Selective growth in serum-free hormone-supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung.

abstract：:Cocaine addiction and overdose have long defied specific treatment. To provide a new approach, the high-activity catalytic antibody mAb 15A10 was elicited using a transition-state analog for the hydrolysis of cocaine to nontoxic, nonaddictive products. In a model of cocaine overdose, mAb 15A10 protected rats from coca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mets B,Winger G,Cabrera C,Seo S,Jamdar S,Yang G,Zhao K,Briscoe RJ,Almonte R,Woods JH,Landry DW

更新日期：1998-08-18 00:00:00

abstract：:The torI gene has been identified by using a genetic multicopy approach as a negative regulator of the torCAD operon that encodes the trimethylamine N-oxide reductase respiratory system in Escherichia coli. The negative effect was due to a previously unidentified small ORF (66 aa) of phage origin that we called torI f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ansaldi M,Théraulaz L,Méjean V

更新日期：2004-06-22 00:00:00

abstract：:Eukaryotic cells require N-linked carbohydrates for survival. However, the biosynthetic intermediate Man5GlcNAc2Asn, in place of mature N-linked structures, allows glycoprotein synthesis and somatic cell growth to proceed normally. To determine whether the same would be true in a complex biological situation, the gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ioffe E,Stanley P

更新日期：1994-01-18 00:00:00

abstract：:Land-use change is a major driver of biodiversity loss worldwide. Although biodiversity often shows a delayed response to land-use change, previous studies have typically focused on a narrow range of current landscape factors and have largely ignored the role of land-use history in shaping plant and animal communities...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le Provost G,Badenhausser I,Le Bagousse-Pinguet Y,Clough Y,Henckel L,Violle C,Bretagnolle V,Roncoroni M,Manning P,Gross N

更新日期：2020-01-21 00:00:00

abstract：:Microtubules (MTs) are hollow cytoskeletal filaments assembled from αβ-tubulin heterodimers. Tau, an unstructured protein found in neuronal axons, binds to MTs and regulates their dynamics. Aberrant Tau behavior is associated with neurodegenerative dementias, including Alzheimer's. Here, we report on a direct force me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung PJ,Choi MC,Miller HP,Feinstein HE,Raviv U,Li Y,Wilson L,Feinstein SC,Safinya CR

更新日期：2015-11-24 00:00:00

abstract：:Metallophilicity is defined as the interaction among closed-shell metal centers, the origin of which remains controversial, particularly for the roles of spd orbital hybridization (mixing of the spd atomic orbitals of the metal atom in the molecular orbitals of metal complex) and the relativistic effect. Our studies r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wan Q,Yang J,To WP,Che CM

更新日期：2021-01-05 00:00:00

abstract：:The [A] allele of SNP rs965513 in 9q22 has been consistently shown to be highly associated with increased papillary thyroid cancer (PTC) risk with an odds ratio of ∼1.8 as determined by genome-wide association studies, yet the molecular mechanisms remain poorly understood. Previously, we noted that the expression of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He H,Li W,Liyanarachchi S,Srinivas M,Wang Y,Akagi K,Wang Y,Wu D,Wang Q,Jin V,Symer DE,Shen R,Phay J,Nagy R,de la Chapelle A

更新日期：2015-05-12 00:00:00

abstract：:The dissociation and association of an immobilized preparation of the octameric enzyme porphobilinogen synthase [5-aminolevulinate hydro-lyase (adding 5-aminolevulinate and cyclizing), EC 4.2.1.24] is described. On treatment of the immobilized preparation with 4 M urea, four subunits per octamer are removed which can ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gurne D,Chen J,Shemin D

更新日期：1977-04-01 00:00:00

abstract：:Granulocyte-colony stimulating factor (G-CSF) induces differentiation of M1 murine myeloid leukemia cells into mature granulocytes/macrophages and also causes accumulation of the cells in the G1 phase of the cell cycle. We examined, therefore, whether synchronization of M1 cells in the G1 phase could affect G-CSF-indu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsuda H,Neckers LM,Pluznik DH

更新日期：1986-06-01 00:00:00

abstract：:The neural crest (NC) emerges from combinatorial inductive events occurring within its progenitor domain, the neural border (NB). Several transcription factors act early at the NB, but the initiating molecular events remain elusive. Recent data from basal vertebrates suggest that ap2 might have been critical for NC em...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Crozé N,Maczkowiak F,Monsoro-Burq AH

更新日期：2011-01-04 00:00:00

abstract：:The tumorigenic properties of Rous sarcoma virus are attributable to a 60,000-dalton protein, pp60v--src, encoded by a single viral gene, v-src. A homologous gene, c-src, that contains the information for a 60,000-dalton protein, pp60c--src, has been identified in all tested vertebrate cells. By screening a recombinan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker RC,Varmus HE,Bishop JM

更新日期：1981-09-01 00:00:00

abstract：:An acid extract of pedal ganglia of the mollusc Mytilus edulis was fractionated by high-pressure liquid chromatography with a reverse-phase column. Peak fractions with retention times of those of [Met]- and [Leu]enkephalin were subjected to binding assays in both invertebrate and vertebrate tissues. The results showed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung MK,Stefano GB

更新日期：1984-02-01 00:00:00

abstract：:Plasmons, the collective oscillations of interacting electrons, possess emergent properties that dramatically alter the optical response of metals. We predict the existence of a new class of plasmons-chiral Berry plasmons (CBPs)-for a wide range of 2D metallic systems including gapped Dirac materials. As we show, in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song JC,Rudner MS

更新日期：2016-04-26 00:00:00

abstract：:Acidification of the extracellular milieu of malignant tumors is reported to increase the invasive behavior of cancer cells. In normal tissues, production of acid is catalyzed by carbonic anhydrases (CAs), some of which are known to be overexpressed in certain cancers. To investigate the functional role of CA activity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parkkila S,Rajaniemi H,Parkkila AK,Kivela J,Waheed A,Pastorekova S,Pastorek J,Sly WS

更新日期：2000-02-29 00:00:00

abstract：:Noncompetitive inhibitors of the nicotinic acetylcholine (ACh) receptors suppress cation flux directly by binding in and blocking the open channel or indirectly by stabilizing closed states of the receptor. The lidocaine derivative QX-314 and the acridine derivative quinacrine act directly as open channel blockers, bu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Y,Shi L,Karlin A

更新日期：2003-04-01 00:00:00

abstract：:Human cancer cells that had had high (greater than 160) tissue culture passages, when transplanted into antithymocyte-treated F344 newborn rats, caused induction of rat sarcomas in the rats within 2 or 3 subcultures, whereas human cancer cells with low (5-33) passages in vitro did not cause overt induction of rat sarc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huebner RJ,Fish DC,Djurickovic D,Trimmer RW,Bare AL,Bare RM,Smith GT

更新日期：1979-04-01 00:00:00

abstract：:Many experiments in the past have demonstrated the requirement of de novo gene expression during the long-term retention of learning and memory. Although previous studies implicated individual genes or genetic pathways in learning and memory, they did not uncover the collective behaviors or patterns of the genes. We h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cavallaro S,D'Agata V,Manickam P,Dufour F,Alkon DL

更新日期：2002-12-10 00:00:00

abstract：:The discovery of RNAi has revolutionized loss-of-function genetic studies in mammalian systems. However, significant challenges still remain to fully exploit RNAi for mammalian genetics. For instance, genetic screens and in vivo studies could be broadly improved by methods that allow inducible and uniform gene express...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meerbrey KL,Hu G,Kessler JD,Roarty K,Li MZ,Fang JE,Herschkowitz JI,Burrows AE,Ciccia A,Sun T,Schmitt EM,Bernardi RJ,Fu X,Bland CS,Cooper TA,Schiff R,Rosen JM,Westbrook TF,Elledge SJ

更新日期：2011-03-01 00:00:00

abstract：:Gene expression has a stochastic component because of the single-molecule nature of the gene and the small number of copies of individual DNA-binding proteins in the cell. We show how the statistics of such systems can be mapped onto quantum many-body problems. The dynamics of a single gene switch resembles the spin-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasai M,Wolynes PG

更新日期：2003-03-04 00:00:00

abstract：:Novel antibiotics are urgently needed to combat multidrug-resistant pathogens. Venoms represent previously untapped sources of novel drugs. Here we repurposed mastoparan-L, the toxic active principle derived from the venom of the wasp Vespula lewisii, into synthetic antimicrobials. We engineered within its N terminus ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silva ON,Torres MDT,Cao J,Alves ESF,Rodrigues LV,Resende JM,Lião LM,Porto WF,Fensterseifer ICM,Lu TK,Franco OL,de la Fuente-Nunez C

更新日期：2020-10-27 00:00:00

abstract：:The POU-IV or "Brn-3" class of POU-domain transcription factors is represented in Drosophila by I-POU and twin-of-I-POU, alternative splice products of the I-POU gene. I-POU has been previously reported to inhibit DNA binding by the POU-III class factor drifter/Cf1a via the formation of heterodimeric complexes. Here w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turner EE

更新日期：1996-12-24 00:00:00

abstract：:Expansion of myeloid cells associated with solid tumor development is a key contributor to neoplastic progression. Despite their clinical relevance, the mechanisms controlling myeloid cell production and activity in cancer remains poorly understood. Using a multistage mouse model of breast cancer, we show that product...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casbon AJ,Reynaud D,Park C,Khuc E,Gan DD,Schepers K,Passegué E,Werb Z

更新日期：2015-02-10 00:00:00

abstract：:Recent results suggested that a large subset of heat shock protein HSP-60 reactive peripheral lymphoid gamma delta T cells preexists in normal adult mice, all members of which respond to a single segment of this common HSP. However, the experimental evidence supporting this idea involved in vitro peptide responses of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu YX,Cranfill R,Vollmer M,Van Der Zee R,O'Brien RL,Born W

更新日期：1993-01-01 00:00:00

abstract：:The process by which cells select one of several specific programs of genetic information in development was examined in homoeotic mutant Antennapedia of Rappaport in Drosophila melanogaster. The results show that the alteration of determination from antenna to leg induced by this homoeotic mutation occurs in a popula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Postlethwait JH,Schneiderman HA

更新日期：1969-09-01 00:00:00

abstract：:Investigations into the biophysical properties of single molecules traditionally involve well defined in vitro systems where parameters such as solvent viscosity and applied forces are known a priori. These systems provide means to develop models describing the polymers response to a variety of conditions, including t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fisher JK,Ballenger M,O'Brien ET,Haase J,Superfine R,Bloom K

更新日期：2009-06-09 00:00:00

abstract：:E. coli cells treated with the bifunctional crosslinking reagents dimethyl malonimidate, succinimidate, adipimidate, suberimidate, and sebacinimidate served for the isolation of rod-shaped "ghosts." These ghosts proved to be crosslinked over their entire surface; i.e., a macromolecule (resistant to boiling 1% Na dodec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haller I,Henning U

更新日期：1974-05-01 00:00:00

abstract：:The prefrontal cortex plays a fundamental role in the working memory functions of the cerebral cortex and is also the site of dysfunction in several neurological and psychiatric disorders, including schizophrenia. Prefrontal neurons are distinguished by their capacity for sustained activity during the time a stimulus ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao WJ,Krimer LS,Goldman-Rakic PS

更新日期：2001-01-02 00:00:00

abstract：:Mice lacking major histocompatibility complex (MHC) antigens were generated by mating beta 2-microglobulin-deficient, and therefore class I-deficient, animals with MHC class II-deficient animals. When housed under sterile conditions, the resulting MHC-deficient mice appear healthy, survive for many months, and breed s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grusby MJ,Auchincloss H Jr,Lee R,Johnson RS,Spencer JP,Zijlstra M,Jaenisch R,Papaioannou VE,Glimcher LH

更新日期：1993-05-01 00:00:00

abstract：:"Evolution behaves like a tinkerer" (François Jacob, Science, 1977). Software systems provide a singular opportunity to understand biological processes using concepts from network theory. The Debian GNU/Linux operating system allows us to explore the evolution of a complex network in a unique way. The modular design d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fortuna MA,Bonachela JA,Levin SA

更新日期：2011-12-13 00:00:00

abstract：:mRNA localization is a complex pathway. Besides mRNA sorting per se, this process includes aspects of regulated translation. It requires protein factors that interact with defined sequences (or sequence motifs) of the transcript, and the protein/RNA complexes are finally guided along the cytoskeleton to their ultimate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohr E,Prakash N,Vieluf K,Fuhrmann C,Buck F,Richter D

更新日期：2001-06-19 00:00:00