Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells.

abstract：:In the past 15 years, the adoption of subnational immigration policies in the United States, such as those established by individual states, has gone from nearly zero to over 300 per year. These include welcoming policies aimed at attracting and incorporating immigrants, as well as unwelcoming policies directed at den...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huo YJ,Dovidio JF,Jiménez TR,Schildkraut DJ

更新日期：2018-01-30 00:00:00

abstract：:Activation of naïve cluster of differentiation (CD)8(+) cytotoxic T lymphocytes (CTLs) is a tightly regulated process, and specific dendritic cell (DC) subsets are typically required to activate naive CTLs. Potential pathways for antigen presentation leading to CD8(+) T-cell priming include direct presentation, cross-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Kim S,Herndon JM,Goedegebuure P,Belt BA,Satpathy AT,Fleming TP,Hansen TH,Murphy KM,Gillanders WE

更新日期：2012-07-31 00:00:00

abstract：:The prefrontal cortex (PFC) is assumed to contribute to goal-directed episodic encoding by exerting cognitive control on medial temporal lobe (MTL) memory processes. However, it is thus far unclear to what extent the contribution of PFC-MTL interactions to memory manifests at a structural anatomical level. We combined...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schott BH,Niklas C,Kaufmann J,Bodammer NC,Machts J,Schütze H,Düzel E

更新日期：2011-03-29 00:00:00

abstract：:The electron tunnelling distance between redox enzymes is estimated. The energy transfer mechanism between a pair of charges is described. The dielectric degrees of freedom are incorporated into the electron transfer rate formula. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kemeny G,Mahanti SD

更新日期：1976-08-01 00:00:00

abstract：:By exploiting the cell-cycle-dependent proteolysis of two ubiquitination oscillators, human Cdt1 and geminin, which are the direct substrates of SCF(Skp2) and APC(Cdh1) complexes, respectively, Fucci technique labels mammalian cell nuclei in G(1) and S/G(2)/M phases with different colors. Transgenic mice expressing th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugiyama M,Sakaue-Sawano A,Iimura T,Fukami K,Kitaguchi T,Kawakami K,Okamoto H,Higashijima S,Miyawaki A

更新日期：2009-12-08 00:00:00

abstract：:The mucopolysaccharidoses are a group of genetic diseases characterized by storage of incompletely degraded glycosaminoglycans. Such storage causes marked distortion of many tissues with consequent severe somatic changes and mental retardation. Storage of glycosaminoglycans results from markedly diminished activity of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dorfman A,Matalon R

更新日期：1976-02-01 00:00:00

abstract：:We simulated the behavior of suspended carbon nanotube resonators over a broad range of temperatures to explore the physics of semiflexible polymers in underdamped environments. We find that thermal fluctuations induce strong coupling between resonance modes. This effect leads to spectral fluctuations that readily acc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnard AW,Sazonova V,van der Zande AM,McEuen PL

更新日期：2012-11-20 00:00:00

abstract：:In a posterior silk gland extract, covalently closed circular (ccc) DNA is in a superhelical state that supports more transcription of fibroin gene than does linear DNA. A HeLa cell extract showed neither the supercoiling activity nor the preference for the transcription of ccc DNA over linear DNA. These activities co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirose S,Suzuki Y

更新日期：1988-02-01 00:00:00

abstract：:Mutations at position 187 in secreted gelsolin enable aberrant proteolysis at the 172-173 and 243-244 amide bonds, affording the 71-residue amyloidogenic peptide deposited in Familial Amyloidosis of Finnish Type (FAF). Thermodynamic comparisons of two different domain 2 constructs were carried out to study possible ef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratnaswamy G,Huff ME,Su AI,Rion S,Kelly JW

更新日期：2001-02-27 00:00:00

abstract：:We present a method for predicting protein folding class based on global protein chain description and a voting process. Selection of the best descriptors was achieved by a computer-simulated neural network trained on a data base consisting of 83 folding classes. Protein-chain descriptors include overall composition, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dubchak I,Muchnik I,Holbrook SR,Kim SH

更新日期：1995-09-12 00:00:00

abstract：:A photoreactive derivative of epidermal growth factor (EGF) has been used to identify and specifically label a membrane receptor for EGF on mouse 3T3 cells. Photoactivable EGF, labeled with 125I, was incubated with 3T3 cells and then photolyzed in situ to generate a nitrene capable of reacting with a wide variety of c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das M,Miyakawa T,Fox CF,Pruss RM,Aharonov A,Herschman HR

更新日期：1977-07-01 00:00:00

abstract：:Components in cerebrospinal fluid that are antigenically related to myelin basic protein have been identified by a technique described recently [Barbarese, E., Braun, P. E. & Carson, J. H. (1977) Proc. Natl. Acad. Sci. USA 74, 3360-3364] involving separating the cerebrospinal fluid proteins by sodium dodecyl sulfate/p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carson JH,Barbarese E,Braun PE,McPherson TA

更新日期：1978-04-01 00:00:00

abstract：:Poliovirus mRNA and mRNA transcribed from vesicular stomatitis virus and reovirus genomes efficiently direct protein synthesis in vivo under experimental conditions where the initiation of host protein synthesis is selectively blocked. The selective blockage of host peptide chain initiation after exposure to hypertoni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nuss DL,Oppermann H,Koch G

更新日期：1975-04-01 00:00:00

abstract：:Inteins are intervening protein sequences that undergo self-excision from a precursor protein with concomitant joining of the flanking sequences. Here, we demonstrate intein trans-splicing in Nicotiana tabacum chloroplasts by using the naturally split Ssp DnaE intein. Trans-splicing occurred whether both intein fragme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chin HG,Kim GD,Marin I,Mersha F,Evans TC Jr,Chen L,Xu MQ,Pradhan S

更新日期：2003-04-15 00:00:00

abstract：:The ability to integrate visual information over space is a fundamental component of human pattern vision. Regardless of whether it is for detecting luminance contrast or for recognizing objects in a cluttered scene, the position of the target in the visual field governs the size of a window within which visual inform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwon M,Liu R

更新日期：2019-02-26 00:00:00

abstract：:The sustainability of irrigated agriculture in many arid and semiarid areas of the world is at risk because of a combination of several interrelated factors, including lack of fresh water, lack of drainage, the presence of high water tables, and salinization of soil and groundwater resources. Nowhere in the United Sta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoups G,Hopmans JW,Young CA,Vrugt JA,Wallender WW,Tanji KK,Panday S

更新日期：2005-10-25 00:00:00

abstract：:Lung cancers caused by activating mutations in the epidermal growth factor receptor (EGFR) are initially responsive to small molecule tyrosine kinase inhibitors (TKIs), but the efficacy of these agents is often limited because of the emergence of drug resistance conferred by a second mutation, T790M. Threonine 790 is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yun CH,Mengwasser KE,Toms AV,Woo MS,Greulich H,Wong KK,Meyerson M,Eck MJ

更新日期：2008-02-12 00:00:00

abstract：:The kinase Cdc2p is a central regulator of entry into and progression through nuclear division during mitosis and meiosis in eukaryotes. Cdc2p is activated at the onset of mitosis by dephosphorylation on tyrosine-15, the phosphorylation status of which is determined mainly by the kinase Wee1p and the phosphatase Cdc25...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami-Tonami Y,Yamada-Namikawa C,Tochigi A,Hasegawa N,Kojima H,Kunimatsu M,Nakanishi M,Murakami H

更新日期：2007-09-11 00:00:00

abstract：:Type VI protein secretion system (T6SS) is important for bacterial competition through contact-dependent killing of competitors. T6SS delivers effectors to neighboring cells and corresponding antagonistic proteins confer immunity against effectors that are delivered by sister cells. Although T6SS has been found in mor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong TG,Ho BT,Yoder-Himes DR,Mekalanos JJ

更新日期：2013-02-12 00:00:00

abstract：:Human cells that suffer mild DNA damage can enter a reversible state of growth arrest known as quiescence. This decision to temporarily exit the cell cycle is essential to prevent the propagation of mutations, and most cancer cells harbor defects in the underlying control system. Here we present a mechanistic mathemat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heldt FS,Barr AR,Cooper S,Bakal C,Novák B

更新日期：2018-03-06 00:00:00

abstract：:alpha-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptors undergo constitutive and ligand-induced internalization that requires dynamin and the clathrin adaptor complex AP-2. We report here that an atypical basic motif within the cytoplasmic tails of AMPA-type glutamate receptors directl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kastning K,Kukhtina V,Kittler JT,Chen G,Pechstein A,Enders S,Lee SH,Sheng M,Yan Z,Haucke V

更新日期：2007-02-20 00:00:00

abstract：:Ten eleven translocation (TET) enzymes (TET1/TET2/TET3) and thymine DNA glycosylase (TDG) play crucial roles in early embryonic and germ cell development by mediating DNA demethylation. However, the molecular mechanisms that regulate TETs/TDG expression and their role in cellular differentiation, including that of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu X,Jin L,Wang X,Luo A,Hu J,Zheng X,Tsark WM,Riggs AD,Ku HT,Huang W

更新日期：2013-10-29 00:00:00

abstract：:Smooth muscle cells use fibronectin to bind to type I and type III collagens but bind to type V collagen by a trypsin-resistant intrinsic glycoconjugate. The binding site on type V collagen is located in the alpha A chain. By using collagen-coated filters in a modified Boyden chamber assay for chemotaxis, it was obser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grotendorst GR,Seppä HE,Kleinman HK,Martin GR

更新日期：1981-06-01 00:00:00

abstract：:In this paper we demonstrate that near infrared Fourier transform Raman spectroscopy provides unprecedented biochemical information about the extent of atherosclerosis in human aorta. In particular, elastin, collagen, cholesterol, cholesterol esters, lipids, carotenoids, and calcium apatite deposits all can be discern...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baraga JJ,Feld MS,Rava RP

更新日期：1992-04-15 00:00:00

abstract：:Mosquitoes are major disease vectors because most species must feed on blood from a vertebrate host to produce eggs. Blood feeding by the vector mosquito Aedes aegypti triggers the release of two neurohormones, ovary ecdysteroidogenic hormone (OEH) and insulin-like peptides (ILPs), which activate multiple processes re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogel KJ,Brown MR,Strand MR

更新日期：2015-04-21 00:00:00

abstract：:The nucleotide sequence of mitochondrial DNA of a cytoplasmic "petite" mutant of Saccharomyces cerevisiae is reported. The DNA has a repeat length of 1060 base pairs and contains a genetic marker (oli-1) for the ATPase proteolipid. The nucleotide sequence reveals the presence of part of the structural gene of the subu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Macino G,Tzagoloff A

更新日期：1979-01-01 00:00:00

abstract：:Antibodies specific for allelic determinants of the cell membrane alloantigen Ly-17 were found to react with genetically determined polymorphic sites on the murine IgG Fc receptor (Fc gamma R). Monoclonal antibodies specific for Ly-17.2 and the Fc gamma R detected identical populations of cells in thymus, spleen, lymp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes KL,Palfree RG,Hammerling U,Morse HC 3rd

更新日期：1985-11-01 00:00:00

abstract：:Aerobic respiration is a fundamental energy-generating process; however, there is cost associated with living in an oxygen-rich environment, because partially reduced oxygen species can damage cellular components. Organisms evolved enzymes that alleviate this damage and protect the intracellular milieu, most notably t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaya A,Gerashchenko MV,Seim I,Labarre J,Toledano MB,Gladyshev VN

更新日期：2015-08-25 00:00:00

abstract：:The Mauer mandible, holotype of Homo heidelbergensis, was found in 1907 in fluvial sands deposited by the Neckar River 10 km southeast of Heidelberg, Germany. The fossil is an important key to understanding early human occupation of Europe north of the Alps. Given the associated mammal fauna and the geological context...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner GA,Krbetschek M,Degering D,Bahain JJ,Shao Q,Falguères C,Voinchet P,Dolo JM,Garcia T,Rightmire GP

更新日期：2010-11-16 00:00:00

abstract：:The nuclear envelope is a unique topological structure formed by lipid membranes in eukaryotic cells. Unlike other membrane structures, the nuclear envelope comprises two concentric membrane shells fused at numerous sites with toroid-shaped pores that impart a "geometric" genus on the order of thousands. Despite the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Torbati M,Lele TP,Agrawal A

更新日期：2016-10-04 00:00:00