Abstract:
:Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 X D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the same efficiency with respect to stimulation of amino acid incorporation into protein. However, a significant heterotic effect was seen for the production of myelin basic protein (MBP) by the mRNA from the F1 mice. That is, the fraction of protein synthesized as MBP was greater for the F1 hybrid than for either parental strain. The distribution of the form of MBPs was not different among the three strains. We therefore believe that heterosis for brain MBP content in the F1 hybrid may be regulated at the transcriptional or post-transcriptional level.
journal_name
Proc Natl Acad Sci U S Aauthors
Miskimins R,Ebato H,Seyfried TN,Yu RKdoi
10.1073/pnas.83.5.1532subject
Has Abstractpub_date
1986-03-01 00:00:00pages
1532-5issue
5eissn
0027-8424issn
1091-6490journal_volume
83pub_type
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