Abstract:
:We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable and it has the same organization in sperm and embryo DNAs. There are several variable region genes in sperm and embryo that are related to the Vk167 gene. The organization of the V genes in sperm and embryo DNAs is identical. These results show that there is no rearrangement of variable region genes (or "minigenes") during early embryogenesis.
journal_name
Proc Natl Acad Sci U S Aauthors
Joho R,Weissman IL,Early P,Cole J,Hood Ldoi
10.1073/pnas.77.2.1106subject
Has Abstractpub_date
1980-02-01 00:00:00pages
1106-10issue
2eissn
0027-8424issn
1091-6490journal_volume
77pub_type
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