Metabolism of Alzheimer beta-amyloid precursor protein: regulation by protein kinase A in intact cells and in a cell-free system.

abstract：:Glycoprotein Ib is a surface membrane glycoprotein of platelets that functions as a receptor for von Willebrand factor. It is a heterodimer composed of an alpha and a beta chain linked by a disulfide bond(s). A phage lambda gt11 cDNA expression library prepared from mRNA from a human erythroleukemia cell line, HEL, wa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopez JA,Chung DW,Fujikawa K,Hagen FS,Papayannopoulou T,Roth GJ

更新日期：1987-08-01 00:00:00

abstract：:J-domain chaperones are involved in the efficient handover of misfolded/partially folded proteins to Hsp70 but also function independently to protect against cell death. Due to their high flexibility, the mechanism by which they regulate the Hsp70 cycle and how specific substrate recognition is performed remains unkno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karamanos TK,Tugarinov V,Clore GM

更新日期：2019-10-22 00:00:00

abstract：:The mechanism of phosphosylation and dephosphorylation of spectrin from human erythrocyte membranes has been examined under closely physiological conditions. The results support the hypothesis that spectrin is an autophosphorylating and dephosphorylating system. (i) Extraction from ghosts of up to 85% of the kinase (c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Imhof BA,Acha-Orbea HJ,Libermann TA,Reber BF,Lanz JH,Winterhalter KH,Birchmeier W

更新日期：1980-06-01 00:00:00

abstract：:Administration of epidermal growth factor (EGF) to neonatal mice resulted in rapid tyrosine phosphorylation of a number of specific substrates in liver, kidney, lung, bladder, skin, and brain as detected by Western blot analysis of tissue homogenates with anti-phosphotyrosine antibodies. In the liver, three prominent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donaldson RW,Cohen S

更新日期：1992-09-15 00:00:00

abstract：:Lipid droplets (LDs) are a conserved feature of most organisms. Vertebrate adipocytes have evolved to efficiently store and release lipids for the whole organism from a single droplet. Perilipin 1, the most abundant lipid-coat protein in adipocytes, plays a key role in regulating lipolysis. In other tissues such as li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel S,Yang W,Kozusko K,Saudek V,Savage DB

更新日期：2014-06-24 00:00:00

abstract：:Regional differences in neurons, astrocytes, oligodendrocytes, and microglia exist in the brain during health, and regional differences in the transcriptome may occur for each cell type during neurodegeneration. Multiple sclerosis (MS) is multifocal, and regional differences in the astrocyte transcriptome occur in exp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voskuhl RR,Itoh N,Tassoni A,Matsukawa MA,Ren E,Tse V,Jang E,Suen TT,Itoh Y

更新日期：2019-05-14 00:00:00

abstract：:Schaftoside and isoschaftoside are bioactive natural products widely distributed in higher plants including cereal crops and medicinal herbs. Their biosynthesis may be related with plant defense. However, little is known on the glycosylation biosynthetic pathway of these flavonoid di-C-glycosides with different sugar ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang ZL,Gao HM,Wang S,Zhang M,Chen K,Zhang YQ,Wang HD,Han BY,Xu LL,Song TQ,Yun CH,Qiao X,Ye M

更新日期：2020-12-01 00:00:00

abstract：:Control of transcription and enzyme activities are two interwoven regulatory systems essential for the function of a metabolic node. Saccharomyces cerevisiae strains differing in enzyme activities at the chorismate branch point of aromatic amino acid biosynthesis were constructed by recombinant DNA technology. Express...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krappmann S,Lipscomb WN,Braus GH

更新日期：2000-12-05 00:00:00

abstract：:The nucleolinus is an RNA-rich compartment, closely apposed to or embedded within the nucleolus. Discovered over 150 y ago, fewer than two dozen articles have been published on the nucleolinus, probably because complex histochemical stains are required for its visualization in the great majority of cells. The nucleoli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alliegro MA,Henry JJ,Alliegro MC

更新日期：2010-08-03 00:00:00

abstract：:Old Italian violins are routinely credited with playing qualities supposedly unobtainable in new instruments. These qualities include the ability to project their sound more effectively in a concert hall-despite seeming relatively quiet under the ear of the player-compared with new violins. Although researchers have l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fritz C,Curtin J,Poitevineau J,Tao FC

更新日期：2017-05-23 00:00:00

abstract：:In this study we compared the cellular concentrations and stability of the mRNA transcribed from the aprE (subtilisin) gene (a gene preferentially expressed in stationary growth phase) with those of a vegetative mRNA, succinate dehydrogenase (SDH) mRNA. The subtilisin transcript was shown to be at least 3 times more s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Resnekov O,Rutberg L,von Gabain A

更新日期：1990-11-01 00:00:00

abstract：:Polyketides represent an extremely diverse class of secondary metabolites often explored for their bioactive traits. These molecules are also attractive building blocks for chemical catalysis and polymerization. However, the use of polyketides in larger scale chemistry applications is stymied by limited titers and yie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markham KA,Palmer CM,Chwatko M,Wagner JM,Murray C,Vazquez S,Swaminathan A,Chakravarty I,Lynd NA,Alper HS

更新日期：2018-02-27 00:00:00

abstract：:The plant hormone indole-acetic acid (auxin) is essential for many aspects of plant development. Auxin-mediated growth regulation typically involves the establishment of an auxin concentration gradient mediated by polarly localized auxin transporters. The localization of auxin carriers and their amount at the plasma m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boutté Y,Jonsson K,McFarlane HE,Johnson E,Gendre D,Swarup R,Friml J,Samuels L,Robert S,Bhalerao RP

更新日期：2013-10-01 00:00:00

abstract：:The lentiviruses are associated with a wide range of chronic diseases in mammals. These include immunodeficiencies (such as HIV/AIDS in humans), malignancies, and lymphatic and neurological disorders in primates, felids, and a variety of wild and domesticated ungulates. Evolutionary analyses of the genomic sequences o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katzourakis A,Tristem M,Pybus OG,Gifford RJ

更新日期：2007-04-10 00:00:00

abstract：:A three-plasmid system for heterologous expression of 6-deoxyerythronolide B synthase (DEBS) has been developed to facilitate combinatorial biosynthesis of polyketides made by type I modular polyketide synthases (PKSs). The eryA PKS genes encoding the three DEBS subunits were individually cloned into three compatible ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xue Q,Ashley G,Hutchinson CR,Santi DV

更新日期：1999-10-12 00:00:00

abstract：:In the present work, we investigated the role of natural killer (NK) cells in combination therapy with oncolytic virus (OV) and bortezomib, a proteasome inhibitor. NK cells display rapid and potent immunity to metastatic and hematological cancers, and they overcome immunosuppressive effects of tumor microenvironment. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim Y,Yoo JY,Lee TJ,Liu J,Yu J,Caligiuri MA,Kaur B,Friedman A

更新日期：2018-05-08 00:00:00

abstract：:We have investigated the folding dynamics of Thermus thermophilus cytochrome c(552) by time-resolved fluorescence energy transfer between the heme and each of seven site-specific fluorescent probes. We have found both an equilibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies. De...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada S,Bouley Ford ND,Keller GE,Ford WC,Gray HB,Winkler JR

更新日期：2013-01-29 00:00:00

abstract：:The periaqueductal gray (PAG) and amygdala are known to be important for defensive responses, and many contemporary fear-conditioning models present the PAG as downstream of the amygdala, directing the appropriate behavior (i.e., freezing or fleeing). However, empirical studies of this circuitry are inconsistent and w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim EJ,Horovitz O,Pellman BA,Tan LM,Li Q,Richter-Levin G,Kim JJ

更新日期：2013-09-03 00:00:00

abstract：:Previous work has established stargazin and its related family of transmembrane AMPA receptor regulatory proteins (TARPs) as auxiliary subunits of AMPA receptors (AMPARs) that control synaptic strength both by targeting AMPARs to synapses through an intracellular PDZ-binding motif and by modulating their gating throug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milstein AD,Nicoll RA

更新日期：2009-07-07 00:00:00

abstract：:Reactive oxygen species, the by-products of oxidative energy metabolism, are considered a main proximate cause of aging. Accordingly, overexpression of the enzyme Cu-Zn superoxide dismutase 1 (SOD1) can lengthen lifespan of Drosophila melanogaster in the laboratory. However, the role of SOD1 as a main determinant of l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker JD,Parker KM,Sohal BH,Sohal RS,Keller L

更新日期：2004-03-09 00:00:00

abstract：:Neurons respond to sensory stimuli by altering the rate and temporal pattern of action potentials. These spike trains both encode and propagate information that guides behavior. Local inhibitory networks can affect the information encoded and propagated by neurons by altering correlations between different spike train...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giridhar S,Doiron B,Urban NN

更新日期：2011-04-05 00:00:00

abstract：:The massive forests of central Amazonia are often considered relatively resilient against climatic variation, but this view is challenged by the wildfires invoked by recent droughts. The impact of such fires that spread from pervasive sources of ignition may reveal where forests are less likely to persist in a drier f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flores BM,Holmgren M,Xu C,van Nes EH,Jakovac CC,Mesquita RCG,Scheffer M

更新日期：2017-04-25 00:00:00

abstract：:Long interspersed nuclear elements 1 (L1) are active retrotransposons that reside in many species, including humans and rodents. L1 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic location. We have tagged an active human L1 element (L1(RP)) with a gene encoding enh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prak ET,Dodson AW,Farkash EA,Kazazian HH Jr

更新日期：2003-02-18 00:00:00

abstract：:Progress in ecosystem service science has been rapid, and there is now a healthy appetite among key public and private sector decision makers for this science. However, changing policy and management is a long-term project, one that raises a number of specific practical challenges. One impediment to broad adoption of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polasky S,Tallis H,Reyers B

更新日期：2015-06-16 00:00:00

abstract：:Chromosome translocations between Ig (Ig) and non-Ig genes are frequently associated with B-cell lymphomas in humans and mice. The best characterized of these is c-myc/IgH translocation, which is associated with Burkitt's lymphoma. These translocations are caused by activation-induced cytidine deaminase (AID), which p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jankovic M,Robbiani DF,Dorsett Y,Eisenreich T,Xu Y,Tarakhovsky A,Nussenzweig A,Nussenzweig MC

更新日期：2010-01-05 00:00:00

abstract：:The excess proton in water, H(+)(aq), plays a fundamental role in aqueous solution chemistry. Its solution thermodynamic properties are essential to molecular descriptions of that chemistry and for validation of dynamical calculations. Within the quasichemical theory of solutions those thermodynamic properties are con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asthagiri D,Pratt LR,Kress JD

更新日期：2005-05-10 00:00:00

abstract：:The stereospecificity of the beta-adrenergic receptor of turkey erythrocyte ghosts was studied. Binding of the specific beta-adrenergic antagonist, propranolol, was found to occur exclusively with the l-stereoisomer. The stereospecificity of catecholamine binding was determined by assaying the ability of the catechola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Atlas D,Steer ML,Levitzki A

更新日期：1974-10-01 00:00:00

abstract：:Fisheries-induced evolution and its impact on the productivity of exploited fish stocks remains a highly contested research topic in applied fish evolution and fisheries science. Although many quantitative models assume that larger, more fecund fish are preferentially removed by fishing, there is no empirical evidence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutter DA,Suski CD,Philipp DP,Klefoth T,Wahl DH,Kersten P,Cooke SJ,Arlinghaus R

更新日期：2012-12-18 00:00:00

abstract：:Memory formation requires the placement of experienced events in the same order in which they appeared. A large body of evidence from human studies indicates that structures in the medial temporal lobe are critically involved in forming and maintaining such memories, and complementing evidence from lesion and electrop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paz R,Gelbard-Sagiv H,Mukamel R,Harel M,Malach R,Fried I

更新日期：2010-03-30 00:00:00

abstract：:The synthesis of cytoplasmic proteins from quiescent and serum-stimulated Swiss 3T3 cells was compared by two-dimensional polyacrylamide gel electrophoresis. Four new proteins of Mrs 26,000, 28,000, 45,000, and 47,000 designated N26, N28, N45, and N47, which were not detectable in quiescent cells, appeared 60 min afte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas G,Thomas G,Luther H

更新日期：1981-09-01 00:00:00