Genetic instability in Drosophila melanogaster: De novo induction of putative insertion mutations.

abstract：:Although recent studies have shown that brown adipose tissue (BAT) arises from progenitor cells that also give rise to skeletal muscle, the developmental signals that control the formation of BAT remain largely unknown. Here, we show that brown preadipocytes possess primary cilia and can respond to Hedgehog (Hh) signa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nosavanh L,Yu DH,Jaehnig EJ,Tong Q,Shen L,Chen MH

更新日期：2015-04-21 00:00:00

abstract：:Heat shock protein 90-alpha (Hsp90alpha) is an intracellular molecular chaperone. However, it can also be secreted with the underlying regulatory mechanism remaining far from clear. Here we show that the secreted Hsp90alpha is a C-terminal truncated form and its secretion is regulated by the C-terminal EEVD motif via ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Song X,Zhuo W,Fu Y,Shi H,Liang Y,Tong M,Chang G,Luo Y

更新日期：2009-12-15 00:00:00

abstract：:A topological model of the DNA binding site for the cyclic AMP receptor protein (CRP) is presented. A consensus sequence drawn from the known CRP binding sites has several symmetrical subregions that are spatially resolved onto different faces of the DNA helix. Consideration of available biochemical and genetic data s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Neill MC,Amass K,de Crombrugghe B

更新日期：1981-04-01 00:00:00

abstract：:The time-dependent Schrödinger equation is a cornerstone of quantum physics and governs all phenomena of the microscopic world. However, despite its importance, its origin is still not widely appreciated and properly understood. We obtain the Schrödinger equation from a mathematical identity by a slight generalization...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schleich WP,Greenberger DM,Kobe DH,Scully MO

更新日期：2013-04-02 00:00:00

abstract：:Rap phosphatases are a recently discovered family of protein aspartate phosphatases that dephosphorylate the Spo0F--P intermediate of the phosphorelay, thus preventing sporulation of Bacillus subtilis. They are regulators induced by physiological processes that are antithetical to sporulation. The RapA phosphatase is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Perego M,Hoch JA

更新日期：1996-02-20 00:00:00

abstract：:Phycobilisomes isolated from eight different species of cyanobacteria contain in addition to the light-harvesting phycobiliproteins, a small number of colorless polypeptides with molecular weights higher than those of the chromopolypeptide subunits of the phycobiliproteins. In the phycobilisomes of the species examin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Marsac NT,Cohen-bazire G

更新日期：1977-04-01 00:00:00

abstract：:Adhesive P pili of uropathogenic Escherichia coli were not assembled by a strain that lacks the periplasmic disulfide isomerase DsbA. This defect was mostly attributed to the immunoglobulin-like pilus chaperone PapD, which possesses an unusual intrasheet disulfide bond between the last two beta-strands of its CD4-like...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacob-Dubuisson F,Pinkner J,Xu Z,Striker R,Padmanhaban A,Hultgren SJ

更新日期：1994-11-22 00:00:00

abstract：:Plants have intercellular channels, plasmodesmata (PD), that span the cell wall to enable cell-to-cell transport of micro- and macromolecules. We identified an Arabidopsis thaliana embryo lethal mutant increased size exclusion limit 1 (ise1) that results in increased PD-mediated transport of fluorescent tracers. The i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stonebloom S,Burch-Smith T,Kim I,Meinke D,Mindrinos M,Zambryski P

更新日期：2009-10-06 00:00:00

abstract：:Cells of the craniofacial skeleton are derived from a common mesenchymal progenitor. The regulatory factors that control their differentiation into various cell lineages are unknown. To investigate the biological function of dentin matrix protein 1 (DMP1), an extracellular matrix gene involved in calcified tissue form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narayanan K,Srinivas R,Ramachandran A,Hao J,Quinn B,George A

更新日期：2001-04-10 00:00:00

abstract：:Although the binding change mechanism of rotary catalysis by which F1-ATPase hydrolyzes ATP has been supported by equilibrium, kinetic, and structural observations, many questions concerning the function remain unanswered. Because of the importance of this enzyme, the search for a full understanding of its mechanism i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao YQ,Yang W,Marcus RA,Karplus M

更新日期：2003-09-30 00:00:00

abstract：:The surface density of the triggering receptors responsible for the natural killer (NK)-mediated cytotoxicity is crucial for the ability of NK cells to kill susceptible target cells. In this study, we show that transforming growth factor beta1 (TGFbeta1) down-regulates the surface expression of NKp30 and in part of NK...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castriconi R,Cantoni C,Della Chiesa M,Vitale M,Marcenaro E,Conte R,Biassoni R,Bottino C,Moretta L,Moretta A

更新日期：2003-04-01 00:00:00

abstract：:Depolarization of isolated nerve terminals (synaptosomes) has been shown to stimulate neurotransmitter release and to increase the phosphorylation state of a number of proteins, including synapsin I, in a Ca2+-dependent manner. Synapsin I, a prominent nerve terminal phosphoprotein, interacts with the cytoplasmic surfa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sihra TS,Wang JK,Gorelick FS,Greengard P

更新日期：1989-10-01 00:00:00

abstract：:The vacuolated lens (vl) mouse mutant causes congenital cataracts and neural tube defects (NTDs), with the NTDs being caused by abnormal neural fold apposition and fusion. Our positional cloning of vl indicates these phenotypes result from a deletion mutation in an uncharacterized orphan G protein-coupled receptor (GP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matteson PG,Desai J,Korstanje R,Lazar G,Borsuk TE,Rollins J,Kadambi S,Joseph J,Rahman T,Wink J,Benayed R,Paigen B,Millonig JH

更新日期：2008-02-12 00:00:00

abstract：:Embryogenesis requires the timely and coordinated activation of developmental regulators. It has been suggested that the recently discovered class of histone demethylases (UTX and JMJD3) that specifically target the repressive H3K27me3 modification play an important role in the activation of "bivalent" genes in respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Welstead GG,Creyghton MP,Bilodeau S,Cheng AW,Markoulaki S,Young RA,Jaenisch R

更新日期：2012-08-07 00:00:00

abstract：:In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a 6.7-A cryo-electron m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Villa E,Sengupta J,Trabuco LG,LeBarron J,Baxter WT,Shaikh TR,Grassucci RA,Nissen P,Ehrenberg M,Schulten K,Frank J

更新日期：2009-01-27 00:00:00

abstract：:Anxiety disorders are the most prevalent mental disorders in developed societies. Although roles for the prefrontal cortex, amygdala, hippocampus and mediodorsal thalamus in anxiety disorders are well documented, molecular mechanisms contributing to the functions of these structures are poorly understood. Here we repo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tekinay AB,Nong Y,Miwa JM,Lieberam I,Ibanez-Tallon I,Greengard P,Heintz N

更新日期：2009-03-17 00:00:00

abstract：:We have determined the amino acid sequence of the first constant (C) region domain (C delta 1) and the hinge region of the delta heavy chain of human IgD WAH and also the sequence of the adjacent COOH-terminal portion of the variable (V) region, including the JH region. Together with the sequence of the Fc fragment al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Putnam FW,Takahashi N,Tetaert D,Debuire B,Lin LC

更新日期：1981-10-01 00:00:00

abstract：:Efficient and productive virus infection often requires viral countermeasures that block innate immunity. The IFN-inducible 2',5'-oligoadenylate (2-5A) synthetases (OASs) and ribonuclease (RNase) L are components of a potent host antiviral pathway. We previously showed that murine coronavirus (MHV) accessory protein n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang R,Jha BK,Ogden KM,Dong B,Zhao L,Elliott R,Patton JT,Silverman RH,Weiss SR

更新日期：2013-08-06 00:00:00

abstract：:Cortical blood flow at the level of individual capillaries and the coupling of neuronal activity to flow in capillaries are fundamental aspects of homeostasis in the normal and the diseased brain. To probe the dynamics of blood flow at this level, we used two-photon laser scanning microscopy to image the motion of red...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kleinfeld D,Mitra PP,Helmchen F,Denk W

更新日期：1998-12-22 00:00:00

abstract：:New data are reported on the influence of size on the velocity threshold for real movement. As the length of a narrow stimulus object increases, width remaining constant, the velocity threshold also increases. These findings are discussed in relation to some psychophysical experiments on contrast and some physiologica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mates B,Graham CH

更新日期：1970-03-01 00:00:00

abstract：:The previously described peptide material that reacts with antibodies to gastrin and is found in the central nervous system of various vertebrates is present in only the 100,000 X g pellet of postmortem human cerebral cortical grey matter. This immunoreactive material, extractable in boiling water, is biologically act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robberecht P,Deschodt-Lanckman M,Vanderhaeghen JJ

更新日期：1978-01-01 00:00:00

abstract：:Near-zero-index (NZI) supercoupling, the transmission of electromagnetic waves inside a waveguide irrespective of its shape, is a counterintuitive wave effect that finds applications in optical interconnects and engineering light-matter interactions. However, there is a limited knowledge on the local properties of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liberal I,Lobet M,Li Y,Engheta N

更新日期：2020-09-29 00:00:00

abstract：:Nicks within one strand of the bacteriophage T5 DNA molecule act as " weak points" for a novel kind mechanical breakage that can be utilized for "dissecting" the genome. The products from sheared T5(+) DNA include five unique double-stranded segments of the molecule and various combinations of adjacent segments. These...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hayward GS

更新日期：1974-05-01 00:00:00

abstract：:Aberrant promoter hypermethylation is frequently observed in cancer. The potential for this mechanism to contribute to tumor development depends on whether the genes affected are repressed because of their methylation. Many aberrantly methylated genes play important roles in development and are bivalently marked in ES...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sproul D,Nestor C,Culley J,Dickson JH,Dixon JM,Harrison DJ,Meehan RR,Sims AH,Ramsahoye BH

更新日期：2011-03-15 00:00:00

abstract：:An enzyme that reduces methionine sulfoxide [Met(O)] residues in proteins [peptide Met(O) reductase (MsrA), EC 1.8.4.6; originally identified in Escherichia coli] was purified from bovine liver, and the cDNA encoding this enzyme was cloned and sequenced. The mammalian homologue of E. coli msrA (also called pmsR) cDNA ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moskovitz J,Weissbach H,Brot N

更新日期：1996-03-05 00:00:00

abstract：:The assembly of the three neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key step that leads to exocytotic fusion of synaptic vesicles. In the fully assembled SNARE complex, these three proteins form a coiled-coil ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang B,Kiessling V,Tamm LK

更新日期：2013-11-26 00:00:00

abstract：:The POT1-TPP1 heterodimer, the major telomere-specific single-stranded DNA-binding protein in mammalian cells, protects chromosome ends and contributes to the regulation of telomerase. The recent discovery of telomeric RNA raises the question of how POT1 faithfully binds telomeric ssDNA and avoids illicit RNA binding ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nandakumar J,Podell ER,Cech TR

更新日期：2010-01-12 00:00:00

abstract：:E. coli cells treated with the bifunctional crosslinking reagents dimethyl malonimidate, succinimidate, adipimidate, suberimidate, and sebacinimidate served for the isolation of rod-shaped "ghosts." These ghosts proved to be crosslinked over their entire surface; i.e., a macromolecule (resistant to boiling 1% Na dodec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haller I,Henning U

更新日期：1974-05-01 00:00:00

abstract：:The progesterone receptor (PGR) is a ligand-activated transcription factor with key roles in the regulation of female fertility. Much has been learned of the actions of PGR signaling through the use of pharmacologic inhibitors and genetic manipulation, using mouse mutagenesis. Characterization of rats with a null muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubota K,Cui W,Dhakal P,Wolfe MW,Rumi MA,Vivian JL,Roby KF,Soares MJ

更新日期：2016-04-12 00:00:00

abstract：:In 1989, populations of North American gypsy moth, Lymantria dispar, in seven contiguous northeastern states were severely reduced by a fungal pathogen. Based on morphology, development, and pathology, this organism appeared to be Entomophaga maimaiga. We have now used allozyme and restriction fragment length polymorp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajek AE,Humber RA,Elkinton JS,May B,Walsh SR,Silver JC

更新日期：1990-09-01 00:00:00