Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands.

abstract：:Nuclear import of classical nuclear localization sequence-bearing proteins is mediated by karyopherin alpha/beta1 heterodimers. A second nuclear import pathway, mediated by karyopherin beta2 (transportin), recently was described for mRNA-binding proteins. Here we report the cloning and characterization of human karyop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaseen NR,Blobel G

更新日期：1997-04-29 00:00:00

abstract：:Memory is stored in neural networks via changes in synaptic strength mediated in part by NMDA receptor (NMDAR)-dependent long-term potentiation (LTP). Here we show that a cholecystokinin (CCK)-B receptor (CCKBR) antagonist blocks high-frequency stimulation-induced neocortical LTP, whereas local infusion of CCK induces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Li X,Wong YT,Zheng X,Wang H,Peng Y,Feng H,Feng J,Baibado JT,Jesky R,Wang Z,Xie H,Sun W,Zhang Z,Zhang X,He L,Zhang N,Zhang Z,Tang P,Su J,Hu LL,Liu Q,He X,Tan A,Sun X,Li M,Wong K,Wang X,Cheung

更新日期：2019-03-26 00:00:00

abstract：:Perception can be described as a process of inference, integrating bottom-up sensory inputs and top-down expectations. However, it is unclear how this process is neurally implemented. It has been proposed that expectations lead to prestimulus baseline increases in sensory neurons tuned to the expected stimulus, which ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kok P,Mostert P,de Lange FP

更新日期：2017-09-26 00:00:00

abstract：:In this note the Weierstrass integral J is taken as L, the Riemann integral of length. Here M(n), n > 1, is a compact, connected manifold of class C(infinity) with a positive definite Riemann structure. Presentations (phi, U(phi)) in M(n) and geodesics (with the aid of the Euler-Riemann equations) are defined in Morse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morse M

更新日期：1977-06-01 00:00:00

abstract：:Many cortical neurons combine the information ascending and descending the cortical hierarchy. In the classical view, this information is combined nonlinearly to give rise to a single firing-rate output, which collapses all input streams into one. We analyze the extent to which neurons can simultaneously represent mul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Naud R,Sprekeler H

更新日期：2018-07-03 00:00:00

abstract：:Ammonia-oxidizing archaea are ubiquitous in marine and terrestrial environments and now thought to be significant contributors to carbon and nitrogen cycling. The isolation of Candidatus "Nitrosopumilus maritimus" strain SCM1 provided the opportunity for linking its chemolithotrophic physiology with a genomic inventor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker CB,de la Torre JR,Klotz MG,Urakawa H,Pinel N,Arp DJ,Brochier-Armanet C,Chain PS,Chan PP,Gollabgir A,Hemp J,Hügler M,Karr EA,Könneke M,Shin M,Lawton TJ,Lowe T,Martens-Habbena W,Sayavedra-Soto LA,Lang D,Sieve

更新日期：2010-05-11 00:00:00

abstract：:Human platelets stimulated by epinephrine undergo enhanced turnover of phosphatidylinositol 4,5-bisphosphate, accumulate inositol trisphosphate, diacylglycerol, and phosphatidic acid, and phosphorylate a 47-kDa protein. All of these phenomena indicate stimulation of phospholipase C. These responses are blocked complet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banga HS,Simons ER,Brass LF,Rittenhouse SE

更新日期：1986-12-01 00:00:00

abstract：:We noticed that disease-related amyloidogenic proteins and especially cellular prion proteins have the highest proportion of incompletely desolvated backbone H bonds among soluble proteins. Such bonds are vulnerable to water attack and thus represent structural weaknesses. We have measured the adsorption of proteins o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernández A,Berry RS

更新日期：2003-03-04 00:00:00

abstract：:Cultures were prepared of embryonic cells from Drosophila melanogaster. Neurons and myocytes differentiated in vitro from their respective stem cells. Electron microscopy showed that neuromuscular junctions formed where axons contacted myocytes. Electrical stimuli were applied to axons and these caused contractions of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seecof RL,Teplitz RL,Gerson I,Ikeda K,Donady J

更新日期：1972-03-01 00:00:00

abstract：:The pore domain of voltage-gated potassium (Kv) channels consists of transmembrane helices S5 and S6, the turret, the pore helix, the selectivity filter, and the loop preceding S6, with a tertiary reentrant structure between S5 and S6. Using biogenic intermediates, mass tagging (pegylation), and a molecular tape measu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gajewski C,Dagcan A,Roux B,Deutsch C

更新日期：2011-02-22 00:00:00

abstract：:Seismic magnitudes determined from surface and body waves for the Soviet underground nuclear explosion of September 14, 1988, are used to calculate the yield of that event from previously derived calibration curves. The yield obtained by combining the two seismic estimates is 113 kilotons, which is very close to those...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sykes LR,Ekström G

更新日期：1989-05-01 00:00:00

abstract：:Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to immobilize proteins with a single or a few thiol groups via disulfide bridges. The immobilized proteins were subsequently proteolytically degraded. After washing, the thiol-containing peptides were eluted with a reducing a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egorov TA,Svenson A,Rydén L,Carlsson J

更新日期：1975-08-01 00:00:00

abstract：:Escherichia coli K12 2320(lambda)-15B has a mutation that results in ochre suppressor activity.(1) This mutation concomitantly causes a decreased growth rate in rich medium, an increased sensitivity to streptomycin,(1) and the production of some altered 30S ribosomes which are differentially sensitive to RNase.(2) The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gartner TK,Orias E,Lannan JE,Beeson J,Reid PJ

更新日期：1969-03-01 00:00:00

abstract：:The 24 human chromosome types of normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophoto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrano AV,Gray JW,Langlois RG,Burkhart-Schultz KJ,Van Dilla MA

更新日期：1979-03-01 00:00:00

abstract：:Developing tools for investigating the cellular activity of glycans will help to delineate the molecular basis for aberrant glycosylation in pathological processes such as cancer. Metabolic oligosaccharide engineering, which inserts sugar-reporting groups into cellular glycoconjugates, represents a powerful method for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu TL,Hanson SR,Kishikawa K,Wang SK,Sawa M,Wong CH

更新日期：2007-02-20 00:00:00

abstract：:Sulfated glycosaminoglycans (GAGs) such as heparan sulfate (HS) are heteropolysaccharides implicated in the pathology of protein aggregation diseases including localized and systemic forms of amyloidosis. Among subdomains of sulfated GAGs, highly sulfated domains of HS, called HS S-domains, have been highlighted as be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iwahashi N,Ikezaki M,Nishikawa T,Namba N,Ohgita T,Saito H,Ihara Y,Shimanouchi T,Ino K,Uchimura K,Nishitsuji K

更新日期：2020-12-29 00:00:00

abstract：:Avian leukosis virus subgroup J (ALV-J) is an important concern for the poultry industry. Replication of ALV-J depends on a functional cellular receptor, the chicken Na+/H+ exchanger type 1 (chNHE1). Tryptophan residue number 38 of chNHE1 (W38) in the extracellular portion of this molecule is a critical amino acid for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koslová A,Trefil P,Mucksová J,Reinišová M,Plachý J,Kalina J,Kučerová D,Geryk J,Krchlíková V,Lejčková B,Hejnar J

更新日期：2020-01-28 00:00:00

abstract：:Optimization theory has been used to analyze evolutionary adaptation. This theory has explained many features of biological systems, from the genetic code to animal behavior. However, these systems show important deviations from optimality. Typically, these deviations are large in some particular components of the sys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Escudero A,Rivera-Alba M,de Polavieja GG

更新日期：2009-12-01 00:00:00

abstract：:Ligand specificity of a murine gammadelta T-cell receptor-expressing hybridoma (KN6) derived from adult thymocytes has been analyzed in detail. The molecule recognized by the KN6 gammadelta T-cell receptor is expressed on syngeneic cells of various sources (peritoneal macrophages, thymocytes, spleen cells, and Abelson...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonneville M,Ito K,Krecko EG,Itohara S,Kappes D,Ishida I,Kanagawa O,Janeway CA,Murphy DB,Tonegawa S

更新日期：1989-08-01 00:00:00

abstract：:Inactivating mutations of the pro-opiomelanocortin (POMC) gene in both mice and humans leads to hyperphagia and obesity. To further examine the mechanisms whereby POMC-deficiency leads to disordered energy homeostasis, we have generated mice lacking all POMC-derived peptides. Consistent with a previously reported mode...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Challis BG,Coll AP,Yeo GS,Pinnock SB,Dickson SL,Thresher RR,Dixon J,Zahn D,Rochford JJ,White A,Oliver RL,Millington G,Aparicio SA,Colledge WH,Russ AP,Carlton MB,O'Rahilly S

更新日期：2004-03-30 00:00:00

abstract：:The capacity of a so-called male recombination (MR) chromosome in Drosophila melanogaster to generate mutations at 14 X-chromosome loci specifying visible phenotypes was investigated. Appreciable increases in mutation were found at three loci y, sn, and ras. Tests of represnetative mutants generated at each locus esta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Green MM

更新日期：1977-08-01 00:00:00

abstract：:Infective stages of the protozoan parasite Leishmania spp. accumulate a class of beta-1,2-mannan oligosaccharides as their major carbohydrate reserve material. Here, we describe the biosynthesis of Leishmania mannan. Mannan precursors were identified by metabolic labeling of Leishmania mexicana promastigotes with [(3)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sernee MF,Ralton JE,Dinev Z,Khairallah GN,O'Hair RA,Williams SJ,McConville MJ

更新日期：2006-06-20 00:00:00

abstract：:We describe an algorithm to compute trisections of orientable four-manifolds using arbitrary triangulations as input. This results in explicit complexity bounds for the trisection genus of a 4-manifold in terms of the number of pentachora (4-simplices) in a triangulation. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bell M,Hass J,Rubinstein JH,Tillmann S

更新日期：2018-10-23 00:00:00

abstract：:We have totally synthesized a gene that codes for rat hepatic cytochrome b5. The 5' flanking region was designed for efficient expression of this gene in Escherichia coli by incorporating an optimum ribosome binding site and spacer region. Both a soluble form, analogous to the protease-treated microsomal protein, as w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beck von Bodman S,Schuler MA,Jollie DR,Sligar SG

更新日期：1986-12-01 00:00:00

abstract：:The plant trans-Golgi network (TGN) is a central trafficking hub where secretory, vacuolar, recycling, and endocytic pathways merge. Among currently known molecular players involved in TGN transport, three different adaptor protein (AP) complexes promote vesicle generation at the TGN with different cargo specificity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heinze L,Freimuth N,Rößling AK,Hahnke R,Riebschläger S,Fröhlich A,Sampathkumar A,McFarlane HE,Sauer M

更新日期：2020-10-13 00:00:00

abstract：:Numerous international bodies have advocated the development of strategies to achieve the sustainability of marine environments. Typically, such strategies are based on information from expert groups about causes of degradation and policy options to address them, but these strategies rarely take into account assessed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gelcich S,Buckley P,Pinnegar JK,Chilvers J,Lorenzoni I,Terry G,Guerrero M,Castilla JC,Valdebenito A,Duarte CM

更新日期：2014-10-21 00:00:00

abstract：:Epilepsy is characterized by recurrent synchronizations of neuronal activity, which are both a cardinal clinical symptom and a debilitating phenomenon. Although the temporal dynamics of epileptiform synchronizations are well described at the macroscopic level using electrophysiological approaches, less is known about ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldt Muldoon S,Soltesz I,Cossart R

更新日期：2013-02-26 00:00:00

abstract：:A hitherto unknown Neoproterozoic orogenic system, the Saharides, is described in North Africa. It formed during the 900-500-Ma interval. The Saharides involved large subduction accretion complexes occupying almost the entire Arabian Shield and much of Egypt and parts of the small Precambrian inliers in the Sahara inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Şengör AMC,Lom N,Zabcı C,Sunal G,Öner T

更新日期：2020-12-22 00:00:00

abstract：:Naked mole-rats form eusocial colonies consisting of a single breeding female (the queen), several breeding males, and sexually immature adults (subordinates). Subordinates are cooperative and provide alloparental care by huddling and retrieving pups to the nest. However, the physiological mechanism(s) underlying allo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watarai A,Arai N,Miyawaki S,Okano H,Miura K,Mogi K,Kikusui T

更新日期：2018-09-11 00:00:00

abstract：:Psoralens linked to triplex-forming oligonucleotides (psoTFOs) have been used in conjunction with laser-induced two-photon excitation (TPE) to damage a specific DNA target sequence. To demonstrate that TPE can initiate photochemistry resulting in psoralen-DNA photoadducts, target DNA sequences were incubated with psoT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oh DH,King BA,Boxer SG,Hanawalt PC

更新日期：2001-09-25 00:00:00