Abstract:
:Replication of the single-stranded DNA genome of geminiviruses occurs via a double-stranded intermediate that is subsequently used as a template for rolling-circle replication of the viral strand. Only one of the proteins encoded by the virus, here referred to as replication initiator protein (Rep protein), is indispensable for replication. We show that the Rep protein of tomato yellow leaf curl virus initiates viral-strand DNA synthesis by introducing a nick in the plus strand within the nonanucleotide 1TAATATT decreases 8AC, identical among all geminiviruses. After cleavage, the Rep protein remains bound to the 5' end of the cleaved strand. In addition, we show that the Rep protein has a joining activity, suggesting that it acts as a terminase, thus resolving the nascent viral single strand into genome-sized units.
journal_name
Proc Natl Acad Sci U S Aauthors
Laufs J,Traut W,Heyraud F,Matzeit V,Rogers SG,Schell J,Gronenborn Bdoi
10.1073/pnas.92.9.3879subject
Has Abstractpub_date
1995-04-25 00:00:00pages
3879-83issue
9eissn
0027-8424issn
1091-6490journal_volume
92pub_type
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