A common allosteric site and mechanism in caspases.

abstract：:Lampreys are one of the two surviving jawless vertebrate groups and one of a few vertebrate groups with the best exemplified metamorphosis during their life cycle, which consists of a long-lasting larval stage, a peculiar metamorphosis, and a relatively short adulthood with a markedly different anatomy. Although the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang MM,Wu F,Miao D,Zhang J

更新日期：2014-10-28 00:00:00

abstract：:Three protein kinases (ATP:protein phosphotransferase, EC 2.7.1.37) were detected when the soluble fraction of rabbit kidney medulla was chromatographed on DEAE-cellulose with a linear NaC1 gradient. The first two kinases eluted (Peak 1 and Peak II) were cyclic-AMP-dependent, wheras Peak III was cyclic-AMP-independent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlender KK,Reimann EM

更新日期：1975-06-01 00:00:00

abstract：:Research has previously shown that, without methionine supplements, neural tube proteins of rat embryos cultured on bovine sera were hypomethylated and neural tubes failed to close. In the present study, to identify the proteins that became methylated during neurulation, rat embryos were first cultured on methionine-d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moephuli SR,Klein NW,Baldwin MT,Krider HM

更新日期：1997-01-21 00:00:00

abstract：:Pronounced bright and dark bands are seen at the bright and dark edges of half-shadows and similar distributions of illumination. These are the so-called Mach bands. A pair of vertical Mach bands was generated with a ramp pattern in the central strip of a horizontal tripartite oscilloscope display. This pattern consis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratliff F,Milkman N,Rennert N

更新日期：1983-07-01 00:00:00

abstract：:Markov chain Monte Carlo (MCMC) or the Metropolis-Hastings algorithm is a simulation algorithm that has made modern Bayesian statistical inference possible. Nevertheless, the efficiency of different Metropolis-Hastings proposal kernels has rarely been studied except for the Gaussian proposal. Here we propose a unique ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Z,Rodríguez CE

更新日期：2013-11-26 00:00:00

abstract：:A cDNA of the human terminal deoxynucleotidyltransferase (TdT; "terminal transferase," EC 2.7.7.31) was isolated from a human lymphoblastoid cell cDNA library in lambda gt 11 by using immunological procedures. Four inserts containing 723 to 939 base pairs were recloned in pBR322 for hybridization and preliminary seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peterson RC,Cheung LC,Mattaliano RJ,Chang LM,Bollum FJ

更新日期：1984-07-01 00:00:00

abstract：:Deregulation of microRNA (miRNA) expression can lead to cancer initiation and progression. However, limited information exists on the function of miRNAs in cancer maintenance. We examined these issues in the case of myeloproliferative diseases and neoplasms (MPN), a collection of hematopoietic neoplasms regarded as pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo S,Bai H,Megyola CM,Halene S,Krause DS,Scadden DT,Lu J

更新日期：2012-10-09 00:00:00

abstract：:In the nitrogen-fixing symbiosis between legume hosts and rhizobia, the bacteria are engulfed by a plant cell membrane to become intracellular organelles. In the model legume Medicago truncatula, internalization and differentiation of Sinorhizobium (also known as Ensifer) meliloti is a prerequisite for nitrogen fixati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim M,Chen Y,Xi J,Waters C,Chen R,Wang D

更新日期：2015-12-08 00:00:00

abstract：:We have isolated three classes of monoclonal antibodies against Drosophila cell-surface antigens that are expressed with positional specificity in imaginal discs. Comparison of immunofluorescence patterns with the wing-disc fate map reveals that expression of the antigens is not directly related to the specific type o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brower DL,Wilcox M,Piovant M,Smith RJ,Reger LA

更新日期：1984-12-01 00:00:00

abstract：:The thesis is developed that a low oxygen level Precambrian atmosphere presented early-evolving metazoan organisms with physiological connective tissue priorities resulting from the important molecular oxygen requirements in the biosynthesis of collagen hydroxyproline. Shells, cuticles, and carapaces which are not man...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Towe KM

更新日期：1970-04-01 00:00:00

abstract：:P450c17 is the single enzyme mediating both 17 alpha-hydroxylase (steroid 17 alpha-monooxygenase, EC 1.14.99.9) and 17,20 lyase activities in the synthesis of steroid hormones. It has been suggested that different P450c17 isozymes mediate these activities in the adrenal gland and testis. We sequenced 423 of the 509 am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung BC,Picado-Leonard J,Haniu M,Bienkowski M,Hall PF,Shively JE,Miller WL

更新日期：1987-01-01 00:00:00

abstract：:The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of antibody 28B4 complexed with hapten have been solved at 2.2-angstrom and 1.9-angstrom resolution, respectively. To our knowledge, these structures are the highest resolution catalytic antibody structures to date and provide insight into the molec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsieh-Wilson LC,Schultz PG,Stevens RC

更新日期：1996-05-28 00:00:00

abstract：:Oxidative stress exacerbates neovascularization (NV) in many disease processes. In this study we investigated the mechanism of that effect. Mice deficient in superoxide dismutase 1 (Sod1(-/-) mice) have increased oxidative stress and show severe ocular NV that is reduced to baseline by antioxidants. Compared with wild...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong A,Shen J,Zeng M,Campochiaro PA

更新日期：2011-08-30 00:00:00

abstract：:Stepwise, synthetic alteration of oxytocin, guided by conformational and enzymological considerations, has led to analogs with specifically modified activity profiles and resistance to enzymatic inactivation. General requirements for the design of peptides with therapeutic value are discussed. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter R,Yamanaka T,Sakakibara S

更新日期：1974-05-01 00:00:00

abstract：:The eukaryotic DNA sliding clamp that keeps DNA polymerase engaged at a replication fork, called proliferating cell nuclear antigen (PCNA), is loaded onto the 3' ends of primer DNA through its interaction with a heteropentameric protein complex called replication factor C (RFC). The ATPase activity of RFC is necessary...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shiomi Y,Usukura J,Masamura Y,Takeyasu K,Nakayama Y,Obuse C,Yoshikawa H,Tsurimoto T

更新日期：2000-12-19 00:00:00

abstract：:Epithelial growth factor-like 7 (EGFL7) is a protein that is secreted by endothelial cells and plays an important role in angiogenesis. Although EGFL7 is aberrantly overexpressed in solid tumors, its role in leukemia has not been evaluated. Here, we report that levels of both EGFL7 mRNA and EGFL7 protein are increased...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papaioannou D,Shen C,Nicolet D,McNeil B,Bill M,Karunasiri M,Burke MH,Ozer HG,Yilmaz SA,Zitzer N,Behbehani GK,Oakes CC,Steiner DJ,Marcucci G,Powell BL,Kolitz JE,Carter TH,Wang ES,Mrózek K,Croce CM,Caligiuri MA,Bl

更新日期：2017-06-06 00:00:00

abstract：:We analyze Niels Bohr's proposed two-slit interference experiment with highly charged particles which argues that the consistency of elementary quantum mechanics requires that the electromagnetic field must be quantized. In the experiment a particle's path through the slits is determined by measuring the Coulomb field...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baym G,Ozawa T

更新日期：2009-03-03 00:00:00

abstract：:The overall structure of the transition-state and intermediate ensembles observed experimentally for dihydrofolate reductase and interleukin-1beta can be obtained by using simplified models that have almost no energetic frustration. The predictive power of these models suggests that, even for these very large proteins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clementi C,Jennings PA,Onuchic JN

更新日期：2000-05-23 00:00:00

abstract：:Preadipocytes can give rise to either white adipocytes or beige adipocytes. Owing to their distinct abilities in nutrient storage and energy expenditure, strategies that specifically promote "beiging" of adipocytes hold great promise for counterbalancing obesity and metabolic diseases. Yet, factors dictating the diffe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu K,Lin L,Li Q,Xue Y,Zheng F,Wang G,Zheng C,Du L,Hu M,Huang Y,Shao C,Kong X,Melino G,Shi Y,Wang Y

更新日期：2020-02-04 00:00:00

abstract：:Malaria control is threatened by a limited pipeline of effective pharmaceuticals against drug-resistant strains of Plasmodium falciparum Components of the mitochondrial electron transport chain (ETC) are attractive targets for drug development, owing to exploitable differences between the parasite and human ETC. Disru...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lane KD,Mu J,Lu J,Windle ST,Liu A,Sun PD,Wellems TE

更新日期：2018-06-12 00:00:00

abstract：:Bright, photostable, and nontoxic fluorescent contrast agents are critical for biological imaging. "Self-healing" dyes, in which triplet states are intramolecularly quenched, enable fluorescence imaging by increasing fluorophore brightness and longevity, while simultaneously reducing the generation of reactive oxygen ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pati AK,El Bakouri O,Jockusch S,Zhou Z,Altman RB,Fitzgerald GA,Asher WB,Terry DS,Borgia A,Holsey MD,Batchelder JE,Abeywickrama C,Huddle B,Rufa D,Javitch JA,Ottosson H,Blanchard SC

更新日期：2020-09-29 00:00:00

abstract：:Here we report the development of an in vivo system to study the interaction of stem cells with drugs using a tumor model in the adult Drosophila intestine. Strikingly, we find that some Food and Drug Administration-approved chemotherapeutics that can inhibit the growth of Drosophila tumor stem cells can paradoxically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markstein M,Dettorre S,Cho J,Neumüller RA,Craig-Müller S,Perrimon N

更新日期：2014-03-25 00:00:00

abstract：:The role of chemotaxis in the virulence of gastrointestinal pathogens is ill defined. Counterintuitively, nonchemotactic mutants of the polarly flagellated pathogen Vibrio cholerae greatly out-compete the wild-type strain during infection of the small intestine. We show that the out-competition phenotype is dependent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler SM,Camilli A

更新日期：2004-04-06 00:00:00

abstract：:Metabolic engineering seeks to reprogram microbial cells to efficiently and sustainably produce value-added compounds. Since chemical production can be at odds with the cell's natural objectives, strategies have been developed to balance conflicting goals. For example, dynamic regulation modulates gene expression to f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dinh CV,Prather KLJ

更新日期：2019-12-17 00:00:00

abstract：:Bacteriophage Mu was inserted into the unc genes of Escherichia coli. The resulting mutation AS12 had a polar effect on the unc operon: membranes of the mutant AS12 contained the dicyclohexylcarbodiimide-binding protein c and the protein a as sole subunits of the ATP synthase. It was shown by peptide mapping and amino...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedl P,Bienhaus G,Hoppe J,Schairer HU

更新日期：1981-11-01 00:00:00

abstract：:Perilipin coats the lipid droplets of adipocytes and is thought to have a role in regulating triacylglycerol hydrolysis. To study the role of perilipin in vivo, we have created a perilipin knockout mouse. Perilipin null (peri(-/-)) and wild-type (peri(+/+)) mice consume equal amounts of food, but the adipose tissue ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tansey JT,Sztalryd C,Gruia-Gray J,Roush DL,Zee JV,Gavrilova O,Reitman ML,Deng CX,Li C,Kimmel AR,Londos C

更新日期：2001-05-22 00:00:00

abstract：:Although neurotrophins are primarily associated with long-term effects on neuronal survival and differentiation, recent studies have shown that acute changes in synaptic transmission can also be produced. In the hippocampus, an area critically involved in learning and memory, we have found that brain-derived neurotrop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine ES,Dreyfus CF,Black IB,Plummer MR

更新日期：1995-08-15 00:00:00

abstract：:Aging and disease processes may be due to deleterious and irreversible changes produced by free radical reactions. The enzyme copper-zinc superoxide dismutase (Cu-Zn SOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) performs a protective function by scavenging superoxide radicals. The Cu-Zn SOD gene (Sod) cloned...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seto NO,Hayashi S,Tener GM

更新日期：1990-06-01 00:00:00

abstract：:Documented cases of convergent molecular evolution due to selection are fairly unusual, and examples to date have involved only a few amino acid positions. However, because convergence mimics shared ancestry and is not accommodated by current phylogenetic methods, it can strongly mislead phylogenetic inference when it...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castoe TA,de Koning AP,Kim HM,Gu W,Noonan BP,Naylor G,Jiang ZJ,Parkinson CL,Pollock DD

更新日期：2009-06-02 00:00:00

abstract：:This paper reports a general in situ method to grow a polymer conjugate solely from the C terminus of a recombinant protein. GFP was fused at its C terminus with an intein; cleavage of the intein provided a unique thioester moiety at the C terminus of GFP that was used to install an atom transfer radical polymerizatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao W,Liu W,Christensen T,Zalutsky MR,Chilkoti A

更新日期：2010-09-21 00:00:00