Abstract:
:Escherichia coli K12 2320(lambda)-15B has a mutation that results in ochre suppressor activity.(1) This mutation concomitantly causes a decreased growth rate in rich medium, an increased sensitivity to streptomycin,(1) and the production of some altered 30S ribosomes which are differentially sensitive to RNase.(2) The results presented below demonstrate that the molecules which cause suppression are tRNA. These observations justify the conclusions that the suppressor mutation did not occur in a structural gene for a ribosomal component, and that the decreased growth rate in rich medium, the increased sensitivity to streptomycin, and the production of altered 30S ribosomes are probably all secondary consequences of the suppressor mutation.
journal_name
Proc Natl Acad Sci U S Aauthors
Gartner TK,Orias E,Lannan JE,Beeson J,Reid PJdoi
10.1073/pnas.62.3.946subject
Has Abstractpub_date
1969-03-01 00:00:00pages
946-51issue
3eissn
0027-8424issn
1091-6490journal_volume
62pub_type
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