Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex.

abstract：:Leptin acts as a potent inhibitory factor against obesity by regulating energy expenditure, food intake, and adiposity. The obese diabetic db/db mouse, which has defects in leptin receptor, displays enhanced neural responses and elevated behavioral preference to sweet stimuli. Here, we show the effects of leptin on th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawai K,Sugimoto K,Nakashima K,Miura H,Ninomiya Y

更新日期：2000-09-26 00:00:00

abstract：:A mutation partially suppressing the UV sensitivity caused by recF143 in a uvrA6 background was located at codon 37 of recA where GTG (valine) became ATG (methionine). This mutation, originally named srf-803, was renamed recA803. Little if any suppression of the recF143 defect in UV induction of a lexA regulon promote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Madiraju MV,Templin A,Clark AJ

更新日期：1988-09-01 00:00:00

abstract：:Interaction between a peptide hormone and extracellular domains of its receptor is a crucial step for initiation of hormone action. We have developed a modification of the yeast two-hybrid system to study this interaction and have used it to characterize the interaction of insulin-like growth factor 1 (IGF-1) with its...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu J,Kahn CR

更新日期：1997-11-25 00:00:00

abstract：:Tuberculosis (TB) is a major public health problem. One-third of the world's population is estimated to be infected with Mycobacterium tuberculosis (MTB), the etiological agent causing TB, and active disease kills nearly 2 million individuals worldwide every year. Several lines of evidence indicate that interindividua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barreiro LB,Tailleux L,Pai AA,Gicquel B,Marioni JC,Gilad Y

更新日期：2012-01-24 00:00:00

abstract：:A critique is presented of the Lumsden-Wilson theory [Lumsden, C. J. & Wilson, E. O. (1980) Proc. Natl. Acad. Sci. USA 77, 4382-4386] of the transmission of cultural traits. An analysis of the underlying assumptions and the mathematical nature of the theory clarifies its essentially reductionist and determinist qualit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alper JS,Lange RV

更新日期：1981-06-01 00:00:00

abstract：:We have used in vitro motility assays to investigate the mechanism of kinetochore function in the budding yeast Saccharomyces cerevisiae. Functional centromeric DNA plus a tripartite centromere binding protein complex, CBF3, was found to be necessary but not sufficient for in vitro kinetochore activity. A fourth requi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Middleton K,Carbon J

更新日期：1994-07-19 00:00:00

abstract：:Ultrastructural cytochemistry of natural killer cells enriched by Percoll gradient centrifugation showed them to possess arylsulfatase (aryl-sulfate sulfohydrolase, EC 3.1.6.1). The enzyme was located in vesicles, granules, and the parallel tubular arrays, organelles characteristic for cytotoxic lymphocytes. Biochemic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zucker-Franklin D,Grusky G,Yang JS

更新日期：1983-11-01 00:00:00

abstract：:We have long known that language is lateralized to the left hemisphere (LH) in most neurologically healthy adults. In contrast, findings on lateralization of function during development are more complex. As in adults, anatomical, electrophysiological, and neuroimaging studies in infants and children indicate LH latera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olulade OA,Seydell-Greenwald A,Chambers CE,Turkeltaub PE,Dromerick AW,Berl MM,Gaillard WD,Newport EL

更新日期：2020-09-22 00:00:00

abstract：:Protein-based hydrogels have emerged as promising alternatives to synthetic hydrogels for biomedical applications, owing to the precise control of structure and function enabled by protein engineering. Nevertheless, strategies for assembling 3D molecular networks that carry the biological information encoded in full-l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun F,Zhang WB,Mahdavi A,Arnold FH,Tirrell DA

更新日期：2014-08-05 00:00:00

abstract：:Brefeldin A, a fungal metabolite that inhibits membrane transport, induces the mono(ADP-ribosyl)ation of two cytosolic proteins of 38 and 50 kDa as judged by SDS/PAGE. The 38-kDa substrate has been previously identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report that the 50-kDa BFA-induced ADP-ribo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Girolamo M,Silletta MG,De Matteis MA,Braca A,Colanzi A,Pawlak D,Rasenick MM,Luini A,Corda D

更新日期：1995-07-18 00:00:00

abstract：:Many studies have reported age-related changes in immune responses that could be due to alterations in lymphoid cell numbers or functions. Here we report the results of studies using immunofluorescent staining and in vitro assays of cellular function to compare the expression of cell surface antigens on lymphocytes fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sidman CL,Luther EA,Marshall JD,Nguyen KA,Roopenian DC,Worthen SM

更新日期：1987-11-01 00:00:00

abstract：:Transcription is a central step in gene expression, in which the DNA template is processively read by RNA polymerase II (Pol II), synthesizing a complementary messenger RNA transcript. At each cycle, Pol II moves exactly one register along the DNA, a process known as translocation. Although X-ray crystal structures ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silva DA,Weiss DR,Pardo Avila F,Da LT,Levitt M,Wang D,Huang X

更新日期：2014-05-27 00:00:00

abstract：:Protein tyrosine phosphatase (PTP)σ (PTPRS) was shown previously to be associated with susceptibility to inflammatory bowel disease (IBD). PTPσ(-/-) mice exhibit an IBD-like phenotype in the intestine and show increased susceptibility to acute models of murine colitis. However, the function of PTPσ in the intestine is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murchie R,Guo CH,Persaud A,Muise A,Rotin D

更新日期：2014-01-14 00:00:00

abstract：:The Eastern Arc Mountains of Tanzania and the Atlantic Forest of Brazil are two of the most fragmented biodiversity hotspots. Species-area relationships predict that their habitat fragments will experience a substantial loss of species. Most of these extinctions will occur over an extended time, and therefore, reconne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newmark WD,Jenkins CN,Pimm SL,McNeally PB,Halley JM

更新日期：2017-09-05 00:00:00

abstract：:Converging TGF-beta and insulin-like neuroendocrine signaling pathways regulate whether Caenorhabditis elegans develops reproductively or arrests at the dauer larval stage. We examined whether neurotransmitters act in the dauer entry or recovery pathways. Muscarinic agonists promote recovery from dauer arrest induced ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tissenbaum HA,Hawdon J,Perregaux M,Hotez P,Guarente L,Ruvkun G

更新日期：2000-01-04 00:00:00

abstract：:Chromosomal RNA, which is a component of the chromosomes of higher organisms, and whose participation is required for sequence-specific interaction of chromosomal proteins with chromosomal DNA, occurs in chromosomes bound to DNA. We have found that the DNA sequences to which chromosomal RNA binds are repetitive ones. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sivolap YM,Bonner J

更新日期：1971-02-01 00:00:00

abstract：:Half-cystine peptic peptides representing the intrachain disulfide bonds of the light chain from an apparently homogeneous rabbit anti-p-azobenzoate antibody were isolated in good yields after reduction and alkylation with [(14)C]iodoacetate, and their sequences were determined. The sequence of the first 21 amino-term...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Appella E,Chersi A,Roholt OA,Pressman D

更新日期：1971-10-01 00:00:00

abstract：:Plasmonics now delivers sensors capable of detecting single molecules. The emission enhancements and nanometer-scale optical confinement achieved by these metallic nanostructures vastly increase spectroscopic sensitivity, enabling real-time tracking. However, the interaction of light with such nanostructures typically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horton MJ,Ojambati OS,Chikkaraddy R,Deacon WM,Kongsuwan N,Demetriadou A,Hess O,Baumberg JJ

更新日期：2020-02-04 00:00:00

abstract：:Sheep antibodies directed against an AMP-bovine serum albumin conjugate exhibit highly specific binding toward AMP. These antibodies bind to the AMP moiety of adenylylated glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] from Escherichia coli and to no other antigenic determinant on the prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hohman RJ,Rhee SG,Stadtman ER

更新日期：1980-12-01 00:00:00

abstract：:Dietary restriction has been shown to have several health benefits including increased insulin sensitivity, stress resistance, reduced morbidity, and increased life span. The mechanism remains unknown, but the need for a long-term reduction in caloric intake to achieve these benefits has been assumed. We report that w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anson RM,Guo Z,de Cabo R,Iyun T,Rios M,Hagepanos A,Ingram DK,Lane MA,Mattson MP

更新日期：2003-05-13 00:00:00

abstract：:Genetic studies in mice suggest that Wnt4 signaling antagonizes expression of male hormones and effectively blocks male development in the female embryo. We recently identified an XY intersex patient carrying a chromosomal duplication of the WNT4 locus and proposed that this patient's feminization arises from an incre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jordan BK,Shen JH,Olaso R,Ingraham HA,Vilain E

更新日期：2003-09-16 00:00:00

abstract：:High-resolution single nucleotide polymorphism genomic microarray (SNP-chip) is a useful tool to define gene dosage levels over the whole genome, allowing precise detection of deletions and duplications/amplifications of chromosomes in cancer cells. We found that this new technology can also identify breakpoints of ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawamata N,Ogawa S,Zimmermann M,Niebuhr B,Stocking C,Sanada M,Hemminki K,Yamatomo G,Nannya Y,Koehler R,Flohr T,Miller CW,Harbott J,Ludwig WD,Stanulla M,Schrappe M,Bartram CR,Koeffler HP

更新日期：2008-08-19 00:00:00

abstract：:Musical training is known to modify cortical organization. Here, we show that such modifications extend to subcortical sensory structures and generalize to processing of speech. Musicians had earlier and larger brainstem responses than nonmusician controls to both speech and music stimuli presented in auditory and aud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musacchia G,Sams M,Skoe E,Kraus N

更新日期：2007-10-02 00:00:00

abstract：:Parkinson's disease (PD) is a neurodegenerative disorder of uncertain pathogenesis characterized by a loss of substantia nigra pars compacta (SNpc) dopaminergic (DA) neurons, and can be modeled by the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Both inflammatory processes and oxidative stress may c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu DC,Teismann P,Tieu K,Vila M,Jackson-Lewis V,Ischiropoulos H,Przedborski S

更新日期：2003-05-13 00:00:00

abstract：:Membrane lipids were once thought to be homogenously distributed in the 2D surface of a membrane, but the lipid raft theory suggests that cholesterol and sphingolipids partition away from other membrane lipids. Lipid raft theory further implicates these cholesterol-rich domains in many processes such as signaling and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foster LJ,De Hoog CL,Mann M

更新日期：2003-05-13 00:00:00

abstract：:Lizards and snakes putatively arose between the early Jurassic and late Triassic; they diversified worldwide and now occupy many different ecological niches, making them ideal for testing theories on the origin of ecological traits. We propose and test the "deep history hypothesis," which claims that differences in ec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vitt LJ,Pianka ER

更新日期：2005-05-31 00:00:00

abstract：:Activation of eukaryotic class II gene expression involves the formation of a transcription initiation complex that includes RNA polymerase II, general transcription factors, and SRB components of the holoenzyme. Negative regulators of transcription have been described, but it is not clear whether any are general repr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gadbois EL,Chao DM,Reese JC,Green MR,Young RA

更新日期：1997-04-01 00:00:00

abstract：:A polyclonal antiserum directed against the C-terminal domain of dystrophin was used to isolate a cDNA clone encoding an antigenically cross-reactive protein, microtubule-associated protein 1B (MAP-1B). Physical mapping of the human MAP-1B locus places its chromosomal location at 5q13, in proximity to the spinal muscu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lien LL,Boyce FM,Kleyn P,Brzustowicz LM,Menninger J,Ward DC,Gilliam TC,Kunkel LM

更新日期：1991-09-01 00:00:00

abstract：:Antibody against carcinoembryonic antigen (CEA) was conjugated with p-[1,2-dicarba-closo-[1-3H]do-decaboran(12)-2-yl] benzenediazonium ion by an azo-coupling reaction, resulting in 30 boron atoms per IgG molecule with no loss of antibody protein. Antibody immunoreactivity was not appreciably affected by this conjugati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldenberg DM,Sharkey RM,Primus FJ,Mizusawa E,Hawthorne MF

更新日期：1984-01-01 00:00:00

abstract：:The folding, stability, and oligomerization of helical membrane proteins depend in part on a precise set of packing interactions between transmembrane helices. To understand the energetic principles of these helix-helix interactions, we have used alanine-scanning mutagenesis and sedimentation equilibrium analytical ul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleming KG,Engelman DM

更新日期：2001-12-04 00:00:00