Abstract:
:Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) was found to increase the adherence of purified peripheral blood monocytes to plastic surfaces and to monolayers of human umbilical vein endothelial cells. With plastic surfaces as a model 9-hr culture with GM-CSF was necessary for enhancement, and maximum levels were obtained after 24-hr stimulation. GM-CSF-stimulated adherence must require new RNA and protein synthesis because actinomycin D and cycloheximide abolished existing adherence and prevented further monocyte attachment. Interestingly, shorter incubations (1-2 hr) with cycloheximide increased adherence, suggesting a labile inhibitor. Formaldehyde fixation of monocytes but not of human vein endothelial cells abolished adherence, indicating the need for actively metabolizing monocytes. Thus, a hemopoietic growth factor, responsible for the proliferation and differentiation of monocytes, can also alter their adhesive characteristics. These observations may have important implications in pathological situations and in the in vivo use of GM-CSF.
journal_name
Proc Natl Acad Sci U S Aauthors
Gamble JR,Elliott MJ,Jaipargas E,Lopez AF,Vadas MAdoi
10.1073/pnas.86.18.7169subject
Has Abstractpub_date
1989-09-01 00:00:00pages
7169-73issue
18eissn
0027-8424issn
1091-6490journal_volume
86pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1982-04-01 00:00:00