The secreted Ipa complex of Shigella flexneri promotes entry into mammalian cells.

Abstract:

:The bacterial pathogen Shigella flexneri causes bacillary dysentery in humans by invading coloncytes. Upon contact with epithelial cells, S. flexneri elicits localized plasma membrane projections sustained by long actin filaments which engulf the microorganism. The products necessary for Shigella entry include three secretory proteins: IpaB, IpaC, and IpaD. Extracellular IpaB and IpaC associate in a soluble complex, the Ipa complex. We have immunopurified this Ipa complex on latex beads and found that they were efficiently internalized into HeLa cells. Like S. flexneri entry, uptake of the beads bearing the Ipa complex was associated with membrane projections and polymerization of actin at the site of cell-bead interaction and was dependent on small Rho GTPases. These results indicate that a secreted factor can promote S. flexneri entry into epithelial cells.

authors

Ménard R,Prévost MC,Gounon P,Sansonetti P,Dehio C

doi

10.1073/pnas.93.3.1254

subject

Has Abstract

pub_date

1996-02-06 00:00:00

pages

1254-8

issue

3

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

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