当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.

Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.

Abstract：

:A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Rossi JM,Burke DT,Leung JC,Koos DS,Chen H,Tilghman SM

doi

10.1073/pnas.89.6.2456

keywords:

subject

Has Abstract

pub_date

1992-03-15 00:00:00

pages

2456-60

issue

6

eissn

0027-8424

issn

1091-6490

journal_volume

89

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Direct visualization of receptors for thyrotropin-releasing hormone with a fluorescein-labeled analog.

    abstract：:Thyrotropin-releasing hormone (TRH) binds to specific receptors on GH4C1 pituitary tumor cells. A fluorescently labeled analog of TRH was synthesized by coupling pGlu-His-ProNH(CH2)6NH2 to fluorescein isothiocyanate. The fluorescein-labeled peptide (FL-TRH) stimulated prolactin synthesis and release by GH4C1 cultures ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.1.587

    authors： Halpern J,Hinkle PM

    更新日期：1981-01-01 00:00:00

  • Mitogenic leukoagglutinin from Phaseolus vulgaris binds to a pentasaccharide unit in N-acetyllactosamine-type glycoprotein glycans.

    abstract：:The carbohydrate binding specificity of leukoagglutinin (La; Phaseolus vulgaris isolectin L4) was studied by using quantitative precipitation and precipitation-inhibition. A series of purified glycopeptides and synthetic oligosaccharides were used as inhibitors. The minimal structural unit required for La binding was ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.5.1611

    authors： Hammarström S,Hammarström ML,Sundblad G,Arnarp J,Lönngren J

    更新日期：1982-03-01 00:00:00

  • Structure of a new DNA-binding domain which regulates pathogenesis in a wide variety of fungi.

    abstract：:WOPR-domain proteins are found throughout the fungal kingdom where they function as master regulators of cell morphology and pathogenesis. Genetic and biochemical experiments previously demonstrated that these proteins bind to specific DNA sequences and thereby regulate transcription. However, their primary sequence s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1410110111

    authors： Lohse MB,Rosenberg OS,Cox JS,Stroud RM,Finer-Moore JS,Johnson AD

    更新日期：2014-07-22 00:00:00

  • Three flies and three islands: parallel evolution in Drosophila.

    abstract：:Most organisms are evolutionary conservatives; they may subdivide their niches but tend to remain within them. Yet the fossil record shows many cases of breakthrough to a new mode of life. How may such evolutionary innovation be recognized at this time level? Three species of Drosophila have accomplished an innovation...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.9.3517

    authors： Carson HL

    更新日期：1974-09-01 00:00:00

  • Measurement of cortical elasticity in Drosophila melanogaster embryos using ferrofluids.

    abstract：:Many models of morphogenesis are forced to assume specific mechanical properties of cells, because the actual mechanical properties of living tissues are largely unknown. Here, we measure the rheology of epithelial cells in the cellularizing Drosophila embryo by injecting magnetic particles and studying their response...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1616659114

    authors： Doubrovinski K,Swan M,Polyakov O,Wieschaus EF

    更新日期：2017-01-31 00:00:00

  • Mode of interaction of TRIP13 AAA-ATPase with the Mad2-binding protein p31comet and with mitotic checkpoint complexes.

    abstract：:The AAA-ATPase thyroid hormone receptor interacting protein 13 (TRIP13), jointly with the Mad2-binding protein p31(comet), promotes the inactivation of the mitotic (spindle assembly) checkpoint by disassembling the mitotic checkpoint complex (MCC). This checkpoint system ensures the accuracy of chromosome segregation ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1515358112

    authors： Miniowitz-Shemtov S,Eytan E,Kaisari S,Sitry-Shevah D,Hershko A

    更新日期：2015-09-15 00:00:00

  • Geochemical evidence for combustion of hydrocarbons during the K-T impact event.

    abstract：:It has been proposed that extensive wildfires occurred after the Cretaceous-Tertiary (K-T) impact event. An abundance of soot and pyrosynthetic polycyclic aromatic hydrocarbons (pPAHs) in marine K-T boundary impact rocks (BIRs) have been considered support for this hypothesis. However, nonmarine K-T BIRs, from across ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 历史文章,杂志文章

    doi：10.1073/pnas.0813117106

    authors： Belcher CM,Finch P,Collinson ME,Scott AC,Grassineau NV

    更新日期：2009-03-17 00:00:00

  • The crystal structures of human steroidogenic factor-1 and liver receptor homologue-1.

    abstract：:Steroidogenic factor-1 (SF-1) and liver receptor homologue-1 (LRH-1) belong to the fushi tarazu factor 1 subfamily of nuclear receptors. SF-1 is an essential factor for sex determination during development and regulates adrenal and gonadal steroidogenesis in the adult, whereas LRH-1 is a critical factor for developmen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0409482102

    authors： Wang W,Zhang C,Marimuthu A,Krupka HI,Tabrizizad M,Shelloe R,Mehra U,Eng K,Nguyen H,Settachatgul C,Powell B,Milburn MV,West BL

    更新日期：2005-05-24 00:00:00

  • N-terminal domain of human Hsp90 triggers binding to the cochaperone p23.

    abstract：:The molecular chaperone Hsp90 is a protein folding machine that is conserved from bacteria to man. Human, cytosolic Hsp90 is dedicated to folding of chiefly signal transduction components. The chaperoning mechanism of Hsp90 is controlled by ATP and various cochaperones, but is poorly understood and controversial. Here...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1011867108

    authors： Karagöz GE,Duarte AM,Ippel H,Uetrecht C,Sinnige T,van Rosmalen M,Hausmann J,Heck AJ,Boelens R,Rüdiger SG

    更新日期：2011-01-11 00:00:00

  • Homeostatic mechanisms regulate distinct aspects of cortical circuit dynamics.

    abstract：:Homeostasis is indispensable to counteract the destabilizing effects of Hebbian plasticity. Although it is commonly assumed that homeostasis modulates synaptic strength, membrane excitability, and firing rates, its role at the neural circuit and network level is unknown. Here, we identify changes in higher-order netwo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1918368117

    authors： Wu YK,Hengen KB,Turrigiano GG,Gjorgjieva J

    更新日期：2020-09-29 00:00:00

  • Parallel-up structure evidences the molecular directionality during biosynthesis of bacterial cellulose.

    abstract：:The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and microdiffraction-tilting electron crystallographic analysis. Microdiffraction investigation of nascent bacterial cellulose microfibrils showed...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.17.9091

    authors： Koyama M,Helbert W,Imai T,Sugiyama J,Henrissat B

    更新日期：1997-08-19 00:00:00

  • Effects of glycyl-L-glutamine and methylprednisolone on maintenance of acetylcholinesterase of transected rat sciatic nerves.

    abstract：:Under anesthesia with sodium pentobarbital, the sciatic nerves of rats were transected bilaterally, and a catheter was inserted into the central end of the left renal artery. After an initial flush, an Alzet pump was attached to the catheter, containing various concentrations of glycyl-L-glutamine (Gly-Gln), methylpre...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.23.9495

    authors： Koelle GB,Han MS

    更新日期：1990-12-01 00:00:00

  • What drives the translocation of proteins?

    abstract：:We propose that protein translocation across membranes is driven by biased random thermal motion. This "Brownian ratchet" mechanism depends on chemical asymmetries between the cis and trans sides of the membrane. Several mechanisms could contribute to rectifying the thermal motion of the protein, such as binding and d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.9.3770

    authors： Simon SM,Peskin CS,Oster GF

    更新日期：1992-05-01 00:00:00

  • A single-stranded DNA-binding protein from Crithidia fasciculata recognizes the nucleotide sequence at the origin of replication of kinetoplast DNA minicircles.

    abstract：:A sequence-specific single-stranded DNA-binding protein from the trypanosomatid protozoan Crithidia fasciculata binds to a sequence of 12 nucleotides located at the origin of replication of kinetoplast DNA minicircles. This sequence, termed the universal minicircle sequence (UMS), is conserved in the kinetoplast DNA m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.15.6891

    authors： Tzfati Y,Abeliovich H,Kapeller I,Shlomai J

    更新日期：1992-08-01 00:00:00

  • Supercooled spin liquid state in the frustrated pyrochlore Dy2Ti2O7.

    abstract：:A "supercooled" liquid develops when a fluid does not crystallize upon cooling below its ordering temperature. Instead, the microscopic relaxation times diverge so rapidly that, upon further cooling, equilibration eventually becomes impossible and glass formation occurs. Classic supercooled liquids exhibit specific id...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1511006112

    authors： Kassner ER,Eyvazov AB,Pichler B,Munsie TJ,Dabkowska HA,Luke GM,Davis JC

    更新日期：2015-07-14 00:00:00

  • Sulfhydryl oxidation down-regulates T-cell signaling and inhibits tyrosine phosphorylation of phospholipase C gamma 1.

    abstract：:Early events in both T-cell receptor (CD3)- and CD4-induced signal transduction pathways include tyrosine phosphorylation of protein substrates, the generation of phosphatidylinositol-phosphate breakdown products, and the mobilization of intracellular Ca2+. Oxidative stress in T cells mediated by sulfhydryl-reactive n...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.1.300

    authors： Kanner SB,Kavanagh TJ,Grossmann A,Hu SL,Bolen JB,Rabinovitch PS,Ledbetter JA

    更新日期：1992-01-01 00:00:00

  • Influence of delayed immune reactions on human epidermal keratinocytes.

    abstract：:The epidermal changes that occur in human cutaneous immune responses have been investigated in the tuberculin reaction and in the lesions of tuberculoid and lepromatous leprosy and cutaneous leishmaniasis. In each situation, there was a dermal accumulation of monocytes and T cells, and the epidermis exhibited thickeni...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.10.3469

    authors： Kaplan G,Witmer MD,Nath I,Steinman RM,Laal S,Prasad HK,Sarno EN,Elvers U,Cohn ZA

    更新日期：1986-05-01 00:00:00

  • Identification of the Fc gamma receptor class I binding site in human IgG through the use of recombinant IgG1/IgG2 hybrid and point-mutated antibodies.

    abstract：:To characterize the region on human IgG1 responsible for its high-affinity interaction with the human Fc gamma receptor class I (Fc gamma RI), we have analyzed the binding properties of a series of genetically engineered chimeric antidinitrophenyl antibodies with identical murine antibody combining sites and hybrid Ig...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.20.9036

    authors： Chappel MS,Isenman DE,Everett M,Xu YY,Dorrington KJ,Klein MH

    更新日期：1991-10-15 00:00:00

  • Vacuolar H(+)-pumping ATPase variable transport coupling ratio controlled by pH.

    abstract：:The eukaryote endomembrane system contains a class of H(+)-pumping ATPase (H(+)-ATPases) of the vacuolar type (V-ATPases) that are responsible for the acidification of organelles. Their action is critical to numerous physiological processes, but the regulatory mechanisms that may control activity are not yet fully und...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.18.8547

    authors： Davies JM,Hunt I,Sanders D

    更新日期：1994-08-30 00:00:00

  • E1A and E1B proteins inhibit inflammation induced by adenovirus.

    abstract：:Replication-defective human adenovirus (Ad) group C transducing vectors, most of which have the E1A, E1B, and E3 genes deleted, are highly inflammatory despite the fact that the parental viruses typically cause subclinical or mild infections. To investigate this paradox, the roles that the E1A, E1B, and E3 genes play ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0303709101

    authors： Schaack J,Bennett ML,Colbert JD,Torres AV,Clayton GH,Ornelles D,Moorhead J

    更新日期：2004-03-02 00:00:00

  • Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery.

    abstract：:A glucose-responsive "closed-loop" insulin delivery system mimicking the function of pancreatic cells has tremendous potential to improve quality of life and health in diabetics. Here, we report a novel glucose-responsive insulin delivery device using a painless microneedle-array patch ("smart insulin patch") containi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1505405112

    authors： Yu J,Zhang Y,Ye Y,DiSanto R,Sun W,Ranson D,Ligler FS,Buse JB,Gu Z

    更新日期：2015-07-07 00:00:00

  • Thymic nurse cells provide microenvironment for secondary T cell receptor α rearrangement in cortical thymocytes.

    abstract：:Distinct subsets of thymic epithelial cells (TECs) support T-cell development and selection. Isolated TECs contain multicellular complexes that enclose many viable thymocytes. However, the functions of those TECs, termed thymic nurse cells (TNCs), are unclear and the idea that TNCs are present in vivo is questioned. H...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1213069109

    authors： Nakagawa Y,Ohigashi I,Nitta T,Sakata M,Tanaka K,Murata S,Kanagawa O,Takahama Y

    更新日期：2012-12-11 00:00:00

  • Overexpression of LMO4 induces mammary hyperplasia, promotes cell invasion, and is a predictor of poor outcome in breast cancer.

    abstract：:The zinc finger protein LMO4 is overexpressed in a high proportion of breast carcinomas. Here, we report that overexpression of a mouse mammary tumor virus (MMTV)-Lmo4 transgene in the mouse mammary gland elicits hyperplasia and mammary intraepithelial neoplasia or adenosquamous carcinoma in two transgenic strains wit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0502990102

    authors： Sum EY,Segara D,Duscio B,Bath ML,Field AS,Sutherland RL,Lindeman GJ,Visvader JE

    更新日期：2005-05-24 00:00:00

  • Localization of Niemann-Pick C1 protein in astrocytes: implications for neuronal degeneration in Niemann- Pick type C disease.

    abstract：:Niemann-Pick type C disease (NP-C) is an inherited neurovisceral lipid storage disorder characterized by progressive neurodegeneration. Most cases of NP-C result from inactivating mutations of NPC1, a recently identified member of a family of genes encoding membrane-bound proteins containing putative sterol sensing do...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.4.1657

    authors： Patel SC,Suresh S,Kumar U,Hu CY,Cooney A,Blanchette-Mackie EJ,Neufeld EB,Patel RC,Brady RO,Patel YC,Pentchev PG,Ong WY

    更新日期：1999-02-16 00:00:00

  • Combinatorial regulation of hepatic cytoplasmic signaling and nuclear transcriptional events by the OGT/REV-ERBα complex.

    abstract：:The nuclear receptor REV-ERBα integrates the circadian clock with hepatic glucose and lipid metabolism by nucleating transcriptional comodulators at genomic regulatory regions. An interactomic approach identified O-GlcNAc transferase (OGT) as a REV-ERBα-interacting protein. By shielding cytoplasmic OGT from proteasoma...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1805397115

    authors： Berthier A,Vinod M,Porez G,Steenackers A,Alexandre J,Yamakawa N,Gheeraert C,Ploton M,Maréchal X,Dubois-Chevalier J,Hovasse A,Schaeffer-Reiss C,Cianférani S,Rolando C,Bray F,Duez H,Eeckhoute J,Lefebvre T,Staels B,Lef

    更新日期：2018-11-20 00:00:00

  • Expression of tandem gene duplicates is often greater than twofold.

    abstract：:Tandem gene duplication is an important mutational process in evolutionary adaptation and human disease. Hypothetically, two tandem gene copies should produce twice the output of a single gene, but this expectation has not been rigorously investigated. Here, we show that tandem duplication often results in more than d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1605886113

    authors： Loehlin DW,Carroll SB

    更新日期：2016-05-24 00:00:00

  • Stat3 and Stat4: members of the family of signal transducers and activators of transcription.

    abstract：:The deduced amino acid sequence of two members of the signal transducers and activators of transcription (STAT) family from the mouse are described. Comparison with the deduced protein sequence of the two previously described genes (Stat91 and Stat113), discovered because of their activation as transcription factors a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.11.4806

    authors： Zhong Z,Wen Z,Darnell JE Jr

    更新日期：1994-05-24 00:00:00

  • In vivo imaging reveals that pregabalin inhibits cortical spreading depression and propagation to subcortical brain structures.

    abstract：:Migraine is characterized by severe headaches that can be preceded by an aura likely caused by cortical spreading depression (SD). The antiepileptic pregabalin (Lyrica) shows clinical promise for migraine therapy, although its efficacy and mechanism of action are unclear. As detected by diffusion-weighted MRI (DW-MRI)...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1614447114

    authors： Cain SM,Bohnet B,LeDue J,Yung AC,Garcia E,Tyson JR,Alles SR,Han H,van den Maagdenberg AM,Kozlowski P,MacVicar BA,Snutch TP

    更新日期：2017-02-28 00:00:00

  • Surfactant protein A mediates mycoplasmacidal activity of alveolar macrophages by production of peroxynitrite.

    abstract：:We have previously shown that surfactant protein A (SP-A) mediates in vitro killing of mycoplasmas by alveolar macrophages (AMs) from resistant C57BL/6 mice through a nitric oxide (.NO)-dependent mechanism. Herein, SP-A-deficient [SP-A(-/-)] and inducible.NO synthase-deficient [iNOS(-/-)] mice were infected intranasal...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.9.4953

    authors： Hickman-Davis J,Gibbs-Erwin J,Lindsey JR,Matalon S

    更新日期：1999-04-27 00:00:00

  • Foreign glycoproteins expressed from recombinant vesicular stomatitis viruses are incorporated efficiently into virus particles.

    abstract：:In a previous study we demonstrated that vesicular stomatitis virus (VSV) can be used as a vector to express a soluble protein in mammalian cells. Here we have generated VSV recombinants that express four different membrane proteins: the cellular CD4 protein, a CD4-G hybrid protein containing the ectodomain of CD4 and...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.21.11359

    authors： Schnell MJ,Buonocore L,Kretzschmar E,Johnson E,Rose JK

    更新日期：1996-10-15 00:00:00