当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > PapD chaperone function in pilus biogenesis depends on oxidant and chaperone-like activities of DsbA.

PapD chaperone function in pilus biogenesis depends on oxidant and chaperone-like activities of DsbA.

Abstract：

:Adhesive P pili of uropathogenic Escherichia coli were not assembled by a strain that lacks the periplasmic disulfide isomerase DsbA. This defect was mostly attributed to the immunoglobulin-like pilus chaperone PapD, which possesses an unusual intrasheet disulfide bond between the last two beta-strands of its CD4-like carboxyl-terminal domain. The DsbA-dependent formation of this disulfide bond was critical for PapD's proper folding in vivo. Interestingly, the absence of the disulfide bond did not prevent PapD from folding in vitro or from forming a complex with the pilus adhesin in vitro. We suggest that DsbA maintains nascently translocated PapD in a folding-competent conformation prior to catalyzing disulfide bond formation, acting both as an oxidant and in a chaperone-like fashion. Disulfide bond formation in pilus subunits was also mediated by DsbA even in the absence of PapD. However, the ability of pilus subunits to achieve native-like conformations in vivo depended on PapD. These results suggest that a productive folding pathway for subunits requires sequential interactions with DsbA and the PapD chaperone.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Jacob-Dubuisson F,Pinkner J,Xu Z,Striker R,Padmanhaban A,Hultgren SJ

doi

10.1073/pnas.91.24.11552

subject

Has Abstract

pub_date

1994-11-22 00:00:00

pages

11552-6

issue

24

eissn

0027-8424

issn

1091-6490

journal_volume

91

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Control of lambda repressor synthesis.

    abstract：:Direct measurements of the intracellular level of lambda repressor have been made by a DNA-filter assay and a radioimmune assay. Transcription of cI, the structural gene for repressor, appears to initiate at two different promoters, prm and pre. Promoter pre is activated during the establishment of lysogeny by the act...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.68.9.2185

    authors： Reichardt L,Kaiser AD

    更新日期：1971-09-01 00:00:00

  • Crystal structure of the guanylyl cyclase Cya2.

    abstract：:Cyclic GMP (cGMP) is an important second messenger in eukaryotes. It is formed by guanylyl cyclases (GCs), members of the nucleotidyl cyclases class III, which also comprises adenylyl cyclases (ACs) from most organisms. To date, no structures of eukaryotic GCs are available, and all bacterial class III proteins were f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0808473105

    authors： Rauch A,Leipelt M,Russwurm M,Steegborn C

    更新日期：2008-10-14 00:00:00

  • In vitro incorporation of selenium into tRNAs of Salmonella typhimurium.

    abstract：:Broken-cell preparations of Salmonella typhimurium rapidly incorporated 75Se from 75SeO3(2-) into tRNA by an ATP-dependent process. Selenium incorporation in the presence of 50 microM 75SeO3(2-) (0.8-1 pmol per A260 unit) was enhanced by the selenocysteine precursor, O-acetyl-L-serine (to 3.7 pmol per A260 unit). This...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.16.6341

    authors： Veres Z,Tsai L,Politino M,Stadtman TC

    更新日期：1990-08-01 00:00:00

  • Two-dimensional Raman-terahertz spectroscopy of water.

    abstract：:Two-dimensional Raman-terahertz (THz) spectroscopy is presented as a multidimensional spectroscopy directly in the far-IR regime. The method is used to explore the dynamics of the collective intermolecular modes of liquid water at ambient temperatures that emerge from the hydrogen-bond networks water forming. Two-dime...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1317459110

    authors： Savolainen J,Ahmed S,Hamm P

    更新日期：2013-12-17 00:00:00

  • Functional neuroanatomical double dissociation of mnemonic and executive control processes contributing to working memory performance.

    abstract：:We used event-related functional MRI to investigate the neural bases of two categories of mental processes believed to contribute to performance of an alphabetization working memory task: memory storage and memory manipulation. Our delayed-response tasks required memory for the identity and position-in-the-display of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 临床试验,杂志文章

    doi：10.1073/pnas.96.22.12959

    authors： Postle BR,Berger JS,D'Esposito M

    更新日期：1999-10-26 00:00:00

  • Network dynamics of social influence in the wisdom of crowds.

    abstract：:A longstanding problem in the social, biological, and computational sciences is to determine how groups of distributed individuals can form intelligent collective judgments. Since Galton's discovery of the "wisdom of crowds" [Galton F (1907) Nature 75:450-451], theories of collective intelligence have suggested that t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,随机对照试验

    doi：10.1073/pnas.1615978114

    authors： Becker J,Brackbill D,Centola D

    更新日期：2017-06-27 00:00:00

  • The K+ channel KIR2.1 functions in tandem with proton influx to mediate sour taste transduction.

    abstract：:Sour taste is detected by a subset of taste cells on the tongue and palate epithelium that respond to acids with trains of action potentials. Entry of protons through a Zn(2+)-sensitive proton conductance that is specific to sour taste cells has been shown to be the initial event in sour taste transduction. Whether th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1514282112

    authors： Ye W,Chang RB,Bushman JD,Tu YH,Mulhall EM,Wilson CE,Cooper AJ,Chick WS,Hill-Eubanks DC,Nelson MT,Kinnamon SC,Liman ER

    更新日期：2016-01-12 00:00:00

  • Predictable and efficient carbon sequestration in the North Pacific Ocean supported by symbiotic nitrogen fixation.

    abstract：:The atmospheric and deep sea reservoirs of carbon dioxide are linked via physical, chemical, and biological processes. The last of these include photosynthesis, particle settling, and organic matter remineralization, and are collectively termed the "biological carbon pump." Herein, we present results from a 13-y (1992...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1120312109

    authors： Karl DM,Church MJ,Dore JE,Letelier RM,Mahaffey C

    更新日期：2012-02-07 00:00:00

  • Yeast RNase P: catalytic activity and substrate binding are separate functions.

    abstract：:During tRNA biosynthesis the 5'-leader sequences in precursor tRNAs are removed by the ribonucleoprotein RNase P, an enzyme whose RNA moiety is required for activity. To clarify some aspects of the enzyme mechanism, we examined substrate binding and product formation with mutant precursor tRNAs. Mutations G-1----A or ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.5.1379

    authors： Nichols M,Söll D,Willis I

    更新日期：1988-03-01 00:00:00

  • In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease.

    abstract：:We have developed an in situ hybridization assay capable of detecting enteroviral RNA in myocardial cells, using molecularly cloned coxsackievirus B3 cDNA as a diagnostic probe. Because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group A and B coxsackiev...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.17.6272

    authors： Kandolf R,Ameis D,Kirschner P,Canu A,Hofschneider PH

    更新日期：1987-09-01 00:00:00

  • The integral membrane S-locus receptor kinase of Brassica has serine/threonine kinase activity in a membranous environment and spontaneously forms oligomers in planta.

    abstract：:To gain further insight into the mode of action of S-locus receptor kinase (SRK), a receptor-like kinase involved in the self-incompatibility response in Brassica, different recombinant SRK proteins have been expressed in a membranous environment using the insect cell/baculovirus system. Recombinant SRK proteins exhib...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.070025097

    authors： Giranton JL,Dumas C,Cock JM,Gaude T

    更新日期：2000-03-28 00:00:00

  • Visual motion induces synchronous oscillations in turtle visual cortex.

    abstract：:In mammalian brains, multielectrode recordings during sensory stimulation have revealed oscillations in different cortical areas that are transiently synchronous. These synchronizations have been hypothesized to support integration of sensory information or represent the operation of attentional mechanisms, but their ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.26.12467

    authors： Prechtl JC

    更新日期：1994-12-20 00:00:00

  • Low-barrier hydrogen bond in photoactive yellow protein.

    abstract：:Low-barrier hydrogen bonds (LBHBs) have been proposed to play roles in protein functions, including enzymatic catalysis and proton transfer. Transient formation of LBHBs is expected to stabilize specific reaction intermediates. However, based on experimental results and theoretical considerations, arguments against th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0811882106

    authors： Yamaguchi S,Kamikubo H,Kurihara K,Kuroki R,Niimura N,Shimizu N,Yamazaki Y,Kataoka M

    更新日期：2009-01-13 00:00:00

  • Enteroendocrine precursors differentiate independently of Wnt and form serotonin expressing adenomas in response to active beta-catenin.

    abstract：:Wnt signaling is required for the maintenance of intestinal stem cells and self-renewal of the intestinal epithelium. Intestinal cancers are frequently associated with mutations that activate the Wnt pathway. The role of Wnt signaling on differentiation of lineage-specific precursors in the intestine is not well chara...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702665104

    authors： Wang Y,Giel-Moloney M,Rindi G,Leiter AB

    更新日期：2007-07-03 00:00:00

  • Placental-specific insulin-like growth factor 2 (Igf2) regulates the diffusional exchange characteristics of the mouse placenta.

    abstract：:Restricted fetal growth is associated with postnatal mortality and morbidity and may be directly related to alterations in the capacity of the placenta to supply nutrients. We proposed previously that imprinted genes can regulate nutrient supply by the placenta. Here, we tested the hypothesis that the insulin-like gro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0402508101

    authors： Sibley CP,Coan PM,Ferguson-Smith AC,Dean W,Hughes J,Smith P,Reik W,Burton GJ,Fowden AL,Constância M

    更新日期：2004-05-25 00:00:00

  • Insulin-like growth factors 1 and 2 induce lymphangiogenesis in vivo.

    abstract：:Lymphangiogenesis is an important process that contributes to the spread of cancer. Here we show that insulin-like growth factors 1 (IGF-1) and 2 (IGF-2) induce lymphangiogenesis in vivo. In a mouse cornea assay, IGF-1 and IGF-2 induce lymphangiogenesis as detected with LYVE-1, a specific marker for lymphatic endothel...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0507865102

    authors： Björndahl M,Cao R,Nissen LJ,Clasper S,Johnson LA,Xue Y,Zhou Z,Jackson D,Hansen AJ,Cao Y

    更新日期：2005-10-25 00:00:00

  • Reduced Mg2+ block of N-methyl-D-aspartate receptor-mediated synaptic potentials in developing visual cortex.

    abstract：:Molecular cloning has demonstrated a diversity of artificially expressed N-methyl-D-aspartate (NMDA) receptors, implying a similar diversity of naturally occurring NMDA receptors in situ. Particularly significant was the success in expression of NMDA receptor classes exhibiting various sensitivities to Mg2+ block, a v...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.15.7114

    authors： Kato N,Yoshimura H

    更新日期：1993-08-01 00:00:00

  • Abundant pseudogenes for small nuclear RNAs are dispersed in the human genome.

    abstract：:We have cloned and partially characterized 24 loci from the human genome which are complementary to U1, U2, or U3, the three major species of small nuclear RNA (snRNA) in HeLa cells. When compared to the known U1 (human) and U2 (rat) snRNA sequences, the DNA sequences we report here for the complementary regions from ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.2.810

    authors： Denison RA,Van Arsdell SW,Bernstein LB,Weiner AM

    更新日期：1981-02-01 00:00:00

  • Colloidal gold--low density lipoprotein conjugates as membrane receptor probes.

    abstract：:We have developed a method for conjugating low density lipoproteins (LDL) with colloidal gold. Conjugation, complete after 1 min, occurs by electrostatic adsorption of the LDL to the negatively charged gold particle. Each conjugate consists of approximately eight biologically active LDL molecules clustered around a ce...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.1.368

    authors： Handley DA,Arbeeny CM,Witte LD,Chien S

    更新日期：1981-01-01 00:00:00

  • Emigration of monocyte-derived cells from atherosclerotic lesions characterizes regressive, but not progressive, plaques.

    abstract：:Some monocytes normally take up residence in tissues as sessile macrophages, but others differentiate into migratory cells resembling dendritic cells that emigrate to lymph nodes. In an in vitro model of a vessel wall, lipid mediators lysophosphatidic acid and platelet-activating factor, whose signals are implicated i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0403259101

    authors： Llodrá J,Angeli V,Liu J,Trogan E,Fisher EA,Randolph GJ

    更新日期：2004-08-10 00:00:00

  • X-ray structure of glutathione S-transferase from the malarial parasite Plasmodium falciparum.

    abstract：:GSTs catalyze the conjugation of glutathione with a wide variety of hydrophobic compounds, generally resulting in nontoxic products that can be readily eliminated. In contrast to many other organisms, the malarial parasite Plasmodium falciparum possesses only one GST isoenzyme (PfGST). This GST is highly abundant in t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2333763100

    authors： Fritz-Wolf K,Becker A,Rahlfs S,Harwaldt P,Schirmer RH,Kabsch W,Becker K

    更新日期：2003-11-25 00:00:00

  • Differential effects of perhydrohistrionicotoxin on neurally and iontophoretically evoked endplate currents.

    abstract：:Perhydrohistrionicotoxin, at concentrations of 10(-12)-10(-7) M, depressed the current generated by iontophoretic application of acetylcholine to endplate regions of soleus and extensor digitorum longus muscles of rats. However, no changes in the amplitude or time course of spontaneous miniature endplate potentials or...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.3.1596

    authors： Albuquerque EX,Gage PW

    更新日期：1978-03-01 00:00:00

  • Cation-dependent mannose 6-phosphate receptor contains two internalization signals in its cytoplasmic domain.

    abstract：:The signals required for rapid internalization of the bovine cation-dependent mannose 6-phosphate receptor have been localized to two distinct regions of the 67-amino acid cytoplasmic domain. One signal includes phenylalanine 13 and phenylalanine 18, while the other involves tyrosine 45. The former signal is more pote...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.24.10010

    authors： Johnson KF,Chan W,Kornfeld S

    更新日期：1990-12-01 00:00:00

  • The R1 component of mammalian ribonucleotide reductase has malignancy-suppressing activity as demonstrated by gene transfer experiments.

    abstract：:Our recent studies have shown that deregulated expression of R2, the rate-limiting component of ribonucleotide reductase, enhances transformation and malignant potential by cooperating with activated oncogenes. We now demonstrate that the R1 component of ribonucleotide reductase has tumor-suppressing activity. Stable ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.24.13181

    authors： Fan H,Huang A,Villegas C,Wright JA

    更新日期：1997-11-25 00:00:00

  • Phenotypic conversion of drug-resistant bacteria to drug sensitivity.

    abstract：:Plasmids that contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EGSs) have been introduced into strains of Escherichia coli harboring antibiotic resistance genes. The EGSs direct RNase P to cleave the mRNAs transcribed from these genes thereby converting the phenotype of dr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.16.8468

    authors： Guerrier-Takada C,Salavati R,Altman S

    更新日期：1997-08-05 00:00:00

  • From the Cover: Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering.

    abstract：:With the completion of genome sequences of major model organisms, increasingly sophisticated genetic tools are necessary for investigating the complex and coordinated functions of genes. Here we describe a genetic manipulation system termed "genomic engineering" in Drosophila. Genomic engineering is a 2-step process t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0900641106

    authors： Huang J,Zhou W,Dong W,Watson AM,Hong Y

    更新日期：2009-05-19 00:00:00

  • D-serine, an endogenous synaptic modulator: localization to astrocytes and glutamate-stimulated release.

    abstract：:Using an antibody highly specific for D-serine conjugated to glutaraldehyde, we have localized endogenous D-serine in rat brain. Highest levels of D-serine immunoreactivity occur in the gray matter of the cerebral cortex, hippocampus, anterior olfactory nucleus, olfactory tubercle, and amygdala. Localizations of D-ser...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.9.3948

    authors： Schell MJ,Molliver ME,Snyder SH

    更新日期：1995-04-25 00:00:00

  • Human cytomegalovirus latency alters the cellular secretome, inducing cluster of differentiation (CD)4+ T-cell migration and suppression of effector function.

    abstract：:After primary infection, human cytomegalovirus (HCMV) persists as a life-long latent infection, with host immunosuppression often resulting in clinical reactivation. During lytic infection, major changes in the expression of secreted cellular proteins (the secretome) occur that have profound effects on host-cell inter...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1204836109

    authors： Mason GM,Poole E,Sissons JG,Wills MR,Sinclair JH

    更新日期：2012-09-04 00:00:00

  • N-acetyl-D-galactosaminyltransferase in human serum and erythrocyte membranes.

    abstract：:This study demonstrates the presence of an N-acetyl-D-galactosaminyltransferase in human serum and in erythrocyte membranes. This enzyme catalyzes the transfer of N-acetyl-D-galactosamine from UDP-N-acetyl-D-galactosamine to a mucin receptor and 2'-fucosyllactose that have blood group H activity and may be responsible...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.68.8.1753

    authors： Kim YS,Perdomo J,Bella A Jr,Nordberg J

    更新日期：1971-08-01 00:00:00

  • Multifunctional supramolecular polymer networks as next-generation consolidants for archaeological wood conservation.

    abstract：:The preservation of our cultural heritage is of great importance to future generations. Despite this, significant problems have arisen with the conservation of waterlogged wooden artifacts. Three major issues facing conservators are structural instability on drying, biological degradation, and chemical degradation on ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1406037111

    authors： Walsh Z,Janeček ER,Hodgkinson JT,Sedlmair J,Koutsioubas A,Spring DR,Welch M,Hirschmugl CJ,Toprakcioglu C,Nitschke JR,Jones M,Scherman OA

    更新日期：2014-12-16 00:00:00