Control of neuronal differentiation by sumoylation of BRAF35, a subunit of the LSD1-CoREST histone demethylase complex.

abstract：:15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gonnella NC,Birdseye TR,Nee M,Roberts JD

更新日期：1982-08-01 00:00:00

abstract：:We have used oligonucleotide-directed mutagenesis to make a specific change in the beta-lactamase (EC 3.5.2.6) (ampicillin resistance) gene of the plasmid pBR322. Evidence suggests that the active site for this enzyme may include a serine-threonine dyad (residues 70 and 71). By priming in vitro DNA synthesis with a ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dalbadie-McFarland G,Cohen LW,Riggs AD,Morin C,Itakura K,Richards JH

更新日期：1982-11-01 00:00:00

abstract：:Gel-forming mucins, the primary macromolecular components of airway mucus, facilitate airway clearance by mucociliary transport. In cystic fibrosis (CF) altered mucus properties impair mucociliary transport. Airways primarily secrete two closely related gel-forming mucins, MUC5B and MUC5AC. However, their morphologic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ostedgaard LS,Moninger TO,McMenimen JD,Sawin NM,Parker CP,Thornell IM,Powers LS,Gansemer ND,Bouzek DC,Cook DP,Meyerholz DK,Abou Alaiwa MH,Stoltz DA,Welsh MJ

更新日期：2017-06-27 00:00:00

abstract：:Cigarette smoking causes emphysema, a fatal disease involving extensive structural and functional damage of the lung. Using a guinea pig model and human lung cells, we show that oxidant(s) present in tobacco smoke not only cause direct oxidative damage of lung proteins, contributing to the major share of lung injury, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gupta I,Ganguly S,Rozanas CR,Stuehr DJ,Panda K

更新日期：2016-07-19 00:00:00

abstract：:Egg activation is the process by which a mature oocyte becomes capable of supporting embryo development. In vertebrates and echinoderms, activation is induced by fertilization. Molecules introduced into the egg by the sperm trigger progressive release of intracellular calcium stores in the oocyte. Calcium wave(s) spre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaneuchi T,Sartain CV,Takeo S,Horner VL,Buehner NA,Aigaki T,Wolfner MF

更新日期：2015-01-20 00:00:00

abstract：:Ciliary neurotrophic factor (CNTF) receptor controls a pathway supporting the differentiation and survival of a wide range of neural cell types during development and in adulthood. Cardiotrophin-like cytokine (CLC)-cytokine-like factor 1 (CLF) composite cytokine is a second ligand for the CNTF alpha-component receptor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rousseau F,Gauchat JF,McLeod JG,Chevalier S,Guillet C,Guilhot F,Cognet I,Froger J,Hahn AF,Knappskog PM,Gascan H,Boman H

更新日期：2006-06-27 00:00:00

abstract：:A major challenge in research on executive control is to reveal its functional decomposition into underlying neural mechanisms. A typical assumption is that this decomposition occurs solely through anatomically based dissociations. Here we tested an alternative hypothesis that different cognitive control processes may...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braver TS,Paxton JL,Locke HS,Barch DM

更新日期：2009-05-05 00:00:00

abstract：:Dyskeratosis congenita is a premature aging syndrome characterized by muco-cutaneous features and a range of other abnormalities, including early greying, dental loss, osteoporosis, and malignancy. Dyskeratosis congenita cells age prematurely and have very short telomeres. Patients have mutations in genes that encode ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vulliamy T,Beswick R,Kirwan M,Marrone A,Digweed M,Walne A,Dokal I

更新日期：2008-06-10 00:00:00

abstract：:At each stage of infection, the major coat protein of coliphage M13 binds to the E. coli cytoplasmic membrane with its antigenic site exposed to the cell exterior [Wickner, W. (1975) Proc. Nat. Acad. Sci. USA 72, 4749-4753]. This antigenic site is now shown to be at the amino-terminus of the protein. The amino-terminu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wickner W

更新日期：1976-04-01 00:00:00

abstract：:Complex I functions as a redox-linked proton pump in the respiratory chains of mitochondria and bacteria, driven by the reduction of quinone (Q) by NADH. Remarkably, the distance between the Q reduction site and the most distant proton channels extends nearly 200 Å. To elucidate the molecular origin of this long-range...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma V,Belevich G,Gamiz-Hernandez AP,Róg T,Vattulainen I,Verkhovskaya ML,Wikström M,Hummer G,Kaila VR

更新日期：2015-09-15 00:00:00

abstract：:Sequences of each of the four framework segments FR1, FR2, FR3, and FR4 of the variable regions (V-regions) of light and heavy chains of immunoglobulins were grouped into sets with identical sequences. Sets contained from 1 to 18 members. When each V-region was traced from one FR to the next, it was seen that members ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kabat EA,Wu TT,Bilofsky H

更新日期：1978-05-01 00:00:00

abstract：:The baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV, which is representative of the MNPV subtype in which the virions may contain many nucleocapsids within a single viral envelope) encodes a protein, v-ubi, that has 76% identity with the eukaryotic protein ubiquitin. Transcriptional mapping indic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guarino LA

更新日期：1990-01-01 00:00:00

abstract：:Integrated hepatitis B virus (HBV) DNA is almost invariably found in hepatocellular carcinomas (HCC) which develop in HBV carriers. Integrated HBV DNAs from two single-integration HCCs (C3 and C4) have been cloned, and the cellular integration sites have been analyzed. Integrated HBV DNA of C3 is present in chromosome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hino O,Shows TB,Rogler CE

更新日期：1986-11-01 00:00:00

abstract：:The lamB protein, the receptor for phage lambda, was purified from the outer membrane of Escherichia coli K-12 by extraction with Triton X-100 and EDTA, chromatography on DEAE-Sephacel in Triton X-100, exchange of Triton for cholate by gel filtration, and chromatography on Sephacryl S-200 in cholate, NaCl, and EDTA. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luckey M,Nikaido H

更新日期：1980-01-01 00:00:00

abstract：:Preterm birth (PTB) complications are the leading cause of long-term morbidity and mortality in children. By using whole blood samples, we integrated whole-genome sequencing (WGS), RNA sequencing (RNA-seq), and DNA methylation data for 270 PTB and 521 control families. We analyzed this combined dataset to identify gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knijnenburg TA,Vockley JG,Chambwe N,Gibbs DL,Humphries C,Huddleston KC,Klein E,Kothiyal P,Tasseff R,Dhankani V,Bodian DL,Wong WSW,Glusman G,Mauldin DE,Miller M,Slagel J,Elasady S,Roach JC,Kramer R,Leinonen K,Linth

更新日期：2019-03-19 00:00:00

abstract：:The cellulose synthesizing terminal complex consisting of subunits A, B, C, and D in Acetobacter xylinum spans the outer and inner cell membranes to synthesize and extrude glucan chains, which are assembled into subelementary fibrils and further into a ribbon. We determined the structures of subunit D (AxCeSD/AxBcsD) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu SQ,Gao YG,Tajima K,Sunagawa N,Zhou Y,Kawano S,Fujiwara T,Yoda T,Shimura D,Satoh Y,Munekata M,Tanaka I,Yao M

更新日期：2010-10-19 00:00:00

abstract：:Escherichia coli O157:H7, a toxin-producing food and waterborne bacterial pathogen, has been linked to large outbreaks of gastrointestinal illness for more than two decades. E. coli O157 causes a wide range of clinical illness that varies by outbreak, although factors that contribute to variation in disease severity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manning SD,Motiwala AS,Springman AC,Qi W,Lacher DW,Ouellette LM,Mladonicky JM,Somsel P,Rudrik JT,Dietrich SE,Zhang W,Swaminathan B,Alland D,Whittam TS

更新日期：2008-03-25 00:00:00

abstract：:Gene transfer into early embryos is a powerful methodology for unraveling the molecular bases of developmental processes. One can attempt to minimize widespread effects of an exogenous gene by using tissue- or region-specific promoters in the few instances where they are available. We have developed a method that bypa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fekete DM,Cepko CL

更新日期：1993-03-15 00:00:00

abstract：:Biotin plays an essential role in growth of mycobacteria. Synthesis of the cofactor is essential for Mycobacterium tuberculosis to establish and maintain chronic infections in a murine model of tuberculosis. Although the late steps of mycobacterial biotin synthesis, assembly of the heterocyclic rings, are thought to f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Z,Cronan JE

更新日期：2020-09-22 00:00:00

abstract：:A constitutively active form of mitogen-activated protein kinase kinase (MEK1) was synthesized under control of a zinc-inducible promoter in NIH 3T3 fibroblasts. Zinc treatment of serum-starved cells activated extracellular signal-regulated protein kinases (ERKs) and induced expression of cyclin D1. Newly synthesized ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng M,Sexl V,Sherr CJ,Roussel MF

更新日期：1998-02-03 00:00:00

abstract：:Yeast to directly convert cellulose and, especially, the microcrystalline cellulose into bioethanol, was engineered through display of minicellulosomes on the cell surface of Saccharomyces cerevisiae. The construction and cell surface attachment of cellulosomes were accomplished with two individual miniscaffoldins to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan LH,Zhang ZJ,Yu XY,Xue YX,Tan TW

更新日期：2012-08-14 00:00:00

abstract：:We present two methods to probe the energy landscape and motions of proteins in the context of molecular dynamics simulations of the helix-forming S-peptide of RNase A and the RNase A-3'-UMP enzyme-product complex. The first method uses the generalized ergodic measure to compute the rate of conformational space sampli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Straub JE,Thirumalai D

更新日期：1993-02-01 00:00:00

abstract：:Resting- or baseline-state low-frequency (0.01-0.2 Hz) brain activity is observed in fMRI, EEG, and local field potential recordings. These fluctuations were found to be correlated across brain regions and are thought to reflect neuronal activity fluctuations between functionally connected areas of the brain. However,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krishnan GP,González OC,Bazhenov M

更新日期：2018-06-26 00:00:00

abstract：:We have investigated the folding dynamics of Thermus thermophilus cytochrome c(552) by time-resolved fluorescence energy transfer between the heme and each of seven site-specific fluorescent probes. We have found both an equilibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies. De...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada S,Bouley Ford ND,Keller GE,Ford WC,Gray HB,Winkler JR

更新日期：2013-01-29 00:00:00

abstract：:Genetic variation in the serotonin transporter gene (SLC6A4) is associated with vulnerability to affective disorders and pharmacotherapy efficacy. We recently identified sequence polymorphisms in the common marmoset SLC6A4 repeat region (AC/C/G and CT/T/C) associated with individual differences in anxiety-like trait, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santangelo AM,Sawiak SJ,Fryer T,Hong Y,Shiba Y,Clarke HF,Riss PJ,Ferrari V,Tait R,Suckling J,Aigbirhio FI,Roberts AC

更新日期：2019-07-16 00:00:00

abstract：:The response of nerve cells to synaptic inputs and the propagation of this activation is critically dependent on the cell-surface distribution of ion channels. In the hippocampus, Ca2+ influx through N-methyl-D-aspartate receptors (NMDAR) and/or voltage-dependent calcium channels on dendrites is thought to be critical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benke TA,Jones OT,Collingridge GL,Angelides KJ

更新日期：1993-08-15 00:00:00

abstract：:Investigations using the fruit fly Drosophila melanogaster have shown that the circadian clock gene period (Per) can influence behavioral responses to cocaine. Here we show that the mouse homologues of the Drosophila Per gene, mPer1 and mPer2, modulate cocaine sensitization and reward, two phenomena extensively studie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abarca C,Albrecht U,Spanagel R

更新日期：2002-06-25 00:00:00

abstract：:The accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and triggers the unfolded protein response (UPR). Failure to resolve ER stress leads to apoptotic cell death via a yet unclear mechanism. Here, we show that RNF183, a membrane-spanning RING finger protein, localizes to the ER and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Y,Li X,Jia J,Zhang Y,Li J,Zhu Z,Wang H,Tang J,Hu J

更新日期：2018-03-20 00:00:00

abstract：:After exposure of segments of rabbit taenia coli to the nonionic detergent Triton X-100, tension could be induced by increasing the [Ca2+] in the micromolar range. In the presence of a saturating [Ca2+], this preparation developed nearly 100% of the control tetanus tension recorded from the intact muscle prior to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon AR

更新日期：1978-07-01 00:00:00

abstract：:The density of benzodiazepine/gamma-aminobutyric acid receptor binding sites was lower in the midbrain of seizure-susceptible gerbils compared to control seizure-resistant gerbils. Binding of [3H]diazepam to high-affinity brain-specific sites in membrane homogenates of gerbil brain showed a 20-30% lower binding in mid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olsen RW,Wamsley JK,McCabe RT,Lee RJ,Lomax P

更新日期：1985-10-01 00:00:00