Abstract:
:Research has previously shown that, without methionine supplements, neural tube proteins of rat embryos cultured on bovine sera were hypomethylated and neural tubes failed to close. In the present study, to identify the proteins that became methylated during neurulation, rat embryos were first cultured on methionine-deficient bovine serum for 40 hr, then incubated with puromycin for 1 hr, and, finally, incubated with [methyl-14C]methionine and puromycin for 5 hr. On the basis of molecular weights, isoelectric points, and Western immunoblots, the methyl-14C-labeled proteins were identified as actin, alpha beta-tubulin, and neurofilament L. Indirect immunofluorescence studies indicated that without the addition of methionine to the culture, localization of actin and alpha beta-tubulin in the basal cytoplasm did not occur and these neuroepithelial cells lost their columnar morphology.
journal_name
Proc Natl Acad Sci U S Aauthors
Moephuli SR,Klein NW,Baldwin MT,Krider HMdoi
10.1073/pnas.94.2.543subject
Has Abstractpub_date
1997-01-21 00:00:00pages
543-8issue
2eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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