Predicting rates of cell state change caused by stochastic fluctuations using a data-driven landscape model.

abstract：:Gonadotropin-releasing hormone (GnRH) is important in reproduction, although the mechanism of central hypogonadism in humans remains unclear. Because the GnRH neuron originates from the olfactory placode and migrates to the hypothalamus during development, central hypogonadism in humans could be caused by failure in n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Radovick S,Wray S,Lee E,Nicols DK,Nakayama Y,Weintraub BD,Westphal H,Cutler GB Jr,Wondisford FE

更新日期：1991-04-15 00:00:00

abstract：:There is great interest in developing selective protein kinase inhibitors by targeting allosteric sites, but these sites often involve protein-protein or protein-peptide interfaces that are very challenging to target with small molecules. Here we present a systematic approach to targeting a functionally conserved allo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rettenmaier TJ,Sadowsky JD,Thomsen ND,Chen SC,Doak AK,Arkin MR,Wells JA

更新日期：2014-12-30 00:00:00

abstract：:Bacillus anthrax lethal toxin can be engineered to deliver foreign proteins to the cytosol for antigen presentation to CD8 T cells. Vaccination with modified toxins carrying 8-9 amino acid peptide epitopes induces protective immunity in mice. To evaluate whether large protein antigens can be used with this system, rec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Y,Friedman R,Kushner N,Doling A,Thomas L,Touzjian N,Starnbach M,Lieberman J

更新日期：2000-07-05 00:00:00

abstract：:Vascular endothelial dysfunction underlies the genesis and progression of numerous diseases. Although the GATA transcription factor GATA-2 is expressed in endothelial cells and is implicated in coronary heart disease, it has been studied predominantly as a master regulator of hematopoiesis. Because many questions rega...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Linnemann AK,O'Geen H,Keles S,Farnham PJ,Bresnick EH

更新日期：2011-08-16 00:00:00

abstract：:Ciliary Neurotrophic Factor (CNTF) was first characterized as a trophic factor for motor neurons in the ciliary ganglion and spinal cord, leading to its evaluation in humans suffering from motor neuron disease. In these trials, CNTF caused unexpected and substantial weight loss, raising concerns that it might produce ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lambert PD,Anderson KD,Sleeman MW,Wong V,Tan J,Hijarunguru A,Corcoran TL,Murray JD,Thabet KE,Yancopoulos GD,Wiegand SJ

更新日期：2001-04-10 00:00:00

abstract：:Dust grains of organic matter were the main reservoir of C and N in the forming Solar System and are thus considered to be an essential ingredient for the emergence of life. However, the physical environment and the chemical mechanisms at the origin of these organic grains are still highly debated. In this study, we r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tartèse R,Chaussidon M,Gurenko A,Delarue F,Robert F

更新日期：2018-08-21 00:00:00

abstract：:When the lactose (lac) permease of Escherichia coli is expressed from the lac promoter at relatively low rates, deletion of amino acid residues 2-8 (delta 7) or 2-9 (delta 8) from the hydrophilic N terminus has a relatively minor effect on the ability of the permease to catalyze active lactose transport. Activity is e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bibi E,Stearns SM,Kaback HR

更新日期：1992-04-15 00:00:00

abstract：:The regulatory mechanisms that use signals of low levels of reactive oxygen species (ROS) could be obscured by ROS produced under stress and thus are better investigated under homeostatic conditions. Previous studies showed that the chloroplastic atypical thioredoxin ACHT1 is oxidized by 2-Cys peroxiredoxin (2-Cys Prx...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eliyahu E,Rog I,Inbal D,Danon A

更新日期：2015-10-13 00:00:00

abstract：:Alzheimer's disease (AD), characterized by cognitive decline, has emerged as a disease of synaptic failure. The present study reveals an unanticipated role of erythropoietin-producing hepatocellular A4 (EphA4) in mediating hippocampal synaptic dysfunctions in AD and demonstrates that blockade of the ligand-binding dom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu AK,Hung KW,Huang H,Gu S,Shen Y,Cheng EY,Ip FC,Huang X,Fu WY,Ip NY

更新日期：2014-07-08 00:00:00

abstract：:By using the maximum likelihood method, we made a genome-wide comparison of the evolutionary rates in the lineages leading to the laboratory strain (S288c) and a wild strain (YJM789) of Saccharomyces cerevisiae and found that genes in the laboratory strain tend to evolve faster than in the wild strain. The pattern of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gu Z,David L,Petrov D,Jones T,Davis RW,Steinmetz LM

更新日期：2005-01-25 00:00:00

abstract：:Seizure activity regulates gene expression for glial cell line-derived neurotrophic factor (GDNF) and neurturin (NRTN), and their receptor components, the transmembrane c-Ret tyrosine kinase and the glycosylphosphatidylinositol-anchored GDNF family receptor (GFR) alpha 1 and alpha 2 in limbic structures. We demonstrat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nanobashvili A,Airaksinen MS,Kokaia M,Rossi J,Asztély F,Olofsdotter K,Mohapel P,Saarma M,Lindvall O,Kokaia Z

更新日期：2000-10-24 00:00:00

abstract：:Analyses of amino-acid sequences of the total cell-free products programmed by the mRNA of MOPC-104E gamma light (L)-chain show that over 95% of the products have sequences of a distinct protein that correspond to the L-chain precursor. In this precursor an extra piece is coupled to the NH2-terminus of the mature L-ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burstein Y,Kantour F,Schechter I

更新日期：1976-08-01 00:00:00

abstract：:Overexpression of human cyclooxygenase 2 (COX-2) in the mammary glands of transgenic mice induces tissue-specific tumorigenic transformation. However, the molecular mechanisms involved are not yet defined. Here we show that COX-2 expressed in the epithelial cell compartment regulates angiogenesis in the stromal tissue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang SH,Liu CH,Conway R,Han DK,Nithipatikom K,Trifan OC,Lane TF,Hla T

更新日期：2004-01-13 00:00:00

abstract：:We have investigated whether glycosylation of membrane glycoproteins is a determinant of the site of maturation of enveloped viruses in Madin-Darby canine kidney (MDCK) cells. In MDCK cell monolayers, vesicular stomatitis virus buds exclusively from the basal or lateral plasma membranes and contains a sialylated glyco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roth MG,Fitzpatrick JP,Compans RW

更新日期：1979-12-01 00:00:00

abstract：:Utilization of transcription factors might be a powerful approach to modification of metabolism for a generation of crops having superior characteristics because a single transcription factor frequently regulates coordinated expression of a set of key genes for respective pathways. Here, we apply the plant-specific Do...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yanagisawa S,Akiyama A,Kisaka H,Uchimiya H,Miwa T

更新日期：2004-05-18 00:00:00

abstract：:The method of matrix isolation has been used to investigate mechanisms of gas-phase chemical reactions, in particular the pyrolysis of some organomercury compounds. A molecular beam of pyrolysis products was condensed simultaneously with a large excess of rare gas at temperatures from 5 to 15 degrees K to form a matri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maltsev AK,Mikaelian RG,Nefedov OM,Hauge RH,Margrave JL

更新日期：1971-12-01 00:00:00

abstract：:Polyploidy, the presence of more than two complete sets of chromosomes in an organism, has significantly shaped the genomes of angiosperms during evolution. Two forms of polyploidy are often considered: allopolyploidy, which originates from interspecies hybrids, and autopolyploidy, which originates from intraspecies g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Z,Haberer G,Matthes M,Rattei T,Mayer KF,Gierl A,Torres-Ruiz RA

更新日期：2010-10-12 00:00:00

abstract：:The sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) is a P-type ATPase that transports Ca2+ from the cytosol into the sarco(endo)plasmic reticulum (SR/ER) lumen, driven by ATP. This primary transport activity depends on tight coupling between movements of the transmembrane helices forming the two Ca2+-binding sites a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geurts MMG,Clausen JD,Arnou B,Montigny C,Lenoir G,Corey RA,Jaxel C,Møller JV,Nissen P,Andersen JP,le Maire M,Bublitz M

更新日期：2020-12-08 00:00:00

abstract：:The long QT syndrome (LQTS) is a heritable disorder that predisposes to sudden cardiac death. LQTS is caused by mutations in ion channel genes including HERG and KCNE1, but the precise mechanisms remain unclear. To clarify this situation we injected adenoviral vectors expressing wild-type or LQT mutants of HERG and KC...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoppe UC,Marbán E,Johns DC

更新日期：2001-04-24 00:00:00

abstract：:We report here the discovery of a small molecule inhibitor of pestivirus replication. The compound, designated VP32947, inhibits the replication of bovine viral diarrhea virus (BVDV) in cell culture at a 50% inhibitory concentration of approximately 20 nM. VP32947 inhibits both cytopathic and noncytopathic pestiviruse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baginski SG,Pevear DC,Seipel M,Sun SC,Benetatos CA,Chunduru SK,Rice CM,Collett MS

更新日期：2000-07-05 00:00:00

abstract：:We have developed a technology for analysis and sorting of live cells according to secreted molecules. An artificial affinity matrix, specific for the secreted product of interest, is created on the cell surface, and the cells are allowed to secrete for a defined time period. The secreted molecules bind to the affinit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manz R,Assenmacher M,Pflüger E,Miltenyi S,Radbruch A

更新日期：1995-03-14 00:00:00

abstract：:Transmission electron microscopy studies have been used to argue that magnetite crystals in carbonate from Martian meteorite ALH84001 have a composition and morphology indistinguishable from that of magnetotactic bacteria. It has even been claimed from scanning electron microscopy imaging that some ALH84001 magnetite ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss BP,Kim SS,Kirschvink JL,Kopp RE,Sankaran M,Kobayashi A,Komeili A

更新日期：2004-06-01 00:00:00

abstract：:The field of perceptual learning has identified changes in perceptual templates as a powerful mechanism mediating the learning of statistical regularities in our environment. By measuring threshold-vs.-contrast curves using an orientation identification task under varying levels of external noise, the perceptual templ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bejjanki VR,Zhang R,Li R,Pouget A,Green CS,Lu ZL,Bavelier D

更新日期：2014-11-25 00:00:00

abstract：:While the general structure of immunoglobulin chains has remained relatively unchanged throughout evolution, the organization of the genes encoding these molecules differs substantially. To understand how the rearranging immunoglobulin system arose, it is necessary to examine living representatives of the most early v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hohman VS,Schuchman DB,Schluter SF,Marchalonis JJ

更新日期：1993-11-01 00:00:00

abstract：:Assembly of protein complexes is a key mechanism for achieving spatial and temporal coordination in processes involving many enzymes. Growth of rod-shaped bacteria is a well-studied example requiring such coordination; expansion of the cell wall is thought to involve coordination of the activity of synthetic enzymes w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee TK,Tropini C,Hsin J,Desmarais SM,Ursell TS,Gong E,Gitai Z,Monds RD,Huang KC

更新日期：2014-03-25 00:00:00

abstract：:The density of benzodiazepine/gamma-aminobutyric acid receptor binding sites was lower in the midbrain of seizure-susceptible gerbils compared to control seizure-resistant gerbils. Binding of [3H]diazepam to high-affinity brain-specific sites in membrane homogenates of gerbil brain showed a 20-30% lower binding in mid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olsen RW,Wamsley JK,McCabe RT,Lee RJ,Lomax P

更新日期：1985-10-01 00:00:00

abstract：:Defects in clearing apoptotic debris disrupt tissue and immunological homeostasis, leading to autoimmune and inflammatory diseases. Herein, we report that macrophages from lupus-prone MRL/lpr mice have impaired lysosomal maturation, resulting in heightened ROS production and attenuated lysosomal acidification. Impaire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Monteith AJ,Kang S,Scott E,Hillman K,Rajfur Z,Jacobson K,Costello MJ,Vilen BJ

更新日期：2016-04-12 00:00:00

abstract：:It is a well known fact that stirring keeps particles suspended in fluids. This is apparent, for instance, when shaking medicine flasks, when agitating tea deposits in a mug, or when heavy winds fill the air with dust particles. The commonplace nature of such observations makes it easy to accept that this feature will...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruiz J,Macías D,Peters F

更新日期：2004-12-21 00:00:00

abstract：:We have shown that cellular inhibitor of apoptosis proteins (cIAPs) impair clearance of hepatitis B virus (HBV) infection by preventing TNF-mediated killing/death of infected cells. A key question, with profound therapeutic implications, is whether this finding can be translated to the development of drugs that promot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ebert G,Allison C,Preston S,Cooney J,Toe JG,Stutz MD,Ojaimi S,Baschuk N,Nachbur U,Torresi J,Silke J,Begley CG,Pellegrini M

更新日期：2015-05-05 00:00:00

abstract：:Our recent studies have shown that deregulated expression of R2, the rate-limiting component of ribonucleotide reductase, enhances transformation and malignant potential by cooperating with activated oncogenes. We now demonstrate that the R1 component of ribonucleotide reductase has tumor-suppressing activity. Stable ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan H,Huang A,Villegas C,Wright JA

更新日期：1997-11-25 00:00:00