Abstract:
:We have isolated and partially characterized an endonuclease involved in processing the 5' end of 16S rRNA of Escherichia coli. A mutant strain that is deficient in this enzyme accumulates a new precursor of 16S rRNA, named 16.3S rRNA. This rRNA has the 3' end of mature 16S rRNA but is about 60 nucleotides longer at the 5' end. In vitro, the enzyme preparation cleaves an RNA fragment of about 60 nucleotides from the 5' end of 16.3S rRNA in 30S ribosomal subunits, yielding the mature 5' end of 16S rRNA. In the mutant strain the 16.3S rRNA is associated with a full complement of 21 ribosomal proteins in 30S subunits. These particles, which comprise 50% of the total 30S subunits, are present on polyribosomes.
journal_name
Proc Natl Acad Sci U S Aauthors
Dahlberg AE,Dahlberg JE,Lund E,Tokimatsu H,Rabson AB,Calvert PC,Reynolds F,Zahalak Mdoi
10.1073/pnas.75.8.3598subject
Has Abstractpub_date
1978-08-01 00:00:00pages
3598-602issue
8eissn
0027-8424issn
1091-6490journal_volume
75pub_type
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