Structure and developmental expression of a sea urchin fibrillar collagen gene.

abstract：:The human epidermal growth factor receptor 2 (HER2) is specifically overexpressed in tumors of several cancers, including an aggressive form of breast cancer. It is therefore a target for both cancer diagnostics and therapy. The 58 amino acid residue Zher2 affibody molecule was previously engineered as a high-affinity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eigenbrot C,Ultsch M,Dubnovitsky A,Abrahmsén L,Härd T

更新日期：2010-08-24 00:00:00

abstract：:Ecological "surprises" are defined as unexpected findings about the natural environment. They are critically important in ecology because they are catalysts for questioning and reformulating views of the natural world, help shape assessments of the veracity of a priori predictions about ecological trends and phenomena...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindenmayer DB,Likens GE,Krebs CJ,Hobbs RJ

更新日期：2010-12-21 00:00:00

abstract：:During nervous system development, synapses undergo morphological change as a function of electrical activity. In Drosophila, enhanced activity results in the expansion of larval neuromuscular junctions. We have examined whether these structural changes involve the pre- or postsynaptic partner by selectively enhancing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mosca TJ,Carrillo RA,White BH,Keshishian H

更新日期：2005-03-01 00:00:00

abstract：:Sensory information from the external world is inherently ambiguous, necessitating prior experience as a constraint on perception. Prolonged experience (adaptation) induces perception of ambiguous morph faces as a category different from the adapted category, suggesting sensitivity in underlying neural codes to differ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furl N,van Rijsbergen NJ,Treves A,Friston KJ,Dolan RJ

更新日期：2007-08-14 00:00:00

abstract：:Residents in the marine intertidal, the zone where terrestrial and marine habitats converge, inhabit an environment that is subject to both the 24-h day and night daily rhythm of the terrestrial earth and also the 12.4-h ebb and flow of the tidal cycle. Here, we investigate the relative contribution of the daily and t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Connor KM,Gracey AY

更新日期：2011-09-20 00:00:00

abstract：:Aggregation chimeras were formed between C57BL/6 mice heterozygous for the Apc(min) (Min) mutation and wild-type SWR mice, that differ in their Pla2g2a status, a modifier of Apc(min), and also in their resistance to intestinal polyp formation. Variation in the dolichos biflorus agglutinin-staining patterns of the inte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Novelli MR,Wasan H,Rosewell I,Bee J,Tomlinson IP,Wright NA,Bodmer WF

更新日期：1999-10-26 00:00:00

abstract：:The ATP-dependent enzyme dethiobiotin synthetase from Escherichia coli catalyses the formation of dethiobiotin from CO2 and 7, 8-diaminopelargonic acid. The reaction is initiated by the formation of a carbamate and proceeds through a phosphorylated intermediate, a mixed carbamic phosphoric anhydride. Here, we report t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Käck H,Gibson KJ,Lindqvist Y,Schneider G

更新日期：1998-05-12 00:00:00

abstract：:A method is presented for detection of lysosomal hydrolase activity in situ in colonies of Chinese hamster ovary cells. This method was used to screen for mutants deficient in lysosomal alpha-mannosidase. Mutagenized cells were replicated onto filter papers [Esko, J. D. & Raetz, C. R. H. (1978) Proc. Natl. Acad. Sci. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robbins AR

更新日期：1979-04-01 00:00:00

abstract：:Pancreatic islets regulate glucose homeostasis through coordinated actions of hormone-secreting cells. What underlies the function of the islet as a unit is the close approximation and communication among heterogeneous cell populations, but the structural mediators of islet cellular cross talk remain incompletely char...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes JW,Cho JH,Conway HE,DiGruccio MR,Ng XW,Roseman HF,Abreu D,Urano F,Piston DW

更新日期：2020-04-21 00:00:00

abstract：:Proteins of plant cell walls serve as structural macromolecules and play important roles in morphogenesis and development but have not been reported to be the origins of peptide signals that activate genes for plant defense. We report here that the mRNA coding the tomato leaf polyprotein precursor of three hydroxyprol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narváez-Vásquez J,Pearce G,Ryan CA

更新日期：2005-09-06 00:00:00

abstract：:How individuals make decisions has been a matter of long-standing debate among economists and researchers in the life sciences. In economics, subjects are viewed as optimal decision makers who maximize their overall reward income. This framework has been widely influential, but requires a complete knowledge of the rew...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubanek J

更新日期：2017-08-08 00:00:00

abstract：:Metazoan embryogenesis is controlled by a limited number of signaling modules that are used repetitively at successive developmental stages. The development of social amoebas shows similar reiterated use of cAMP-mediated signaling. In the model Dictyostelium discoideum, secreted cAMP acting on 4 cAMP receptors (cARs1-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawabe Y,Morio T,James JL,Prescott AR,Tanaka Y,Schaap P

更新日期：2009-04-28 00:00:00

abstract：:We have used wild-type and variants of the T-lymphoma cell line S49 to explore internalization and down-regulation of adenylate cyclase-linked beta-adrenergic receptors. Internalization was defined by the loss in "surface receptors" detected at 4 degrees C on intact cells by the antagonists [3H]CGP-12177 or [125I]iodo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahan LC,Koachman AM,Insel PA

更新日期：1985-01-01 00:00:00

abstract：:Brain src protooncogene is expressed in two forms, one identical to message in other tissues, and one containing an 18-nucleotide insert specific to brain. We have mapped mRNA for the two forms of src in rat brain with selective antisense oligonucleotide probes to the brain (src+) and peripheral (src-) forms. Fetal ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ross CA,Wright GE,Resh MD,Pearson RC,Snyder SH

更新日期：1988-12-01 00:00:00

abstract：:T-regulatory cells (Treg) and mast cells (MC) are abundant in colorectal cancer (CRC) tumors. Interaction between the two is known to promote immune suppression or loss of Treg functions and autoimmunity. Here, we demonstrate that in both human CRC and murine polyposis the outcome of this interaction is the generation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blatner NR,Bonertz A,Beckhove P,Cheon EC,Krantz SB,Strouch M,Weitz J,Koch M,Halverson AL,Bentrem DJ,Khazaie K

更新日期：2010-04-06 00:00:00

abstract：:We have isolated an Arabidopsis thaliana gene that codes for a receptor related to antifungal pathogenesis-related (PR) proteins. The PR5K gene codes for a predicted 665-amino acid polypeptide that comprises an extracellular domain related to the PR5 proteins, a central transmembrane-spanning domain, and an intracellu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Zafian P,Choudhary M,Lawton M

更新日期：1996-03-19 00:00:00

abstract：:Fast axoplasmic transport was studied in dorsal root ganglion cells of the cat. Proteins carried in the fast axoplasmic flow were labeled after an intraganglionic injection of (L)-[4,5-(3)H]leucine. The rate of transport was 380 +/- 26 mm/day in both the central and peripheral branches of the bifurcating axons that ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson LE,McClure WO

更新日期：1973-05-01 00:00:00

abstract：:Arc repressor forms a homodimer in which the subunits intertwine to create a single globular domain. To obtain Arc sequences that fold preferentially as heterodimers, variants with surface patches of excess positive or negative charge were designed. Several but not all oppositely charged sequence pairs showed preferen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nohaile MJ,Hendsch ZS,Tidor B,Sauer RT

更新日期：2001-03-13 00:00:00

abstract：:The visual images in the eyes contain much more information than the brain can process. An important selection mechanism is feature-based attention (FBA). FBA is best described by attention filters that specify precisely the extent to which items containing attended features are selectively processed and the extent to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun P,Chubb C,Wright CE,Sperling G

更新日期：2016-10-25 00:00:00

abstract：:The adult repertoire of antibody specificities is acquired in a developmentally programmed fashion that, in mouse and man, parallels the ordered rearrangement of a limited number of germ-line heavy chain variable region (VH) gene segments during development. It has been hypothesized that this developmental bias is a c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willems van Dijk K,Milner LA,Sasso EH,Milner EC

更新日期：1992-11-01 00:00:00

abstract：:To use recombinant DNA technology to functionally analyze mutations introduced into cloned eukaryotic genes, a rapid procedure is necessary to assay the steps along the gene expression pathway. Since cloned rat insulin genes are not transcribed efficiently after transfection into various cell lines, I have asked wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lomedico PT

更新日期：1982-10-01 00:00:00

abstract：:The protein products of T4 bacteriophage genes 41, 43, 45, 44, and 62 have been purified to near homogeneity using an assay which measures their stimulation of DNA synthesis in a crude lysate of Escherichia coli cells in fected by an appropriate mutant phage. When all of these proteins and T4 gene 32 protein are incub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morris CF,Sinha NK,Alberts BM

更新日期：1975-12-01 00:00:00

abstract：:Hatchlings of the painted turtle (Chrysemys picta marginata) are unique as the only reptile and highest vertebrate life form known to tolerate the natural freezing of extracellular body fluids during winter hibernation. Turtles survived frequent exposures to temperatures as low as -6 degrees C to -8 degrees C in their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Storey KB,Storey JM,Brooks SP,Churchill TA,Brooks RJ

更新日期：1988-11-01 00:00:00

abstract：:We evaluated the formation and removal of (+-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene (BcPHDE)-DNA adducts in two Chinese hamster ovary (CHO) cell lines. One line of repair-proficient cells (MK42) carries a stable 150-fold amplification of the dihydrofolate reductase (DH...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carothers AM,Zhen W,Mucha J,Zhang YJ,Santella RM,Grunberger D,Bohr VA

更新日期：1992-12-15 00:00:00

abstract：:The kinetics and order of rearrangements in the gene complex encoding T-cell-receptor beta chains were studied by Southern blot hybridization in a collection of hybridomas derived from fetal thymocytes at various stages of ontogeny (day 14 to day 17). Our results show a steady increase in the frequency of rearranged b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Born W,Yagüe J,Palmer E,Kappler J,Marrack P

更新日期：1985-05-01 00:00:00

abstract：:Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devise...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aigner-Held R,Campbell RA,Daves GD Jr

更新日期：1979-12-01 00:00:00

abstract：:The patch-clamp technique allows currents to be recorded through single ion channels in patches of cell membrane in the tips of glass pipettes. When recording, voltage is typically applied across the membrane patch to drive ions through open channels and to probe the voltage-sensitivity of channel activity. In this st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gil Z,Silberberg SD,Magleby KL

更新日期：1999-12-07 00:00:00

abstract：:Inherited colorectal cancer syndromes in humans exhibit regional specificity for tumor formation. By using mice with germline mutations in the adenomatous polyposis coli gene (Apc) and/or DNA mismatch repair genes, we have analyzed the genetic control of tumor regionality in the mouse small intestine. In C57BL/6 mice ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haigis KM,Hoff PD,White A,Shoemaker AR,Halberg RB,Dove WF

更新日期：2004-06-29 00:00:00

abstract：:This article addresses the need for new data on indirect effects of natural and anthropogenic aerosol particles on atmospheric ice clouds. Simultaneous measurements of the concentration and composition of tropospheric aerosol particles capable of initiating ice in cold (cirrus) clouds are reported. Measurements suppor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeMott PJ,Cziczo DJ,Prenni AJ,Murphy DM,Kreidenweis SM,Thomson DS,Borys R,Rogers DC

更新日期：2003-12-09 00:00:00

abstract：:Oligomerization plays a critical role in shaping the light-harvesting properties of many photosynthetic pigment-protein complexes, but a detailed understanding of this process at the level of individual pigments is still lacking. To study the effects of oligomerization, we designed a single-molecule approach to probe ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Q,Moerner WE

更新日期：2015-11-10 00:00:00