Abstract:
:T4 polynucleotide ligase efficiently catalyzes the head-to-tail joining of the ribo-oligoadenylates, r-(pA)(8) and r-(pA)(10), in the presence of high molecular weight deoxypolythymidylate. The enzyme also catalyzes the joining of deoxy-oligothymidylates, e.g., d-(pT)(10), in the presence of ribopolyadenylate. The enzyme failed to bring about the joining of r-(pA)(10) when poly r-U was used as the template, although a slow formation of the expected activated intermediate from r-(pA)(10) was detected.
journal_name
Proc Natl Acad Sci U S Aauthors
Kleppe K,Van de Sande JH,Khorana HGdoi
10.1073/pnas.67.1.68subject
Has Abstractpub_date
1970-09-01 00:00:00pages
68-73issue
1eissn
0027-8424issn
1091-6490journal_volume
67pub_type
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