Arc regulates spine morphology and maintains network stability in vivo.

abstract：:We report that diverse species of bacteria encode a type IB DNA topoisomerase that resembles vaccinia virus topoisomerase. Deinococcus radiodurans topoisomerase IB (DraTopIB), an exemplary member of this family, relaxes supercoiled DNA in the absence of a divalent cation or ATP. DraTopIB has a compact size (346 aa) an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krogh BO,Shuman S

更新日期：2002-02-19 00:00:00

abstract：:Various compounds that affect signal transduction regulate the relative utilization of alternative processing pathways for the beta-amyloid precursor protein (beta APP) in intact cells, increasing the production of nonamyloidogenic soluble beta APP (s beta APP) and decreasing that of amyloidogenic beta-amyloid peptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu H,Sweeney D,Greengard P,Gandy S

更新日期：1996-04-30 00:00:00

abstract：:Serum levels of apolipoprotein CIII (apoCIII) are increased in type 1 diabetic patients, and when β cells are exposed to these diabetic sera, apoptosis occurs, an effect abolished by an antibody against apoCIII. We have investigated the BB rat, an animal model that develops a human-like type 1 diabetes, and found that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmberg R,Refai E,Höög A,Crooke RM,Graham M,Olivecrona G,Berggren PO,Juntti-Berggren L

更新日期：2011-06-28 00:00:00

abstract：:A human craniofacial cDNA library was screened with a degenerate oligonucleotide probe based on the conserved third helix of homeobox genes. From this screening, we identified a homeobox gene, H6, which shared only 57-65% amino acid identity to previously reported homeodomains. H6 was physically mapped to the 4p16.1 r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stadler HS,Padanilam BJ,Buetow K,Murray JC,Solursh M

更新日期：1992-12-01 00:00:00

abstract：:Estrogen receptor alpha (ERalpha) plays a major role in the regulation of neuroendocrine functions and behaviors by estrogens. Although the generation of ERalpha knockout mice advanced our knowledge of ERalpha functions, gene deletion using this method is global and potentially confounded by developmental consequences...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musatov S,Chen W,Pfaff DW,Kaplitt MG,Ogawa S

更新日期：2006-07-05 00:00:00

abstract：:Energy relaxation in light-harvesting complexes has been extensively studied by various ultrafast spectroscopic techniques, the fastest processes being in the sub-100-fs range. At the same time, much slower dynamics have been observed in individual complexes by single-molecule fluorescence spectroscopy (SMS). In this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malý P,Gruber JM,Cogdell RJ,Mančal T,van Grondelle R

更新日期：2016-03-15 00:00:00

abstract：:Fluorescence photobleaching recovery was used to measure the lateral diffusion coefficient and mobile fraction of surface immunoglobulin (sIg), Thy-1 antigen, and a lipid probe in the plasma membrane of mouse lymphocytes. The lipid probe (3,3'-dioctadecylindocarbocyanine) had a mean (+/-SD) diffusion coefficient of (1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dragsten P,Henkart P,Blumenthal R,Weinstein J,Schlessinger J

更新日期：1979-10-01 00:00:00

abstract：:Managing excess nutrients remains a major obstacle to improving ecosystem service benefits of urban waters. To inform more ecologically based landscape nutrient management, we compared watershed inputs, outputs, and retention for nitrogen (N) and phosphorus (P) in seven subwatersheds of the Mississippi River in St. Pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hobbie SE,Finlay JC,Janke BD,Nidzgorski DA,Millet DB,Baker LA

更新日期：2017-04-18 00:00:00

abstract：:A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rossi JM,Burke DT,Leung JC,Koos DS,Chen H,Tilghman SM

更新日期：1992-03-15 00:00:00

abstract：:Glioblastoma multiforme (GBM; grade IV astrocytoma) is the most prevalent and aggressive form of primary brain cancer. A subpopulation of multipotent cells termed GBM cancer stem cells (CSCs) play a critical role in tumor initiation, tumor maintenance, metastasis, drug resistance, and recurrence following surgery. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lucki NC,Villa GR,Vergani N,Bollong MJ,Beyer BA,Lee JW,Anglin JL,Spangenberg SH,Chin EN,Sharma A,Johnson K,Sander PN,Gordon P,Skirboll SL,Wurdak H,Schultz PG,Mischel PS,Lairson LL

更新日期：2019-03-26 00:00:00

abstract：:In multiple binding of ligands to a protein, the binding sites may seem to behave as if they are partitioned equally between two modalities. This paper analyzes three different molecular situations in which two actual assemblages appear: (i) two classes of sites exist at the outset in the ligand-free macromolecule; (i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klotz IM,Hunston DL

更新日期：1977-11-01 00:00:00

abstract：:Throughout the world, efforts are under way to restore watersheds, but restoration planning rarely accounts for future climate change. Using a series of linked models of climate, land cover, hydrology, and salmon population dynamics, we investigated the impacts of climate change on the effectiveness of proposed habita...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Battin J,Wiley MW,Ruckelshaus MH,Palmer RN,Korb E,Bartz KK,Imaki H

更新日期：2007-04-17 00:00:00

abstract：:Activation of phospholipase Cgamma1 (PLCgamma1) by vascular endothelial growth factor receptor-2 (VEGFR-2) in endothelial cells in part is responsible for angiogenesis in vivo. The cellular mechanisms exerting negative control over PLCgamma1 activation, however, remain unaddressed. Here by using in vitro and in vivo b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh AJ,Meyer RD,Navruzbekov G,Shelke R,Duan L,Band H,Leeman SE,Rahimi N

更新日期：2007-03-27 00:00:00

abstract：:C factor, an intercellular signaling protein, is required for aggregation and sporulation of the social bacterium, Myxococcus xanthus. We report that C factor, which normally is associated with the cell surface, provides input to the Frz signal transduction cascade. Elements of this cascade have sequence homology to b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Søgaard-Andersen L,Kaiser D

更新日期：1996-04-02 00:00:00

abstract：:The underpinnings of STAT3 hyperphosphorylation resulting in enhanced signaling and cancer progression are incompletely understood. Loss-of-function mutations of enzymes that dephosphorylate STAT3, such as receptor protein tyrosine phosphatases, which are encoded by the PTPR gene family, represent a plausible mechanis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lui VW,Peyser ND,Ng PK,Hritz J,Zeng Y,Lu Y,Li H,Wang L,Gilbert BR,General IJ,Bahar I,Ju Z,Wang Z,Pendleton KP,Xiao X,Du Y,Vries JK,Hammerman PS,Garraway LA,Mills GB,Johnson DE,Grandis JR

更新日期：2014-01-21 00:00:00

abstract：:We report on the location of the chain part of the retinylidene chromophore in the projected density of bacteriorhodopsin as determined by neutron diffraction from the two-dimensional purple membrane lattice. For this purpose, partially deuterated retinal was synthesized containing 10 deuterons at positions C-8, C-10,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seiff F,Wallat I,Ermann P,Heyn MP

更新日期：1985-05-01 00:00:00

abstract：:Dominantly inherited mutations in the genes encoding presenilins (PS) and the amyloid precursor protein (APP) are the major causes of familial Alzheimer's disease (AD). The prevailing view of AD pathogenesis posits that accumulation of beta-amyloid (Abeta) peptides, particularly Abeta42, is the central event triggerin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen J,Kelleher RJ 3rd

更新日期：2007-01-09 00:00:00

abstract：:A topological model of the DNA binding site for the cyclic AMP receptor protein (CRP) is presented. A consensus sequence drawn from the known CRP binding sites has several symmetrical subregions that are spatially resolved onto different faces of the DNA helix. Consideration of available biochemical and genetic data s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Neill MC,Amass K,de Crombrugghe B

更新日期：1981-04-01 00:00:00

abstract：:The yeast AAA-ATPase Cdc48 and the ubiquitin fusion degradation (UFD) proteins play important, evolutionarily conserved roles in ubiquitin dependent protein degradation. The N-terminal domain of Cdc48 interacts with substrate-recruiting cofactors, whereas the C terminus of Cdc48 binds to proteins such as Ufd3 that pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao G,Li G,Schindelin H,Lennarz WJ

更新日期：2009-09-22 00:00:00

abstract：:Surface Ig (sIg) of follicular lymphoma (FL) is vital for tumor cell survival. We found previously that the Ig in FL is unusual, because the variable region genes carry sequence motifs for N-glycan addition. These are introduced by somatic mutation and are tumor specific. Unexpectedly, added glycans terminate at high ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coelho V,Krysov S,Ghaemmaghami AM,Emara M,Potter KN,Johnson P,Packham G,Martinez-Pomares L,Stevenson FK

更新日期：2010-10-26 00:00:00

abstract：:Modulation of cholesterol absorption in the intestine, the primary site of dietary cholesterol uptake in humans, can have profound clinical implications. We have undertaken a reverse genetic approach by disrupting putative cholesterol processing genes in zebrafish larvae by using morpholino (MO) antisense oligonucleot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smart EJ,De Rose RA,Farber SA

更新日期：2004-03-09 00:00:00

abstract：:The laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) gene from Neurospora crassa was cloned and part of its nucleotide sequence corresponding to the carboxyl-terminal region of the protein has been determined. The gene was cloned by cDNA synthesis with a laccase-specific synthetic deoxyundecanucleotide as prim...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Germann UA,Lerch K

更新日期：1986-12-01 00:00:00

abstract：:Autocatalytic chemical reactions are widely studied as models of biological processes and to better understand the origins of life on Earth. Minimal self-reproducing amphiphiles have been developed in this context and as an approach to de novo "bottom-up" synthetic protocells. How chemicals come together to produce li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ortega-Arroyo J,Bissette AJ,Kukura P,Fletcher SP

更新日期：2016-10-04 00:00:00

abstract：:In this paper, we report the transcriptional organization of a 450-kb gene cluster in Xq28, flanked by the glucose-6-phosphate dehydrogenase and the color vision genes. CpG islands previously identified and mapped to distal Xq28 have helped in construction of a continuous contig of cosmids and in identification of cDN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bione S,Tamanini F,Maestrini E,Tribioli C,Poustka A,Torri G,Rivella S,Toniolo D

更新日期：1993-12-01 00:00:00

abstract：:The skeletal muscle L-type Ca2+ channel is a complex of five subunits that is specifically localized in the triad. Its primary function is the rapid activation of Ca2+ release from cytoplasmic stores in a process called excitation-contraction coupling. To study the role of alpha1S-beta1a interactions in the incorporat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neuhuber B,Gerster U,Döring F,Glossmann H,Tanabe T,Flucher BE

更新日期：1998-04-28 00:00:00

abstract：:Sialic acid-recognizing Ig-like lectins (Siglecs) are signaling receptors that modulate immune responses, and are targeted for interactions by certain pathogens. We describe two primate Siglecs that were rendered nonfunctional by single genetic events during hominin evolution after our common ancestor with the chimpan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Mitra N,Secundino I,Banda K,Cruz P,Padler-Karavani V,Verhagen A,Reid C,Lari M,Rizzi E,Balsamo C,Corti G,De Bellis G,Longo L,NISC Comparative Sequencing Program.,Beggs W,Caramelli D,Tishkoff SA,Hayakawa T,Gree

更新日期：2012-06-19 00:00:00

abstract：:Normal as well as Rous sarcoma virus-infected chicken pectoral and chicken embryo fibroblasts proliferate actively in a plasma containing medium of physiological ion concentrations (Ca2+, 1.2 mM; Mg2+, 0.7 mM). Reduction of medium calcium and magnesium concentrations is necessary to achieve selective quiescence of nor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balk SD

更新日期：1980-11-01 00:00:00

abstract：:When fibers of fibrin clots or fragments of fibrinogen pellets are negatively stained they exhibit in the electron microscope characteristic patterns of cross-striations or bands. Those found in pellet material are indistinguishable from those seen in thrombin-induced fibrin fibers. The pattern seen in fibrin from bov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams RC

更新日期：1983-03-01 00:00:00

abstract：:The inability to perceive and coordinate both internal and external signals that function to regulate cellular growth and proliferation is a hallmark of oncogenic transformation. To examine the effects of the v-mos oncogene on distinct signal transduction pathways, the 6m2 cell line was used, in which expression of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamilton BJ,DeFranco D

更新日期：1989-01-01 00:00:00

abstract：:Nanoparticles are increasingly used in various fields, including biomedicine and electronics. One application utilizes the opacifying effect of nano-TiO(2), which is frequently used as pigment in cosmetics. Although TiO(2) is believed to be biologically inert, an emerging literature reports increased incidence of resp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yazdi AS,Guarda G,Riteau N,Drexler SK,Tardivel A,Couillin I,Tschopp J

更新日期：2010-11-09 00:00:00