当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > The K+ channel KIR2.1 functions in tandem with proton influx to mediate sour taste transduction.

The K+ channel KIR2.1 functions in tandem with proton influx to mediate sour taste transduction.

Abstract：

:Sour taste is detected by a subset of taste cells on the tongue and palate epithelium that respond to acids with trains of action potentials. Entry of protons through a Zn(2+)-sensitive proton conductance that is specific to sour taste cells has been shown to be the initial event in sour taste transduction. Whether this conductance acts in concert with other channels sensitive to changes in intracellular pH, however, is not known. Here, we show that intracellular acidification generates excitatory responses in sour taste cells, which can be attributed to block of a resting K(+) current. We identify KIR2.1 as the acid-sensitive K(+) channel in sour taste cells using pharmacological and RNA expression profiling and confirm its contribution to sour taste with tissue-specific knockout of the Kcnj2 gene. Surprisingly, acid sensitivity is not conferred on sour taste cells by the specific expression of Kir2.1, but by the relatively small magnitude of the current, which makes the cells exquisitely sensitive to changes in intracellular pH. Consistent with a role of the K(+) current in amplifying the sensory response, entry of protons through the Zn(2+)-sensitive conductance produces a transient block of the KIR2.1 current. The identification in sour taste cells of an acid-sensitive K(+) channel suggests a mechanism for amplification of sour taste and may explain why weak acids that produce intracellular acidification, such as acetic acid, taste more sour than strong acids.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Ye W,Chang RB,Bushman JD,Tu YH,Mulhall EM,Wilson CE,Cooper AJ,Chick WS,Hill-Eubanks DC,Nelson MT,Kinnamon SC,Liman ER

doi

10.1073/pnas.1514282112

subject

Has Abstract

pub_date

2016-01-12 00:00:00

pages

E229-38

issue

2

eissn

0027-8424

issn

1091-6490

pii

1514282112

journal_volume

113

pub_type

杂志文章

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