Polarity of influenza and vesicular stomatitis virus maturation in MDCK cells: lack of a requirement for glycosylation of viral glycoproteins.

abstract：:Cancer stem cells (CSCs) sustain tumor growth through their ability to self-renew and to generate differentiated progeny. These functions endow CSCs with the potential to initiate secondary tumors bearing characteristics similar to those of the parent. Recently the hair follicle stem cell marker CD34 was used to purif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schober M,Fuchs E

更新日期：2011-06-28 00:00:00

abstract：:Genomic clones and cDNA plasmids were isolated for the intermediate filament protein vimentin from chicken. The identity of the various clones was determined both by mRNA selection [Paterson, B. M. & Roberts, B. E. (1981) in Gene Amplification and Analysis, Structural Analysis or Nucleic Acids, eds. Chirikjian, J. G. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zehner ZE,Paterson BM

更新日期：1983-02-01 00:00:00

abstract：:The tumor suppressor protein p53 plays a crucial role in coordinating cellular processes, such as cell cycle arrest, apoptosis, and senescence. The nerve injury-induced protein 1 (Ninjurin1, Ninj1) is a homophilic adhesion molecule and involved in nerve regeneration. Interestingly, Ninj1 is found to be overexpressed i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho SJ,Rossi A,Jung YS,Yan W,Liu G,Zhang J,Zhang M,Chen X

更新日期：2013-06-04 00:00:00

abstract：:Cytochrome c oxidase, the terminal protein in the respiratory chain, converts oxygen into water and helps generate the electrochemical gradient used in the synthesis of ATP. The catalytic action of cytochrome c oxidase involves electron transfer, proton transfer, and O2 reduction. These events trigger specific molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nyquist RM,Heitbrink D,Bolwien C,Gennis RB,Heberle J

更新日期：2003-07-22 00:00:00

abstract：:Thermus thermophilus possesses an aspartyl-tRNA synthetase (AspRS2) able to aspartylate efficiently tRNAAsp and tRNAAsn. Aspartate mischarged on tRNAAsn then is converted into asparagine by an omega amidase that differs structurally from all known asparagine synthetases. However, aspartate is not misincorporated into ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker HD,Kern D

更新日期：1998-10-27 00:00:00

abstract：:Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 X D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the sam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miskimins R,Ebato H,Seyfried TN,Yu RK

更新日期：1986-03-01 00:00:00

abstract：:Swarming and mass migration are spectacular and sometimes devastating features of the biology of various animal species. These phenomena are typically associated with actual or anticipated depletion of food resources after an increase in population density, but the mechanisms driving such collective movements are poor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simpson SJ,Sword GA,Lorch PD,Couzin ID

更新日期：2006-03-14 00:00:00

abstract：:Substantial proportions of mammalian genomes comprise repetitive elements including endogenous retrotransposons. Although these play diverse roles during development, their appropriate silencing is critically important in maintaining genomic integrity in the host cells. The major mechanism for retrotransposon silencin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hatanaka Y,Inoue K,Oikawa M,Kamimura S,Ogonuki N,Kodama EN,Ohkawa Y,Tsukada Y,Ogura A

更新日期：2015-11-24 00:00:00

abstract：:Niemann-Pick type C (NPC) 1 protein plays important roles in moving cholesterol and other lipids out of late endosomes by means of vesicular trafficking, but it is not known whether NPC1 directly interacts with cholesterol. We performed photoaffinity labeling of intact cells expressing fluorescent protein (FP)-tagged ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohgami N,Ko DC,Thomas M,Scott MP,Chang CC,Chang TY

更新日期：2004-08-24 00:00:00

abstract：:To examine the in vivo role(s) of type I interferons (IFNs) and to determine the role of a component of the type I IFN receptor (IFNAR1) in mediating responses to these IFNs, we generated mice with a null mutation (-/-) in the IFNAR1 gene. Despite compelling evidence for modulation of cell proliferation and differenti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang SY,Hertzog PJ,Holland KA,Sumarsono SH,Tymms MJ,Hamilton JA,Whitty G,Bertoncello I,Kola I

更新日期：1995-11-21 00:00:00

abstract：:Lithium ion at concentrations between 2 and 20 mM simulated the stimulatory effects of insulin on the uptake of alpha-aminoisobutyric acid, synthesis of RNA and DNA, and cell multiplication in mouse mammary gland explants cultured in a chemically defined synthetic medium. Other monovalent cations were virtually ineffe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hori C,Oka T

更新日期：1979-06-01 00:00:00

abstract：:Cardiomyopathy (CM) is a primary degenerative disease of myocardium and is traditionally categorized into hypertrophic and dilated CMs (HCM and DCM) according to its gross appearance. Cardiomyopathic hamster (CM hamster), a representative model of human hereditary CM, has HCM and DCM inbred sublines, both of which des...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakamoto A,Ono K,Abe M,Jasmin G,Eki T,Murakami Y,Masaki T,Toyo-oka T,Hanaoka F

更新日期：1997-12-09 00:00:00

abstract：:Investigations using the fruit fly Drosophila melanogaster have shown that the circadian clock gene period (Per) can influence behavioral responses to cocaine. Here we show that the mouse homologues of the Drosophila Per gene, mPer1 and mPer2, modulate cocaine sensitization and reward, two phenomena extensively studie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abarca C,Albrecht U,Spanagel R

更新日期：2002-06-25 00:00:00

abstract：:Salt and water secretion from intestinal epithelia requires enhancement of anion permeability across the apical membrane of Cl- secreting cells lining the crypt, the secretory gland of the intestine. Paneth cells located at the base of the small intestinal crypt release enteric defensins (cryptdins) apically into the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lencer WI,Cheung G,Strohmeier GR,Currie MG,Ouellette AJ,Selsted ME,Madara JL

更新日期：1997-08-05 00:00:00

abstract：:In a study of juvenile hormone-regulated gene expression, we isolated an anonymous cDNA representing a message that was strongly suppressed by juvenile hormone in the fat body of the cockroach Blaberus discoidalis. The protein deduced from the cDNA sequence showed compelling resemblance in sequence to the transferrins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamroz RC,Gasdaska JR,Bradfield JY,Law JH

更新日期：1993-02-15 00:00:00

abstract：:c-Jun transcriptional activity is augmented by expression of oncogenic Ras and Raf proteins. This study demonstrates a direct correlation between Ras transforming activity and c-Jun activation, supporting an important role for c-Jun in transformation by Ras. Since we observed that Ras activated c-Jun transcriptional a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Westwick JK,Cox AD,Der CJ,Cobb MH,Hibi M,Karin M,Brenner DA

更新日期：1994-06-21 00:00:00

abstract：:In our earlier work we showed that either spermidine or spermine could support the growth of spe2Delta or spe3Delta polyamine-requiring mutants, but it was unclear whether the cells had a specific requirement for either of these amines. In the current work, we demonstrate that spermidine is specifically required for t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chattopadhyay MK,Tabor CW,Tabor H

更新日期：2003-11-25 00:00:00

abstract：:Several Bradyrhizobium species nodulate the leguminous plant Aeschynomene indica in a type III secretion system-dependent manner, independently of Nod factors. To date, the underlying molecular determinants involved in this symbiotic process remain unknown. To identify the rhizobial effectors involved in nodulation, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teulet A,Busset N,Fardoux J,Gully D,Chaintreuil C,Cartieaux F,Jauneau A,Comorge V,Okazaki S,Kaneko T,Gressent F,Nouwen N,Arrighi JF,Koebnik R,Mergaert P,Deslandes L,Giraud E

更新日期：2019-10-22 00:00:00

abstract：:[PSI+] is a genetic element in yeast for which a heritable change in phenotype appears to be caused by a heritable change in the conformational state of the Sup35 protein. The inheritance of [PSI+] and the physical state of Sup35 in vivo depend on the protein chaperone Hsp104 (heat shock protein 104). Although these o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schirmer EC,Lindquist S

更新日期：1997-12-09 00:00:00

abstract：:Regeneration of appendages is frequent among invertebrates as well as some vertebrates. However, in mammals this has been largely relegated to digit tip regeneration, as found in mice and humans. The regenerated structures are formed from a mound of undifferentiated cells called a blastema, found just below the site o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehoczky JA,Robert B,Tabin CJ

更新日期：2011-12-20 00:00:00

abstract：:Cytoplasmic dynein is a microtubule-based motor protein complex that plays important roles in a wide range of fundamental cellular processes, including vesicular transport, mitosis, and cell migration. A single major form of cytoplasmic dynein associates with membranous organelles, mitotic kinetochores, the mitotic an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams JC,Roulhac PL,Roy AG,Vallee RB,Fitzgerald MC,Hendrickson WA

更新日期：2007-06-12 00:00:00

abstract：:A combined population genetic and reproductive analysis was undertaken to compare free-ranging cheetahs from east Africa (Acinonyx jubatus raineyi) with the genetically impoverished and reproductively impaired south African subspecies (Acinonyx jubatus jubatus). Like that of their south African counterparts, the quali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Brien SJ,Wildt DE,Bush M,Caro TM,FitzGibbon C,Aggundey I,Leakey RE

更新日期：1987-01-01 00:00:00

abstract：:Genes that are unexpressed in somatic cells have been activated by injecting cultured cell nuclei of the frog Xenopus laevis into oocytes of the newt Pleurodeles waltlii. The genes that were activated are normally expressed in oocytes but not in cultured cells. Conversely, genes that are normally expressed in cultured...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Robertis EM,Gurdon JB

更新日期：1977-06-01 00:00:00

abstract：:Recombinant full-length CD4 expressed in Spodoptera frugiperda 9 cells with the baculovirus system was electroinserted in erythrocyte (RBC) membranes. Of the inserted CD4, 70% was "correctly" oriented as shown by fluorescence quenching experiments with fluorescein-labeled CD4. The inserted CD4 displayed the same epito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeira M,Tosi PF,Mouneimne Y,Lazarte J,Sneed L,Volsky DJ,Nicolau C

更新日期：1991-05-15 00:00:00

abstract：:The 2-deoxy-D-glucose method was used to study asymmetries in cerebral metabolic activity in neonatal rats. Left-right asymmetries in 2-deoxy-D-glucose uptake were observed in hippocampus, diencephalon, cortex, and medulla-pons: 2-deoxy-D-glucose incorporation was greater in right hippocampus, right diencephalon, left...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ross DA,Glick SD,Meibach RC

更新日期：1981-03-01 00:00:00

abstract：:Mechanotransduction in the mammalian auditory system depends on mechanosensitive channels in the hair bundles that project from the apical surface of the sensory hair cells. Individual stereocilia within each bundle contain a core of tightly packed actin filaments, whose length is dynamically regulated during developm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furness DN,Johnson SL,Manor U,Rüttiger L,Tocchetti A,Offenhauser N,Olt J,Goodyear RJ,Vijayakumar S,Dai Y,Hackney CM,Franz C,Di Fiore PP,Masetto S,Jones SM,Knipper M,Holley MC,Richardson GP,Kachar B,Marcotti W

更新日期：2013-08-20 00:00:00

abstract：:The phosphatase and tensin homolog (PTEN) is a tumor suppressor that is inactivated in many human cancers. PTEN loss has been associated with resistance to inhibitors of the epidermal growth factor receptor (EGFR), but the molecular basis of this resistance is unclear. It is believed that unopposed phosphatidylinosito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vivanco I,Rohle D,Versele M,Iwanami A,Kuga D,Oldrini B,Tanaka K,Dang J,Kubek S,Palaskas N,Hsueh T,Evans M,Mulholland D,Wolle D,Rajasekaran S,Rajasekaran A,Liau LM,Cloughesy TF,Dikic I,Brennan C,Wu H,Mischel PS,

更新日期：2010-04-06 00:00:00

abstract：:Endothelial cells (EC) play a central role in inflammatory immune responses and efficiently induce effector functions in T cells, despite lacking the classical costimulatory ligands CD80 and CD86. By using the mAb HIL-131 we now demonstrate that human inducible costimulator-ligand (ICOS-L), a molecule related to CD80/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khayyamian S,Hutloff A,Büchner K,Gräfe M,Henn V,Kroczek RA,Mages HW

更新日期：2002-04-30 00:00:00

abstract：:Certain complex phenotypes appear repeatedly across diverse species due to processes of evolutionary conservation and convergence. In some contexts like developmental body patterning, there is increased appreciation that common molecular mechanisms underlie common phenotypes; these molecular mechanisms include highly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rittschof CC,Bukhari SA,Sloofman LG,Troy JM,Caetano-Anollés D,Cash-Ahmed A,Kent M,Lu X,Sanogo YO,Weisner PA,Zhang H,Bell AM,Ma J,Sinha S,Robinson GE,Stubbs L

更新日期：2014-12-16 00:00:00

abstract：:To study the role of the interferon- (IFN) gammaR2 chain in IFN-gamma signaling and immune function, IFN-gammaR2-deficient mice have been generated and characterized. Cells derived from IFN-gammaR2 -/- mice are unable to activate either JAK/STAT signaling proteins or gene transcription in response to IFN-gamma. The la...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu B,Ebensperger C,Dembic Z,Wang Y,Kvatyuk M,Lu T,Coffman RL,Pestka S,Rothman PB

更新日期：1998-07-07 00:00:00