当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.

Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.

Abstract：

:The lamB protein, the receptor for phage lambda, was purified from the outer membrane of Escherichia coli K-12 by extraction with Triton X-100 and EDTA, chromatography on DEAE-Sephacel in Triton X-100, exchange of Triton for cholate by gel filtration, and chromatography on Sephacryl S-200 in cholate, NaCl, and EDTA. The purified protein appeared to exist as several oligomeric species. In an equilibrium retention assay with reconstituted vesicles containing phospholipids and lipopolysaccharide, the lamB protein conferred permeability for disaccharides. In a liposome swelling assay designed to measure rates of diffusion, the lamB protein conferred permeability to phospholipid liposomes for a variety of substrates. The rates obtained indicate the permeation facilitated by the lamB protein is specific, discriminating among substrates by both size and configuration. For example, maltose diffused into liposomes 40 times faster than sucrose, about 8 times faster than cellobiose, and about 12 times faster than maltoheptaose. The results suggest that the lamB protein forms a transmembrane channel containing a site (or sites) that loosely interacts with the solutes.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Luckey M,Nikaido H

doi

10.1073/pnas.77.1.167

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

167-71

issue

1

eissn

0027-8424

issn

1091-6490

journal_volume

77

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Processing of the 5' end of Escherichia coli 16S ribosomal RNA.

    abstract：:We have isolated and partially characterized an endonuclease involved in processing the 5' end of 16S rRNA of Escherichia coli. A mutant strain that is deficient in this enzyme accumulates a new precursor of 16S rRNA, named 16.3S rRNA. This rRNA has the 3' end of mature 16S rRNA but is about 60 nucleotides longer at t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.8.3598

    authors： Dahlberg AE,Dahlberg JE,Lund E,Tokimatsu H,Rabson AB,Calvert PC,Reynolds F,Zahalak M

    更新日期：1978-08-01 00:00:00

  • Epidermal growth factor stimulates tyrosine phosphorylation in the neonatal mouse: association of a M(r) 55,000 substrate with the receptor.

    abstract：:Administration of epidermal growth factor (EGF) to neonatal mice resulted in rapid tyrosine phosphorylation of a number of specific substrates in liver, kidney, lung, bladder, skin, and brain as detected by Western blot analysis of tissue homogenates with anti-phosphotyrosine antibodies. In the liver, three prominent ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.18.8477

    authors： Donaldson RW,Cohen S

    更新日期：1992-09-15 00:00:00

  • Specific inhibition of herpes simplex virus DNA polymerase by helical peptides corresponding to the subunit interface.

    abstract：:The herpes simplex virus DNA polymerase consists of two subunits--a catalytic subunit and an accessory subunit, UL42, that increases processivity. Mutations affecting the extreme C terminus of the catalytic subunit specifically disrupt subunit interactions and ablate virus replication, suggesting that new antiviral dr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.5.1456

    authors： Digard P,Williams KP,Hensley P,Brooks IS,Dahl CE,Coen DM

    更新日期：1995-02-28 00:00:00

  • Binding between the Niemann-Pick C1 protein and a photoactivatable cholesterol analog requires a functional sterol-sensing domain.

    abstract：:Niemann-Pick type C (NPC) 1 protein plays important roles in moving cholesterol and other lipids out of late endosomes by means of vesicular trafficking, but it is not known whether NPC1 directly interacts with cholesterol. We performed photoaffinity labeling of intact cells expressing fluorescent protein (FP)-tagged ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0405255101

    authors： Ohgami N,Ko DC,Thomas M,Scott MP,Chang CC,Chang TY

    更新日期：2004-08-24 00:00:00

  • The role of members of the pertussis toxin-sensitive family of G proteins in coupling receptors to the activation of the G protein-gated inwardly rectifying potassium channel.

    abstract：:Inwardly rectifying potassium (K(+)) channels gated by G proteins (Kir3.x family) are widely distributed in neuronal, atrial, and endocrine tissues and play key roles in generating late inhibitory postsynaptic potentials, slowing the heart rate and modulating hormone release. They are directly activated by G(betagamma...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.080572297

    authors： Leaney JL,Tinker A

    更新日期：2000-05-09 00:00:00

  • Serotonin 2B receptor is required for heart development.

    abstract：:Several lines of evidence suggest that the serotonin (5-hydroxytryptamine, 5-HT) regulates cardiovascular functions during embryogenesis and adulthood. 5-HT binds to numerous cognate receptors to initiate its biological effects. However, none of the 5-HT receptor disruptions in mice have yet resulted in embryonic defe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.17.9508

    authors： Nebigil CG,Choi DS,Dierich A,Hickel P,Le Meur M,Messaddeq N,Launay JM,Maroteaux L

    更新日期：2000-08-15 00:00:00

  • Interleukin 2 acts as an adjuvant to increase the potency of inactivated rabies virus vaccine.

    abstract：:Interleukin 2 (IL-2) occupies a central position in the cascade of events involved in the immune response. We were interested in determining whether IL-2 could function as an adjuvant to vaccination, to increase the immune response to vaccine immunogens. Using the National Institutes of Health test for rabies vaccine ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.11.4240

    authors： Nunberg JH,Doyle MV,York SM,York CJ

    更新日期：1989-06-01 00:00:00

  • Molecular cloning of the flavin-containing monooxygenase (form II) cDNA from adult human liver.

    abstract：:Complementary DNA (cDNA) clones encoding the adult human liver flavin-containing monooxygenase (FMO; dimethylaniline N-oxidase, EC 1.14.13.8) were isolated from lambda gt10 and lambda gt11 libraries. The cDNA libraries were screened with three synthetic 36-mer oligonucleotide probes derived from the nucleic acid seque...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.5.1685

    authors： Lomri N,Gu Q,Cashman JR

    更新日期：1992-03-01 00:00:00

  • Association of a common complement receptor 2 haplotype with increased risk of systemic lupus erythematosus.

    abstract：:A genomic region on distal mouse chromosome 1 and its syntenic human counterpart 1q23-42 show strong evidence of harboring lupus susceptibility genes. We found evidence of linkage at 1q32.2 in a targeted genome scan of 1q21-43 in 126 lupus multiplex families containing 151 affected sibpairs (nonparametric linkage scor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0609101104

    authors： Wu H,Boackle SA,Hanvivadhanakul P,Ulgiati D,Grossman JM,Lee Y,Shen N,Abraham LJ,Mercer TR,Park E,Hebert LA,Rovin BH,Birmingham DJ,Chang DM,Chen CJ,McCurdy D,Badsha HM,Thong BY,Chng HH,Arnett FC,Wallace DJ,Yu CY

    更新日期：2007-03-06 00:00:00

  • Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein.

    abstract：:Multiple sclerosis is a chronic disease of the central nervous system (CNS) characterized by inflammation, demyelination, and axonal loss. The immunopathogenesis of demyelination in multiple sclerosis involves an autoantibody response to myelin oligodendrocyte glycoprotein (MOG), a type I transmembrane protein located...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1133443100

    authors： Breithaupt C,Schubart A,Zander H,Skerra A,Huber R,Linington C,Jacob U

    更新日期：2003-08-05 00:00:00

  • Variable genetic architectures produce virtually identical molecules in bacterial symbionts of fungus-growing ants.

    abstract：:Small molecules produced by Actinobacteria have played a prominent role in both drug discovery and organic chemistry. As part of a larger study of the actinobacterial symbionts of fungus-growing ants, we discovered a small family of three previously unreported piperazic acid-containing cyclic depsipeptides, gerumycins...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1515348112

    authors： Sit CS,Ruzzini AC,Van Arnam EB,Ramadhar TR,Currie CR,Clardy J

    更新日期：2015-10-27 00:00:00

  • Thymosin corrects the abnormal DNA synthetic response of NZB mouse thymocytes.

    abstract：:New Zealand Black (NZB) mice develop after 16 weeks of age an autoimmune and lymphoproliferative disease which is a model for systemic lupus erythematosus and lymphoid malignancy in humans. At this age, the mice manifest a progressive decline in T lymphocyte (thymus-derived lymphocyte) functions and serum thymosin lev...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.7.2637

    authors： Dauphinee MJ,Talal N,Goldstein AL,White A

    更新日期：1974-07-01 00:00:00

  • Dual roles of modulatory calcineurin-interacting protein 1 in cardiac hypertrophy.

    abstract：:The calcium/calmodulin-dependent protein phosphatase calcineurin stimulates cardiac hypertrophy in response to numerous stimuli. Calcineurin activity is suppressed by association with modulatory calcineurin-interacting protein (MCIP)1DSCR1, which is up-regulated by calcineurin signaling and has been proposed to functi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0237225100

    authors： Vega RB,Rothermel BA,Weinheimer CJ,Kovacs A,Naseem RH,Bassel-Duby R,Williams RS,Olson EN

    更新日期：2003-01-21 00:00:00

  • Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration.

    abstract：:Neuropathy target esterase (NTE) is a neuronal membrane protein originally identified for its property to be modified by organo-phosphates (OPs), which in humans cause neuropathy characterized by axonal degeneration. Drosophila mutants for the homolog gene of NTE, swisscheese (sws), indicated a possible involvement of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0401030101

    authors： Akassoglou K,Malester B,Xu J,Tessarollo L,Rosenbluth J,Chao MV

    更新日期：2004-04-06 00:00:00

  • Reduced vacuolar β-1,3-glucan synthesis affects carbohydrate metabolism as well as plastid homeostasis and structure in Phaeodactylum tricornutum.

    abstract：:The β-1,3-glucan chrysolaminarin is the main storage polysaccharide of diatoms. In contrast to plants and green algae, diatoms and most other algal groups do not accumulate storage polysaccharides in their plastids. The diatom Phaeodactylum tricornutum possesses only a single gene encoding a putative β-1,3-glucan synt...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1719274115

    authors： Huang W,Haferkamp I,Lepetit B,Molchanova M,Hou S,Jeblick W,Río Bártulos C,Kroth PG

    更新日期：2018-05-01 00:00:00

  • Mechanism of dexamethasone inhibition of plasminogen activator in rat hepatoma cells.

    abstract：:Glucocorticoids rapidly and completely inhibit intracellular plasminogen activator activity in rat hepatoma cells, as assayed by the solubilization of 125I-labeled fibrin. Experiments in which extracts of dexamethasone-treated cells are mixed with extracts of control cells demonstrate an inhibitor of protease activity...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.12.6130

    authors： Seifert SC,Gelehrter TD

    更新日期：1978-12-01 00:00:00

  • The serum response element can mediate induction of c-fos by growth hormone.

    abstract：:The c-fos protooncogene is transcriptionally activated by a wide variety of agents including serum, growth factors, and phorbol esters. This induction is rapid and transient and is mediated through a number of identified promoter elements. Growth hormone (GH) is also known to induce transcription of c-fos in a variety...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.14.6721

    authors： Meyer DJ,Stephenson EW,Johnson L,Cochran BH,Schwartz J

    更新日期：1993-07-15 00:00:00

  • Chronic disability trends in elderly United States populations: 1982-1994.

    abstract：:Statistically significant declines in chronic disability prevalence rates were observed in the elderly United States population between the 1982 and 1989 National Long Term Care Surveys (NLTCS). The 1994 NLTCS was used to investigate whether disability rate declines continued to 1994. The 1982, 1984, 1989, and 1994 NL...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.6.2593

    authors： Manton KG,Corder L,Stallard E

    更新日期：1997-03-18 00:00:00

  • Chloroplast Omp85 proteins change orientation during evolution.

    abstract：:The majority of outer membrane proteins (OMPs) from gram-negative bacteria and many of mitochondria and chloroplasts are β-barrels. Insertion and assembly of these proteins are catalyzed by the Omp85 protein family in a seemingly conserved process. All members of this family exhibit a characteristic N-terminal polypep...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1108626108

    authors： Sommer MS,Daum B,Gross LE,Weis BL,Mirus O,Abram L,Maier UG,Kühlbrandt W,Schleiff E

    更新日期：2011-08-16 00:00:00

  • Human genes involved in cholesterol metabolism: chromosomal mapping of the loci for the low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl-coenzyme A reductase with cDNA probes.

    abstract：:Cellular cholesterol metabolism is regulated primarily through the coordinate expression of two proteins, the low density lipoprotein (LDL) receptor and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (EC 1.1.1.34). We have used cDNA probes for the human genes encoding these proteins to determine the precise...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.24.8567

    authors： Lindgren V,Luskey KL,Russell DW,Francke U

    更新日期：1985-12-01 00:00:00

  • Targeting transcriptional regulation of SARS-CoV-2 entry factors ACE2 and TMPRSS2.

    abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19, employs two key host proteins to gain entry and replicate within cells, angiotensin-converting enzyme 2 (ACE2) and the cell surface transmembrane protease serine 2 (TMPRSS2). TMPRSS2 was first characterized as an androgen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2021450118

    authors： Qiao Y,Wang XM,Mannan R,Pitchiaya S,Zhang Y,Wotring JW,Xiao L,Robinson DR,Wu YM,Tien JC,Cao X,Simko SA,Apel IJ,Bawa P,Kregel S,Narayanan SP,Raskind G,Ellison SJ,Parolia A,Zelenka-Wang S,McMurry L,Su F,Wang R,

    更新日期：2020-12-11 00:00:00

  • Function of the bacterial TATAAT -10 element as single-stranded DNA during RNA polymerase isomerization.

    abstract：:The bacterial TATAAT -10 region sequence was the first promoter element to be identified, but how it functions is still not clear. Because the duplex element is melted during initiation, the effects of substitutions were studied in both single-and double-strand contexts. Band-shift results were particularly unexpected...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.161085798

    authors： Fenton MS,Gralla JD

    更新日期：2001-07-31 00:00:00

  • Modular chemical mechanism predicts spatiotemporal dynamics of initiation in the complex network of hemostasis.

    abstract：:This article demonstrates that a simple chemical model system, built by using a modular approach, may be used to predict the spatiotemporal dynamics of initiation of blood clotting in the complex network of hemostasis. Microfluidics was used to create in vitro environments that expose both the complex network and the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0605560103

    authors： Kastrup CJ,Runyon MK,Shen F,Ismagilov RF

    更新日期：2006-10-24 00:00:00

  • Declining survival probability threatens the North Atlantic right whale.

    abstract：:The North Atlantic northern right whale (Eubalaena glacialis) is considered the most endangered large whale species. Its population has recovered only slowly since the cessation of commercial whaling and numbers about 300 individuals. We applied mark-recapture statistics to a catalog of photographically identified ind...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.6.3308

    authors： Caswell H,Fujiwara M,Brault S

    更新日期：1999-03-16 00:00:00

  • Sog/Chordin is required for ventral-to-dorsal Dpp/BMP transport and head formation in a short germ insect.

    abstract：:Bone morphogenetic protein (BMP) signaling plays a major role in dorsoventral patterning in vertebrates and in Drosophila. Remarkably, in Tribolium, a beetle with an ancestral type of insect development, early BMP/dpp exhibits differential expression along the anteroposterior axis. However, the BMP/Dpp inhibitor Sog/c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0605154103

    authors： van der Zee M,Stockhammer O,von Levetzow C,Nunes da Fonseca R,Roth S

    更新日期：2006-10-31 00:00:00

  • RNA hairpins in noncoding regions of human brain and Caenorhabditis elegans mRNA are edited by adenosine deaminases that act on RNA.

    abstract：:Adenosine deaminases that act on RNA (ADARs) constitute a family of RNA-editing enzymes that convert adenosine to inosine within double-stranded regions of RNA. We previously developed a method to identify inosine-containing RNAs and used it to identify five ADAR substrates in Caenorhabditis elegans. Here we use the s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.112704299

    authors： Morse DP,Aruscavage PJ,Bass BL

    更新日期：2002-06-11 00:00:00

  • Inhibition of Wnt/beta-catenin/CREB binding protein (CBP) signaling reverses pulmonary fibrosis.

    abstract：:Idiopathic pulmonary fibrosis (IPF)/usual interstitial pneumonia is a ravaging condition of progressive lung scarring and destruction. Anti-inflammatory therapies including corticosteroids have limited efficacy in this ultimately fatal disorder. An important unmet need is to identify new agents that interact with key ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1001520107

    authors： Henderson WR Jr,Chi EY,Ye X,Nguyen C,Tien YT,Zhou B,Borok Z,Knight DA,Kahn M

    更新日期：2010-08-10 00:00:00

  • Predicting rates of cell state change caused by stochastic fluctuations using a data-driven landscape model.

    abstract：:We develop a potential landscape approach to quantitatively describe experimental data from a fibroblast cell line that exhibits a wide range of GFP expression levels under the control of the promoter for tenascin-C. Time-lapse live-cell microscopy provides data about short-term fluctuations in promoter activity, and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1207544109

    authors： Sisan DR,Halter M,Hubbard JB,Plant AL

    更新日期：2012-11-20 00:00:00

  • Interaction of Escherichia coli 30S ribosomal subunits with MS2 phage RNA in the absence of initiation factors.

    abstract：:MS2 RNA binds at 0 degrees to 30S subunits from E. coli and, to a smaller extent, to those of a Pseudomonas species, as judged by filtration on nitrocellulose membranes; this mRNA does not bind to 30S subunits from Bacillus brevis or Caulobacter crescentus. Binding does not depend on the presence of initiation factors...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.9.3611

    authors： Szer W,Leffler S

    更新日期：1974-09-01 00:00:00

  • Molecular structure of monomorphic peptide fibrils within a kinetically trapped hydrogel network.

    abstract：:Most, if not all, peptide- and protein-based hydrogels formed by self-assembly can be characterized as kinetically trapped 3D networks of fibrils. The propensity of disease-associated amyloid-forming peptides and proteins to assemble into polymorphic fibrils suggests that cross-β fibrils comprising hydrogels may also ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1509313112

    authors： Nagy-Smith K,Moore E,Schneider J,Tycko R

    更新日期：2015-08-11 00:00:00