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Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by low molecular weight nutrients.

Abstract：

:BSC-1 cells, epithelial cells of African green monkey kidney origin, show pronounced density-dependent regulation of growth in cell culture. Growth of the cells is rapid to a density of approximately 1.5 x 10(5) cells/per cm(2) in Dulbecco-modified Eagle's medium supplemented with 10% calf serum. Above this "saturation density," growth is much slower. It has been found that the glucose concentration in the culture medium is important in determining the "saturation density." If the glucose concentration is increased 4-fold, the "saturation density" increases approximately 50%. Reduction of the "saturation density" of BSC-1 cells is also possible by decreasing the concentrations of low molecular weight nutrients in the culture medium. In medium supplemented with 0.1% calf serum, decreasing the concentrations of all of the organic constituents of the medium, from the high levels present in Dulbecco-modified Eagle's medium to concentrations near physiological levels, decreases the "saturation density" by approximately half. The decreased "saturation density" is not the result of lowering the concentration of any single nutrient but rather results from reduction of the concentrations of several nutrients. When the growth of BSC-1 cells is limited by low concentrations of all of the nutrients, some stimulation of growth results from increasing, separately, the concentrations of individual groups of nutrients, but the best growth stimulation is obtained by increasing the concentrations of all of the nutrients. The "wound healing" phenomenon, one manifestation of density-dependent regulation of growth in cell culture, is abolished by lowering the concentration of glutamine in the medium. Density-dependent regulation of growth of BSC-1 cells in cell culture thus appears to be a complex phenomenon that involves an interaction of nutrient concentrations with other regulatory factors.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Holley RW,Armour R,Baldwin JH

doi

10.1073/pnas.75.1.339

subject

Has Abstract

pub_date

1978-01-01 00:00:00

pages

339-41

issue

eissn

0027-8424

issn

1091-6490

journal_volume

pub_type

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Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by low molecular weight nutrients.