Proteins with H-bond packing defects are highly interactive with lipid bilayers: Implications for amyloidogenesis.

abstract：:The envelope spike of HIV is one of the most highly N-glycosylated structures found in nature. However, despite extensive research revealing essential functional roles in infection and immune evasion, the chemical structures of the glycans on the native viral envelope glycoprotein gp120--as opposed to recombinantly ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doores KJ,Bonomelli C,Harvey DJ,Vasiljevic S,Dwek RA,Burton DR,Crispin M,Scanlan CN

更新日期：2010-08-03 00:00:00

abstract：:Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy. It is widely accepted that the oxygen sensitivity of hydrogen production, combined with competition between hydrogenases and NADPH-dependent carbon dioxide fixation are the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yacoby I,Pochekailov S,Toporik H,Ghirardi ML,King PW,Zhang S

更新日期：2011-06-07 00:00:00

abstract：:BSC-1 cells, epithelial cells of African green monkey kidney origin, show pronounced density-dependent regulation of growth in cell culture. Growth of the cells is rapid to a density of approximately 1.5 x 10(5) cells/per cm(2) in Dulbecco-modified Eagle's medium supplemented with 10% calf serum. Above this "saturatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holley RW,Armour R,Baldwin JH

更新日期：1978-01-01 00:00:00

abstract：:The reported isolation of nanobacteria from human kidney stones raises the intriguing possibility that these microorganisms are etiological agents of pathological extraskeletal calcification [Kajander, E. O. & Ciftçioglu, N. (1998) Proc. Natl. Acad. Sci. USA 95, 8274-8279]. Nanobacteria were previously isolated from F...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cisar JO,Xu DQ,Thompson J,Swaim W,Hu L,Kopecko DJ

更新日期：2000-10-10 00:00:00

abstract：:A site that is preferentially cleaved by the single-strand-specific endonuclease from Aspergillus oryzae was located in vitro 180 base pairs upstream from the 5' end of the chicken pro-alpha 2(I) collagen gene. It is found in supercoiled plasmids with a negative superhelical density of -0.024 or more but not in linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finer MH,Fodor EJ,Boedtker H,Doty P

更新日期：1984-03-01 00:00:00

abstract：:25-Hydroxycholesterol is produced in mammalian tissues. The function of this oxysterol is unknown. Here we describe a central role for 25-hydroxycholesterol in regulating the immune system. In initial experiments, we found that stimulation of macrophage Toll-like receptors (TLR) induced expression of cholesterol 25-hy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauman DR,Bitmansour AD,McDonald JG,Thompson BM,Liang G,Russell DW

更新日期：2009-09-29 00:00:00

abstract：:Environmental cues are transmitted to the interior of the cell via a complex network of signaling hubs. Receptor tyrosine kinases (RTKs) and trimeric G proteins are two such major signaling hubs in eukaryotes. Conventionally, canonical signal transduction via trimeric G proteins is thought to be triggered exclusively ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Midde KK,Aznar N,Laederich MB,Ma GS,Kunkel MT,Newton AC,Ghosh P

更新日期：2015-03-03 00:00:00

abstract：:Although x-ray crystallography is the most widely used method for macromolecular structure determination, it does not provide dynamical information, and either experimental tricks or complementary experiments must be used to overcome the inherently static nature of crystallographic structures. Here we used specific x-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colletier JP,Bourgeois D,Sanson B,Fournier D,Sussman JL,Silman I,Weik M

更新日期：2008-08-19 00:00:00

abstract：:We used a high-titer recombinant adeno-associated virus (rAAV) vector to express WT or mutant human alpha-synuclein in the substantia nigra of adult marmosets. The alpha-synuclein protein was expressed in 90-95% of all nigral dopamine neurons and distributed by anterograde transport throughout their axonal and dendrit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirik D,Annett LE,Burger C,Muzyczka N,Mandel RJ,Björklund A

更新日期：2003-03-04 00:00:00

abstract：:Interleukin 1 (IL-1) and tumor necrosis factor alpha are thought to contribute to the inflammatory response associated with autoimmune diseases. Transforming growth factor beta 1 (TGF-beta 1) counteracts many effects of these cytokines and has various immunosuppressive properties. In the present study, it is shown tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kuruvilla AP,Shah R,Hochwald GM,Liggitt HD,Palladino MA,Thorbecke GJ

更新日期：1991-04-01 00:00:00

abstract：:Cellular communities in living tissues act in concert to establish intricate microenvironments, with complexity difficult to recapitulate in vitro. We report a method for docking numerous cellularized hydrogel shapes (100-1,000 µm in size) into hydrogel templates to construct 3D cellular microenvironments. Each shape ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eng G,Lee BW,Parsa H,Chin CD,Schneider J,Linkov G,Sia SK,Vunjak-Novakovic G

更新日期：2013-03-19 00:00:00

abstract：:"Evolution behaves like a tinkerer" (François Jacob, Science, 1977). Software systems provide a singular opportunity to understand biological processes using concepts from network theory. The Debian GNU/Linux operating system allows us to explore the evolution of a complex network in a unique way. The modular design d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fortuna MA,Bonachela JA,Levin SA

更新日期：2011-12-13 00:00:00

abstract：:The DNA strand exchange protein RAD51 facilitates the central step in homologous recombination, a process fundamentally important for accurate repair of damaged chromosomes, restart of collapsed replication forks, and telomere maintenance. The active form of RAD51 is a nucleoprotein filament that assembles on single-s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Subramanyam S,Ismail M,Bhattacharya I,Spies M

更新日期：2016-10-11 00:00:00

abstract：:Malignant melanoma has one of the highest mortality rates of any cancer because of its aggressive nature and high metastatic potential. Clinical staging of the disease at the time of diagnosis is very important for the prognosis and outcome of melanoma treatment. In this study, we designed and synthesized the 18F-labe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pyo A,Kim DY,Kim H,Lim D,Kwon SY,Kang SR,Kim HS,Bom HS,Min JJ

更新日期：2020-06-09 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) signaling is thought to mediate quorum sensing in many species of Proteobacteria. The opportunistic human pathogen Pseudomonas aeruginosa uses acyl-HSLs to regulate hundreds of genes, including many that code for extracellular virulence factors. The idea that the P. aeruginosa acyl-H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yarwood JM,Volper EM,Greenberg EP

更新日期：2005-06-21 00:00:00

abstract：:Duodenal bicarbonate secretion (DBS) is accepted as the primary mucosal defense against acid discharged from the stomach and is impaired in patients with duodenal ulcer disease. The secretory response to luminal acid is the main physiological stimulus for DBS and involves mediation by PGE2 produced by mucosal cells. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leppilampi M,Parkkila S,Karttunen T,Gut MO,Gros G,Sjöblom M

更新日期：2005-10-18 00:00:00

abstract：:A rapid method for quantitating products of the oligodeoxynucleotide processing reaction in vitro has been developed to facilitate enzymatic studies of the retroviral integrases. Unlike earlier procedures, this assay does not depend on polyacrylamide gel electrphoresis but separates products by batch adsorption to PEI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller B,Jones KS,Merkel GW,Skalka AM

更新日期：1993-12-15 00:00:00

abstract：:Nitric oxide-releasing compounds were shown to activate an ADP-ribosyltransferase activity in the cytosol of Dictyostelium discoideum. The enzyme ADP-ribosylated a cytosolic protein of approximately 41 kDa, p41. Neither cGMP nor GTP and its analogues affected this ADP-ribosylation. p41 differs from other substrates AD...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao Y,Howlett A,Klein C

更新日期：1992-07-01 00:00:00

abstract：:Valproic acid (VPA) is an anticonvulsant drug that is also used to treat migraines and bipolar disorder. Its proposed biological targets include human voltage-gated sodium channels, among other membrane proteins. We used the prokaryotic NavMs sodium channel, which has been shown to be a good exemplar for drug binding ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zanatta G,Sula A,Miles AJ,Ng LCT,Torella R,Pryde DC,DeCaen PG,Wallace BA

更新日期：2019-12-10 00:00:00

abstract：:A model of the photochemical apparatus of photosynthesis, presented previously in a tripartite format, is used in a bipartite format to analyze energy transfer between photosystem II units. The model is used to develop analytical expressions for the photochemical properties of chloroplasts that include a term for the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler WL

更新日期：1980-08-01 00:00:00

abstract：:Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open rea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nies DH,Nies A,Chu L,Silver S

更新日期：1989-10-01 00:00:00

abstract：:Microstructural, δ(13)C isotope and C/N ratio investigations were conducted on excavated material from the black Younger Dryas boundary in Lommel, Belgium, aiming for a characterisation of the carbon content and structures. Cubic diamond nanoparticles are found in large numbers. The larger ones with diameters around o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tian H,Schryvers D,Claeys P

更新日期：2011-01-04 00:00:00

abstract：:Membrane-associated and periplasmic proteins of Agrobacterium tumefaciens C-58 were compared with those from avirulent (nontumrigenic) derivative strains by slab and two-dimensional gel electrophoresis. Two proteins (Per-I and Per-2), with a molecular weight of 37,500 and 37,300, respectively, were detected in the sup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sonoki S,Kado CI

更新日期：1978-08-01 00:00:00

abstract：:The role of gene interactions in the evolutionary process has long been controversial. Although some argue that they are not of importance, because most variation is additive, others claim that their effect in the long term can be substantial. Here, we focus on the long-term effects of genetic interactions under direc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paixão T,Barton NH

更新日期：2016-04-19 00:00:00

abstract：:Influenza virus neuraminidase (NA; EC 3.2.1.18) possesses a signal-anchor hydrophobic domain at the amino terminus. To characterize the nature of this signal-anchor domain we have introduced single amino acid changes in this domain by oligonucleotide-directed mutagenesis. Three mutant NA proteins that were synthesized...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sivasubramanian N,Nayak DP

更新日期：1987-01-01 00:00:00

abstract：:Reconstructing the origin and early evolutionary history of anthropoid primates (monkeys, apes, and humans) is a current focus of paleoprimatology. Although earlier hypotheses frequently supported an African origin for anthropoids, recent discoveries of older and phylogenetically more basal fossils in China and Myanma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaimanee Y,Chavasseau O,Beard KC,Kyaw AA,Soe AN,Sein C,Lazzari V,Marivaux L,Marandat B,Swe M,Rugbumrung M,Lwin T,Valentin X,Zin-Maung-Maung-Thein,Jaeger JJ

更新日期：2012-06-26 00:00:00

abstract：:Thrombin induces platelet aggregation and formation of a fibrin clot in platelet-rich plasma; leupeptin, a protease inhibitor, partially inhibits platelet aggregation, but it does not inhibit fibrin clot formation. Indomethacin does not inhibit either thrombin-induced platelet aggregation or fibrin clot formation. How...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruggiero M,Lapetina EG

更新日期：1986-05-01 00:00:00

abstract：:Some realistic galaxy models have been found to support discrete unstable spiral modes. Here, through the study of the relevant physical mechanisms and an extensive numerical investigation of the properties of the dominant modes in a wide class of galactic equilibria, we show how spiral structures are excited with dif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haass J,Bertin G,Lin CC

更新日期：1982-06-01 00:00:00

abstract：:Tetrodotoxin-sensitive sodium channels are detectable in normal human fibroblasts and in "glia-like" cells at appreciable levels when compared to what is observed in established neuronal cell lines in culture. Two- to 3-fold stimulations of sodium influx are observed in the presence of 0.2 mM veratridine and scorpion ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Munson R Jr,Westermark B,Glaser L

更新日期：1979-12-01 00:00:00

abstract：:Loss of IκB kinase (IKK) β-dependent NF-κB signaling in hematopoietic cells is associated with increased granulopoiesis. Here we identify a regulatory cytokine loop that causes neutrophilia in Ikkβ-deficient mice. TNF-α-dependent apoptosis of myeloid progenitor cells leads to the release of IL-1β, which promotes Th17 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mankan AK,Canli O,Schwitalla S,Ziegler P,Tschopp J,Korn T,Greten FR

更新日期：2011-04-19 00:00:00