Differential transport of protein in axons: comparison between the sciatic nerve and dorsal columns of cats.

abstract：:Recent archaeological discoveries have revealed that pigment use, beads, engravings, and sophisticated stone and bone tools were already present in southern Africa 75,000 y ago. Many of these artifacts disappeared by 60,000 y ago, suggesting that modern behavior appeared in the past and was subsequently lost before be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： d'Errico F,Backwell L,Villa P,Degano I,Lucejko JJ,Bamford MK,Higham TF,Colombini MP,Beaumont PB

更新日期：2012-08-14 00:00:00

abstract：:Graphene oxide (GO), the main precursor of graphene-based materials made by solution processing, is known to be very stiff. Indeed, it has a Young's modulus comparable to steel, on the order of 300 GPa. Despite its very high stiffness, we show here that GO is superflexible. We quantitatively measure the GO bending rig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poulin P,Jalili R,Neri W,Nallet F,Divoux T,Colin A,Aboutalebi SH,Wallace G,Zakri C

更新日期：2016-10-04 00:00:00

abstract：:The c-myc oncogene has been implicated in a wide spectrum of B-cell neoplasias. In normal cells, the level of expression of the c-myc gene correlates with growth status. In the present study, we examined the effect of receptor-mediated inhibition of growth on c-myc expression in a B-cell lymphoma. The murine lymphoma ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCormack JE,Pepe VH,Kent RB,Dean M,Marshak-Rothstein A,Sonenshein GE

更新日期：1984-09-01 00:00:00

abstract：:We report that oxytocin (Oxt) receptors (Oxtrs), on stimulation by the ligand Oxt, translocate into the nucleus of osteoblasts, implicating this process in the action of Oxt on osteoblast maturation. Sequential immunocytochemistry of intact cells or isolated nucleoplasts stripped of the outer nuclear membrane showed p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Benedetto A,Sun L,Zambonin CG,Tamma R,Nico B,Calvano CD,Colaianni G,Ji Y,Mori G,Grano M,Lu P,Colucci S,Yuen T,New MI,Zallone A,Zaidi M

更新日期：2014-11-18 00:00:00

abstract：:Forests play a key role in the carbon balance of terrestrial ecosystems. One of the main uncertainties in global change predictions lies in how the spatiotemporal dynamics of forest productivity will be affected by climate warming. Here we show an increasing influence of climate on the spatial variability of tree grow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shestakova TA,Gutiérrez E,Kirdyanov AV,Camarero JJ,Génova M,Knorre AA,Linares JC,Resco de Dios V,Sánchez-Salguero R,Voltas J

更新日期：2016-01-19 00:00:00

abstract：:We show that it is possible to direct particles entrained in a fluid along trajectories much like rays of light in classical optics. A microstructured, asymmetric post array forms the core hydrodynamic element and is used as a building block to construct microfluidic metamaterials and to demonstrate refractive, focusi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morton KJ,Loutherback K,Inglis DW,Tsui OK,Sturm JC,Chou SY,Austin RH

更新日期：2008-05-27 00:00:00

abstract：:The N,N'-diacetyllactosediamine (lacdiNAc) pathway of complex-type oligosaccharide synthesis is controlled by a UDP-GalNAc:GlcNAc beta-R beta 1-->4-N-acetylgalac-tesaminyltransferase (beta 4-GalNAcT) that acts analogously to the common UDP-Gal:GlcNAc beta-R beta 1-->4-galactosyltransferase (beta 4-GalT). LacdiNAc-base...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neeleman AP,van de Eijnden DH

更新日期：1996-09-17 00:00:00

abstract：:One approach to understanding common human diseases is to determine the genetic defects responsible for similar diseases in animal models and place those defective genes in their corresponding biochemical pathways. Our laboratory is working with an animal model for human rheumatoid arthritis called collagen-induced ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McIndoe RA,Bohlman B,Chi E,Schuster E,Lindhardt M,Hood L

更新日期：1999-03-02 00:00:00

abstract：:Use-dependent long-term changes of neuronal response properties must be gated to prevent irrelevant activity from inducing inappropriate modifications. Here we test the hypothesis that local network dynamics contribute to such gating. As synaptic modifications depend on temporal contiguity between presynaptic and post...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galuske RAW,Munk MHJ,Singer W

更新日期：2019-11-12 00:00:00

abstract：:Low-temperature flash photolysis with IR and visible spectroscopy was used to probe the influence of the distal histidine His-64(E7) of sperm-whale myoglobin (Mb) on the orientation of bound carbon monoxide (CO) and on the kinetics of CO rebinding. The synthesis and high-level expression of a sperm-whale myoglobin gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braunstein D,Ansari A,Berendzen J,Cowen BR,Egeberg KD,Frauenfelder H,Hong MK,Ormos P,Sauke TB,Scholl R

更新日期：1988-11-01 00:00:00

abstract：:To initiate transcription from specific promoters, the bacterial RNA polymerase (RNAP) core enzyme must associate with the initiation factor sigma, which contains determinants that allow sequence-specific interactions with promoter DNA. Most bacteria contain several sigma factors, each of which directs recognition of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yuan AH,Nickels BE,Hochschild A

更新日期：2009-04-21 00:00:00

abstract：:Stress-induced impairments in extinction learning are believed to sustain posttraumatic stress disorder (PTSD). Noradrenergic signaling may contribute to extinction impairments by modulating medial prefrontal cortex (mPFC) circuits involved in fear regulation. Here we demonstrate that aversive fear conditioning rapidl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fitzgerald PJ,Giustino TF,Seemann JR,Maren S

更新日期：2015-07-14 00:00:00

abstract：:Mass cytometry enables an unprecedented number of parameters to be measured in individual cells at a high throughput, but the large dimensionality of the resulting data severely limits approaches relying on manual "gating." Clustering cells based on phenotypic similarity comes at a loss of single-cell resolution and o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shekhar K,Brodin P,Davis MM,Chakraborty AK

更新日期：2014-01-07 00:00:00

abstract：:In yeasts and mammals, PEX10 encodes an integral membrane protein with a C3HC4 RING finger motif in its C-terminal domain and is required for peroxisome biogenesis and matrix protein import. In humans, its dysfunction in peroxisome biogenesis leads to severe Zellweger Syndrome and infantile Refsum disease. Here we sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schumann U,Wanner G,Veenhuis M,Schmid M,Gietl C

更新日期：2003-08-05 00:00:00

abstract：:Mouse serosal mast cells (SMCs) and Kirsten sarcoma virus-immortalized mast cells store large amounts of mast cell carboxypeptidase A and serine proteases in their secretory granules. Secretory granule proteins from 2.6 x 10(6) purified SMCs were separated by NaDodSO4/PAGE, trans-blotted to poly(vinylidine difluoride)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds DS,Stevens RL,Lane WS,Carr MH,Austen KF,Serafin WE

更新日期：1990-04-01 00:00:00

abstract：:Progress in ecosystem service science has been rapid, and there is now a healthy appetite among key public and private sector decision makers for this science. However, changing policy and management is a long-term project, one that raises a number of specific practical challenges. One impediment to broad adoption of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polasky S,Tallis H,Reyers B

更新日期：2015-06-16 00:00:00

abstract：:We have used wild-type and variants of the T-lymphoma cell line S49 to explore internalization and down-regulation of adenylate cyclase-linked beta-adrenergic receptors. Internalization was defined by the loss in "surface receptors" detected at 4 degrees C on intact cells by the antagonists [3H]CGP-12177 or [125I]iodo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahan LC,Koachman AM,Insel PA

更新日期：1985-01-01 00:00:00

abstract：:Zn2+ is critical for the functional and structural integrity of cells and contributes to a number of important processes including gene expression. It has been shown that NO exogenously applied via NO donors resulting in nitrosative stress leads to cytoplasmic Zn2+ release from the zinc storing protein metallothionein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spahl DU,Berendji-Grün D,Suschek CV,Kolb-Bachofen V,Kröncke KD

更新日期：2003-11-25 00:00:00

abstract：:The kinetics and order of rearrangements in the gene complex encoding T-cell-receptor beta chains were studied by Southern blot hybridization in a collection of hybridomas derived from fetal thymocytes at various stages of ontogeny (day 14 to day 17). Our results show a steady increase in the frequency of rearranged b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Born W,Yagüe J,Palmer E,Kappler J,Marrack P

更新日期：1985-05-01 00:00:00

abstract：:We have synthesized 175-nucleotide-long probes for the DNA of human histocompatibility antigens HLA-DR alpha and beta by extending on poly(A)+ mRNA from B-cell lines with short synthetic deoxyribonucleotide primers complementary to the predicted nucleotide sequence of the NH2 terminus of both polypeptides. The synthes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das HK,Biro PA,Cohen SN,Erlich HA,von Gabain A,Lawrance SK,Lemaux PG,McDevitt HO,Peterlin BM,Schulz MF,Sood AK,Weissman SM

更新日期：1983-03-01 00:00:00

abstract：:A major part of the present understanding of the molecular basis of signal transduction has been gained from in vitro studies using classical biochemical methods. In this study, we used 31P NMR spectroscopy to investigate the response of live M2R mouse melanoma cells to stimulation by melanocyte-stimulating hormone (M...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Degani H,DeJordy JO,Salomon Y

更新日期：1991-02-15 00:00:00

abstract：:Climate change is expected to alter the distribution and abundance of many species. Predictions of climate-induced population extinctions are supported by geographic range shifts that correspond to climatic warming, but few extinctions have been linked mechanistically to climate change. Here we show that extinctions o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLaughlin JF,Hellmann JJ,Boggs CL,Ehrlich PR

更新日期：2002-04-30 00:00:00

abstract：:A large number of primary petite (rho-) clones were isolated after ethidium bromide mutagenesis of various grande (rho+) strains of S. cerevisiae that contained the mitochondrial genetic markers, CR, ER, OIR (or OIIR), and PR. From the frequency of coretention of markers in the petites, we have deduced a probable circ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boltin-Fukuhara M,Fukuhara H

更新日期：1976-12-01 00:00:00

abstract：:A method is described for the production of hybridoma cell lines secreting antibodies directed against the polyoma virus tumor antigens (T-Ags). Ten cloned lines have been established by this procedure and designated alpha Py Cl and C4, with activities against all three T-Ags; alpha Py LT1, -4 and -7, with activities ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dilworth SM,Griffin BE

更新日期：1982-02-01 00:00:00

abstract：:The Rhizobium common nod gene products NodABC are involved in the synthesis of the core lipochitooligosaccharide (Nod factor) structure, whereas the products of the host-specific nod genes are necessary for diverse structural modifications, which vary in different Rhizobium species. The sulfate group attached to the R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schultze M,Staehelin C,Röhrig H,John M,Schmidt J,Kondorosi E,Schell J,Kondorosi A

更新日期：1995-03-28 00:00:00

abstract：:Increased intestinal permeability has been observed in numerous human autoimmune diseases, including type-1 diabetes (T1D) and its' animal model, the BB-wor diabetic prone rat. We have recently described zonulin, a protein that regulates intercellular tight junctions. The objective of this study was to establish wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watts T,Berti I,Sapone A,Gerarduzzi T,Not T,Zielke R,Fasano A

更新日期：2005-02-22 00:00:00

abstract：:ClpB is a ring-forming, ATP-dependent protein disaggregase that cooperates with the cognate Hsp70 system to recover functional protein from aggregates. How ClpB harnesses the energy of ATP binding and hydrolysis to facilitate the mechanical unfolding of previously aggregated, stress-damaged proteins remains unclear. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biter AB,Lee S,Sung N,Tsai FT

更新日期：2012-07-31 00:00:00

abstract：:A duplex 21 nucleotide small interfering RNA (siRNA) against human Keap1 is described that represents a unique class of cancer chemopreventive agent. This siRNA can knockdown Keap1 mRNA and thereby relieve negative regulation of nuclear factor erythroid 2 p45-related factor 2 (Nrf2)-mediated gene expression. The siRNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Devling TW,Lindsay CD,McLellan LI,McMahon M,Hayes JD

更新日期：2005-05-17 00:00:00

abstract：:Pure water in a highly (1)H spin-polarized state is proposed as a contrast-agent-free contrast agent to visualize its macroscopic evolution in aqueous media by MRI. Remotely enhanced liquids for image contrast (RELIC) utilizes a (1)H signal of water that is enhanced outside the sample in continuous-flow mode and immed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCarney ER,Armstrong BD,Lingwood MD,Han S

更新日期：2007-02-06 00:00:00

abstract：:Long-term memory relies on modulation of synaptic connections in response to experience. This plasticity involves trafficking of AMPA receptors (AMPAR) and alteration of spine morphology. Arc, a gene induced by synaptic activity, mediates the endocytosis of AMPA receptors and is required for both long-term and homeost...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peebles CL,Yoo J,Thwin MT,Palop JJ,Noebels JL,Finkbeiner S

更新日期：2010-10-19 00:00:00