Multimodular biosensors reveal a novel platform for activation of G proteins by growth factor receptors.

abstract：:The knowledge of tropical palaeoclimates is crucial for understanding global climate change, because it is a test bench for general circulation models that are ultimately used to predict future global warming. A longstanding issue concerning the last glacial maximum in the tropics is the discrepancy between the decrea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu H,Guiot J,Brewer S,Guo Z,Peng C

更新日期：2007-06-05 00:00:00

abstract：:Population growth rate is a fundamental ecological and evolutionary characteristic of living organisms, but individuals must balance the metabolism devoted to biosynthesis and reproduction against the maintenance of existing structure and other functionality. Here we present a mathematical model that relates metabolic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempes CP,Dutkiewicz S,Follows MJ

更新日期：2012-01-10 00:00:00

abstract：:Chromopeptides were prepared by proteolytic digestion of phytochrome (far-red absorbing form, P(fr)) and of phycocyanin. The phycocyanobilin peptide, the chromophore of which is Z,Z,Z-configurated, was modified to the Z,Z,E isomeric chromophore. It has been demonstrated earlier that the P(fr) chromopeptide and the Z,Z...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rüdiger W,Thümmler F,Cmiel E,Schneider S

更新日期：1983-10-01 00:00:00

abstract：:The DNA strand exchange protein RAD51 facilitates the central step in homologous recombination, a process fundamentally important for accurate repair of damaged chromosomes, restart of collapsed replication forks, and telomere maintenance. The active form of RAD51 is a nucleoprotein filament that assembles on single-s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Subramanyam S,Ismail M,Bhattacharya I,Spies M

更新日期：2016-10-11 00:00:00

abstract：:The initial electron transfer steps in the photosynthetic reaction center of the purple bacterium Rhodobacter sphaeroides have been investigated by femtosecond time-resolved spectroscopy. The experimental data taken at various wavelengths demonstrate the existence of at least four intermediate states within the first ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holzapfel W,Finkele U,Kaiser W,Oesterhelt D,Scheer H,Stilz HU,Zinth W

更新日期：1990-07-01 00:00:00

abstract：:The blood-aqueous barrier plays a key role in regulating aqueous humor homeostasis by selectively restricting passage of proteins into the eye. The kinetics of aqueous flow are traditionally measured using artificial markers; however, these marker molecules do not address the barrier's selective permeability to plasma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu P,Thomson BR,Khalatyan N,Feng L,Liu X,Savas JN,Quaggin SE,Jin J

更新日期：2018-09-04 00:00:00

abstract：:To investigate the properties of metazoan replication origins, recent studies in cell culture have adopted the strategy of identifying origins using genome-wide approaches and assessing correlations with such features as transcription and histone modifications. Drosophila amplicon in follicle cells (DAFCs), genomic re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim JC,Orr-Weaver TL

更新日期：2011-10-04 00:00:00

abstract：:The metal-binding motif in the sequence of leukotriene A4 (LTA4) (EC 3.3.2.6), a bifunctional zinc metalloenzyme, contains a glutamic acid that is conserved in several zinc hydrolases. To study its role for the two catalytic activities, Glu-296 in mouse leukotriene A4 hydrolase was replaced by a glutamine or alanine r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wetterholm A,Medina JF,Rådmark O,Shapiro R,Haeggström JZ,Vallee BL,Samuelsson B

更新日期：1992-10-01 00:00:00

abstract：:Cell membrane contact induces the de novo expression of choline O-acetyltransferase (CAT; acetyl-CoA: choline O-acetyltransferase, EC 2.3.1.6) activity in cultures of virtually pure neonatal rat dissociated sympathetic neurons. To identify molecular mechanisms underlying membrane-associated CAT induction, the responsi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adler JE,Schleifer LS,Black IB

更新日期：1989-02-01 00:00:00

abstract：:The epithelial-derived cytokine thymic stromal lymphopoietin (TSLP) is important for the initiation of allergic airway inflammation through a dendritic cell-mediated T helper 2 response. To identify the factors that control TSLP expression, we examined the ability of inflammatory mediators to regulate TSLP production ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee HC,Ziegler SF

更新日期：2007-01-16 00:00:00

abstract：:The mechanism underlying how transcription factors regulate mesenchymal stem cell lineage commitment remains unclear. To determine the role of core-binding factor subunit beta (Cbfβ) in osteoblast lineage commitment, we generated three mouse models by deleting Cbfβ at different osteoblast lineage stages. We demonstrat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu M,Wang Y,Shao JZ,Wang J,Chen W,Li YP

更新日期：2017-09-19 00:00:00

abstract：:The tremendous pandemic potential of coronaviruses was demonstrated twice in the past few decades by two global outbreaks of deadly pneumonia. The coronavirus spike (S) glycoprotein initiates infection by promoting fusion of the viral and cellular membranes through conformational changes that remain largely uncharacte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walls AC,Tortorici MA,Snijder J,Xiong X,Bosch BJ,Rey FA,Veesler D

更新日期：2017-10-17 00:00:00

abstract：:Lines containing two third-chromosome inversions (Arrowhead and Pikes Peak) of Drosophila pseudoobscura selected for fast and slow mating speed were crossed to a multichromosomal, dominant marker inversion stock, and strains with various combinations of chromosomal derivation were tested for their mating activity. Whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sherwin RN,Spiess EB

更新日期：1973-02-01 00:00:00

abstract：:Size has a profound effect on the structure of the brain. Many brain structures scale allometrically, that is, their relative size changes systematically as a function of brain size. Here we use independent contrasts analysis to examine the scaling of frontal cortex in 43 species of mammals including 25 primates and 1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bush EC,Allman JM

更新日期：2004-03-16 00:00:00

abstract：:Huntington disease (HD) is a neurodegenerative disorder caused by a CAG expansion within the huntingtin gene that encodes a polymorphic glutamine tract at the amino terminus of the huntingtin protein. HD is one of nine polyglutamine expansion diseases. The clinical threshold of polyglutamine expansion for HD is near 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caron NS,Desmond CR,Xia J,Truant R

更新日期：2013-09-03 00:00:00

abstract：:The synthesis of graphene materials is typically carried out by oxidizing graphite to graphite oxide followed by a reduction process. Numerous methods exist for both the oxidation and reduction steps, which causes unpredictable contamination from metallic impurities into the final material. These impurities are known ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong CH,Sofer Z,Kubešová M,Kučera J,Matějková S,Pumera M

更新日期：2014-09-23 00:00:00

abstract：:Monoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs. Here we present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishchenko A,Wacker D,Kapoor M,Zhang A,Han GW,Basu S,Patel N,Messerschmidt M,Weierstall U,Liu W,Katritch V,Roth BL,Stevens RC,Cherezov V

更新日期：2017-08-01 00:00:00

abstract：:Brefeldin A, a fungal metabolite that inhibits membrane transport, induces the mono(ADP-ribosyl)ation of two cytosolic proteins of 38 and 50 kDa as judged by SDS/PAGE. The 38-kDa substrate has been previously identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report that the 50-kDa BFA-induced ADP-ribo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Girolamo M,Silletta MG,De Matteis MA,Braca A,Colanzi A,Pawlak D,Rasenick MM,Luini A,Corda D

更新日期：1995-07-18 00:00:00

abstract：:Photosynthesis relies on the delicate interplay between a specific set of membrane-bound pigment-protein complexes that harvest and transport solar energy, execute charge separation, and conserve the energy. We have investigated the organization of the light-harvesting (LH) and reaction-center (RC) complexes in native...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frese RN,Siebert CA,Niederman RA,Hunter CN,Otto C,van Grondelle R

更新日期：2004-12-28 00:00:00

abstract：:Molecular oxygen (O(2)) began to accumulate in the atmosphere and surface ocean ca. 2,400 million years ago (Ma), but the persistent oxygenation of water masses throughout the oceans developed much later, perhaps beginning as recently as 580-550 Ma. For much of the intervening interval, moderately oxic surface waters ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnston DT,Wolfe-Simon F,Pearson A,Knoll AH

更新日期：2009-10-06 00:00:00

abstract：:Realizing the goal of the Paris Agreement to limit global warming to 2 °C by the end of this century will most likely require deployment of carbon-negative technologies. It is particularly important that China, as the world's top carbon emitter, avoids being locked into carbon-intensive, coal-fired power-generation te...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu X,Cao L,Wang H,Peng W,Xing J,Wang S,Cai S,Shen B,Yang Q,Nielsen CP,McElroy MB

更新日期：2019-04-23 00:00:00

abstract：:Tropospheric O(3) and sulfate both contribute to air pollution and climate forcing. There is a growing realization that air quality and climate change issues are strongly connected. To date, the importance of the coupling between O(3) and sulfate has not been fully appreciated, and thus regulations treat each pollutan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Unger N,Shindell DT,Koch DM,Streets DG

更新日期：2006-03-21 00:00:00

abstract：:Although cells express hundreds of metalloenzymes, the mechanisms by which apoenzymes receive their metal cofactors are largely unknown. Poly(rC)-binding proteins PCBP1 and PCBP2 are multifunctional adaptor proteins that bind iron and deliver it to ferritin for storage or to prolyl and asparagyl hydroxylases to metall...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frey AG,Nandal A,Park JH,Smith PM,Yabe T,Ryu MS,Ghosh MC,Lee J,Rouault TA,Park MH,Philpott CC

更新日期：2014-06-03 00:00:00

abstract：:The availability of gene-targeted mice deficient in the urokinase-type plasminogen activator (uPA), urokinase receptor (uPAR), tissue-type plasminogen activator (tPA), and plasminogen permits a critical, genetic-based analysis of the physiological and pathological roles of the two mammalian plasminogen activators. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bugge TH,Flick MJ,Danton MJ,Daugherty CC,Romer J,Dano K,Carmeliet P,Collen D,Degen JL

更新日期：1996-06-11 00:00:00

abstract：:It has long been known that cholera outbreaks can be initiated when Vibrio cholerae, the bacterium that causes cholera, is present in drinking water in sufficient numbers to constitute an infective dose, if ingested by humans. Outbreaks associated with drinking or bathing in unpurified river or brackish water may dire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lobitz B,Beck L,Huq A,Wood B,Fuchs G,Faruque AS,Colwell R

更新日期：2000-02-15 00:00:00

abstract：:Mutations of esterase 3 confer two forms of organophosphate resistance on contemporary Australasian Lucilia cuprina. One form, called diazinon resistance, is slightly more effective against commonly used insecticides and is now more prevalent than the other form, called malathion resistance. We report here that the si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hartley CJ,Newcomb RD,Russell RJ,Yong CG,Stevens JR,Yeates DK,La Salle J,Oakeshott JG

更新日期：2006-06-06 00:00:00

abstract：:Lysine to methionine (K-to-M) mutations in genes encoding histone H3 are thought to drive a subset of pediatric brain and bone cancers. These high-frequency K-to-M mutations occur at sites of methylation on histone H3, and tumors containing the mutant histones exhibit a global loss of specific histone methylation mark...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jayaram H,Hoelper D,Jain SU,Cantone N,Lundgren SM,Poy F,Allis CD,Cummings R,Bellon S,Lewis PW

更新日期：2016-05-31 00:00:00

abstract：:The structures of the rearranged c-myc gene products derived from a rcp T(12;15) have been investigated in the MPC-11 (IgG2b kappa) plasmacytoma. The rcp T(12;15) in MPC-11 is a reciprocal exchange between the c-myc gene on chromosome 15 and the immunoglobulin gamma 2a switch region (S gamma 2a) on chromosome 12. The ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stanton LW,Yang JQ,Eckhardt LA,Harris LJ,Birshtein BK,Marcu KB

更新日期：1984-02-01 00:00:00

abstract：:Previous studies have shown that Arthrobacter pyridinolis can transport D-fructose or L-rhamnose using either a phosphoenolpyruvate:hexose phosphotransferase (phosphoenolpyruvate:protein phosphotransferase, EC 2.7.3.9) system or a respiration-coupled transport system which requires the presence of exogenous L-malate. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolfson EB,Krulwich TA

更新日期：1974-05-01 00:00:00

abstract：:The Mars Organic Analyzer (MOA), a microfabricated capillary electrophoresis (CE) instrument for sensitive amino acid biomarker analysis, has been developed and evaluated. The microdevice consists of a four-wafer sandwich combining glass CE separation channels, microfabricated pneumatic membrane valves and pumps, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skelley AM,Scherer JR,Aubrey AD,Grover WH,Ivester RH,Ehrenfreund P,Grunthaner FJ,Bada JL,Mathies RA

更新日期：2005-01-25 00:00:00