Abstract:
:Hybrid wheat plants are superior in yield and growth characteristics compared with their homozygous parents. The commercial production of wheat hybrids is difficult because of the inbreeding nature of wheat and the lack of a practical fertility control that enforces outcrossing. We describe a hybrid wheat system that relies on the expression of a phytotoxic barnase and provides for male sterility. The barnase coding information is divided and distributed at two loci that are located on allelic positions of the host chromosome and are therefore "linked in repulsion." Functional complementation of the loci is achieved through coexpression of the barnase fragments and intein-mediated ligation of the barnase protein fragments. This system allows for growth and maintenance of male-sterile female crossing partners, whereas the hybrids are fertile. The technology does not require fertility restorers and is based solely on the genetic modification of the female crossing partner.
journal_name
Proc Natl Acad Sci U S Aauthors
Kempe K,Rubtsova M,Gils Mdoi
10.1073/pnas.1402836111subject
Has Abstractpub_date
2014-06-24 00:00:00pages
9097-102issue
25eissn
0027-8424issn
1091-6490pii
1402836111journal_volume
111pub_type
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