Abstract:
:The human eosinophil differentiation factor (EDF) gene was cloned from a genomic library in lambda phage EMBL3A by using a murine EDF cDNA clone as a probe. The DNA sequence of a 3.2-kilobase BamHI fragment spanning the gene was determined. The gene contains three introns. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin 5 but shows no significant homology with other known hemopoietic growth regulators. The amino acid sequence shows strong homology (approximately 70% identity) with that of murine EDF. Recombinant human EDF, expressed from the human EDF gene after transfection into monkey COS cells, stimulated the production of eosinophils and eosinophil colonies from normal human bone marrow but had no effect on the production of neutrophils or mononuclear cells (monocytes and lymphoid cells). The apparent specificity of human EDF for the eosinophil lineage in myeloid hemopoiesis contrasts with the properties of human interleukin 3 and granulocyte/macrophage and granulocyte colony-stimulating factors but is directly analogous to the biological properties of murine EDF. Human EDF therefore represents a distinct hemopoietic growth factor that could play a central role in the regulation of eosinophilia.
journal_name
Proc Natl Acad Sci U S Aauthors
Campbell HD,Tucker WQ,Hort Y,Martinson ME,Mayo G,Clutterbuck EJ,Sanderson CJ,Young IGdoi
10.1073/pnas.84.19.6629subject
Has Abstractpub_date
1987-10-01 00:00:00pages
6629-33issue
19eissn
0027-8424issn
1091-6490journal_volume
84pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1996-11-26 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1981-06-01 00:00:00
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更新日期:1985-05-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1981-02-01 00:00:00