 00:00:00