Both hypertrophic and dilated cardiomyopathies are caused by mutation of the same gene, delta-sarcoglycan, in hamster: an animal model of disrupted dystrophin-associated glycoprotein complex.

abstract：:Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total hea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loike JD,Silverstein SC,Sturtevant JM

更新日期：1981-10-01 00:00:00

abstract：:Near infrared diffuse optical spectroscopy and diffuse optical imaging are promising methods that eventually may enhance or replace existing technologies for breast cancer screening and diagnosis. These techniques are based on highly sensitive, quantitative measurements of optical and functional contrast between healt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shah N,Cerussi A,Eker C,Espinoza J,Butler J,Fishkin J,Hornung R,Tromberg B

更新日期：2001-04-10 00:00:00

abstract：:We have shown previously that hydroxyl radicals (HO*) can be produced by H2O2 and halogenated quinones, independent of transition metal ions; however, the underlying molecular mechanism is still unclear. In the present study, using the electron spin resonance secondary radical spin-trapping method, we found that tetra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu BZ,Kalyanaraman B,Jiang GB

更新日期：2007-11-06 00:00:00

abstract：:Inhibition of mitochondrial complex I is one of the leading hypotheses for dopaminergic neuron death associated with Parkinson's disease (PD). To test this hypothesis genetically, we used a mouse strain lacking functional Ndufs4, a gene encoding a subunit required for complete assembly and function of complex I. Delet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi WS,Kruse SE,Palmiter RD,Xia Z

更新日期：2008-09-30 00:00:00

abstract：:Depending on the cellular context, BCL2-like proteins promote mitochondrial fusion or fission. What determines which of these two opposing processes they promote has so far been unknown. Furthermore, the mechanisms through which BCL2-like proteins affect mitochondrial dynamics remain to be fully understood. The BCL2-l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Y,Rolland SG,Conradt B

更新日期：2011-10-11 00:00:00

abstract：:CCAAT displacement protein (CDP), a nuclear protein of 180-190 kDa, contains a triplicated motif, the cut domain, similar (80-90% conserved) to three repeats of 60-65 amino acids first identified in Drosophila cut, a homeo-domain protein involved in cell-fate decisions in development. Cut repeats bind DNA and exhibit ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aufiero B,Neufeld EJ,Orkin SH

更新日期：1994-08-02 00:00:00

abstract：:The discovery of RNAi has revolutionized loss-of-function genetic studies in mammalian systems. However, significant challenges still remain to fully exploit RNAi for mammalian genetics. For instance, genetic screens and in vivo studies could be broadly improved by methods that allow inducible and uniform gene express...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meerbrey KL,Hu G,Kessler JD,Roarty K,Li MZ,Fang JE,Herschkowitz JI,Burrows AE,Ciccia A,Sun T,Schmitt EM,Bernardi RJ,Fu X,Bland CS,Cooper TA,Schiff R,Rosen JM,Westbrook TF,Elledge SJ

更新日期：2011-03-01 00:00:00

abstract：:The nucleolinus is an RNA-rich compartment, closely apposed to or embedded within the nucleolus. Discovered over 150 y ago, fewer than two dozen articles have been published on the nucleolinus, probably because complex histochemical stains are required for its visualization in the great majority of cells. The nucleoli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alliegro MA,Henry JJ,Alliegro MC

更新日期：2010-08-03 00:00:00

abstract：:We present whole-genome assemblies of four divergent Arabidopsis thaliana strains that complement the 125-Mb reference genome sequence released a decade ago. Using a newly developed reference-guided approach, we assembled large contigs from 9 to 42 Gb of Illumina short-read data from the Landsberg erecta (Ler-1), C24,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schneeberger K,Ossowski S,Ott F,Klein JD,Wang X,Lanz C,Smith LM,Cao J,Fitz J,Warthmann N,Henz SR,Huson DH,Weigel D

更新日期：2011-06-21 00:00:00

abstract：:Mutations in genes that encode components of the phosphatidyl-inositol 3-kinase (PI3-kinase) signaling pathway are common in human cancer. The recent discovery of nonrandom somatic mutations in the PIK3CA gene of many human tumors suggests an oncogenic role for the mutated enzyme. We have determined the growth-regulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang S,Bader AG,Vogt PK

更新日期：2005-01-18 00:00:00

abstract：:Wildfire can influence climate directly and indirectly, but little is known about the relationships between wildfire and climate during the Quaternary, especially how wildfire patterns varied over glacial-interglacial cycles. Here, we present a high-resolution soot record from the Chinese Loess Plateau; this is a reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han Y,An Z,Marlon JR,Bradley RS,Zhan C,Arimoto R,Sun Y,Zhou W,Wu F,Wang Q,Burr GS,Cao J

更新日期：2020-03-10 00:00:00

abstract：:The empirical criteria for defining a clinical subtype of lung cancer are gradually transiting from histopathology to genetic variations in driver genes. Targeting these driver mutations, such as sensitizing epidermal growth factor receptor (EGFR) mutations, has dramatically improved the prognosis of advanced non-smal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Z,Shen Z,Li Z,Duan J,Fu S,Liu Z,Bai H,Zhang Z,Zhao J,Wang X,Wang J

更新日期：2015-08-11 00:00:00

abstract：:Closed and pH-tight membrane vesicles prepared from hypocotyls of 5-day-old dark-grown seedlings of Cucurbita pepo accumulate the plant growth hormone indole-3-acetic acid along an imposed proton gradient (pH low outside, high inside). The use of electron paramagnetic spin probes permitted quantitation both of apparen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lomax TL,Mehlhorn RJ,Briggs WR

更新日期：1985-10-01 00:00:00

abstract：:The development of cardiac hypertrophy is mediated, in part, by increase in NADPH oxidase activity and myocardial oxidative stress. The Rho GTPase, Rac, regulates NADPH oxidase activity through interaction with gp91(phox) and p67(phox) (in which "phox" is phagocyte oxidase). However, it is not known which Rac isoform ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Satoh M,Ogita H,Takeshita K,Mukai Y,Kwiatkowski DJ,Liao JK

更新日期：2006-05-09 00:00:00

abstract：:A high-resolution compositional map of glass-forming ability (GFA) in the Ni-Cr-Nb-P-B system is experimentally determined along various compositional planes. GFA is shown to be a piecewise continuous function formed by intersecting compositional subsurfaces, each associated with a nucleation pathway for a specific cr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Na JH,Demetriou MD,Floyd M,Hoff A,Garrett GR,Johnson WL

更新日期：2014-06-24 00:00:00

abstract：:Incubation of chicken embryo fibroblasts with mitogenic concentrations of insulin for 24 hr or with the tumor promoter phorbol 12-myristate 13-acetate for 6 hr stimulated lactate release and 3-O-methylglucose uptake. Insulin also increased the Vmax of 6-phosphofructo-1-kinase (ATP:D-fructose-6-phosphate 1-phosphotrans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bosca L,Rousseau GG,Hue L

更新日期：1985-10-01 00:00:00

abstract：:Recently, we established that satellite III (TGGAA)n tandem repeats, which occur at the centromeres of human chromosomes, pair with themselves to form an unusual "self-complementary" antiparallel duplex containing (GGA)2 motifs in which two unpaired guanines from opposite strands intercalate between sheared G.A base p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu L,Chou SH,Reid BR

更新日期：1996-10-29 00:00:00

abstract：:Modern functional imaging techniques of the brain measure local hemodynamic responses evoked by neuronal activity. Capillary pericytes recently were suggested to mediate neurovascular coupling in brain slices, but their role in vivo remains unexplored. We used two-photon microscopy to study in real time pericytes and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernández-Klett F,Offenhauser N,Dirnagl U,Priller J,Lindauer U

更新日期：2010-12-21 00:00:00

abstract：:The science around the use of masks by the public to impede COVID-19 transmission is advancing rapidly. In this narrative review, we develop an analytical framework to examine mask usage, synthesizing the relevant literature to inform multiple areas: population impact, transmission characteristics, source control, wea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Howard J,Huang A,Li Z,Tufekci Z,Zdimal V,van der Westhuizen HM,von Delft A,Price A,Fridman L,Tang LH,Tang V,Watson GL,Bax CE,Shaikh R,Questier F,Hernandez D,Chu LF,Ramirez CM,Rimoin AW

更新日期：2021-01-26 00:00:00

abstract：:The ever-increasing global need for potable water requires practical, sustainable approaches for purifying abundant alternative sources such as seawater, high-salinity processed water, or underground reservoirs. Evaporation-based solutions are of particular interest for treating high salinity water, since conventional...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dongare PD,Alabastri A,Neumann O,Nordlander P,Halas NJ

更新日期：2019-07-02 00:00:00

abstract：:Development of spatial patterns in multicellular organisms depends on gradients in the concentration of signaling molecules that control gene expression. In the Drosophila embryo, Bicoid (Bcd) morphogen controls cell fate along 70% of the anteroposterior axis but is translated from mRNA localized at the anterior pole....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gregor T,Bialek W,de Ruyter van Steveninck RR,Tank DW,Wieschaus EF

更新日期：2005-12-20 00:00:00

abstract：:RNA molecules that bind a transition state analog for a Diels-Alder reaction (Kd = 0.35 +/- 0.05 mM) were isolated from a starting pool of approximately 10(14) sequences by affinity chromatography. After the initial rise and plateau of the amount of RNA that eluted with soluble analog, a step gradient elution was used...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morris KN,Tarasow TM,Julin CM,Simons SL,Hilvert D,Gold L

更新日期：1994-12-20 00:00:00

abstract：:A promoterless trp-lac fusion fragment was inserted near one end of the bacterial transposon Tn5 in the correct orientation to fuse lacZ gene expression to promoters outside Tn5. The resulting transposon, Tn5 lac, retains the kanamycin-resistance gene of Tn5 and transposes in Escherichia coli at 6% the frequency of Tn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kroos L,Kaiser D

更新日期：1984-09-01 00:00:00

abstract：:The optical transitions of a chiral, three-substituted polythiophene with an amino acid function can be tuned by interactions with synthetic peptides. The addition of a positively charged peptide with a random-coil formation will force the polymer to adopt a nonplanar conformation, and the intensity of the emitted lig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nilsson KP,Rydberg J,Baltzer L,Inganäs O

更新日期：2003-09-02 00:00:00

abstract：:We address the stability of multicharged finite systems driven by Coulomb forces beyond the Rayleigh instability limit. Our exploration of the nuclear dynamics of heavily charged Morse clusters enabled us to vary the range of the pair potential and of the fissibility parameter, which results in distinct fragmentation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Last I,Levy Y,Jortner J

更新日期：2002-07-09 00:00:00

abstract：:Bacteriophage N4 minivirion RNA polymerase (mini-vRNAP), the RNA polymerase (RNAP) domain of vRNAP, is a member of the T7-like RNAP family. Mini-vRNAP recognizes promoters that comprise conserved sequences and a 3-base loop-5-base pair (bp) stem DNA hairpin structure on single-stranded templates. Here, we defined the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davydova EK,Santangelo TJ,Rothman-Denes LB

更新日期：2007-04-24 00:00:00

abstract：:We study the physical origins of phase contrast in dynamic atomic force microscopy (dAFM) in liquids where low-stiffness microcantilever probes are often used for nanoscale imaging of soft biological samples with gentle forces. Under these conditions, we show that the phase contrast derives primarily from a unique ene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melcher J,Carrasco C,Xu X,Carrascosa JL,Gómez-Herrero J,José de Pablo P,Raman A

更新日期：2009-08-18 00:00:00

abstract：:The crossed strand-exchange form (Holliday structure, half chiasma) has been predicted as an intermediate in the genetic recombination of eukaryotes. We report here the detection of this form in the yeast plasmid, 2-micron DNA, isolated during meiosis. Physical mapping has previously suggested that two forms of 2-micr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bell L,Byers B

更新日期：1979-07-01 00:00:00

abstract：:The mechanisms for plant growth restriction during stress conditions remains unclear. Here, we demonstrate that a phytochrome-interacting factor-like protein, OsPIL1/OsPIL13, acts as a key regulator of reduced internode elongation in rice under drought conditions. The level of OsPIL1 mRNA in rice seedlings grown under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Todaka D,Nakashima K,Maruyama K,Kidokoro S,Osakabe Y,Ito Y,Matsukura S,Fujita Y,Yoshiwara K,Ohme-Takagi M,Kojima M,Sakakibara H,Shinozaki K,Yamaguchi-Shinozaki K

更新日期：2012-09-25 00:00:00

abstract：:Certain Escherichia coli mutants defective in phosphatidylglycerol biosynthesis accumulate two novel glycolipids, designated X and Y. Lipid X is a diacylglucosamine 1-phosphate bearing beta-hydroxymyristoyl groups at positions 2 and 3, and lipid Y has the same structure as X, except for the additional presence of a pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishijima M,Amano F,Akamatsu Y,Akagawa K,Tokunaga T,Raetz CR

更新日期：1985-01-01 00:00:00