当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Osteoblast regulation via ligand-activated nuclear trafficking of the oxytocin receptor.

Osteoblast regulation via ligand-activated nuclear trafficking of the oxytocin receptor.

Abstract：

:We report that oxytocin (Oxt) receptors (Oxtrs), on stimulation by the ligand Oxt, translocate into the nucleus of osteoblasts, implicating this process in the action of Oxt on osteoblast maturation. Sequential immunocytochemistry of intact cells or isolated nucleoplasts stripped of the outer nuclear membrane showed progressive nuclear localization of the Oxtr; this nuclear translocation was confirmed by monitoring the movement of Oxtr-EGFP as well as by immunogold labeling. Nuclear Oxtr localization was conclusively shown by Western immunoblotting and MS of nuclear lysate proteins. We found that the passage of Oxtrs into the nucleus was facilitated by successive interactions with β-arrestins (Arrbs), the small GTPase Rab5, importin-β (Kpnb1), and transportin-1 (Tnpo1). siRNA-mediated knockdown of Arrb1, Arrb2, or Tnpo1 abrogated Oxt-induced expression of the osteoblast differentiation genes osterix (Sp7), Atf4, bone sialoprotein (Ibsp), and osteocalcin (Bglap) without affecting Erk phosphorylation. Likewise and again, without affecting pErk, inhibiting Arrb recruitment by mutating Ser rich clusters of the nuclear localization signal to Ala abolished nuclear import and Oxtr-induced gene expression. These studies define a previously unidentified mechanism for Oxtr action on bone and open possibilities for direct transcriptional modulation by nuclear G protein-coupled receptors.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Di Benedetto A,Sun L,Zambonin CG,Tamma R,Nico B,Calvano CD,Colaianni G,Ji Y,Mori G,Grano M,Lu P,Colucci S,Yuen T,New MI,Zallone A,Zaidi M

doi

10.1073/pnas.1419349111

subject

Has Abstract

pub_date

2014-11-18 00:00:00

pages

16502-7

issue

46

eissn

0027-8424

issn

1091-6490

pii

1419349111

journal_volume

111

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Engineering antigen-specific primary human NK cells against HER-2 positive carcinomas.

    abstract：:NK cells are promising effectors for tumor adoptive immunotherapy, particularly when considering the targeting of MHC class I low or negative tumors. Yet, NK cells cannot respond to many tumors, which is particularly the case for nonhematopoietic tumors such as carcinomas or melanoma even when these cells lose MHC cla...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0804788105

    authors： Kruschinski A,Moosmann A,Poschke I,Norell H,Chmielewski M,Seliger B,Kiessling R,Blankenstein T,Abken H,Charo J

    更新日期：2008-11-11 00:00:00

  • Grain boundary mobility of carbon in Earth's mantle: a possible carbon flux from the core.

    abstract：:The importance of carbon in Earth's mantle greatly exceeds its modest abundance of approximately 1,000-4,000 ppm. Carbon is a constituent of key terrestrial volatiles (CO, CO(2), CH(4)), it forms diamonds, and it may also contribute to the bulk electrical properties of the silicate Earth. In contrast to that of the ma...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710806105

    authors： Hayden LA,Watson EB

    更新日期：2008-06-24 00:00:00

  • Coupled protein domain motion in Taq polymerase revealed by neutron spin-echo spectroscopy.

    abstract：:Long-range conformational changes in proteins are ubiquitous in biology for the transmission and amplification of signals; such conformational changes can be triggered by small-amplitude, nanosecond protein domain motion. Understanding how conformational changes are initiated requires the characterization of protein d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0503388102

    authors： Bu Z,Biehl R,Monkenbusch M,Richter D,Callaway DJ

    更新日期：2005-12-06 00:00:00

  • A phylogenetic analysis of myosin heavy chain type II sequences corroborates that Acoela and Nemertodermatida are basal bilaterians.

    abstract：:Bilateria are currently subdivided into three superclades: Deuterostomia, Ecdysozoa, and Lophotrochozoa. Within this new taxonomic frame, acoelomate Platyhelminthes, for a long time held to be basal bilaterians, are now considered spiralian lophotrochozoans. However, recent 18S rDNA [small subunit (SSU)] analyses have...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.172390199

    authors： Ruiz-Trillo I,Paps J,Loukota M,Ribera C,Jondelius U,Baguna J,Riutort M

    更新日期：2002-08-20 00:00:00

  • A second wave of Sonic hedgehog expression during the development of the bat limb.

    abstract：:Sonic hedgehog (Shh) plays an integral role in both the anterior-posterior (A-P) patterning and expansion of developing vertebrate limbs through a feedback loop involving Fgfs, Bmps, and Gremlin. In bat limbs A-P patterning and the size of the digital field are unique. The posterior digits of the forelimb are elongate...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0805308105

    authors： Hockman D,Cretekos CJ,Mason MK,Behringer RR,Jacobs DS,Illing N

    更新日期：2008-11-04 00:00:00

  • Histone variants in archaea and the evolution of combinatorial chromatin complexity.

    abstract：:Nucleosomes in eukaryotes act as platforms for the dynamic integration of epigenetic information. Posttranslational modifications are reversibly added or removed and core histones exchanged for paralogous variants, in concert with changing demands on transcription and genome accessibility. Histones are also common in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2007056117

    authors： Stevens KM,Swadling JB,Hocher A,Bang C,Gribaldo S,Schmitz RA,Warnecke T

    更新日期：2020-12-29 00:00:00

  • Modeling the status, trends, and impacts of wild bee abundance in the United States.

    abstract：:Wild bees are highly valuable pollinators. Along with managed honey bees, they provide a critical ecosystem service by ensuring stable pollination to agriculture and wild plant communities. Increasing concern about the welfare of both wild and managed pollinators, however, has prompted recent calls for national evalua...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1517685113

    authors： Koh I,Lonsdorf EV,Williams NM,Brittain C,Isaacs R,Gibbs J,Ricketts TH

    更新日期：2016-01-05 00:00:00

  • Lateral diffusion of surface immunoglobulin, Thy-1 antigen, and a lipid probe in lymphocyte plasma membranes.

    abstract：:Fluorescence photobleaching recovery was used to measure the lateral diffusion coefficient and mobile fraction of surface immunoglobulin (sIg), Thy-1 antigen, and a lipid probe in the plasma membrane of mouse lymphocytes. The lipid probe (3,3'-dioctadecylindocarbocyanine) had a mean (+/-SD) diffusion coefficient of (1...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.10.5163

    authors： Dragsten P,Henkart P,Blumenthal R,Weinstein J,Schlessinger J

    更新日期：1979-10-01 00:00:00

  • Climate change poised to threaten hydrologic connectivity and endemic fishes in dryland streams.

    abstract：:Protecting hydrologic connectivity of freshwater ecosystems is fundamental to ensuring species persistence, ecosystem integrity, and human well-being. More frequent and severe droughts associated with climate change are poised to significantly alter flow intermittence patterns and hydrologic connectivity in dryland st...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1320890111

    authors： Jaeger KL,Olden JD,Pelland NA

    更新日期：2014-09-23 00:00:00

  • In vitro analysis of DNA-protein interactions by proximity ligation.

    abstract：:Protein-binding DNA sequence elements encode a variety of regulated functions of genomes. Information about such elements is currently in a state of rapid growth, but improved methods are required to characterize the sequence specificity of DNA-binding proteins. We have established an in vitro method for specific and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0611229104

    authors： Gustafsdottir SM,Schlingemann J,Rada-Iglesias A,Schallmeiner E,Kamali-Moghaddam M,Wadelius C,Landegren U

    更新日期：2007-02-27 00:00:00

  • Evolution of the rodent eosinophil-associated RNase gene family by rapid gene sorting and positive selection.

    abstract：:The mammalian RNase A superfamily comprises a diverse array of ribonucleolytic proteins that have a variety of biochemical activities and physiological functions. Two rapidly evolving RNases of higher primates are of particular interest as they are major secretory proteins of eosinophilic leukocytes and have been foun...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.080071397

    authors： Zhang J,Dyer KD,Rosenberg HF

    更新日期：2000-04-25 00:00:00

  • Plasminogen activator inhibitor type 1 gene is located at region q21.3-q22 of chromosome 7 and genetically linked with cystic fibrosis.

    abstract：:The regional chromosomal location of the human gene for plasminogen activator inhibitor type 1 (PAI1) was determined by three independent methods of gene mapping. PAI1 was localized first to 7cen-q32 and then to 7q21.3-q22 by Southern blot hybridization analysis of a panel of human and mouse somatic cell hybrids with ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.23.8548

    authors： Klinger KW,Winqvist R,Riccio A,Andreasen PA,Sartorio R,Nielsen LS,Stuart N,Stanislovitis P,Watkins P,Douglas R

    更新日期：1987-12-01 00:00:00

  • Nonfunctional mutants of the retinoblastoma protein are characterized by defects in phosphorylation, viral oncoprotein association, and nuclear tethering.

    abstract：:We have examined the functional consequences of mutations present in defective alleles of the retinoblastoma susceptibility gene (RB1) isolated from two spontaneously arising tumors. Unlike cDNA clones expressing the wild-type protein p110Rb, those encoding the two mutant proteins failed to induce the appearance of se...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.8.3033

    authors： Templeton DJ,Park SH,Lanier L,Weinberg RA

    更新日期：1991-04-15 00:00:00

  • Infiltrating neutrophils mediate the initial angiogenic switch in a mouse model of multistage carcinogenesis.

    abstract：:Matrix metalloprotease type 9 (MMP-9) has been functionally implicated in VEGF activation, the induction and maintenance of chronic angiogenesis, and early stage tumor growth in a number of mouse models of cancer. In this article, we have identified two inflammatory cell types that are major sources of MMP-9 in the an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0601807103

    authors： Nozawa H,Chiu C,Hanahan D

    更新日期：2006-08-15 00:00:00

  • Two distinct inputs to an avian song nucleus activate different glutamate receptor subtypes on individual neurons.

    abstract：:Although neural circuits mediating various simple behaviors have been delineated, those generating more complex behaviors are less well described. The discrete structure of avian song control nuclei promises that circuits controlling complex behaviors, such as birdsong, can also be understood. To this end, we develope...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.10.4075

    authors： Mooney R,Konishi M

    更新日期：1991-05-15 00:00:00

  • Somatic integration of an oncogene-harboring Sleeping Beauty transposon models liver tumor development in the mouse.

    abstract：:The Sleeping Beauty (SB) transposon system can integrate foreign sequences of DNA in the genome of mouse somatic cells eliciting long-term expression in vivo. This technology holds great promise for human gene therapy as a nonviral technology to deliver therapeutic genes. SB also provides a means to study the effects ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0502974102

    authors： Carlson CM,Frandsen JL,Kirchhof N,McIvor RS,Largaespada DA

    更新日期：2005-11-22 00:00:00

  • Defining functional distance using manifold embeddings of gene ontology annotations.

    abstract：:Although rigorous measures of similarity for sequence and structure are now well established, the problem of defining functional relationships has been particularly daunting. Here, we present several manifold embedding techniques to compute distances between Gene Ontology (GO) functional annotations and consequently e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702965104

    authors： Lerman G,Shakhnovich BE

    更新日期：2007-07-03 00:00:00

  • Transcription factor binding predicts histone modifications in human cell lines.

    abstract：:Gene expression in higher organisms is thought to be regulated by a complex network of transcription factor binding and chromatin modifications, yet the relative importance of these two factors remains a matter of debate. Here, we show that a computational approach allows surprisingly accurate prediction of histone mo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1412081111

    authors： Benveniste D,Sonntag HJ,Sanguinetti G,Sproul D

    更新日期：2014-09-16 00:00:00

  • Clustering of solitons in weakly correlated wavefronts.

    abstract：:We demonstrate theoretically and experimentally the spontaneous clustering of solitons in partially coherent wavefronts during the final stages of pattern formation initiated by modulation instability and noise. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.072287299

    authors： Chen Z,Sears SM,Martin H,Christodoulides DN,Segev M

    更新日期：2002-04-16 00:00:00

  • Nuclear-cytoplasmic shuttling of Axin regulates subcellular localization of beta-catenin.

    abstract：:Wnt signaling regulates many aspects of development by increasing the signaling activity of beta-catenin. Axin is a negative regulator of the Wnt signaling pathway, and it is responsible for the formation of the beta-catenin degradation complex. Genetic studies with Drosophila suggest that Axin promotes cytoplasmic lo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0307344101

    authors： Cong F,Varmus H

    更新日期：2004-03-02 00:00:00

  • Fold prediction of helical proteins using torsion angle dynamics and predicted restraints.

    abstract：:We describe a procedure for predicting the tertiary folds of alpha-helical proteins from their primary sequences. The central component of the procedure is a method for predicting interhelical contacts that is based on a helix-packing model. Instead of predicting the individual contacts, our method attempts to identif...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.052003799

    authors： Zhang C,Hou J,Kim SH

    更新日期：2002-03-19 00:00:00

  • Nutritional impact of elevated calcium transport activity in carrots.

    abstract：:Nutrition recommendations worldwide emphasize ingestion of plant-based diets rather than diets that rely primarily on animal products. However, this plant-based diet could limit the intake of essential nutrients such as calcium. Osteoporosis is one of the world's most prevalent nutritional disorders, and inadequate di...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,随机对照试验

    doi：10.1073/pnas.0709005105

    authors： Morris J,Hawthorne KM,Hotze T,Abrams SA,Hirschi KD

    更新日期：2008-02-05 00:00:00

  • Affecting gene expression by altering the length and sequence of the 5' leader.

    abstract：:We have utilized a recombinant vector system designed to study gene control elements by fusing them to the Escherichia coli galactokinase gene (galK) to examine the effects of gene expression that result from introducing changes into the 5' noncoding leader region of the galK transcription unit. We varied the length o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.24.7698

    authors： Johansen H,Schümperli D,Rosenberg M

    更新日期：1984-12-01 00:00:00

  • Quality-control mechanisms targeting translationally stalled and C-terminally extended poly(GR) associated with ALS/FTD.

    abstract：:Maintaining the fidelity of nascent peptide chain (NP) synthesis is essential for proteome integrity and cellular health. Ribosome-associated quality control (RQC) serves to resolve stalled translation, during which untemplated Ala/Thr residues are added C terminally to stalled peptide, as shown during C-terminal Ala ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2005506117

    authors： Li S,Wu Z,Tantray I,Li Y,Chen S,Dong J,Glynn S,Vogel H,Snyder M,Lu B

    更新日期：2020-10-06 00:00:00

  • Hinge region of Arabidopsis phyA plays an important role in regulating phyA function.

    abstract：:Phytochrome A (phyA) is the only plant photoreceptor that perceives far-red light and then mediates various responses to this signal. Phosphorylation and dephosphorylation of oat phyA have been extensively studied, and it was shown that phosphorylation of a serine residue in the hinge region of oat phyA could regulate...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1813162115

    authors： Zhou Y,Yang L,Duan J,Cheng J,Shen Y,Wang X,Han R,Li H,Li Z,Wang L,Terzaghi W,Zhu D,Chen H,Deng XW,Li J

    更新日期：2018-12-11 00:00:00

  • Gammadelta intraepithelial lymphocytes are essential mediators of host-microbial homeostasis at the intestinal mucosal surface.

    abstract：:The mammalian gastrointestinal tract harbors thousands of bacterial species that include symbionts as well as potential pathogens. The immune responses that limit access of these bacteria to underlying tissue remain poorly defined. Here we show that γδ intraepithelial lymphocytes (γδ IEL) of the small intestine produc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1019574108

    authors： Ismail AS,Severson KM,Vaishnava S,Behrendt CL,Yu X,Benjamin JL,Ruhn KA,Hou B,DeFranco AL,Yarovinsky F,Hooper LV

    更新日期：2011-05-24 00:00:00

  • Distinct facial expressions represent pain and pleasure across cultures.

    abstract：:Real-world studies show that the facial expressions produced during pain and orgasm-two different and intense affective experiences-are virtually indistinguishable. However, this finding is counterintuitive, because facial expressions are widely considered to be a powerful tool for social interaction. Consequently, de...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1807862115

    authors： Chen C,Crivelli C,Garrod OGB,Schyns PG,Fernández-Dols JM,Jack RE

    更新日期：2018-10-23 00:00:00

  • Self-removal of condensed water on the legs of water striders.

    abstract：:The ability to control drops and their movements on phobic surfaces is important in printing or patterning, microfluidic devices, and water-repellent materials. These materials are always micro-/nanotextured, and a natural limitation of repellency occurs when drops are small enough (as in a dew) to get trapped in the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1506874112

    authors： Wang Q,Yao X,Liu H,Quéré D,Jiang L

    更新日期：2015-07-28 00:00:00

  • Frequency selectivity of synaptic exocytosis in frog saccular hair cells.

    abstract：:The ability to respond selectively to particular frequency components of sensory inputs is fundamental to signal processing in the ear. The frog (Rana pipiens) sacculus, which is used for social communication and escape behaviors, is an exquisitely sensitive detector of sounds and ground-borne vibrations in the 5- to ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0511005103

    authors： Rutherford MA,Roberts WM

    更新日期：2006-02-21 00:00:00

  • Unit activity in the isolated spinal cord of chick embryo, in situ.

    abstract：:Unit electrical activity was recorded from single neurons in the isolated lumbo-sacral spinal cord of 14- to 19-day chick embryos, in situ. Spinal cord transection was combined with transection of all lumbo-sacral dorsal roots. The spontaneous discharge of cells is confined, for the most part, to the lower two thirds ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.66.1.40

    authors： Sharma SC,Provine RR,Hamburger V,Sandel TT

    更新日期：1970-05-01 00:00:00