Comprehensive copy number and gene expression profiling of the 17q23 amplicon in human breast cancer.

abstract：:RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular RNAi experiment. The ideal experiment is to rescue the phenotype by expression of the target gene in a form refractory ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kittler R,Pelletier L,Ma C,Poser I,Fischer S,Hyman AA,Buchholz F

更新日期：2005-02-15 00:00:00

abstract：:The free radicals nitric oxide and superoxide anion react to form peroxynitrite (ONOO-), a highly toxic oxidant species. In vivo formation of ONOO- has been demonstrated in shock and inflammation. Herein we provide evidence that cytotoxicity in cells exposed to ONOO- is mediated by DNA strand breakage and the subseque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szabó C,Zingarelli B,O'Connor M,Salzman AL

更新日期：1996-03-05 00:00:00

abstract：:A detailed model of the G(2) DNA damage checkpoint (G2DDC) system is presented that includes complex regulatory networks of the mitotic kinase Cdc2, phosphatase Cdc25, Wee1 kinase, and damage signal transduction pathways involving Chk1 and p53. Assumptions on the kinetic equations of the G2DDC are made, and computer s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aguda BD

更新日期：1999-09-28 00:00:00

abstract：:Due to intrinsic aggressiveness and lack of effective therapies, prognosis of pancreatic cancer remains dismal. Because the only molecular targeted drug approved for pancreatic ductal adenocarcinoma is a kinase inhibitor specific to the epidermal growth factor receptor (EGFR), and this receptor collaborates with anoth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maron R,Schechter B,Mancini M,Mahlknecht G,Yarden Y,Sela M

更新日期：2013-09-17 00:00:00

abstract：:In higher eukaryotic cells, mitochondria are essential subcellular organelles for energy production, cell signaling, and the biosynthesis of biomolecules. The mitochondrial DNA (mtDNA) genome is indispensable for mitochondrial function because it encodes protein subunits of the electron transport chain and a full set ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu W,Boyd RM,Tree MO,Samkari F,Zhao L

更新日期：2019-09-03 00:00:00

abstract：:Emotional signals are crucial for sharing important information, with conspecifics, for example, to warn humans of danger. Humans use a range of different cues to communicate to others how they feel, including facial, vocal, and gestural signals. We examined the recognition of nonverbal emotional vocalizations, such a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sauter DA,Eisner F,Ekman P,Scott SK

更新日期：2010-02-09 00:00:00

abstract：:In a coupled system consisting of RNA polymerase and DNA polymerase I of Escherichia coli, the four deoxyribo- and the four ribonucleoside triphosphates, and DNA of bacteriophage f1 as template, DNA synthesis depends on the concomitant synthesis of RNA. Over a wide range of concentrations of the two polymerases, RNA s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karkas JD

更新日期：1972-08-01 00:00:00

abstract：:For reasons that are unclear the production of embryonic stem cells from ungulates has proved elusive. Here, we describe induced pluripotent stem cells (iPSC) derived from porcine fetal fibroblasts by lentiviral transduction of 4 human (h) genes, hOCT4, hSOX2, hKLF4, and hc-MYC, the combination commonly used to create...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ezashi T,Telugu BP,Alexenko AP,Sachdev S,Sinha S,Roberts RM

更新日期：2009-07-07 00:00:00

abstract：:The cDNA of the gene pds from tomato, encoding the carotenoid biosynthesis enzyme phytoene desaturase, was cloned, and its nucleotide sequence was determined. Cells of Escherichia coli that expressed the tomato pds gene could convert phytoene to zeta-carotene. This result suggests that one polypeptide, the product of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pecker I,Chamovitz D,Linden H,Sandmann G,Hirschberg J

更新日期：1992-06-01 00:00:00

abstract：:DNA heteroduplexes with single unpaired bases of the four different kinds were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system can reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dohet C,Wagner R,Radman M

更新日期：1986-05-01 00:00:00

abstract：:A nonselective ex vivo assay was used to directly detect and quantify zidovudine (AZT)-resistant human immunodeficiency virus type 1 (HIV-1) in the blood of treated and untreated patients. In contrast to previous reports, drug-resistant virus was detected in peripheral blood mononuclear cells of a few of the patients ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohri H,Singh MK,Ching WT,Ho DD

更新日期：1993-01-01 00:00:00

abstract：:Adrenal chromaffin granules contain at least 10 peptides, ranging in size from 3 to 5 kilodaltons, that yield, upon digestion with trypsin, peptides that show specific binding to opiate receptors. All are distinctly different from beta-endorphin. Two of these peptides have been purified to homogeneity and subjected to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kimura S,Lewis RV,Stern AS,Rossier J,Stein S,Udenfriend S

更新日期：1980-03-01 00:00:00

abstract：:In response to vascular injury, vascular smooth muscle cells (VSMCs) may switch from a contractile to a proliferative phenotype thereby contributing to neointima formation. Previous studies showed that the long noncoding RNA (lncRNA) NEAT1 is critical for paraspeckle formation and tumorigenesis by promoting cell proli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahmed ASI,Dong K,Liu J,Wen T,Yu L,Xu F,Kang X,Osman I,Hu G,Bunting KM,Crethers D,Gao H,Zhang W,Liu Y,Wen K,Agarwal G,Hirose T,Nakagawa S,Vazdarjanova A,Zhou J

更新日期：2018-09-11 00:00:00

abstract：:A coarse-grained model for protein-folding dynamics is introduced based on a discretized representation of torsional modes. The model, based on the Ramachandran map of the local torsional potential surface and the class (hydrophobic/polar/neutral) of each residue, recognizes patterns of both torsional conformations an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernández A,Kostov K,Berry RS

更新日期：1999-11-09 00:00:00

abstract：:Transcriptionally silent genes are maintained in inaccessible chromatin. Accessibility of these genes requires their modification by chromatin remodeling complexes (CRCs), which are recruited to promoters by sequence-specific DNA-binding proteins. Early B-cell factor (EBF), which is crucial for B-cell lineage specific...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao H,Lukin K,Ramírez J,Fields S,Lopez D,Hagman J

更新日期：2009-07-07 00:00:00

abstract：:The androgen receptor (AR) plays an essential role in prostate cancer, and suppression of its signaling with androgen deprivation therapy (ADT) has been the mainstay of treatment for metastatic hormone-sensitive prostate cancer for more than 70 y. Chemotherapy has been reserved for metastatic castration-resistant pros...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Komura K,Jeong SH,Hinohara K,Qu F,Wang X,Hiraki M,Azuma H,Lee GS,Kantoff PW,Sweeney CJ

更新日期：2016-05-31 00:00:00

abstract：:The crystal structure of the decanucleotide d(CGCAATTGCG)2 has been solved by a combination of molecular replacement and heavy-atom procedures and has been refined to an R factor of 20.2% at 2.7 A. It is not a fully base-paired duplex but has a central core of eight Watson-Crick base pairs flanked by unpaired terminal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spink N,Nunn CM,Vojtechovsky J,Berman HM,Neidle S

更新日期：1995-11-07 00:00:00

abstract：:Autopsy studies suggest that most aging men will develop lesions that, if detected clinically, would be diagnosed as prostate cancer (PCa). Most of these cancers are indolent and remain localized; however, a subset of PCa is aggressive and accounts for more than 27,000 deaths in the United States annually. Identificat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Zheng SL,Isaacs SD,Wiley KE,Wiklund F,Sun J,Kader AK,Li G,Purcell LD,Kim ST,Hsu FC,Stattin P,Hugosson J,Adolfsson J,Walsh PC,Trent JM,Duggan D,Carpten J,Grönberg H,Isaacs WB

更新日期：2010-02-02 00:00:00

abstract：:Mitogen-stimulated scheduled DNA synthesis and DNA excision repair in human lymphocytes, as well as DNA polymerase a activity in a cell-free system, were inhibited by an electrophilic metabolite of benzo[a]pyrene. This metabolite, (+/-)-anti-(7r,8t)-dihydroxy-(9,10t)-epoxy-7,8,9,10-tetrahyd robenzo[a]pyrene (BPDE), co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busbee DL,Joe CO,Norman JO,Rankin PW

更新日期：1984-09-01 00:00:00

abstract：:Signal peptides of membrane proteins are cleaved by endoplasmic reticulum-resident signal peptidase, and thus, are not present on mature membrane proteins. Here, we report that, contrary to the paradigm, the signal peptide of ruminant CD18, the beta-subunit of beta(2)-integrins, is not cleaved. Intriguingly, the intac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shanthalingam S,Srikumaran S

更新日期：2009-09-08 00:00:00

abstract：:Some cells from cultured embryonic mouse hypothalamus were found to express aromatic-L-amino acid decarboxylase (EC 4.1.1.28) activity and serotonin uptake and storage. These neuron-like cells differed from serotoninergic neurons in cultured embryonic mouse brain stem since they did not contain tryptophan hydroxylase....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Vitry F,Hamon M,Catelon J,Dubois M,Thibault J

更新日期：1986-11-01 00:00:00

abstract：:A central question in protein-DNA recognition is the origin of the specificity that permits binding to the correct site in the presence of excess, nonspecific DNA. In the P22 Arc repressor, the Phe-10 side chain is part of the hydrophobic core of the free protein but rotates out to pack against the sugar-phosphate bac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schildbach JF,Karzai AW,Raumann BE,Sauer RT

更新日期：1999-02-02 00:00:00

abstract：:We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joho R,Weissman IL,Early P,Cole J,Hood L

更新日期：1980-02-01 00:00:00

abstract：:Using an expression cloning approach, we have isolated a cDNA encoding GD3 synthase (CMP-NeuAc:NeuAc alpha 2-3Gal beta 1-4Glc beta 1-1'Cer alpha 2,8-sialyltransferase, EC 2.4.99.8), which is a key regulatory enzyme determining the prominence of the ganglioside biosynthesis pathway. The cloned cDNA encodes a 341-amino ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nara K,Watanabe Y,Maruyama K,Kasahara K,Nagai Y,Sanai Y

更新日期：1994-08-16 00:00:00

abstract：:We present molecular dating analyses for land plants that incorporate 33 fossil calibrations, permit rates of molecular evolution to be uncorrelated across the tree, and take into account uncertainties in phylogenetic relationships and the fossil record. We attached a prior probability to each fossil-based minimum age...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith SA,Beaulieu JM,Donoghue MJ

更新日期：2010-03-30 00:00:00

abstract：:Allostatic load (AL) is a measure of overall physiological wear-and-tear over the life course, which could partially be the consequence of early life exposures. AL could allow a better understanding of the potential biological pathways playing a role in the construction of the social gradient in adult health. To explo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barboza Solís C,Kelly-Irving M,Fantin R,Darnaudéry M,Torrisani J,Lang T,Delpierre C

更新日期：2015-02-17 00:00:00

abstract：:T4 polynucleotide ligase efficiently catalyzes the head-to-tail joining of the ribo-oligoadenylates, r-(pA)(8) and r-(pA)(10), in the presence of high molecular weight deoxypolythymidylate. The enzyme also catalyzes the joining of deoxy-oligothymidylates, e.g., d-(pT)(10), in the presence of ribopolyadenylate. The enz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kleppe K,Van de Sande JH,Khorana HG

更新日期：1970-09-01 00:00:00

abstract：:Hybrid wheat plants are superior in yield and growth characteristics compared with their homozygous parents. The commercial production of wheat hybrids is difficult because of the inbreeding nature of wheat and the lack of a practical fertility control that enforces outcrossing. We describe a hybrid wheat system that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempe K,Rubtsova M,Gils M

更新日期：2014-06-24 00:00:00

abstract：:Sulfolobus turreted icosahedral virus (STIV) was isolated in acidic hot springs where it infects the archeon Sulfolobus solfataricus. We determined the STIV structure using near-atomic resolution electron microscopy and X-ray crystallography allowing tracing of structural polypeptide chains and visualization of transm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Veesler D,Ng TS,Sendamarai AK,Eilers BJ,Lawrence CM,Lok SM,Young MJ,Johnson JE,Fu CY

更新日期：2013-04-02 00:00:00

abstract：:Recent genome-wide nucleosome mappings along with bioinformatics studies have confirmed that the DNA sequence plays a more important role in the collective organization of nucleosomes in vivo than previously thought. Yet in living cells, this organization also results from the action of various external factors like D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milani P,Chevereau G,Vaillant C,Audit B,Haftek-Terreau Z,Marilley M,Bouvet P,Argoul F,Arneodo A

更新日期：2009-12-29 00:00:00